Identifiers and Description

Gene Model Identifier

TTHERM_00653810

Standard Name

TWI12 (Tetrahymena piWI 12)

Aliases

PreTt12868 | 95.m00114 | 3825.m02522

Description

TWI12 piwi domain protein; Argonaute/Piwi family protein

Genome Browser (Macronucleus)

Full Screen View

No Genome Browser Data Present

Genome Browser (Micronucleus)

Full Screen View

No Genome Browser Data Present

Gene Ontology Annotations

Biological Process

• indole metabolic process (IDA) | GO:0042431 | Ref:21106669
• ribosomal small subunit export from nucleus (IDA) | GO:0000056 | Ref:21106669

Domains

• ( TIGR01100 ) V-type ATPase, C subunit

Gene Expression Profile

Tetrahymena Stock Center

No Data fetched for Tetrahymena Stock Center

Homologs

Source	Identifier	Score
Tetrahymena borealis	EI9_00722.1	9.997093137033517e-262
	Description: PAZ domain-containing protein (881 aa)
Oxytricha	Contig276.0.g87	6.002718307283067e-26
	Description: Piwi domain
Stentor Coeruleus	SteCoe_15480	0.0000000001026187963170189
	Description: None
WormBase	WBGene00004179	0.000000000500206551404915
	Description: locus:prg-2 status:Partially_confirmed UniProt:Q17567 protein_id:CCD62443.1
DictyBase	DDB_G0289367	0.000000019990673443141236
	Description: agnE on chromosome: 5 position 2710003 to 2713807

General Information

No.	Gene Name(s)	Paragraph Text
59	TWI12	Gene Model error: The open reading frame for the major protein product starts at an upstream in-frame ATG, adding 17 amino acids to the N-terminus of the predicted protein (Couvillion et al., Mol. Cell, 2012).

Associated Literature

1. Ref:23084833: Couvillion MT, Bounova G, Purdom E, Speed TP, Collins K (2012) A Tetrahymena Piwi bound to mature tRNA 3' fragments activates the exonuclease Xrn2 for RNA processing in the nucleus. Molecular cell 48(4):509-20
2. Ref:21106669: Couvillion MT, Sachidanandam R, Collins K (2010) A growth-essential Tetrahymena Piwi protein carries tRNA fragment cargo. Genes & development 24(24):2742-7
3. Ref:19656801: Couvillion MT, Lee SR, Hogstad B, Malone CD, Tonkin LA, Sachidanandam R, Hannon GJ, Collins K (2009) Sequence, biogenesis, and function of diverse small RNA classes bound to the Piwi family proteins of Tetrahymena thermophila. Genes & development 23(17):2016-32

Sequences

>TTHERM_00653810(coding)
ATGGATCAGCAATAGATTTATAAGTTATTTTTGTATTCCAATCTTTTGAAGATGGATATA
CAATCACCCATTTATAAGCAAGATGTTTAGATAACTCCTGAAAAATTTGCTTCTAGTTCA
GATAGAGAAGCTGTATTCTTGGGAGTGCGCAAAATATTACATATCTCTCATGATATGGAC
AATTGTATTTTTTTAAAGAAAAACAATATTCACCTTAGTGAAGAAGTTTAAGAGAATCCA
TAAGCACAATGGGTTTGCTATAGCAGTAAATAAATGTACTAAGGTTAGGATAGTATGAGT
GTTTTAGGATACTAGGTTAGTATTAAATAGATTTAACAAAATGGTAGTCCTGCATTTATA
ACTACCAACAAAGAGAGATAGCTTGTTTTAAATTATATTGCAAGAGAAATTTTTTCAAAA
CTAAATTATCATTAATACTAAAAAGGGTTTTTCAATCCTATTGACCCCCCTAATGCTGCC
CTTAGTAAAGATAAAACTGTTGTTTATCAAACTTACTCATGGAATGGGCTAAGACCTAAG
GCAATTGAATTTAGAGGTAAAGTTTATCTGGAAATTGAAACCACTCCTGTGCGCTTCTTG
CGTATGAATTTGTTCTAAATTATTAACAAACTTGAAGATAAAAAAATTCCAAGAGAAGAA
ATAAGCAATTTTTTGGTAGGACGCACTGTTAAGACATCTTATTTCTCCACTAATCTATAT
TATGAAATTTTAGAAATAGATTTTAATATGACTCCTGACAGTAAATTTGTCGTAAAATAA
AACTAAATTTAAGATGAAGGAGATATGATTGCAGAAAAAGGTGATGAAGAACTCTCTTAT
TATTCTTATGTTAAGAACAAATATAGAATTAACATTAGAGACAGAGTTTAGCCAATGATT
AGAGTTCGCCCTCTTCACAAAGGCTTTAAATCCGAATTTCATAGTAGTTAAGATTAGCAA
ATGTACCTCATTCCAAGTCTTTGCAATCTTTTTGGATAGATTTCTCAAAAAGGTTTTATT
GACGATAATGTTGGAACAGAATTCTACTTTGAACCTTATGATGTATATGATTATACTAAT
TAAATTACTAGATTTTTAAAAAATAGCTCTGAAGCTTAAAGTTTGCTAAATGGATGGTAA
ATTAAAATAGGAGAAGAGTTCGAACAGATTGGTTTTAACTACCTCAAATTATCTCCTTTA
TAACTCAAAGTTAATAATGTCAATGAGTCTTTTTCTCCTAACGACTTATGCTGTAAGCGT
TTATGTAACGAATTCGATGGATATTATAAATAGCTATTCTGTTCTGATGGAGCTTTCACT
AAATTCACTGAAGTTAATAGATGGGTTATTTTTGTCCACTCTAGCGATTCACCATTAGTT
GAAGCTCTGGTCTCTTCGATCTCTCAGTTTAAGCTATACTACAATTACCCTATTTCAGAA
CCAAAATATTATAAGATAGATGATTTAAATATTGAAACATGGACAAGCACCTTTAATACT
GCCTTATCTGAGAGTCCTACTACATTTGCATTGATTATTATGAACAGTGAATTAGAGCAC
TATCACTTTTATAAAACTTGTAAGCAAGCAATTACAGTGGATAAAGGCATTTGCTGTTCA
CTTATTAAGGCTTCTACTCTCTAGCAATATGTTCCTACTTAATAATATCCATAAATACCC
CCAGGAAACTCGATTGAAGCTTTTGCAAGCCGTTTCTTATCATAAATGCAAGCTAAGCTT
GGTGAGTCACCTTGGCTTATGTAGGAATTACCATACAATTAAAATCCTTGCACAATGATA
GGGTTTAGTGTATTACGCCAACGTAATGCTAGAAAAATTATAGTAGCAGGAGCTGCTTCT
ATTAATAAATAGTATAGCAAATATCTCCCTGATTATAGAGTTATTAGTGAAGTTTCAACT
AAAATCAAGGAAGATGGTACTTTAGAGATAGTAGAGCTTTCAGAACAAGAAATAAACAAT
TCAATAACTAATAGCATGAAAGATATTCTTCAAGGATTTTTAAGTTAGTTTGCAACAACT
CACAAAATAAATCCAACCTGGGTTATTATTATTCGTGACGGTATATTTATTTAAAATGCA
CAAAATGAAAAAGATGCTATATTCTCTATGATGAGCACTCGCCGTTCCTAAAGTGAAAAC
AAATCATTCCACCTTTTGTTTGCAACATCTCGTTATCAAACTAGCTTCCGTACTTATAAT
TTCTATGGATCATCAGAACTTAATGCTAACACATAAAACGTATCTTCTCCAGGAACAGGT
GCTATAGTTGAAAAGTAGTTGAACAAGCGCGAATTTTATCTTTATACATAAAAAATAGTT
AAAAACTGTCCATTTTCTGTGCTATACAATGTATTACATATAGATAAATTTATTGGATTA
GACGAGCATTCTTTGTTAGCTGATTTCTAAGACTTGGTATATAACACTTATGTGCTAAAT
TATACATACCACACAAAGACAACTTCACCAGCTTGTGTACAAAATGCTTATAAATTAGCA
GAATTTGTTAAAATGTTTGTAACACCTGCAGGGTCTTAATAAAATACAATAGATCTATCA
AACTTGAGAGTGCTGTGCTCAAATTATAAGCTCTTCTTCATTTGA

>TTHERM_00653810(gene)
ATGGATCAGCAATAGATTTATAAGTTATTTTTGTATTCCAATCTTTTGAAGATGGATATA
CAATCACCCATTTATAAGCAAGATGTTTAGATAACTCCTGAAAAATTTGCTTCTAGTTCA
GATAGAGAAGCTGTATTCTTGGGAGTGCGCAAAATATTACATATCTCTCATGATATGGAC
AATTGTATTTTTTTAAAGAAAAACAATATTCACCTTAGTGAAGAAGTTTAAGAGAATCCA
TAAGCACAATGGGTTTGCTATAGCAGTAAATAAATGTACTAAGGTTAGGATAGTATGAGT
GTTTTAGGATACTAGGTTAGTATTAAATAGATTTAACAAAATGGTAGTCCTGCATTTATA
ACTACCAACAAAGAGAGATAGCTTGTTTTAAATTATATTGCAAGAGAAATTTTTTCAAAA
CTAAATTATCATTAATACTAAAAAGGGTTTTTCAATCCTATTGACCCCCCTAATGCTGCC
CTTAGTAAAGATAAAACTGTTGTTTATCAAACTTACTCATGGAATGGGCTAAGACCTAAG
GCAATTGAATTTAGAGGTAAAGTTTATCTGGAAATTGAAACCACTCCTGTGCGCTTCTTG
CGTATGAATTTGTTCTAAATTATTAACAAACTTGAAGATAAAAAAATTCCAAGAGAAGAA
ATAAGCAATTTTTTGGTAGGACGCACTGTTAAGACATCTTATTTCTCCACTAATCTATAT
TATGAAATTTTAGAAATAGATTTTAATATGACTCCTGACAGTAAATTTGTCGTAAAATAA
AACTAAATTTAAGATGAAGGAGATATGATTGCAGAAAAAGGTGATGAAGAACTCTCTTAT
TATTCTTATGTTAAGAACAAATATAGAATTAACATTAGAGACAGAGTTTAGCCAATGATT
AGAGTTCGCCCTCTTCACAAAGGCTTTAAATCCGAATTTCATAGTAGTTAAGATTAGCAA
ATGTACCTCATTCCAAGTCTTTGCAATCTTTTTGGATAGATTTCTCAAAAAGGTTTTATT
GACGATAATGTTGGAACAGAATTCTACTTTGAACCTTATGATGTATATGATTATACTAAT
TAAATTACTAGATTTTTAAAAAATAGCTCTGAAGCTTAAAGTTTGCTAAATGGATGGTAA
ATTAAAATAGGAGAAGAGTTCGAACAGATTGGTTTTAACTACCTCAAATTATCTCCTTTA
TAACTCAAAGTTAATAATGTCAATGAGTCTTTTTCTCCTAACGACTTATGCTGTAAGCGT
TTATGTAACGAATTCGATGGATATTATAAATAGCTATTCTGTTCTGATGGAGCTTTCACT
AAATTCACTGAAGTTAATAGATGGGTTATTTTTGTCCACTCTAGCGATTCACCATTAGTT
GAAGCTCTGGTCTCTTCGATCTCTCAGTTTAAGCTATACTACAATTACCCTATTTCAGAA
CCAAAATATTATAAGATAGATGATTTAAATATTGAAACATGGACAAGCACCTTTAATACT
GCCTTATCTGAGAGTCCTACTACATTTGCATTGATTATTATGAACAGTGAATTAGAGCAC
TATCACTTTTATAAAACTTGTAAGCAAGCAATTACAGTGGATAAAGGCATTTGCTGTTCA
CTTATTAAGGCTTCTACTCTCTAGCAATATGTTCCTACTTAATAATATCCATAAATACCC
CCAGGAAACTCGATTGAAGCTTTTGCAAGCCGTTTCTTATCATAAATGCAAGCTAAGCTT
GGTGAGTCACCTTGGCTTATGTAGGAATTACCATACAATTAAAATCCTTGCACAATGATA
GGGTTTAGTGTATTACGCCAACGTAATGCTAGAAAAATTATAGTAGCAGGAGCTGCTTCT
ATTAATAAATAGTATAGCAAATATCTCCCTGATTATAGAGTTATTAGTGAAGTTTCAACT
AAAATCAAGGAAGATGGTACTTTAGAGATAGTAGAGCTTTCAGAACAAGAAATAAACAAT
TCAATAACTAATAGCATGAAAGATATTCTTCAAGGATTTTTAAGTTAGTTTGCAACAACT
CACAAAATAAATCCAACCTGGGTTATTATTATTCGTGACGGTATATTTATTTAAAATGCA
CAAAATGAAAAAGATGCTATATTCTCTATGATGAGCACTCGCCGTTCCTAAAGTGAAAAC
AAATCATTCCACCTTTTGTTTGCAACATCTCGTTATCAAACTAGCTTCCGTACTTATAAT
TTCTATGGATCATCAGAACTTAATGCTAACACATAAAACGTATCTTCTCCAGGAACAGGT
GCTATAGTTGAAAAGTAGTTGAACAAGCGCGAATTTTATCTTTATACATAAAAAATAGTT
AAAAACTGTCCATTTTCTGTGCTATACAATGTATTACATATAGATAAATTTATTGGATTA
GACGAGCATTCTTTGTTAGCTGATTTCTAAGACTTGGTATATAACACTTATGTGCTAAAT
TATACATACCACACAAAGACAACTTCACCAGCTTGTGTACAAAATGCTTATAAATTAGCA
GAATTTGTTAAAATGTTTGTAACACCTGCAGGGTCTTAATAAAATACAATAGATCTATCA
AACTTGAGAGTGCTGTGCTCAAATTATAAGCTCTTCTTCATTTGA

>TTHERM_00653810(protein)
MDQQQIYKLFLYSNLLKMDIQSPIYKQDVQITPEKFASSSDREAVFLGVRKILHISHDMD
NCIFLKKNNIHLSEEVQENPQAQWVCYSSKQMYQGQDSMSVLGYQVSIKQIQQNGSPAFI
TTNKERQLVLNYIAREIFSKLNYHQYQKGFFNPIDPPNAALSKDKTVVYQTYSWNGLRPK
AIEFRGKVYLEIETTPVRFLRMNLFQIINKLEDKKIPREEISNFLVGRTVKTSYFSTNLY
YEILEIDFNMTPDSKFVVKQNQIQDEGDMIAEKGDEELSYYSYVKNKYRINIRDRVQPMI
RVRPLHKGFKSEFHSSQDQQMYLIPSLCNLFGQISQKGFIDDNVGTEFYFEPYDVYDYTN
QITRFLKNSSEAQSLLNGWQIKIGEEFEQIGFNYLKLSPLQLKVNNVNESFSPNDLCCKR
LCNEFDGYYKQLFCSDGAFTKFTEVNRWVIFVHSSDSPLVEALVSSISQFKLYYNYPISE
PKYYKIDDLNIETWTSTFNTALSESPTTFALIIMNSELEHYHFYKTCKQAITVDKGICCS
LIKASTLQQYVPTQQYPQIPPGNSIEAFASRFLSQMQAKLGESPWLMQELPYNQNPCTMI
GFSVLRQRNARKIIVAGAASINKQYSKYLPDYRVISEVSTKIKEDGTLEIVELSEQEINN
SITNSMKDILQGFLSQFATTHKINPTWVIIIRDGIFIQNAQNEKDAIFSMMSTRRSQSEN
KSFHLLFATSRYQTSFRTYNFYGSSELNANTQNVSSPGTGAIVEKQLNKREFYLYTQKIV
KNCPFSVLYNVLHIDKFIGLDEHSLLADFQDLVYNTYVLNYTYHTKTTSPACVQNAYKLA
EFVKMFVTPAGSQQNTIDLSNLRVLCSNYKLFFI