Identifiers and Description

Gene Model Identifier

TTHERM_00191480

Standard Name

ASI2 (Anlagen Stage Induced 2)

Aliases

PreTt08799 | 15.m00510 | 3697.m00221

Description

ASI2 anlagen stage induced 1 protein; Similar to anlagen stage induced protein 1 (ASI1); protein of unknown function; gene is located adjacent to the ASI1 gene; Periplasmic sensor-like domain superfamily

Genome Browser (Macronucleus)

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Genome Browser (Micronucleus)

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External Links

Gene Ontology Annotations

Cellular Component

Molecular Function

Biological Process

Domains

No Data fetched for Domains

Gene Expression Profile

Vegetative Cell Cycle (Zhang et al., 2023)

GeneMania

Tetrahymena Stock Center

  • ( SD01594 ) Macronucleus: ASI2 replaced with neo2 cassette
  • ( SD01595 ) Macronucleus: ASI2 replaced with neo2 cassette
  • ( SD01596 )
  • ( SD01597 )
  • ( SD03069 ) Micronucleus: heterozygous for ASI2 knockout
  • ( SD03070 ) Micronucleus: ASI2
  • ( SD03071 ) Macronucleus: ASI2 somatic knockout
  • ( SD03072 ) Macronucleus: ASI2 knockout
  • ( SD03073 ) Macronucleus: ASI2 replaced with ASI2Y607F

Homologs

No Data fetched for Homologs

General Information

No. Gene Name(s) Paragraph Text
2291 ASI2 Gene Information: ASI2 is a gene regulating endocycling in Tetrahymena thermophila. Though nonessential for vegative growth, it is upregulated after meiosis and is involved in the creation of a new MAC. Introduced via transduction with ASI2-GFP plasmid to establish parental cells that showed transcription of ASI2 occurs both in MAC and parental cells at conjugation.
2292 ASI2 Nucleic Acid Interactions: ASI2p independent of ssRNA synthesis/accumulation
2293 ASI2 Regulation & Expression: The Tetrahymena gene ASI1 (anlagen stage induced 1) was isolated from a cDNA library of genes that are up-regulated during development of the macronuclear anlagen. As its name implies, the abundance of ASI2 mRNA peaks at 9 h of mating, early in macronuclear anlagen development.

Associated Literature

  1. Ref:20656911: Yin L, Gater ST, Karrer KM (2010) A developmentally regulated gene, ASI2, is required for endocycling in the macronuclear anlagen of Tetrahymena. Eukaryotic cell 9(9):1343-53
  2. Ref:16712831: Li S, Yin L, Cole ES, Udani RA, Karrer KM (2006) Progeny of germ line knockouts of ASI2, a gene encoding a putative signal transduction receptor in Tetrahymena thermophila, fail to make the transition from sexual reproduction to vegetative growth. Developmental biology 295(2):633-46

Sequences 

>TTHERM_00191480(coding)
ATGACACAAACGGAACTCGTTCCTGAAGATTATGACCAGGATAATTAAGATGCTTCTCAA
AGCGTAATCTCTTCAGGAAGCGTTGAAGATGGAAAAGAAAAATTTAAAGCAATGATACAT
AAAATTTTAGGAGTAAACGATAACAAATAAATAGATAAAATATGCTGTTTCTCCATTTTT
AATAAGCAGTGGTCTCGTACAAAGCAACTGTTTTGTGTTTACTCAGTCTTTTTGACATTA
TTTATGACTATAATATTAGGGTGTTTTGTGTTCTACTTAAAATTTTGTCCCAATTTCGTA
GGAGATACAATCTTAGAGTAGATTGATAACTCAATTTCTGCAAGCTTTTAAAATTCCTTA
CTTGAATACAGTAAATAAGTACATTAATATTTCAAAGCATATAGTTCTTGGATGATTATG
TTCTCCCAAATAGTAGCTAACAATGTTGGTTGGAATAATTCACTATATTAATCCTCAAAC
GAATTAAATTTATAACCACAACAAAAATATTTTCCTATTTATTAGCCTATCCCATAAAAT
ATAGTAAATAAGTAATATTCTTAGAAAAAGCCCTATTATGATAAAGATATATCATTTTAA
ACTTTTTCTTATTTTCACAACCATCCTGACGATGCAACTACTATTTCACCAGCTACTTAA
ACATATTTGGACTCTGTAGACTTGATTTCTTATTTTTTTTCTAATTTTATATAAGATCCT
AATTTAACTTATGTCTAGTCTTTATATATTTACCTTTAAGATCGAGATGTCATTTGTTTT
TTTCCAGCAGGCCTCATTAAAGTCCCAAAAGATTTTAAGATAGATATGAAATAAAGGCCT
TGGTATTTAAATACTGCTTAAACATTTTAAGCCAATGGAAATAAACCTGTATTTACAATT
ACCAGTCCCTATCCTGATTTAACTACTAAATAAATAACTATAACATACTCCCTTGCAATC
GTCAAACAGTCAGATCCTACTTAAATTGCTGGAGTTATTAATGTAGATGTTATTGTTGGT
AATTTTAGATCTGTTTATGACTTGCTGCAGGAAAAAGATGGTGAATTTTAAATTGCCTTA
GTAGATTATAATACAGAATACATCATTAATGATTGCTTTAATTAAAGTGGAATTTCACAT
TTTTATGATACGAATAATACAAACTTTACACTTGAAAATTGGAATGAAATCAAAGATTTT
AATTAACACCAATAAAGTTAAACATTCATCATTACTAATAGTTAGAATGTAAGATATTAT
TTTGCTCGTACTTTAATAAAAGATACAGTAAATCAAACATTAAATAGATTTTATCTTATC
GTCTTTGGTAAAGAAAGCAATTATCTCTCCTATAGAAACAATATTTAAGATAGAATATAT
AATGAGCTCTATAACTTTTGCATATCATTAATAATTGCTTTTTCTATTTTGTGGTTCTTT
TCATATTTCTATATCTATGCTCTATCACGCAATATTGTTCTACCGCTTTTTAAATTGAGT
TAATTAGCTAAAAGTATAAACAAGCACGATGAGAGAAAAAGCTCTGCTGAAGACTTGAAA
GAATAGTTAGGTAAGATTTAAGGTGCAGATACTGATACAAAAAAGCTTATCGATTCTTTC
ATTAACCTTGTAGATTAGCACTCTGGCATGAGAAAACTAGCAAATGGTAATGGTTACTAA
GACAATATGATCCCAGTCTACAATAGAATGAGCAACATTTATGAAAACTATTCTAGGAAT
GACTACAGAGAAGAATTGGATGATATATAAGAATAAATTAAATAATTAAAGAATTTAGAT
TAGGAAGATGAAGATGATTATGATGAAAATGGATCTAATTAAGATATTGATGAAGTCTAT
GCTGGAAATCCTAAAAATAAATCTTATTCATCAATTTAATCTAATAAATATTAAAACGAA
GATGATTAAACAGAAGCTGATCTTAGAAATCATAAAAAAAAGTTAGAGTTAGAAGCGAAA
GGAGGCAACCGTACTTCAACAAATTCTTTAGCTAGTAATTTAGAAAACTATAATAAAAGA
AAATATCTAAATTGGATTTAAACTTAACTCTAAGTAATACAAGAAAACGAATAAGCTAAA
ATAAAGTTGTCTCAATAAATTTTATAGTAATTTTTACCTACAAATACTTAATCTATTTGA




>TTHERM_00191480(gene)
CAAAATTTACTATAATTTTATTTTTAAATAAATGGTTGATTTATTCATTAATTAATTATG
TGATTGCGCCTATGCTTTTGCAAATTTTTAATACAATTCCTAAAATTTTTTGCAATTTAT
TGTTTTTAATTCAAGTAGTAAATAATCTCTTTAAAATTATTACAGAAATAGGCAAGCAAA
TAAGACAAATTATAACAGGCTATTCTCTGGAAAAATATAAAATATAACTGATAGGACAAT
ACCATTTTGGACCGCGTATATAGCAATATAATTTAAATTTACATTAAGACATAGATTATT
TACTAGGCAATACGATTTTGCTACTGCTTTTCAAATAATTATTTAGATATTGTTCAAGCA
TATCCGATTAGATTATTCATTCAATTAATTTTTAAGCATAGGGACATAACAGAATTTAGC
TTTTGATCATCATAAAGGAAAGGTGAAAAATCAGAAAAAGCATTATTCTTATCAAAAAGA
TTTTAAGCTGGAAAGTCATTTGGAGTGGATTTTAAAATTGATTAGTTATGACACAAACGG
AACTCGTTCCTGAAGATTATGACCAGGATAATTAAGATGCTTCTCAAAGCGTAATCTCTT
CAGGAAGCGTTGAAGATGGAAAAGAAAAATTTAAAGCAATGATACATAAAATTTTAGGAG
TAAACGATAACAAATAAATAGATAAAATATGCTGTTTCTCCATTTTTAATAAGCAGTGGT
CTCGTACAAAGCAACTGTTTTGTGTTTACTCAGTCTTTTTGACATTATTTATGACTATAA
TATTAGGGTGTTTTGTGTTCTACTTAAAATTTTGTCCCAATTTCGTAGGAGATACAATCT
TAGAGTAGATTGATAACTCAATTTCTGCAAGCTTTTAAAATTCCTTACTTGAATACAGTA
AATAAGTACATTAATATTTCAAAGCATATAGTTCTTGGATGATTATGTTCTCCCAAATAG
TAGCTAACAATGTTGGTTGGAATAATTCACTATATTAATCCTCAAACGAATTAAATTTAT
AACCACAACAAAAATATTTTCCTATTTATTAGCCTATCCCATAAAATATAGTAAATAAGT
AATATTCTTAGAAAAAGCCCTATTATGATAAAGATATATCATTTTAAACTTTTTCTTATT
TTCACAACCATCCTGACGATGCAACTACTATTTCACCAGCTACTTAAACATATTTGGACT
CTGTAGACTTGATTTCTTATTTTTTTTCTAATTTTATATAAGATCCTAATTTAACTTATG
TCTAGTCTTTATATATTTACCTTTAAGATCGAGATGTCATTTGTTTTTTTCCAGCAGGCC
TCATTAAAGTCCCAAAAGATTTTAAGATAGATATGAAATAAAGGCCTTGGTATTTAAATA
CTGCTTAAACATTTTAAGCCAATGGAAATAAACCTGTATTTACAATTACCAGTCCCTATC
CTGATTTAACTACTAAATAAATAACTATAACATACTCCCTTGCAATCGTCAAACAGTCAG
ATCCTACTTAAATTGCTGGAGTTATTAATGTAGATGTTATTGTTGGTAATTTTAGATCTG
TTTATGACTTGCTGCAGGAAAAAGATGGTGAATTTTAAATTGCCTTAGTAGATTATAATA
CAGAATACATCATTAATGATTGCTTTAATTAAAGTGGAATTTCACATTTTTATGATACGA
ATAATACAAACTTTACACTTGAAAATTGGAATGAAATCAAAGATTTTAATTAACACCAAT
AAAGTTAAACATTCATCATTACTAATAGTTAGAATGTAAGATATTATTTTGCTCGTACTT
TAATAAAAGATACAGTAAATCAAACATTAAATAGATTTTATCTTATCGTCTTTGGTAAAG
AAAGCAATTATCTCTCCTATAGAAACAATATTTAAGATAGAATATATAATGAGCTCTATA
ACTTTTGCATATCATTAATAATTGCTTTTTCTATTTTGTGGTTCTTTTCATATTTCTATA
TCTATGCTCTATCACGCAATATTGTTCTACCGCTTTTTAAATTGAGTTAATTAGCTAAAA
GTATAAACAAGCACGATGAGAGAAAAAGCTCTGCTGAAGACTTGAAAGAATAGTTAGGTA
AGATTTAAGGTGCAGATACTGATACAAAAAAGCTTATCGATTCTTTCATTAACCTTGTAG
ATTAGCACTCTGGCATGAGAAAACTAGCAAATGGTAATGGTTACTAAGACAATATGATCC
CAGTCTACAATAGAATGAGCAACATTTATGAAAACTATTCTAGGAATGACTACAGAGAAG
AATTGGATGATATATAAGAATAAATTAAATAATTAAAGAATTTAGATTAGGAAGATGAAG
ATGATTATGATGAAAATGGATCTAATTAAGATATTGATGAAGTCTATGCTGGAAATCCTA
AAAATAAATCTTATTCATCAATTTAATCTAATAAATATTAAAACGAAGATGATTAAACAG
AAGCTGATCTTAGAAATCATAAAAAAAAGTTAGAGTTAGAAGCGAAAGGAGGCAACCGTA
CTTCAACAAATTCTTTAGCTAGTAATTTAGAAAACTATAATAAAAGAAAATATCTAAATT
GGATTTAAACTTAACTCTAAGTAATACAAGAAAACGAATAAGCTAAAATAAAGTTGTCTC
AATAAATTTTATAGTAATTTTTACCTACAAATACTTAATCTATTTGACTAACACTCACAC
TAACACACACACATTTTAAATTATAAGCAAATTTCTATAAATTAATTCGAAATGTGAATC
AAAAGCATTTAACAAAATAAGCTTTGGTAAACAAAAAAGTGTATGTTTTAAACTAACCAA
AAATATAAATAAATTAATTTTCAAAAGTTTACTCAACAAAGTCATAATAGTTTAATTAAT
AGTTTTCATAAATCATATAAAAATACAATCAATCAATAAATAAATATTTAAAGTAATATA
TTTCAAAAATTTTCCCTAAAATACAAAAAATAAATAATGAATTTAATTATTTGTCAGGGA
TTTTATAAATAAATAAATACTCACCTATCTATATATTAATCAATATAATTTTAAACTTTC
TTTTGTAATGAATTTGTATTTTCAGTTTTATTTTAACAAATAAAAATCTTAGTTAAAGTC
TATAGTTTAAATTGAATTATTTTTAATAAA



>TTHERM_00191480(protein)
MTQTELVPEDYDQDNQDASQSVISSGSVEDGKEKFKAMIHKILGVNDNKQIDKICCFSIF
NKQWSRTKQLFCVYSVFLTLFMTIILGCFVFYLKFCPNFVGDTILEQIDNSISASFQNSL
LEYSKQVHQYFKAYSSWMIMFSQIVANNVGWNNSLYQSSNELNLQPQQKYFPIYQPIPQN
IVNKQYSQKKPYYDKDISFQTFSYFHNHPDDATTISPATQTYLDSVDLISYFFSNFIQDP
NLTYVQSLYIYLQDRDVICFFPAGLIKVPKDFKIDMKQRPWYLNTAQTFQANGNKPVFTI
TSPYPDLTTKQITITYSLAIVKQSDPTQIAGVINVDVIVGNFRSVYDLLQEKDGEFQIAL
VDYNTEYIINDCFNQSGISHFYDTNNTNFTLENWNEIKDFNQHQQSQTFIITNSQNVRYY
FARTLIKDTVNQTLNRFYLIVFGKESNYLSYRNNIQDRIYNELYNFCISLIIAFSILWFF
SYFYIYALSRNIVLPLFKLSQLAKSINKHDERKSSAEDLKEQLGKIQGADTDTKKLIDSF
INLVDQHSGMRKLANGNGYQDNMIPVYNRMSNIYENYSRNDYREELDDIQEQIKQLKNLD
QEDEDDYDENGSNQDIDEVYAGNPKNKSYSSIQSNKYQNEDDQTEADLRNHKKKLELEAK
GGNRTSTNSLASNLENYNKRKYLNWIQTQLQVIQENEQAKIKLSQQILQQFLPTNTQSI