Identifiers and Description

Gene Model Identifier

TTHERM_00049080

Standard Name

POB3 (POl1 Binding)

Aliases

PreTt11287 | 3.m01985 | 3824.m03045 | Ssrp1

Description

POB3 Also known as Structure-specific recognition protein (Ssrp1); Interacts with Spt16 (Cet1) as a component of the FACT-complex (Kanwal Ashraf et al., 2019); RTT106/SSRP1 Histone Chaperone/FACT Complex

Genome Browser (Macronucleus)

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Genome Browser (Micronucleus)

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External Links

Gene Ontology Annotations

No Data fetched for Gene Ontology Annotations

Domains

  • ( PF03531 ) Structure-specific recognition protein (SSRP1)
  • ( PF08512 ) Histone chaperone Rttp106-like, middle domain
  • ( PF21103 ) SSRP1 PH domain

Gene Expression Profile

Vegetative Cell Cycle (Zhang et al., 2023)

GeneMania

Tetrahymena Stock Center

No Data fetched for Tetrahymena Stock Center

Homologs

No Data fetched for Homologs

General Information

No. Gene Name(s) Paragraph Text
2335 POB3 The FACT-complex is a critical transcription regulator and an H2A/H2B chaperone. Affinity purification followed by mass spectrometry (AP-MS) of endogenously tagged T. thermophila histones H2A/H2B identified Cet1 and Pob3 subunits of the FACT complex as high-confidence interacting partners. Indirect immunofluorescence studies using endogenously tagged Cet1 indicated that it localized to both the MAC and MIC during vegetative growth. AP-MS analysis using Tetrahymena Cet1 as a bait protein revealed Pob3 and subunits of RNA polymerase as high-confidence co-purifying proteins (Ashraf et al. 2019).

Associated Literature

  1. Ref:30796450: Ashraf K, Nabeel-Shah S, Garg J, Saettone A, Derynck J, Gingras AC, Lambert JP, Pearlman RE, Fillingham J (2019) Proteomic analysis of histones H2A/H2B and variant Hv1 in Tetrahymena thermophila reveals an ancient network of chaperones. Molecular biology and evolution ( ):

Sequences 

>TTHERM_00049080(coding)
ATGAGTGATAGGTGTTATAAAGGTGTCAACTGGGGTAATGTTACCTTTGAAGATGACTAA
TTAGTCCTTAGTCATAACAAGAGAAGATTGTGCAAACTTTCTTTAAAGAAATTCACAAAC
TCTACTGTTAACAAAACTGATATTGTAATTGATTTGAACACAGTTGATCTTCAAGATGAT
GAAGACTAACTCTGTGAAATGAGATTATTTATTCCTCAATAATAAGACGCTTAAATGAAA
CCTGAAGATGATGGAGAAGGTGAAGAAAAAGAAGGTGAAGATAGCGTAGGTGGATATGCA
GATTAACTTAACTCTGAAATTATTACTAAAGCTAAAATTGGCTAATACTCTGGTTAATCA
ATAGTTAAATTCGAAGACATATCACTTCTTGTTCCTAGAGGTAAATACCAACTTGATATG
TACAAAAAAACAGTAAGATTCCACGGCTCTTCTTTTAACTATATAGTAGAATATAGTAAT
GTTATTAAGGGCTTCCTCCTTCCTCAACCTGACGAAGTACATGTTGCTTTCGTTCTTGGT
TTAGATTAACCCTTAAAAATTGGTAATACTGTTCACTCTTACATAGTTATGCAATTCAAA
AAAGAACAAAAGGCTAACATCAAAGTAAACATTGATCCTGAAGAAAAGAAGGAAGATAAA
TTAAAGGATCTCGATGAAGAATATGATGGATTCTTATATGAAATTGCTGGTTAATTGTTT
AAAACTTTATGTAATAATGTATAAATCATCATGCCTGCTGGTTTCTAAAGCTCTGATAAG
TAAAATTGCTTGAAATGTACCCTTAAAACTCACCAAGGTCTTTTATATCCTATGAGAAAG
AGTTTGATCTTTATATATAAACCTGTAATTCACATATAAATCAGTGATATTTAAAAGGTC
GAATTTAATAGAGTTGGTAATGCTACTCTGAATAAATTGTTTGATGTCAAAGTATTTACT
AAGACCACAACCCCTCAATTCTTTGGTTTTGAAAGAAAGGAATTAGATGTCCTCTTAGAA
TACTTTAAATCTAAAAATATTAAGATTACATATGATGATACTAATTAAGGAGCCACTTTT
GATGATGAGGATGAGGAATTCACAGACTCTATTTCTGAAGATGAGGAAGAAGGTAAGAGA
GCTCAAAGAAAGAGTGCTAAGAGAGCTAATAAAGCAGTAAAAGGATAATCAGGATTACCC
CTTGACGATGATGACGAAGAAGAAGACGAAGACTTTGATGCTGATGAATTTGAAAGTGAA
GATGATGAGGAAGAGGAAGAAGACGAAGATGAAGATGAAGATTTTGAATAATGA



>TTHERM_00049080(gene)
ACAATAATAAAGATATTTAATTAATAATAACTTAGATAAATATTTATAATTATTAAATTT
AACAATAAATTATTCATATCTCTACAATTCAAGAATATTTCTTCAAATTTTAAATGAGTG
ATAGGTGTTATAAAGGTGTCAACTGGGGTAATGTTACCTTTGAAGATGACTAATTAGTCC
TTAGTCATAACAAGAGAAGATTGTGCAAACTTTCTTTAAAGAAATTCACAAACTCTACTG
TTAACAAAACTGATATTGTAATTGATTTGAACACAGTTGATCTTCAAGATGATGAAGACT
AACTCTGTGAAATGAGATTATTTATTCCTCAATAATAAGACGCTTAAATGAAACCTGAAG
ATGATGGAGAAGGTGAAGAAAAAGAAGGTGAAGATAGCGTAGGTGGATATGCAGATTAAC
TTAACTCTGAAATTATTACTAAAGCTAAAATTGGCTAATACTCTGGTTAATCAATAGTTA
AATTCGAAGACATATCACTTCTTGTTCCTAGAGGTAAATACCAACTTGATATGTACAAAA
AAACAGTAAGATTCCACGGCTCTTCTTTTAACTATATAGTAGAATATAGTAATGTTATTA
AGGGCTTCCTCCTTCCTCAACCTGACGAAGTACATGTTGCTTTCGTTCTTGGTTTAGATT
AACCCTTAAAAATTGGTAATACTGTTCACTCTTACATAGTTATGCAATTCAAAAAAGAAC
AAAAGGCTAACATCAAAGTAAACATTGATCCTGAAGAAAAGAAGGAAGATAAATTAAAGG
ATCTCGATGAAGAATATGATGGATTCTTATATGAAATTGCTGGTTAATTGTTTAAAACTT
TATGTAATAATGTATAAATCATCATGCCTGCTGGTTTCTAAAGCTCTGATAAGTAAAATT
GCTTGAAATGTACCCTTAAAACTCACCAAGGTCTTTTATATCCTATGAGAAAGAGTTTGA
TCTTTATATATAAACCTGTAATTCACATATAAATCAGTGATATTTAAAAGGTCGAATTTA
ATAGAGTTGGTAATGCTACTCTGAATAAATTGTTTGATGTCAAAGTATTTACTAAGACCA
CAACCCCTCAATTCTTTGGTTTTGAAAGAAAGGAATTAGATGTCCTCTTAGAATACTTTA
AATCTAAAAATATTAAGATTACATATGATGATACTAATTAAGGAGCCACTTTTGATGATG
AGGATGAGGAATTCACAGACTCTATTTCTGAAGATGAGGAAGAAGGTAAGAGAGCTCAAA
GAAAGAGTGCTAAGAGAGCTAATAAAGCAGTAAAAGGATAATCAGGATTACCCCTTGACG
ATGATGACGAAGAAGAAGACGAAGACTTTGATGCTGATGAATTTGAAAGTGAAGATGATG
AGGAAGAGGAAGAAGACGAAGATGAAGATGAAGATTTTGAATAATGATAAAATTGGATTA
AAAAATTAACTTAGTATTGTAATTCCATAAAAATAAATAAATAAATGTATATATAATTTT
CTTTTATCATAAGATTCTGCTAAATTTTATAATTAAAAATTAA



>TTHERM_00049080(protein)
MSDRCYKGVNWGNVTFEDDQLVLSHNKRRLCKLSLKKFTNSTVNKTDIVIDLNTVDLQDD
EDQLCEMRLFIPQQQDAQMKPEDDGEGEEKEGEDSVGGYADQLNSEIITKAKIGQYSGQS
IVKFEDISLLVPRGKYQLDMYKKTVRFHGSSFNYIVEYSNVIKGFLLPQPDEVHVAFVLG
LDQPLKIGNTVHSYIVMQFKKEQKANIKVNIDPEEKKEDKLKDLDEEYDGFLYEIAGQLF
KTLCNNVQIIMPAGFQSSDKQNCLKCTLKTHQGLLYPMRKSLIFIYKPVIHIQISDIQKV
EFNRVGNATLNKLFDVKVFTKTTTPQFFGFERKELDVLLEYFKSKNIKITYDDTNQGATF
DDEDEEFTDSISEDEEEGKRAQRKSAKRANKAVKGQSGLPLDDDDEEEDEDFDADEFESE
DDEEEEEDEDEDEDFEQ