Identifiers and Description

Gene Model Identifier

TTHERM_00142330

Standard Name

RAD51 (Homolog of RADiation sensitive protein family 51)

Aliases

PreTt26639 | 11.m00336 | 3702.m00060

Description

RAD51 DNA repair RAD51-like protein; DNA repair protein RAD51 containing protein; strand exchange protein; involved in homologous recombination and repair of double-stranded DNA breaks

Genome Browser (Macronucleus)

Full Screen View

No Genome Browser Data Present

Genome Browser (Micronucleus)

Full Screen View

No Genome Browser Data Present

External Links

• Uniprot
• Alphafold

Gene Ontology Annotations

Cellular Component

• macronucleus (IDA) | GO:0031039

Molecular Function

• ATP binding (IEA) | GO:0005524
• ATP-dependent activity, acting on DNA (IDA) | GO:0008094
• ATP-dependent activity, acting on DNA (IEA) | GO:0008094
• damaged DNA binding (IEA) | GO:0003684
• double-stranded DNA binding (IDA) | GO:0003690
• single-stranded DNA binding (IDA) | GO:0003697

Biological Process

• DNA damage response (IEP) | GO:0006974
• DNA repair (IEA) | GO:0006281
• nuclear division (IMP) | GO:0000280
• regulation of DNA ligation (IDA) | GO:0051105

Domains

• ( PF00154 ) recA protein
• ( PF00633 ) Helix-hairpin-helix motif
• ( PF08423 ) Rad51
• ( TIGR01954 ) transcription termination factor NusA, C-terminal
• ( TIGR02236 ) DNA repair and recombination protein
• ( TIGR02237 ) DNA repair and recombination protein
• ( TIGR02238 ) meiotic recombinase Dmc1
• ( TIGR02239 ) DNA repair protein RAD51

Gene Expression Profile

Vegetative Cell Cycle (Zhang et al., 2023)

GeneMania

No results were calculated for this gene in GeneMania.

Tetrahymena Stock Center

No Data fetched for Tetrahymena Stock Center

Homologs

Source	Identifier	Score
Tetrahymena borealis	EI9_04844.1	9.997093137033517e-262
	Description: DNA repair protein RAD51 containing protein (332 aa)
Oxytricha	Contig22030.0.g96	2.0004882463825047e-175
	Description: Rad51
DictyBase	DDB_G0273611	4.0011977872964304e-148
	Description: rad51-2 on chromosome: 2 position 3169189 to 3170422
SGD	YER095W	1.9991914687114462e-138
	Description: RAD51 Strand exchange protein, forms a helical filament with DNA that searches for homology; involved in the recombinational repair of double-strand breaks in DNA during vegetative growth and meiosis; homolog of Dmc1p and bacterial RecA protein
WormBase	WBGene00004297	2.998573346093879e-125
	Description: locus:rad-51 status:Confirmed UniProt:L8E822 protein_id:CCQ25700.1
Stentor Coeruleus	SteCoe_12930	3.5722699376192174e-67
	Description: None

General Information

No Data fetched for General Information

Associated Literature

1. Ref:34086947: Nabeel-Shah S, Garg J, Saettone A, Ashraf K, Lee H, Wahab S, Ahmed N, Fine J, Derynck J, Pu S, Ponce M, Marcon E, Zhang Z, Greenblatt JF, Pearlman RE, Lambert JP, Fillingham J (2021) Functional characterization of RebL1 highlights the evolutionary conservation of oncogenic activities of the RBBP4/7 orthologue in Tetrahymena thermophila. Nucleic acids research ( ):
2. Ref:21483758: Howard-Till RA, Lukaszewicz A, Loidl J (2011) The recombinases Rad51 and Dmc1 play distinct roles in DNA break repair and recombination partner choice in the meiosis of Tetrahymena. PLoS genetics 7(3):e1001359
3. Ref:17005912: Yakisich JS, Sandoval PY, Morrison TL, Kapler GM (2006) TIF1 activates the intra-S-phase checkpoint response in the diploid micronucleus and amitotic polyploid macronucleus of Tetrahymena. Molecular biology of the cell 17(12):5185-97
4. Ref:15304567: Smith JJ, Cole ES, Romero DP (2004) Transcriptional control of RAD51 expression in the ciliate Tetrahymena thermophila. Nucleic acids research 32(14):4313-21
5. Ref:15522890: Loidl J, Scherthan H (2004) Organization and pairing of meiotic chromosomes in the ciliate Tetrahymena thermophila. Journal of cell science 117(Pt 24):5791-801
6. Ref:11290715: Marsh TC, Cole ES, Romero DP (2001) The transition from conjugal development to the first vegetative cell division is dependent on RAD51 expression in the ciliate Tetrahymena thermophila. Genetics 157(4):1591-8
7. Ref:10747055: Marsh TC, Cole ES, Stuart KR, Campbell C, Romero DP (2000) RAD51 is required for propagation of the germinal nucleus in Tetrahymena thermophila. Genetics 154(4):1587-96
8. Ref:9628914: Campbell C, Romero DP (1998) Identification and characterization of the RAD51 gene from the ciliate Tetrahymena thermophila. Nucleic acids research 26(13):3165-72

Sequences

>TTHERM_00142330(coding)
ATGGCTGAGTACGCTGAAGACGTTGAATAAAGCGATGGACCTATGTTGATCGCCAAATTG
GAAGAACATGGTATTAATAATGCAGATGTTAAAAAACTCATAGATGCTGGTTTCCAAACT
GTTGAATCCATTTCTTACACTGCTAAGAAAAATCTCTTGCAAATTAAGGGTATGACTGAA
GCAAAAATCGATAAAATCCTTGATGTTGCTGCCAAATTAGTTCCTAATGATTTCTAAACT
GCAGCTGAATACTATGTAAAGAGACAAAGTGTCATCAATCTTACTACAGGCTCAACTGAA
TTAGACAAACTTCTTGGTGGTGGTTTTGAAACAGGCTCTCTCACTGAAATTTTTGGTGAA
TTCAGAACAGGTAAAACTTAAATTTGTCATACTTTGTGTATCACCTGCTAATTACCCAAG
GAAAAGGGTGGTGGTGAAGGTAAGGCTATGTATATTGATACTGAAGGTACTTTCAGACCT
GAAAGACTTGAATCAATTGCTGAAAGATTCGGATTAGATCCTCAAGAATGTATGGAAAAC
GTAGCTTATGCTAGAGCCTTCAACTGTGATCAATAAAATAAACTCTTAGTCCAGGCTGCT
GCTTTGATGGCTGAAAGTAAATACGCTTTGCTTATTGTTGATTCAGCCACTGCTCTTTAC
AGAACTGATTATAGTGGTAGAGGTGAATTGTCAGTCAGACAAAATCACTTGGGTAAATTC
TTGAGAAACTTATAAAGACTTGCTGACGAATTCGGTATTGCTGTAGTTATTACCAACCAA
GTTATGTCCTAAGTCGATGGTGCAGCTATGTTCGCTGGTGATATGAAGAAACCTATTGGT
GGTAATATTATGGCTCACGCTTCTACTACCAGACTTTATCTCAGAAAAGGTAGAGGAGAG
TCTCGTATTTGCAAAATTTACGATTCTCCATGTCTTCCTGAATCAGAAGCTATCTACGCT
ATTGGTAAGGGTGGTATTGAAGACTTCAACGAGTGA

>TTHERM_00142330(gene)
ATGGCTGAGTACGCTGAAGACGTTGAATAAAGCGATGGACCTATGTTGATCGCCAAATTG
GAAGAACATGGTATTAATAATGCAGATGTTAAAAAACTCATAGATGCTGGTTTCCAAACT
GTTGAATCCATTTCTTACACTGCTAAGAAAAATCTCTTGCAAATTAAGGGTATGACTGAA
GCAAAAATCGATAAAATCCTTGATGTTGCTGCCAAATTAGTTCCTAATGATTTCTAAACT
GCAGCTGAATACTATGTAAAGAGACAAAGTGTCATCAATCTTACTACAGGCTCAACTGAA
TTAGACAAACTTCTTGGTGGTGGTTTTGAAACAGGCTCTCTCACTGAAATTTTTGGTAAA
AATATTAATTAATTATTTGTTTATAAAATAGAATAATAAGTTTAAGAAATTAATATCAAA
TTTTAGCCACCATTTCTTTTTAAATATGATTTCCATCCATATTTATCATTAATTCTTAAA
AAATAGGTGAATTCAGAACAGGTAAAACTTAAATTTGTCATACTTTGTGTATCACCTGCT
AATTACCCAAGGAAAAGGGTGGTGGTGAAGGTAAGGCTATGTATATTGATACTGAAGGTA
CTTTCAGACCTGAAAGACTTGAATCAATTGCTGAAAGATTCGGATTAGATCCTCAAGAAT
GTATGGAAAACGTAGCTTATGCTAGAGCCTTCAACTGTGATCAATAAAATAAACTCTTAG
TCCAGGCTGCTGCTTTGATGGCTGAAAGTAAATACGCTTTGCTTATTGTTGATTCAGCCA
CTGCTCTTTACAGAACTGATTATAGTGGTAGAGGTGAATTGTCAGTCAGACAAAATCACT
TGGGTAAATTCTTGAGAAACTTATAAAGACTTGCTGACGAATTCGGTATTGCTGTAGTTA
TTACCAACCAAGTTATGTCCTAAGTCGATGGTGCAGCTATGTTCGCTGGTGATATGAAGA
AACCTATTGGTGGTAATATTATGGCTCACGCTTCTACTACCAGACTTTATCTCAGAAAAG
GTAGAGGAGAGTCTCGTATTTGCAAAATTTACGATTCTCCATGTCTTCCTGAATCAGAAG
TAATTCACTCATTCATTTATTAAATATATTTTTGCTTTGAAATAAATACACAAAAGTGTG
AGCCAAATAAATACGAATAATTTATTTTATCAATTTATATTAGAAAAAAAATACTTTATT
AAAAAATTTATTAATAATATTTGTTTAACTATAGGCTATCTACGCTATTGGTAAGGGTGG
TATTGAAGACTTCAACGAGTGA

>TTHERM_00142330(protein)
MAEYAEDVEQSDGPMLIAKLEEHGINNADVKKLIDAGFQTVESISYTAKKNLLQIKGMTE
AKIDKILDVAAKLVPNDFQTAAEYYVKRQSVINLTTGSTELDKLLGGGFETGSLTEIFGE
FRTGKTQICHTLCITCQLPKEKGGGEGKAMYIDTEGTFRPERLESIAERFGLDPQECMEN
VAYARAFNCDQQNKLLVQAAALMAESKYALLIVDSATALYRTDYSGRGELSVRQNHLGKF
LRNLQRLADEFGIAVVITNQVMSQVDGAAMFAGDMKKPIGGNIMAHASTTRLYLRKGRGE
SRICKIYDSPCLPESEAIYAIGKGGIEDFNE