Identifiers and Description

Gene Model Identifier

TTHERM_00191480

Standard Name

ASI2 (Anlagen Stage Induced 2)

Aliases

PreTt08799 | 15.m00510 | 3697.m00221

Description

ASI2 anlagen stage induced 1 protein; Similar to anlagen stage induced protein 1 (ASI1); protein of unknown function; gene is located adjacent to the ASI1 gene

Genome Browser (Macronucleus)

Full Screen View


Genome Browser (Micronucleus)

Full Screen View

External Links

Gene Ontology Annotations

Cellular Component

Molecular Function

Biological Process

Domains

  • ( PF00672 ) HAMP domain
  • ( PF02326 ) YMF19 hypothetical plant mitochondrial protein
  • ( PF02743 ) Cache domain

Gene Expression Profile

Vegetative Cell Cycle (Zhang et al., 2023)

GeneMania

Tetrahymena Stock Center

  • ( SD01594 ) Macronucleus: ASI2 replaced with neo2 cassette
  • ( SD01595 ) Macronucleus: ASI2 replaced with neo2 cassette
  • ( SD01596 )
  • ( SD01597 )
  • ( SD03069 ) Micronucleus: heterozygous for ASI2 knockout
  • ( SD03070 ) Micronucleus: ASI2
  • ( SD03071 ) Macronucleus: ASI2 somatic knockout
  • ( SD03072 ) Macronucleus: ASI2 knockout
  • ( SD03073 ) Macronucleus: ASI2 replaced with ASI2Y607F

Homologs

Source Identifier Score
Tetrahymena borealis EI9_06168.1 9.997093137033517e-262
Description: ASI2 protein (724 aa)

General Information

No. Gene Name(s) Paragraph Text
85 ASI2 Gene Information: ASI2 is a gene regulating endocycling in Tetrahymena thermophila. Though nonessential for vegative growth, it is upregulated after meiosis and is involved in the creation of a new MAC. Introduced via transduction with ASI2-GFP plasmid to establish parental cells that showed transcription of ASI2 occurs both in MAC and parental cells at conjugation.
86 ASI2 Nucleic Acid Interactions: ASI2p independent of ssRNA synthesis/accumulation
87 ASI2 Regulation & Expression: The Tetrahymena gene ASI1 (anlagen stage induced 1) was isolated from a cDNA library of genes that are up-regulated during development of the macronuclear anlagen. As its name implies, the abundance of ASI2 mRNA peaks at 9 h of mating, early in macronuclear anlagen development.

Associated Literature

  1. Ref:20656911: Yin L, Gater ST, Karrer KM (2010) A developmentally regulated gene, ASI2, is required for endocycling in the macronuclear anlagen of Tetrahymena. Eukaryotic cell 9(9):1343-53
  2. Ref:16712831: Li S, Yin L, Cole ES, Udani RA, Karrer KM (2006) Progeny of germ line knockouts of ASI2, a gene encoding a putative signal transduction receptor in Tetrahymena thermophila, fail to make the transition from sexual reproduction to vegetative growth. Developmental biology 295(2):633-46

Sequences 

>TTHERM_00191480(coding)
ATGACACAAACGGAACTCGTTCCTGAAGATTATGACCAGGATAATTAAGATGCTTCTCAA
AGCGTAATCTCTTCAGGAAGCGTTGAAGATGGAAAAGAAAAATTTAAAGCAATGATACAT
AAAATTTTAGGAGTAAACGATAACAAATAAATAGATAAAATATGCTGTTTCTCCATTTTT
AATAAGCAGTGGTCTCGTACAAAGCAACTGTTTTGTGTTTACTCAGTCTTTTTGACATTA
TTTATGACTATAATATTAGGGTGTTTTGTGTTCTACTTAAAATTTTGTCCCAATTTCGTA
GGAGATACAATCTTAGAGTAGATTGATAACTCAATTTCTGCAAGCTTTTAAAATTCCTTA
CTTGAATACAGTAAATAAGTACATTAATATTTCAAAGCATATAGTTCTTGGATGATTATG
TTCTCCCAAATAGTAGCTAACAATGTTGGTTGGAATAATTCACTATATTAATCCTCAAAC
GAATTAAATTTATAACCACAACAAAAATATTTTCCTATTTATTAGCCTATCCCATAAAAT
ATAGTAAATAAGTAATATTCTTAGAAAAAGCCCTATTATGATAAAGATATATCATTTTAA
ACTTTTTCTTATTTTCACAACCATCCTGACGATGCAACTACTATTTCACCAGCTACTTAA
ACATATTTGGACTCTGTAGACTTGATTTCTTATTTTTTTTCTAATTTTATATAAGATCCT
AATTTAACTTATGTCTAGTCTTTATATATTTACCTTTAAGATCGAGATGTCATTTGTTTT
TTTCCAGCAGGCCTCATTAAAGTCCCAAAAGATTTTAAGATAGATATGAAATAAAGGCCT
TGGTATTTAAATACTGCTTAAACATTTTAAGCCAATGGAAATAAACCTGTATTTACAATT
ACCAGTCCCTATCCTGATTTAACTACTAAATAAATAACTATAACATACTCCCTTGCAATC
GTCAAACAGTCAGATCCTACTTAAATTGCTGGAGTTATTAATGTAGATGTTATTGTTGGT
AATTTTAGATCTGTTTATGACTTGCTGCAGGAAAAAGATGGTGAATTTTAAATTGCCTTA
GTAGATTATAATACAGAATACATCATTAATGATTGCTTTAATTAAAGTGGAATTTCACAT
TTTTATGATACGAATAATACAAACTTTACACTTGAAAATTGGAATGAAATCAAAGATTTT
AATTAACACCAATAAAGTTAAACATTCATCATTACTAATAGTTAGAATGTAAGATATTAT
TTTGCTCGTACTTTAATAAAAGATACAGTAAATCAAACATTAAATAGATTTTATCTTATC
GTCTTTGGTAAAGAAAGCAATTATCTCTCCTATAGAAACAATATTTAAGATAGAATATAT
AATGAGCTCTATAACTTTTGCATATCATTAATAATTGCTTTTTCTATTTTGTGGTTCTTT
TCATATTTCTATATCTATGCTCTATCACGCAATATTGTTCTACCGCTTTTTAAATTGAGT
TAATTAGCTAAAAGTATAAACAAGCACGATGAGAGAAAAAGCTCTGCTGAAGACTTGAAA
GAATAGTTAGGTAAGATTTAAGGTGCAGATACTGATACAAAAAAGCTTATCGATTCTTTC
ATTAACCTTGTAGATTAGCACTCTGGCATGAGAAAACTAGCAAATGGTAATGGTTACTAA
GACAATATGATCCCAGTCTACAATAGAATGAGCAACATTTATGAAAACTATTCTAGGAAT
GACTACAGAGAAGAATTGGATGATATATAAGAATAAATTAAATAATTAAAGAATTTAGAT
TAGGAAGATGAAGATGATTATGATGAAAATGGATCTAATTAAGATATTGATGAAGTCTAT
GCTGGAAATCCTAAAAATAAATCTTATTCATCAATTTAATCTAATAAATATTAAAACGAA
GATGATTAAACAGAAGCTGATCTTAGAAATCATAAAAAAAAGTTAGAGTTAGAAGCGAAA
GGAGGCAACCGTACTTCAACAAATTCTTTAGCTAGTAATTTAGAAAACTATAATAAAAGA
AAATATCTAAATTGGATTTAAACTTAACTCTAAGTAATACAAGAAAACGAATAAGCTAAA
ATAAAGTTGTCTCAATAAATTTTATAGTAATTTTTACCTACAAATACTTAATCTATTTGA


>TTHERM_00191480(gene)
ATGACACAAACGGAACTCGTTCCTGAAGATTATGACCAGGATAATTAAGATGCTTCTCAA
AGCGTAATCTCTTCAGGAAGCGTTGAAGATGGAAAAGAAAAATTTAAAGCAATGATACAT
AAAATTTTAGGAGTAAACGATAACAAATAAATAGATAAAATATGCTGTTTCTCCATTTTT
AATAAGCAGTGGTCTCGTACAAAGCAACTGTTTTGTGTTTACTCAGTCTTTTTGACATTA
TTTATGACTATAATATTAGGGTGTTTTGTGTTCTACTTAAAATTTTGTCCCAATTTCGTA
GGAGATACAATCTTAGAGTAGATTGATAACTCAATTTCTGCAAGCTTTTAAAATTCCTTA
CTTGAATACAGTAAATAAGTACATTAATATTTCAAAGCATATAGTTCTTGGATGATTATG
TTCTCCCAAATAGTAGCTAACAATGTTGGTTGGAATAATTCACTATATTAATCCTCAAAC
GAATTAAATTTATAACCACAACAAAAATATTTTCCTATTTATTAGCCTATCCCATAAAAT
ATAGTAAATAAGTAATATTCTTAGAAAAAGCCCTATTATGATAAAGATATATCATTTTAA
ACTTTTTCTTATTTTCACAACCATCCTGACGATGCAACTACTATTTCACCAGCTACTTAA
ACATATTTGGACTCTGTAGACTTGATTTCTTATTTTTTTTCTAATTTTATATAAGATCCT
AATTTAACTTATGTCTAGTCTTTATATATTTACCTTTAAGATCGAGATGTCATTTGTTTT
TTTCCAGCAGGCCTCATTAAAGTCCCAAAAGATTTTAAGATAGATATGAAATAAAGGCCT
TGGTATTTAAATACTGCTTAAACATTTTAAGCCAATGGAAATAAACCTGTATTTACAATT
ACCAGTCCCTATCCTGATTTAACTACTAAATAAATAACTATAACATACTCCCTTGCAATC
GTCAAACAGGTAATTCATAAATAAATCAATATTTCTATTATTTTTAATTTTGACAATTTA
AAATGCAAATAACTAAATAATAATATTTATTTATTTATTTATTTGCTTTTTGATTTTTCA
GTGGGTTAATTAATTTAAAATTTTTAATTAAAATAGTCAGATCCTACTTAAATTGCTGGA
GTTATTAATGTAGATGTTATTGTTGGTAATTTTAGATCTGTTTATGACTTGCTGCAGGAA
AAAGATGGTGAATTTTAAATTGCCTTAGTAGATTATAATACAGAATACATCATTAATGAT
TGCTTTAATTAAAGTGGAATTTCACATTTTTATGATACGAATAATACAAACTTTACACTT
GAAAATTGGAATGAAATCAAAGATTTTAATTAACACCAATAAAGTTAAACATTCATCATT
ACTAATAGTTAGAATGTAAGATATTATTTTGCTCGTACTTTAATAAAAGATACAGTAAAT
CAAACATTAAATAGATTTTATCTTATCGTCTTTGGTAAAGAAAGCAATTATCTCTCCTAT
AGAAACAATATTTAAGATAGAATATATAATGAGCTCTATAACTTTTGCATATCATTAATA
ATTGCTTTTTCTATTTTGTGGTTCTTTTCATATTTCTATATCTATGCTCTATCACGCAAT
ATTGTTCTACCGCTTTTTAAATTGAGTTAATTAGCTAAAAGTATAAACAAGCACGATGAG
AGAAAAAGCTCTGCTGAAGACTTGAAAGAATAGTTAGGTAAGATTTAAGGTGCAGATACT
GATACAAAAAAGCTTATCGATTCTTTCATTAACCTTGTAGATTAGCACTCTGGCATGAGA
AAACTAGCAAATGGTAATGGTTACTAAGACAATATGATCCCAGTCTACAATAGAATGAGC
AACATTTATGAAAACTATTCTAGGAATGACTACAGAGAAGAATTGGATGATATATAAGAA
TAAATTAAATAATTAAAGAATTTAGATTAGGAAGATGAAGATGATTATGATGAAAATGGA
TCTAATTAAGATATTGATGAAGTCTATGCTGGAAATCCTAAAAATAAATCTTATTCATCA
ATTTAATCTAATAAATATTAAAACGAAGATGATTAAACAGAAGCTGATCTTAGAAATCAT
AAAAAAAAGTTAGAGTTAGAAGCGAAAGGAGGCAACCGTACTTCAACAAATTCTTTAGCT
AGTAATTTAGAAAACTATAATAAAAGAAAATATCTAAATTGGATTTAAACTTAACTCTAA
GTAATACAAGAAAACGAATAAGCTAAAATAAAGTTGTCTCAATAAATTTTATAGTAATTT
TTACCTACAAATACTTAATCTATTTGA


>TTHERM_00191480(protein)
MTQTELVPEDYDQDNQDASQSVISSGSVEDGKEKFKAMIHKILGVNDNKQIDKICCFSIF
NKQWSRTKQLFCVYSVFLTLFMTIILGCFVFYLKFCPNFVGDTILEQIDNSISASFQNSL
LEYSKQVHQYFKAYSSWMIMFSQIVANNVGWNNSLYQSSNELNLQPQQKYFPIYQPIPQN
IVNKQYSQKKPYYDKDISFQTFSYFHNHPDDATTISPATQTYLDSVDLISYFFSNFIQDP
NLTYVQSLYIYLQDRDVICFFPAGLIKVPKDFKIDMKQRPWYLNTAQTFQANGNKPVFTI
TSPYPDLTTKQITITYSLAIVKQSDPTQIAGVINVDVIVGNFRSVYDLLQEKDGEFQIAL
VDYNTEYIINDCFNQSGISHFYDTNNTNFTLENWNEIKDFNQHQQSQTFIITNSQNVRYY
FARTLIKDTVNQTLNRFYLIVFGKESNYLSYRNNIQDRIYNELYNFCISLIIAFSILWFF
SYFYIYALSRNIVLPLFKLSQLAKSINKHDERKSSAEDLKEQLGKIQGADTDTKKLIDSF
INLVDQHSGMRKLANGNGYQDNMIPVYNRMSNIYENYSRNDYREELDDIQEQIKQLKNLD
QEDEDDYDENGSNQDIDEVYAGNPKNKSYSSIQSNKYQNEDDQTEADLRNHKKKLELEAK
GGNRTSTNSLASNLENYNKRKYLNWIQTQLQVIQENEQAKIKLSQQILQQFLPTNTQSI