Identifiers and Description

Gene Model Identifier

TTHERM_00881440

Standard Name

CYP1 (CYsteine Protease 1 )

Aliases

CyP | PreTt09744 | 156.m00128

Description

CYP1 papain family cysteine protease; Putative cysteine protease; mRNA is specifically expressed during starvation; histone H1 and Hhp1p are required for efficient mRNA expression; Peptidase C1A

Genome Browser (Macronucleus)

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Genome Browser (Micronucleus)

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External Links

Gene Ontology Annotations

Molecular Function

Biological Process

Domains

  • ( PF00112 ) Papain family cysteine protease
  • ( PF08246 ) Cathepsin propeptide inhibitor domain (I29)

Gene Expression Profile

Vegetative Cell Cycle (Zhang et al., 2023)

GeneMania

Tetrahymena Stock Center

No Data fetched for Tetrahymena Stock Center

Homologs

No Data fetched for Homologs

General Information

No. Gene Name(s) Paragraph Text
2216 NGOA1, HHO1, CYP1, CDC2 HHO1 knockouts show no global increase or decrease in the amount of transcription in the cell; however, these same knockouts also show that Hho1p is important for the transcriptional regulation of individual genes in response to stimuli, such as starvation. The differential regulation of Hho1p by phosphorylation under vegetative growth and starvation conditions has been well studied. During vegetative growth, Hho1p is phosphorylated on five closely spaced residues, preventing it from interacting with chromatin, likely by interfering with its ability to bind DNA. Under these conditions, expression is increased for CDC2, a homolog of the cyclin dependent kinases responsible for histone H1 phosphorylation, possibly creating a positive feedback loop that promotes the cell cycle. During starvation conditions, Hho1p is dephosphorylated, allowing it to bind to chromatin. This stimulates the expression of some genes, including ngoA, and protease genes such as CYP1, while inhibiting expression of other genes, such as CDC2. This decrease in CDC2 expression may be responsible for cell cycle arrest during starvation.

Associated Literature

  1. Ref:12356861: Dou Y, Bowen J, Liu Y, Gorovsky MA (2002) Phosphorylation and an ATP-dependent process increase the dynamic exchange of H1 in chromatin. The Journal of cell biology 158(7):1161-70
  2. Ref:12351190: Udani RA, Karrer KM (2002) ASI1, a gene encoding a novel leucine zipper protein, is induced during development of the macronucleus in Tetrahymena. Mechanisms of development 118(1-2):215-8
  3. Ref:10983971: Dou Y, Gorovsky MA (2000) Phosphorylation of linker histone H1 regulates gene expression in vivo by creating a charge patch. Molecular cell 6(2):225-31
  4. Ref:10207086: Huang H, Smothers JF, Wiley EA, Allis CD (1999) A nonessential HP1-like protein affects starvation-induced assembly of condensed chromatin and gene expression in macronuclei of Tetrahymena thermophila. Molecular and cellular biology 19(5):3624-34
  5. Ref:9163512: Suzuki KM, Hosoya N, Takahashi T, Kosaka T, Hosoya H (1997) Release of a newly-identified cysteine protease, tetrain, from Tetrahymena into culture medium during the cell growth. Journal of biochemistry 121(4):642-7
  6. Ref:8756729: Shen X, Gorovsky MA (1996) Linker histone H1 regulates specific gene expression but not global transcription in vivo. Cell 86(3):475-83
  7. Ref:8464925: Karrer KM, Peiffer SL, DiTomas ME (1993) Two distinct gene subfamilies within the family of cysteine protease genes. Proceedings of the National Academy of Sciences of the United States of America 90(7):3063-7

Sequences 

>TTHERM_00881440(coding)
ATGAATAAAAAATTCATCATTTTGAGTATTATCATGCTCATGCCTCTCTGTTTGGCTCAA
GATATAAGTGTAGAAAAACTTCTTGCTTATAATAAATGGTCAAGCTAAAATCAAAGAGCC
TATCTGAATGAAGATGAAAAACTGTATAGACAAATAGTTTTCTTTGAAAATTTGTAAAAA
ATTAAGGAGCATAACAGTAACCCTAATAACACCTATTCTATCCATTTAAACTAATTCTCA
GATATGACTAGAGAAGAATTTGCAGAAAAAATTCTTATGAAATAGGATTTGATTAACGAT
TATATGAAGGGAATTGGTTAATAGGCTACTCACAATAATGCTAATAACGAAACTTAAATG
AATTCATAAAACCATACTTTAGCTGCTTCTATAGATTGGAGAACAAAAGGTGCTGTAACA
TCGGTTAAGGATTAAGGTTAATGTGGTTCATGCTGGAGTTTCTCTGCAGCTGCCTTAATG
GAGTCATTTAACTTCATTTAAAACAAAGCTTTAGTTAATTTTTCTGAGTAATAACTTGTT
GATTGTGTGACCCCTGAAAATGGTTACCCCTCTTATGGATGTAAAGGAGGATGGCCTGCT
ACTTGTCTGGATTATGCCTCCAAAGTAGGTATCACAACACTAGACAAGTATCCCTATGTT
GCAGTACAGAAAAATTGTACTGTGACAGGTACAAATAATGGCTTTAAGCTTAAAAAGTGG
ATTGTAATTCCTAACACTTCAAACGACTTAAAAAGTGCTTTAAATTTCTCTCCTGTTTCT
GTTCTTGTTGATGCTACCAATTGGGATTATTATTCGTCTGGAATTTTCAACGGATGTAAT
TAAACTAATATTAATCTTAATCATGCTGTATTAGCTGTAGGCTATGACGAAAAAGATAAC
TGGATTGTTAAAAATTCTTGGAGCGCTGGTTGGGGTGAACATGGATATATTAGACTTGCT
CCTAACAATACATGTGGTATCTTAAGCTCTAATATATAAGTTACTGCTTGA



>TTHERM_00881440(gene)
TCACTTTCCAAATTGAAACTCGCCATACTTTATATACTTAGAAATCAATACTCAAAACAA
TAAACAACAAATAAAAAAAAATAAAAAAACTAAAACTTTAAAGTATGAATAAAAAATTCA
TCATTTTGAGTATTATCATGCTCATGCCTCTCTGTTTGGCTCAAGATATAAGTGTAGAAA
AACTTCTTGCTTATAATAAATGGTCAAGCTAAAATCAAAGAGCCTATCTGAATGAAGATG
AAAAACTGTATAGACAAATAGTTTTCTTTGAAAATTTGTAAAAAATTAAGGAGCATAACA
GTAACCCTAATAACACCTATTCTATCCATTTAAACTAATTCTCAGATATGACTAGAGAAG
AATTTGCAGAAAAAATTCTTATGAAATAGGATTTGATTAACGATTATATGAAGGGAATTG
GTTAATAGGCTACTCACAATAATGCTAATAACGAAACTTAAATGAATTCATAAAACCATA
CTTTAGCTGCTTCTATAGATTGGAGAACAAAAGGTGCTGTAACATCGGTTAAGGATTAAG
GTTAATGTGGTTCATGCTGGAGTTTCTCTGCAGCTGCCTTAATGGAGTCATTTAACTTCA
TTTAAAACAAAGCTTTAGTTAATTTTTCTGAGTAATAACTTGTTGATTGTGTGACCCCTG
AAAATGGTTACCCCTCTTATGGATGTAAAGGAGGATGGCCTGCTACTTGTCTGGATTATG
CCTCCAAAGTAGGTATCACAACACTAGACAAGTATCCCTATGTTGCAGTACAGAAAAATT
GTACTGTGACAGGTACAAATAATGGCTTTAAGCTTAAAAAGTGGATTGTAATTCCTAACA
CTTCAAACGACTTAAAAAGTGCTTTAAATTTCTCTCCTGTTTCTGTTCTTGTTGATGCTA
CCAATTGGGATTATTATTCGTCTGGAATTTTCAACGGATGTAATTAAACTAATATTAATC
TTAATCATGCTGTATTAGCTGTAGGCTATGACGAAAAAGATAACTGGATTGTTAAAAATT
CTTGGAGCGCTGGTTGGGGTGAACATGGATATATTAGACTTGCTCCTAACAATACATGTG
GTATCTTAAGCTCTAATATATAAGTTACTGCTTGAAAATTAGGATAAGCTATTATAATTA
AAATTTATTAAAATATATTATTCGTATAAACAAAAATATTATATAAAATAAATAGTCTTT
CAAATATTAAGATTTGTTTAATTTTAAAAAATAAAAACTTATTTATTTTAACATTAAATT
AATATTAAAAAATAATAATTTATATTTAAAAATAGCTTGACAAAATAAGTTAAAGTAAAT
TGATTTTATAAAAAATTCAAATAAATTACTACAAAATTATTTAATATAAACTTATTAATT
ATAAATATACTGCATAGTTAATCAAAGCTTCTTTATATTAAGACCTCTTACTAAAATTTT
CTGGACATACAAAAAATTTAAAAAAAAAATCTTGTAAAAAGGGCCACTAGACAGACTTCG
CTTTTCCTATTATATTTAATTCCCAAATTAAAGCTAAAAATAAAATTATTAATAATAAAT
AATTTTCATAAAAACTTTCTGAAAGCACTCAAGAATAAGCTGTTAAAAATTAATTAATAA
ATTAATACTCTTCCTATTTAAAATATTTTTATAT



>TTHERM_00881440(protein)
MNKKFIILSIIMLMPLCLAQDISVEKLLAYNKWSSQNQRAYLNEDEKLYRQIVFFENLQK
IKEHNSNPNNTYSIHLNQFSDMTREEFAEKILMKQDLINDYMKGIGQQATHNNANNETQM
NSQNHTLAASIDWRTKGAVTSVKDQGQCGSCWSFSAAALMESFNFIQNKALVNFSEQQLV
DCVTPENGYPSYGCKGGWPATCLDYASKVGITTLDKYPYVAVQKNCTVTGTNNGFKLKKW
IVIPNTSNDLKSALNFSPVSVLVDATNWDYYSSGIFNGCNQTNINLNHAVLAVGYDEKDN
WIVKNSWSAGWGEHGYIRLAPNNTCGILSSNIQVTA