Identifiers and Description

Gene Model Identifier

TTHERM_01276320

Standard Name

GIW1 (Gentleman-In-Waiting 1)

Aliases

PreTt28295 | 304.m00015 | COI1

Description

GIW1 hypothetical protein; Twi1p-associated protein

Genome Browser (Macronucleus)

Full Screen View

No Genome Browser Data Present

Genome Browser (Micronucleus)

Full Screen View

No Genome Browser Data Present

Gene Ontology Annotations

Molecular Function

• lysozyme activity (IMP) | GO:0003796 | Ref:20211138
• sequence-specific DNA binding (IDA) | GO:0043565 | Ref:20211138

Biological Process

• cocaine biosynthetic process (IDA) | GO:0050799 | Ref:20211138
• garland nephrocyte differentiation (IMP) | GO:0061321 | Ref:20211138
• lipoate metabolic process (IDA) | GO:0009106 | Ref:20211138
• regulation of steroid biosynthetic process (IMP) | GO:0050810 | Ref:20211138

Domains

• ( PF01114 ) Colipase, N-terminal domain
• ( PF01846 ) FF domain
• ( PF02071 ) Aromatic-di-Alanine (AdAR) repeat
• ( PF08158 ) NUC130/3NT domain
• ( TIGR00208 ) flagellar protein FliS

Gene Expression Profile

Tetrahymena Stock Center

• ( SD01818 ) Macronucleus: All GIW1 genes replaced by c-terminal HA tagged version with neo3 in 3’ flanking region
• ( SD01819 ) Macronucleus: All GIW1 genes replaced by c-terminal HA tagged version with neo3 in 3’ flanking region
• ( SD01820 ) Macronucleus: KO of GIW1 with neo3
• ( SD01821 ) Macronucleus: KO of GIW1 with neo3
• ( SD01822 ) Macronucleus: KO of GIW1 with neo3
• ( SD01823 ) Macronucleus: KO of GIW1 with neo3
• ( SD01824 ) Macronucleus: All GIW1 genes replaced by c-terminal V5 tagged version with neo3 in 3' flanking region
• ( SD01825 ) Macronucleus: All GIW1 genes replaced by c-terminal V5tagged version with neo3 in 3’ flanking region
• ( SD01826 ) Macronucleus: All GIW1 genes replaced by c-terminal V5 tagged version with neo3 in 3’ flanking region
• ( SD01827 ) Macronucleus: All GIW1 genes replaced by c-terminal V5 tagged version with neo3 in 3’ flanking region

Homologs

Source	Identifier	Score
Tetrahymena borealis	EI9_21071.1	4.998338612125368e-60
	Description: hypothetical protein (376 aa)

General Information

No.	Gene Name(s)	Paragraph Text
104	GIW1, TWI1	Giw1p binds to Twi1p complexed with single-stranded, but not double-stranded, scnRNAs and that this interaction selectively promotes the MAC localization of the mature Twi1p-scnRNA complex (Noto et al. 2010).
105	GIW1, COI12, TWI1	ATP-dependent and ATP-independent activities of Coi12p facilitate scnRNA loading by counteracting the Twi1p-binding protein Giw1p, which is important for sorting scnRNAs to Twi1p (Woehrer et al. 2015).

Associated Literature

1. Ref:29021213: Noto T, Mochizuki K (2017) Whats, hows and whys of programmed DNA elimination in Tetrahymena. Open biology 7(10):
2. Ref:26688337: Kataoka K, Mochizuki K (2015) Phosphorylation of an HP1-like Protein Regulates Heterochromatin Body Assembly for DNA Elimination. Developmental cell 35(6):775-88
3. Ref:25588944: Woehrer SL, Aronica L, Suhren JH, Busch CJ, Noto T, Mochizuki K (2015) A Tetrahymena Hsp90 co-chaperone promotes siRNA loading by ATP-dependent and ATP-independent mechanisms. The EMBO journal 34(4):559-77
4. Ref:22103557: Mochizuki K (2012) Developmentally programmed, RNA-directed genome rearrangement in Tetrahymena. Development, growth & differentiation 54(1):108-19
5. Ref:21915788: Kataoka K, Mochizuki K (2011) Programmed DNA elimination in Tetrahymena: a small RNA-mediated genome surveillance mechanism. Advances in experimental medicine and biology 722( ):156-73
6. Ref:20211138: Noto T, Kurth HM, Kataoka K, Aronica L, DeSouza LV, Siu KW, Pearlman RE, Gorovsky MA, Mochizuki K (2010) The Tetrahymena argonaute-binding protein Giw1p directs a mature argonaute-siRNA complex to the nucleus. Cell 140(5):692-703

Sequences

>TTHERM_01276320(coding)
ATGTAAAGTTTTGTAAATCTTATCTAACTGGAAGAGAACTATAACTTAAGTCTTTTGTCT
TATTCAAACTCTATAAGGTAGTATCTATTAGATAACAAAGTGCACGAAGATAAATACATA
TAGCTCATGAAAGACATCAAAGATTTGTTTTCGTCTCCTACTTTTTTGATGCCAAATTTC
TCTGACAAAAGAAAATAATTTTTCTCATAATTATGCTTAATTGCTTTTGATTTTGCTAAT
AATTAAGTTACTGAATAATAATCTTTGAGTGAATTTAGAAAAAAATTCAAATTTCCTTTT
GTAATAATTGCTATTATTTACACTAAACTTTTAGAGATAAAAGAAGCCTTACCTGAAGAT
TTCTTAACTCTAGTGGATTGTCACTTCAAAAATAAAGGTCCTTAAAGTATATTTGCCTCT
TTGACATTGATTTCTTTATTTTTGAATGAAGTAGATTAATATGAAATAGATTGTGACCAT
ATTGAATCTTCAAATACATTAAGTAGTTGTAGCGAGGCTGCAGAGTCTAATATTCCTAAA
AAGGTCAATATAACTGTAAAGACAGTCTAAAATTTCTTTGATAAATAAGGATTATAGATG
ATAGCATTTCCCTTAAATGTTATCAAAAATATTCACTGTGTAGCACATGATCAATAGAGT
ATTATCCAGCTATTGCTAAAAATAGTTGTGATAATAATTAAAAACATGAGTAACAAAATC
AAATAAGAGTTTTTTTATTTTCATTAGAAGGATTTAAATGTGATTCTTTCATGTTTTATA
GGCTTTTTGTAGTAAAATAACTAGGCTAGAGAAGATCGCGATGTAGCTTTAGCAAATATT
TTTGAATTTTGCGAATTGCTAGCTAAATCTACTTCACCTGCTTAATTATACGTTGCGTTT
AAAGATTTTTTAGTTTATATTTCATAGAATTTTTCATTTGCTAATGATAGTATAGAATAC
TTTGACTTCAATTCTAAGTGTGTGGATTTTTTAGTTTCTATTACTCCATGCTTTATTTAA
TAGTCTGAGAATTTATCTGCATATCCTTTAATAATATAGTTATTGAAGTAATCTGCTAAA
ATAAATCTTGTGAATTTAGAGGATTAGATAGGCTGCTTGCATATATGGAGCCATTATTCT
GACTAGTTCATTCAGGCTTACGATAATAATAATAATTCAATAAAAGCTGAAACAATTAAT
AACTTAGCTATTCTGAATTCTACTTTTATTGAATCTCTACAATTTGATATAGAGCTATTA
TTTAATTTGTAGATAAAAGATCATGATTCTGAAGATGTTAATTAATTTGTGGACCAACTA
TCCCGCTTAGTTATTCATTCTAATCTCATTGATAACCAATTATTTGTAGAAGTAATAAAA
TCTTTGAGTCTACTTTATTAGAAAAATATGCTCCTTTATTATACTTTCCAAAAATGTTTG
ATGAAGTAGTACCTTAATTCTAAAGATACTCTCAGCTAAATTTACTAAGTTTTTGGCAAG
AGTAATGATTTTCTTTAGATTACAGAATTGTTATTTGAATCTTTTAGTTAAGCACAAAAA
TAATTTGCAAATTTAGATCTAAATACCATAGCTGCTATTGAAAAATTTACATAGTTCATG
TCTTAAATAGGTGCAAAGCTGTTTAATACTTAAATATCTCATCTTGAGTAGTCTTAAATT
TTGGGAATATTTCAAAAATATATTTTGAACCCAATTAAAATGTTTAATTTAAATATATTT
TCTTAATTACCTGAAAATTTTATACAGCTTAATGAAGTTTAATTTAGTCAAGTCTGCACA
ATGATGGAGTCAGTTCATTACTTTATTTTCTCTGACAATAAAAGCAATTCAGATTAAGTT
TTTGAGACAGAGGTTGATGTTTCTATGATAAAAAAGATATTGTTATTCAATTAATCAGTT
AACTAAGACATGGCCTACTGGATTAACCCACAATTAAAGAATGAAAGAAAAAAAATAAAA
TAATTCTACCAAAGTACAATTTATTGGGTAATATTTGCTTCAATTAATCTTGAAAATGCT
GCTAACATATTTGAAAGCTATTTCTTTTAGAAAGAGTTCAATATTTGGGTATTGAATTCT
ACTATCAAAAAGCTCAAAGGTAGGATATTACCAAACACATTTCTGCTTTACTTGTGTTAC
TATTTAAAATCCCTCACTGACTCTGATAGTTTTAGCAAAATTCCTAAGTAAGAGCTATTA
GAAAACTCTCAGATCTATAAAACTATTTCTCTTTACTTAAATCTGGCAATTAGATTTAAA
ATGGATTTTCTTGAGAAAGAAGAAACTTAGTCAGAAAATTCTCACTATTTTAATTTCATT
CTAGAGATATTAGACCACATGTATTTTACTAATAAAAAAATTGTGCTTGATTTTTGTTAA
AAGATATTGAATTATTTTGATTAAGATTGCTATAATTAAAACATTATTTAATTAGCTTAA
TCTAGCCAAACTTTTTAAAACATCACTCTTTAGCTTTTTACTAATATTTGGGAGTAAATC
TTCAATTTTTGTTCTAGTAGCAATGAAAAGGCAGAAGAGTTTAAAGAAAAAGATATTAGC
TAATCTATTGATTTTATTTTAAAATTCTCTAAGTGTTTATAAGGTAACTTGTCAATAATT
TAAGACCGTATTATGCTTGTTATAAAAGCCCATCCTATTTATAGTTCGAATTCTCAAATT
ATTTATTCCACTATCATAGAAAATTTGGTAATTAAAGAAATAGATGGAGTTAAGGTTCTT
TAAAATGTTTTTTTAAAATCTATAATTAAAGACTTACAGATTTGGGCTAAATCTATATGT
CCTTAGAGATTACAAAGCTGA

>TTHERM_01276320(gene)
ATGTAAAGTTTTGTAAATCTTATCTAACTGGAAGAGAACTATAACTTAAGTCTTTTGTCT
TATTCAAACTCTATAAGGTAGTATCTATTAGATAACAAAGTGCACGAAGATAAATACATA
TAGCTCATGAAAGACATCAAAGATTTGTTTTCGTCTCCTACTTTTTTGATGCCAAATTTC
TCTGACAAAAGAAAATAATTTTTCTCATAATTATGCTTAATTGCTTTTGATTTTGCTAAT
AATTAAGTTACTGAATAATAATCTTTGAGTGAATTTAGAAAAAAATTCAAATTTCCTTTT
GTAATAATTGCTATTATTTACACTAAACTTTTAGAGATAAAAGAAGCCTTACCTGAAGAT
TTCTTAACTCTAGTGGATTGTCACTTCAAAAATAAAGGTCCTTAAAGTATATTTGCCTCT
TTGACATTGATTTCTTTATTTTTGAATGAAGTAGATTAATATGAAATAGATTGTGACCAT
ATTGAATCTTCAAATACATTAAGTAGTTGTAGCGAGGCTGCAGAGTCTAATATTCCTAAA
AAGGTCAATATAACTGTAAAGACAGTCTAAAATTTCTTTGATAAATAAGGATTATAGATG
ATAGCATTTCCCTTAAATGTTATCAAAAATATTCACTGTGTAGCACATGATCAATAGAGT
ATTATCCAGCTATTGCTAAAAATAGTTGTGATAATAATTAAAAACATGAGTAACAGTATA
GATTTCACTTAAATTTACTTTTAAAATATATTAAATTATTTTTTTAATTAATTTAGAAAT
CAAATAAGAGTTTTTTTATTTTCATTAGAAGGATTTAAATGTGATTCTTTCATGTTTTAT
AGGCTTTTTGTAGTAAAATAACTAGGCTAGAGAAGATCGCGATGTAGCTTTAGCAAATAT
TTTTGAATTTTGCGAATTGCTAGCTAAATCTACTTCACCTGCTTAATTATACGTTGCGTT
TAAAGATTTTTTAGTTTATATTTCATAGAATTTTTCATTTGCTAATGATAGTATAGAATA
CTTTGACTTCAATTCTAAGTGTGTGGATTTTTTAGTTTCTATTACTCCATGCTTTATTTA
ATAGTCTGAGAATTTATCTGCATATCCTTTAATAATATAGTTATTGAAGTAATCTGCTAA
AATAAATCTTGTGAATTTAGAGGATTAGATAGGCTGCTTGCATATATGGAGCCATTATTC
TGACTAGTTCATTCAGGCTTACGATAATAATAATAATTCAATAAAAGCTGAAACAATTAA
TAACTTAGCTATTCTGAATTCTACTTTTATTGAATCTCTACAATTTGATATAGAGCTATT
ATTTAATTTGTAGATAAAAGATCATGATTCTGAAGATGTTAATTAATTTGTGGACCAACT
ATCCCGCTTAGTTATTCATTCTAATCTCATTGATAACCAATTGTAATTTTTTAGTTTACT
TTAAAATATAGTAATGTTTAATTATCTTTTTTGTTTATTTATAAAAATATTAATTAGATT
TGTAGAAGTAATAAAATCTTTGAGTCTACTTTATTAGAAAAATATGCTCCTTTATTATAC
TTTCCAAAAATGTTTGATGAAGTAGTACCTTAATTCTAAAGATACTCTCAGCTAAATTTA
CTAAGTTTTTGGCAAGAGTAATGATTTTCTTTAGATTACAGAATTGTTATTTGAATCTTT
TAGTTAAGCACAAAAATAATTTGCAAATTTAGATCTAAATACCATAGCTGCTATTGAAAA
ATTTACATAGTTCATGTCTTAAATAGGTGCAAAGCTGTTTAATACTTAAATATCTCATCT
TGAGTAGTCTTAAATTTTGGGAATATTTCAAAAATATATTTTGAACCCAATTAAAATGTT
TAATTTAAATATATTTTCTTAATTACCTGAAAATTTTATACAGCTTAATGAAGTTTAATT
TAGTCAAGTCTGCACAATGATGGAGTCAGTTCATTACTTTATTTTCTCTGACAATAAAAG
CAATTCAGATTAAGTAATAACTTTTAAATTAAAAATACTTTTAAAAATTCCTTTTTAAAT
AAAATTTAAAGGTTTTTGAGACAGAGGTTGATGTTTCTATGATAAAAAAGATATTGTTAT
TCAATTAATCAGTTAACTAAGACATGGCCTACTGGATTAACCCACAATTAAAGAATGAAA
GAAAAAAAATAAAATAATTCTACCAAAGTACAATTTATTGGGTAATATTTGCTTCAATTA
ATCTTGAAAATGCTGCTAACATATTTGAAAGCTATTTCTTTTAGAAAGAGTTCAATATTT
GGGTATTGAATTCTACTATCAAAAAGCTCAAAGGTAGGATATTACCAAACACATTTCTGC
TTTACTTGTGTTACTATTTAAAATCCCTCACTGACTCTGATAGTTTTAGCAAAAGTAAAT
TAATTTTATTCATTTATTTATTTCACAAATTTTTTAGTTGTTTAAAAGAGTGAATATGAA
GAAAAATTTAGAAAATTAATGGTAAATATTTCTAAATCTGTACCTTCTGAGATATATGTA
GATTACTCTAAAATTTAGTACTATCTTTAATTATACATTAATGAAGAGTGTTAAATGTTT
AGATTTTAAATATTAAGTGACTAGACAGTCAGCTTACTGACATTAGAAAACAGATTTTCT
CATGTTTATAAAGTATTTATAATATTAGAAGATCTCTTTACAATTGTTTAGAGATTTCCT
AAGTTTTATTATGAAAATACATTTATGGGTCTAAAAAGGAATTTCTTTTTAAATCGTGTT
TTTTGGATTATTGTTAAATGCTTTACAATAGTTCCTAAGTAAGAGCTATTAGAAAACTCT
CAGATCTATAAAACTATTTCTCTTTACTTAAATCTGGCAATTAGATTTAAAATGGATTTT
CTTGAGAAAGAAGAAACTTAGTCAGAAAATTCTCACTATTTTAATTTCATTCTAGAGGTA
TTTAATTTATTTTAATCTAAATTATTTTTATTTAAATTTATTTTTTAAAAAGATATTAGA
CCACATGTATTTTACTAATAAAAAAATTGTGCTTGATTTTTGTTAAAAGATATTGAATTA
TTTTGATTAAGATTGCTATAATTAAAACATTATTTAATTAGCTTAATCTAGCCAAACTTT
TTAAAACATCACTCTTTAGCTTTTTACTAATATTTGGGAGTAAATCTTCAATTTTTGTTC
TAGTAGCAATGAAAAGGCAGAAGAGTTTAAAGAAAAAGATATTAGCTAATCTATTGATTT
TATTTTAAAATTCTCTAAGTGTTTATAAGGTAACTTGTCAATAATTTAAGACCGTATTAT
GCTTGTTATAAAAGCCCATCCTATTTATAGTTCGAATTCTCAAATTATTTATTCCACTAT
CATAGAAAATTTGGTAATTAAAGAAATAGATGGAGTTAAGGTTCTTTAAAATGTTTTTTT
AAAATCTATAATTAAAGACTTACAGATTTGGGCTAAATCTATATGTCCTTAGAGATTACA
AAGCTGA

>TTHERM_01276320(protein)
MQSFVNLIQLEENYNLSLLSYSNSIRQYLLDNKVHEDKYIQLMKDIKDLFSSPTFLMPNF
SDKRKQFFSQLCLIAFDFANNQVTEQQSLSEFRKKFKFPFVIIAIIYTKLLEIKEALPED
FLTLVDCHFKNKGPQSIFASLTLISLFLNEVDQYEIDCDHIESSNTLSSCSEAAESNIPK
KVNITVKTVQNFFDKQGLQMIAFPLNVIKNIHCVAHDQQSIIQLLLKIVVIIIKNMSNKI
KQEFFYFHQKDLNVILSCFIGFLQQNNQAREDRDVALANIFEFCELLAKSTSPAQLYVAF
KDFLVYISQNFSFANDSIEYFDFNSKCVDFLVSITPCFIQQSENLSAYPLIIQLLKQSAK
INLVNLEDQIGCLHIWSHYSDQFIQAYDNNNNSIKAETINNLAILNSTFIESLQFDIELL
FNLQIKDHDSEDVNQFVDQLSRLVIHSNLIDNQLFVEVIKSLSLLYQKNMLLYYTFQKCL
MKQYLNSKDTLSQIYQVFGKSNDFLQITELLFESFSQAQKQFANLDLNTIAAIEKFTQFM
SQIGAKLFNTQISHLEQSQILGIFQKYILNPIKMFNLNIFSQLPENFIQLNEVQFSQVCT
MMESVHYFIFSDNKSNSDQVFETEVDVSMIKKILLFNQSVNQDMAYWINPQLKNERKKIK
QFYQSTIYWVIFASINLENAANIFESYFFQKEFNIWVLNSTIKKLKGRILPNTFLLYLCY
YLKSLTDSDSFSKIPKQELLENSQIYKTISLYLNLAIRFKMDFLEKEETQSENSHYFNFI
LEILDHMYFTNKKIVLDFCQKILNYFDQDCYNQNIIQLAQSSQTFQNITLQLFTNIWEQI
FNFCSSSNEKAEEFKEKDISQSIDFILKFSKCLQGNLSIIQDRIMLVIKAHPIYSSNSQI
IYSTIIENLVIKEIDGVKVLQNVFLKSIIKDLQIWAKSICPQRLQS