Identifiers and Description

Gene Model Identifier

TTHERM_00049080

Standard Name

POB3 (POl1 Binding)

Aliases

PreTt11287 | 3.m01985 | 3824.m03045 | Ssrp1

Description

POB3 structure-specific recognition protein; Structure-specific recognition protein (Ssrp1). Interacts with Spt16 (Cet1) as a component of the FACT-complex (Ashraf et al. 2019).

Genome Browser (Macronucleus)

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Genome Browser (Micronucleus)

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Gene Ontology Annotations

Molecular Function

Biological Process

  • negative regulation of protein kinase activity (IEA) | GO:0006469

Domains

No Data fetched for Domains

Gene Expression Profile

Tetrahymena Stock Center

No Data fetched for Tetrahymena Stock Center

Homologs

Source Identifier Score
Tetrahymena borealis EI9_01649.1 9.997093137033517e-262
Description: structure-specific recognition protein (437 aa)
Oxytricha Contig22582.0.g104 6.001629673589058e-65
Description: Structure-specific recognition protein (SSRP1)
SGD YML069W 2.999948199478294e-54
Description: POB3 Subunit of the heterodimeric FACT complex (Spt16p-Pob3p), which associates with chromatin via interaction with Nhp6Ap and Nhp6Bp, and reorganizes nucleosomes to facilitate access to DNA by RNA and DNA polymerases
WormBase WBGene00001974 3.998479955315009e-46
Description: locus:hmg-4 FACT complex subunit SSRP1-A status:Confirmed UniProt:P41848 protein_id:CCD69078.1
DictyBase DDB_G0290331 3.998777993244535e-41
Description: ssrp1 on chromosome: 5 position 3969916 to 3971699
Stentor Coeruleus SteCoe_35967 1.462486227251231e-31
Description: None

General Information

No. Gene Name(s) Paragraph Text
129 POB3 The FACT-complex is a critical transcription regulator and an H2A/H2B chaperone. Affinity purification followed by mass spectrometry (AP-MS) of endogenously tagged T. thermophila histones H2A/H2B identified Cet1 and Pob3 subunits of the FACT complex as high-confidence interacting partners. Indirect immunofluorescence studies using endogenously tagged Cet1 indicated that it localized to both the MAC and MIC during vegetative growth. AP-MS analysis using Tetrahymena Cet1 as a bait protein revealed Pob3 and subunits of RNA polymerase as high-confidence co-purifying proteins (Ashraf et al. 2019).

Associated Literature

  1. Ref:30796450: Ashraf K, Nabeel-Shah S, Garg J, Saettone A, Derynck J, Gingras AC, Lambert JP, Pearlman RE, Fillingham J (2019) Proteomic analysis of histones H2A/H2B and variant Hv1 in Tetrahymena thermophila reveals an ancient network of chaperones. Molecular biology and evolution ( ):

Sequences 

>TTHERM_00049080(coding)
ATGAGTGATAGGTGTTATAAAGGTGTCAACTGGGGTAATGTTACCTTTGAAGATGACTAA
TTAGTCCTTAGTCATAACAAGAGAAGATTGTGCAAACTTTCTTTAAAGAAATTCACAAAC
TCTACTGTTAACAAAACTGATATTGTAATTGATTTGAACACAGTTGATCTTCAAGATGAT
GAAGACTAACTCTGTGAAATGAGATTATTTATTCCTCAATAATAAGACGCTTAAATGAAA
CCTGAAGATGATGGAGAAGGTGAAGAAAAAGAAGGTGAAGATAGCGTAGGTGGATATGCA
GATTAACTTAACTCTGAAATTATTACTAAAGCTAAAATTGGCTAATACTCTGGTTAATCA
ATAGTTAAATTCGAAGACATATCACTTCTTGTTCCTAGAGGTAAATACCAACTTGATATG
TACAAAAAAACAGTAAGATTCCACGGCTCTTCTTTTAACTATATAGTAGAATATAGTAAT
GTTATTAAGGGCTTCCTCCTTCCTCAACCTGACGAAGTACATGTTGCTTTCGTTCTTGGT
TTAGATTAACCCTTAAAAATTGGTAATACTGTTCACTCTTACATAGTTATGCAATTCAAA
AAAGAACAAAAGGCTAACATCAAAGTAAACATTGATCCTGAAGAAAAGAAGGAAGATAAA
TTAAAGGATCTCGATGAAGAATATGATGGATTCTTATATGAAATTGCTGGTTAATTGTTT
AAAACTTTATGTAATAATGTATAAATCATCATGCCTGCTGGTTTCTAAAGCTCTGATAAG
TAAAATTGCTTGAAATGTACCCTTAAAACTCACCAAGGTCTTTTATATCCTATGAGAAAG
AGTTTGATCTTTATATATAAACCTGTAATTCACATATAAATCAGTGATATTTAAAAGGTC
GAATTTAATAGAGTTGGTAATGCTACTCTGAATAAATTGTTTGATGTCAAAGTATTTACT
AAGACCACAACCCCTCAATTCTTTGGTTTTGAAAGAAAGGAATTAGATGTCCTCTTAGAA
TACTTTAAATCTAAAAATATTAAGATTACATATGATGATACTAATTAAGGAGCCACTTTT
GATGATGAGGATGAGGAATTCACAGACTCTATTTCTGAAGATGAGGAAGAAGGTAAGAGA
GCTCAAAGAAAGAGTGCTAAGAGAGCTAATAAAGCAGTAAAAGGATAATCAGGATTACCC
CTTGACGATGATGACGAAGAAGAAGACGAAGACTTTGATGCTGATGAATTTGAAAGTGAA
GATGATGAGGAAGAGGAAGAAGACGAAGATGAAGATGAAGATTTTGAATAATGA


>TTHERM_00049080(gene)
ATGAGTGATAGGTGTTATAAAGGTGTCAACTGGGGTAATGTTACCTTTGAAGATGACTAA
TTAGTCCTTAGTCATAACAAGAGAAGATTGTGCAAACTTTCTTTAAAGAAATTCACAAAC
TCTACTGTTAACAAAACTGATATTGTAATTGATTTGAACACAGTTGATCTTCAAGATGAG
TATCTTCATTTTTTTATTTTCCTCTAGATAGAAAAAAGATTCAAAAAATGTTTATTTTTA
CTTCAATTTTTGAAAAATAATGAATAAATTTACACAGAAAAGCCATTTTAGCTAATATTT
TATGCATATAATTATAGTGAAGACTAACTCTGTGAAATGAGATTATTTATTCCTCAATAA
TAAGACGCTGTAAAAATTAGTATTTAGTTAATGTAATTTTCTCAAAAACAATGTATTAAT
TCAAAAGTAAATGAAACCTGAAGATGATGGAGAAGGTGAAGAAAAAGAAGGTGAAGATAG
CGTAGGTGGATATGCAGATTAACTTAACTCTGAAATTATTACTAAAGCTAAAATTGGCTA
ATACTCTGGTTAATCAATAGTTAAATTCGAAGACATATCACTTCTTGTTCCTAGGTAAAT
TTTAAAACAAATTATCTAATTAGCTTCACATGCATTATGTTATACCACTTCCTAGGCCCA
ATAATATAAAATAATAAGTTTAAAAGTTTAAACATTATGAAAAATTGGCGAATAGTTTGC
TTAGAAGTTATAGCATTTTTTATATATATAAAATAATATGTCTATATTAAGAAATAGGAT
TATGACAAAATTTTAGCAGACTTTATTTAAATCTTAAAATTTTATAAATATAATAGAGGT
AAATACCAACTTGATATGTACAAAAAAACAGTAAGATTCCACGGCTCTTCTTTTAACTAT
ATAGTAGAATATAGTAATGTTATTAAGGGCTTCCTCCTTCCTCAACCTGACGAAGTAAGA
AGATTAAAAAGAGTTATAAGACAATTATAAATCATATTTTAAAAAAGGTACATGTTGCTT
TCGTTCTTGGTTTAGATTAACCCTTAAAAATTGGTAATACTGTTCACTCTTACATAGTTA
TGCAATTCAAAAAAGAACAAAAGGCTAACATCAAAGTAAACATTGATCCTGAAGAAAAGA
AGGAAGATAAATTAAAGGATCTCGATGAAGAATATGATGGATTCTTATATGAAATTGCTG
GTTAATTGTTTAAAACTTTATGTAATAATGTATAAATCATCATGCCTGCTGGTTTCTAAA
GGTAAACAATTAATTTTTTATTTTAGTTGAGTTAAATATAATTTAATAAATAACTAGCTC
TGATAAGTAAAATTGCTTGAAATGTACCCTTAAAACTCACCAAGGTCTTTTATATCCTAT
GAGAAAGAGTTTGATCTTTATATATAAACCTGTAATTCACATATAAATCAGTGATATTTA
AAAGGTCGAATTTAATAGAGTAAAATGAAATTTTTTGTCTAGAAATATTTTATTGAACAA
TTTGCCAAATAATAAAAAAAAATAGGTTGGTAATGCTACTCTGAATAAATTGTTTGATGT
CAAAGTATTTACTAAGACCACAACCCCTCAATTCTTTGGTTTTGAAAGAAAGGAATTAGA
TGTCCTCTTAGAATACTTTAAATCTAAAAATATTAAGATTACATATGATGATACTAATTA
AGGAGCCACTTTTGATGATGAGGATGAGGAATTCACAGACTCTATTTCTGAAGATGAGGA
AGAAGTATAATTTATCTTTTTATTTATTTCTGATAAAAATTTATATTGTAATTAAAGGGT
AAGAGAGCTCAAAGAAAGAGTGCTAAGAGAGCTAATAAAGCAGTAAAAGGATAATCAGGA
TTACCCCTTGACGATGATGACGAAGAAGAAGACGAAGACTTTGATGCTGATGAATTTGAA
AGTGAAGATGATGAGGAAGAGGAAGAAGACGAAGATGAAGATGAAGATTTTGAATAATGA


>TTHERM_00049080(protein)
MSDRCYKGVNWGNVTFEDDQLVLSHNKRRLCKLSLKKFTNSTVNKTDIVIDLNTVDLQDD
EDQLCEMRLFIPQQQDAQMKPEDDGEGEEKEGEDSVGGYADQLNSEIITKAKIGQYSGQS
IVKFEDISLLVPRGKYQLDMYKKTVRFHGSSFNYIVEYSNVIKGFLLPQPDEVHVAFVLG
LDQPLKIGNTVHSYIVMQFKKEQKANIKVNIDPEEKKEDKLKDLDEEYDGFLYEIAGQLF
KTLCNNVQIIMPAGFQSSDKQNCLKCTLKTHQGLLYPMRKSLIFIYKPVIHIQISDIQKV
EFNRVGNATLNKLFDVKVFTKTTTPQFFGFERKELDVLLEYFKSKNIKITYDDTNQGATF
DDEDEEFTDSISEDEEEGKRAQRKSAKRANKAVKGQSGLPLDDDDEEEDEDFDADEFESE
DDEEEEEDEDEDEDFEQ