Identifiers and Description

Gene Model Identifier

TTHERM_00653810

Standard Name

TWI12 (Tetrahymena piWI 12)

Aliases

PreTt12868 | 95.m00114 | 3825.m02522

Description

TWI12 piwi domain protein; Argonaute/Piwi family protein; PAZ domain

Genome Browser (Macronucleus)

Full Screen View

No Genome Browser Data Present

Genome Browser (Micronucleus)

Full Screen View

No Genome Browser Data Present

External Links

• Uniprot
• Alphafold

Gene Ontology Annotations

Molecular Function

• tRNA binding (IDA) | GO:0000049

Biological Process

• tRNA decay (IDA) | GO:0016078

Domains

• ( PF02170 ) PAZ domain

Gene Expression Profile

Vegetative Cell Cycle (Zhang et al., 2023)

GeneMania

No results were calculated for this gene in GeneMania.

Tetrahymena Stock Center

No Data fetched for Tetrahymena Stock Center

Homologs

No Data fetched for Homologs

General Information

No.	Gene Name(s)	Paragraph Text
2265	TWI12	Gene Model error: The open reading frame for the major protein product starts at an upstream in-frame ATG, adding 17 amino acids to the N-terminus of the predicted protein (Couvillion et al., Mol. Cell, 2012).

Associated Literature

1. Ref:23084833: Couvillion MT, Bounova G, Purdom E, Speed TP, Collins K (2012) A Tetrahymena Piwi bound to mature tRNA 3' fragments activates the exonuclease Xrn2 for RNA processing in the nucleus. Molecular cell 48(4):509-20
2. Ref:21106669: Couvillion MT, Sachidanandam R, Collins K (2010) A growth-essential Tetrahymena Piwi protein carries tRNA fragment cargo. Genes & development 24(24):2742-7
3. Ref:19656801: Couvillion MT, Lee SR, Hogstad B, Malone CD, Tonkin LA, Sachidanandam R, Hannon GJ, Collins K (2009) Sequence, biogenesis, and function of diverse small RNA classes bound to the Piwi family proteins of Tetrahymena thermophila. Genes & development 23(17):2016-32

Sequences

>TTHERM_00653810(coding)
ATGGATCAGCAATAGATTTATAAGTTATTTTTGTATTCCAATCTTTTGAAGATGGATATA
CAATCACCCATTTATAAGCAAGATGTTTAGATAACTCCTGAAAAATTTGCTTCTAGTTCA
GATAGAGAAGCTGTATTCTTGGGAGTGCGCAAAATATTACATATCTCTCATGATATGGAC
AATTGTATTTTTTTAAAGAAAAACAATATTCACCTTAGTGAAGAAGTTTAAGAGAATCCA
TAAGCACAATGGGTTTGCTATAGCAGTAAATAAATGTACTAAGGTTAGGATAGTATGAGT
GTTTTAGGATACTAGGTTAGTATTAAATAGATTTAACAAAATGGTAGTCCTGCATTTATA
ACTACCAACAAAGAGAGATAGCTTGTTTTAAATTATATTGCAAGAGAAATTTTTTCAAAA
CTAAATTATCATTAATACTAAAAAGGGTTTTTCAATCCTATTGACCCCCCTAATGCTGCC
CTTAGTAAAGATAAAACTGTTGTTTATCAAACTTACTCATGGAATGGGCTAAGACCTAAG
GCAATTGAATTTAGAGGTAAAGTTTATCTGGAAATTGAAACCACTCCTGTGCGCTTCTTG
CGTATGAATTTGTTCTAAATTATTAACAAACTTGAAGATAAAAAAATTCCAAGAGAAGAA
ATAAGCAATTTTTTGGTAGGACGCACTGTTAAGACATCTTATTTCTCCACTAATCTATAT
TATGAAATTTTAGAAATAGATTTTAATATGACTCCTGACAGTAAATTTGTCGTAAAATAA
AACTAAATTTAAGATGAAGGAGATATGATTGCAGAAAAAGGTGATGAAGAACTCTCTTAT
TATTCTTATGTTAAGAACAAATATAGAATTAACATTAGAGACAGAGTTTAGCCAATGATT
AGAGTTCGCCCTCTTCACAAAGGCTTTAAATCCGAATTTCATAGTAGTTAAGATTAGCAA
ATGTACCTCATTCCAAGTCTTTGCAATCTTTTTGGATAGATTTCTCAAAAAGGTTTTATT
GACGATAATGTTGGAACAGAATTCTACTTTGAACCTTATGATGTATATGATTATACTAAT
TAAATTACTAGATTTTTAAAAAATAGCTCTGAAGCTTAAAGTTTGCTAAATGGATGGTAA
ATTAAAATAGGAGAAGAGTTCGAACAGATTGGTTTTAACTACCTCAAATTATCTCCTTTA
TAACTCAAAGTTAATAATGTCAATGAGTCTTTTTCTCCTAACGACTTATGCTGTAAGCGT
TTATGTAACGAATTCGATGGATATTATAAATAGCTATTCTGTTCTGATGGAGCTTTCACT
AAATTCACTGAAGTTAATAGATGGGTTATTTTTGTCCACTCTAGCGATTCACCATTAGTT
GAAGCTCTGGTCTCTTCGATCTCTCAGTTTAAGCTATACTACAATTACCCTATTTCAGAA
CCAAAATATTATAAGATAGATGATTTAAATATTGAAACATGGACAAGCACCTTTAATACT
GCCTTATCTGAGAGTCCTACTACATTTGCATTGATTATTATGAACAGTGAATTAGAGCAC
TATCACTTTTATAAAACTTGTAAGCAAGCAATTACAGTGGATAAAGGCATTTGCTGTTCA
CTTATTAAGGCTTCTACTCTCTAGCAATATGTTCCTACTTAATAATATCCATAAATACCC
CCAGGAAACTCGATTGAAGCTTTTGCAAGCCGTTTCTTATCATAAATGCAAGCTAAGCTT
GGTGAGTCACCTTGGCTTATGTAGGAATTACCATACAATTAAAATCCTTGCACAATGATA
GGGTTTAGTGTATTACGCCAACGTAATGCTAGAAAAATTATAGTAGCAGGAGCTGCTTCT
ATTAATAAATAGTATAGCAAATATCTCCCTGATTATAGAGTTATTAGTGAAGTTTCAACT
AAAATCAAGGAAGATGGTACTTTAGAGATAGTAGAGCTTTCAGAACAAGAAATAAACAAT
TCAATAACTAATAGCATGAAAGATATTCTTCAAGGATTTTTAAGTTAGTTTGCAACAACT
CACAAAATAAATCCAACCTGGGTTATTATTATTCGTGACGGTATATTTATTTAAAATGCA
CAAAATGAAAAAGATGCTATATTCTCTATGATGAGCACTCGCCGTTCCTAAAGTGAAAAC
AAATCATTCCACCTTTTGTTTGCAACATCTCGTTATCAAACTAGCTTCCGTACTTATAAT
TTCTATGGATCATCAGAACTTAATGCTAACACATAAAACGTATCTTCTCCAGGAACAGGT
GCTATAGTTGAAAAGTAGTTGAACAAGCGCGAATTTTATCTTTATACATAAAAAATAGTT
AAAAACTGTCCATTTTCTGTGCTATACAATGTATTACATATAGATAAATTTATTGGATTA
GACGAGCATTCTTTGTTAGCTGATTTCTAAGACTTGGTATATAACACTTATGTGCTAAAT
TATACATACCACACAAAGACAACTTCACCAGCTTGTGTACAAAATGCTTATAAATTAGCA
GAATTTGTTAAAATGTTTGTAACACCTGCAGGGTCTTAATAAAATACAATAGATCTATCA
AACTTGAGAGTGCTGTGCTCAAATTATAAGCTCTTCTTCATTTGA

>TTHERM_00653810(gene)
TATATGTTTGCAAAAAGAAAATATATATTGTTCAAAGATATTAAAAAATTATTTAATTAG
TTAAGAAAGCAAAATTAGTAGACTAATAAATTATAAAATCAAGATAAAGCAATATTTATT
TTACATAAATGGATCAGCAATAGATTTATAAGTTATTTTTGTATTCCAATCTTTTGAAGA
TGGATATACAATCACCCATTTATAAGCAAGATGTTTAGATAACTCCTGAAAAATTTGCTT
CTAGTTCAGATAGAGAAGCTGTATTCTTGGGAGTGCGCAAAATATTACATATCTCTCATG
ATATGGACAATTGTATTTTTTTAAAGAAAAACAATATTCACCTTAGTGAAGAAGTTTAAG
AGAATCCATAAGCACAATGGGTTTGCTATAGCAGTAAATAAATGTACTAAGGTTAGGATA
GTATGAGTGTTTTAGGATACTAGGTTAGTATTAAATAGATTTAACAAAATGGTAGTCCTG
CATTTATAACTACCAACAAAGAGAGATAGCTTGTTTTAAATTATATTGCAAGAGAAATTT
TTTCAAAACTAAATTATCATTAATACTAAAAAGGGTTTTTCAATCCTATTGACCCCCCTA
ATGCTGCCCTTAGTAAAGATAAAACTGTTGTTTATCAAACTTACTCATGGAATGGGCTAA
GACCTAAGGCAATTGAATTTAGAGGTAAAGTTTATCTGGAAATTGAAACCACTCCTGTGC
GCTTCTTGCGTATGAATTTGTTCTAAATTATTAACAAACTTGAAGATAAAAAAATTCCAA
GAGAAGAAATAAGCAATTTTTTGGTAGGACGCACTGTTAAGACATCTTATTTCTCCACTA
ATCTATATTATGAAATTTTAGAAATAGATTTTAATATGACTCCTGACAGTAAATTTGTCG
TAAAATAAAACTAAATTTAAGATGAAGGAGATATGATTGCAGAAAAAGGTGATGAAGAAC
TCTCTTATTATTCTTATGTTAAGAACAAATATAGAATTAACATTAGAGACAGAGTTTAGC
CAATGATTAGAGTTCGCCCTCTTCACAAAGGCTTTAAATCCGAATTTCATAGTAGTTAAG
ATTAGCAAATGTACCTCATTCCAAGTCTTTGCAATCTTTTTGGATAGATTTCTCAAAAAG
GTTTTATTGACGATAATGTTGGAACAGAATTCTACTTTGAACCTTATGATGTATATGATT
ATACTAATTAAATTACTAGATTTTTAAAAAATAGCTCTGAAGCTTAAAGTTTGCTAAATG
GATGGTAAATTAAAATAGGAGAAGAGTTCGAACAGATTGGTTTTAACTACCTCAAATTAT
CTCCTTTATAACTCAAAGTTAATAATGTCAATGAGTCTTTTTCTCCTAACGACTTATGCT
GTAAGCGTTTATGTAACGAATTCGATGGATATTATAAATAGCTATTCTGTTCTGATGGAG
CTTTCACTAAATTCACTGAAGTTAATAGATGGGTTATTTTTGTCCACTCTAGCGATTCAC
CATTAGTTGAAGCTCTGGTCTCTTCGATCTCTCAGTTTAAGCTATACTACAATTACCCTA
TTTCAGAACCAAAATATTATAAGATAGATGATTTAAATATTGAAACATGGACAAGCACCT
TTAATACTGCCTTATCTGAGAGTCCTACTACATTTGCATTGATTATTATGAACAGTGAAT
TAGAGCACTATCACTTTTATAAAACTTGTAAGCAAGCAATTACAGTGGATAAAGGCATTT
GCTGTTCACTTATTAAGGCTTCTACTCTCTAGCAATATGTTCCTACTTAATAATATCCAT
AAATACCCCCAGGAAACTCGATTGAAGCTTTTGCAAGCCGTTTCTTATCATAAATGCAAG
CTAAGCTTGGTGAGTCACCTTGGCTTATGTAGGAATTACCATACAATTAAAATCCTTGCA
CAATGATAGGGTTTAGTGTATTACGCCAACGTAATGCTAGAAAAATTATAGTAGCAGGAG
CTGCTTCTATTAATAAATAGTATAGCAAATATCTCCCTGATTATAGAGTTATTAGTGAAG
TTTCAACTAAAATCAAGGAAGATGGTACTTTAGAGATAGTAGAGCTTTCAGAACAAGAAA
TAAACAATTCAATAACTAATAGCATGAAAGATATTCTTCAAGGATTTTTAAGTTAGTTTG
CAACAACTCACAAAATAAATCCAACCTGGGTTATTATTATTCGTGACGGTATATTTATTT
AAAATGCACAAAATGAAAAAGATGCTATATTCTCTATGATGAGCACTCGCCGTTCCTAAA
GTGAAAACAAATCATTCCACCTTTTGTTTGCAACATCTCGTTATCAAACTAGCTTCCGTA
CTTATAATTTCTATGGATCATCAGAACTTAATGCTAACACATAAAACGTATCTTCTCCAG
GAACAGGTGCTATAGTTGAAAAGTAGTTGAACAAGCGCGAATTTTATCTTTATACATAAA
AAATAGTTAAAAACTGTCCATTTTCTGTGCTATACAATGTATTACATATAGATAAATTTA
TTGGATTAGACGAGCATTCTTTGTTAGCTGATTTCTAAGACTTGGTATATAACACTTATG
TGCTAAATTATACATACCACACAAAGACAACTTCACCAGCTTGTGTACAAAATGCTTATA
AATTAGCAGAATTTGTTAAAATGTTTGTAACACCTGCAGGGTCTTAATAAAATACAATAG
ATCTATCAAACTTGAGAGTGCTGTGCTCAAATTATAAGCTCTTCTTCATTTGATTAATTT
TTAAAAAATATAAAACCTTACTAAATAAATAAATACATAGTTAATAATTAGCGAATTTAT
GTTTAAATATTAGATTTGTAATTGTTTTATTTTAGATTTAGTAAAAAAA

>TTHERM_00653810(protein)
MDQQQIYKLFLYSNLLKMDIQSPIYKQDVQITPEKFASSSDREAVFLGVRKILHISHDMD
NCIFLKKNNIHLSEEVQENPQAQWVCYSSKQMYQGQDSMSVLGYQVSIKQIQQNGSPAFI
TTNKERQLVLNYIAREIFSKLNYHQYQKGFFNPIDPPNAALSKDKTVVYQTYSWNGLRPK
AIEFRGKVYLEIETTPVRFLRMNLFQIINKLEDKKIPREEISNFLVGRTVKTSYFSTNLY
YEILEIDFNMTPDSKFVVKQNQIQDEGDMIAEKGDEELSYYSYVKNKYRINIRDRVQPMI
RVRPLHKGFKSEFHSSQDQQMYLIPSLCNLFGQISQKGFIDDNVGTEFYFEPYDVYDYTN
QITRFLKNSSEAQSLLNGWQIKIGEEFEQIGFNYLKLSPLQLKVNNVNESFSPNDLCCKR
LCNEFDGYYKQLFCSDGAFTKFTEVNRWVIFVHSSDSPLVEALVSSISQFKLYYNYPISE
PKYYKIDDLNIETWTSTFNTALSESPTTFALIIMNSELEHYHFYKTCKQAITVDKGICCS
LIKASTLQQYVPTQQYPQIPPGNSIEAFASRFLSQMQAKLGESPWLMQELPYNQNPCTMI
GFSVLRQRNARKIIVAGAASINKQYSKYLPDYRVISEVSTKIKEDGTLEIVELSEQEINN
SITNSMKDILQGFLSQFATTHKINPTWVIIIRDGIFIQNAQNEKDAIFSMMSTRRSQSEN
KSFHLLFATSRYQTSFRTYNFYGSSELNANTQNVSSPGTGAIVEKQLNKREFYLYTQKIV
KNCPFSVLYNVLHIDKFIGLDEHSLLADFQDLVYNTYVLNYTYHTKTTSPACVQNAYKLA
EFVKMFVTPAGSQQNTIDLSNLRVLCSNYKLFFI