Identifiers and Description

Gene Model Identifier

TTHERM_00049080

Standard Name

POB3 (POl1 Binding)

Aliases

PreTt11287 | 3.m01985 | 3824.m03045 | Ssrp1

Description

POB3; Also known as Structure-specific recognition protein (Ssrp1). Interacts with Spt16 (Cet1) as a component of the FACT-complex (Kanwal Ashraf et al., 2019).

Genome Browser (Macronucleus)

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Genome Browser (Micronucleus)

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External Links

Gene Ontology Annotations

Cellular Component

Molecular Function

Domains

No Data fetched for Domains

Gene Expression Profile

Vegetative Cell Cycle (Zhang et al., 2023)

GeneMania

Tetrahymena Stock Center

No Data fetched for Tetrahymena Stock Center

Homologs

Source Identifier Score
Tetrahymena borealis EI9_01649.1 9.997093137033517e-262
Description: structure-specific recognition protein (437 aa)
Oxytricha Contig22582.0.g104 6.001629673589058e-65
Description: Structure-specific recognition protein (SSRP1)
SGD YML069W 2.999948199478294e-54
Description: POB3 Subunit of the heterodimeric FACT complex (Spt16p-Pob3p), which associates with chromatin via interaction with Nhp6Ap and Nhp6Bp, and reorganizes nucleosomes to facilitate access to DNA by RNA and DNA polymerases
WormBase WBGene00001974 3.998479955315009e-46
Description: locus:hmg-4 FACT complex subunit SSRP1-A status:Confirmed UniProt:P41848 protein_id:CCD69078.1
DictyBase DDB_G0290331 3.998777993244535e-41
Description: ssrp1 on chromosome: 5 position 3969916 to 3971699
Stentor Coeruleus SteCoe_35967 1.462486227251231e-31
Description: None

General Information

No. Gene Name(s) Paragraph Text
129 POB3 The FACT-complex is a critical transcription regulator and an H2A/H2B chaperone. Affinity purification followed by mass spectrometry (AP-MS) of endogenously tagged T. thermophila histones H2A/H2B identified Cet1 and Pob3 subunits of the FACT complex as high-confidence interacting partners. Indirect immunofluorescence studies using endogenously tagged Cet1 indicated that it localized to both the MAC and MIC during vegetative growth. AP-MS analysis using Tetrahymena Cet1 as a bait protein revealed Pob3 and subunits of RNA polymerase as high-confidence co-purifying proteins (Ashraf et al. 2019).

Associated Literature

  1. Ref:30796450: Ashraf K, Nabeel-Shah S, Garg J, Saettone A, Derynck J, Gingras AC, Lambert JP, Pearlman RE, Fillingham J (2019) Proteomic analysis of histones H2A/H2B and variant Hv1 in Tetrahymena thermophila reveals an ancient network of chaperones. Molecular biology and evolution ( ):

Sequences 

>TTHERM_00049080(coding)
ATGAGTGATAGGTGTTATAAAGGTGTCAACTGGGGTAATGTTACCTTTGAAGATGACTAA
TTAGTCCTTAGTCATAACAAGAGAAGATTGTGCAAACTTTCTTTAAAGAAATTCACAAAC
TCTACTGTTAACAAAACTGATATTGTAATTGATTTGAACACAGTTGATCTTCAAGATGAT
GAAGACTAACTCTGTGAAATGAGATTATTTATTCCTCAATAATAAGACGCTTAAATGAAA
CCTGAAGATGATGGAGAAGGTGAAGAAAAAGAAGGTGAAGATAGCGTAGGTGGATATGCA
GATTAACTTAACTCTGAAATTATTACTAAAGCTAAAATTGGCTAATACTCTGGTTAATCA
ATAGTTAAATTCGAAGACATATCACTTCTTGTTCCTAGAGGTAAATACCAACTTGATATG
TACAAAAAAACAGTAAGATTCCACGGCTCTTCTTTTAACTATATAGTAGAATATAGTAAT
GTTATTAAGGGCTTCCTCCTTCCTCAACCTGACGAAGTACATGTTGCTTTCGTTCTTGGT
TTAGATTAACCCTTAAAAATTGGTAATACTGTTCACTCTTACATAGTTATGCAATTCAAA
AAAGAACAAAAGGCTAACATCAAAGTAAACATTGATCCTGAAGAAAAGAAGGAAGATAAA
TTAAAGGATCTCGATGAAGAATATGATGGATTCTTATATGAAATTGCTGGTTAATTGTTT
AAAACTTTATGTAATAATGTATAAATCATCATGCCTGCTGGTTTCTAAAGCTCTGATAAG
TAAAATTGCTTGAAATGTACCCTTAAAACTCACCAAGGTCTTTTATATCCTATGAGAAAG
AGTTTGATCTTTATATATAAACCTGTAATTCACATATAAATCAGTGATATTTAAAAGGTC
GAATTTAATAGAGTTGGTAATGCTACTCTGAATAAATTGTTTGATGTCAAAGTATTTACT
AAGACCACAACCCCTCAATTCTTTGGTTTTGAAAGAAAGGAATTAGATGTCCTCTTAGAA
TACTTTAAATCTAAAAATATTAAGATTACATATGATGATACTAATTAAGGAGCCACTTTT
GATGATGAGGATGAGGAATTCACAGACTCTATTTCTGAAGATGAGGAAGAAGGTAAGAGA
GCTCAAAGAAAGAGTGCTAAGAGAGCTAATAAAGCAGTAAAAGGATAATCAGGATTACCC
CTTGACGATGATGACGAAGAAGAAGACGAAGACTTTGATGCTGATGAATTTGAAAGTGAA
GATGATGAGGAAGAGGAAGAAGACGAAGATGAAGATGAAGATTTTGAATAATGA


>TTHERM_00049080(gene)
ATGAGTGATAGGTGTTATAAAGGTGTCAACTGGGGTAATGTTACCTTTGAAGATGACTAA
TTAGTCCTTAGTCATAACAAGAGAAGATTGTGCAAACTTTCTTTAAAGAAATTCACAAAC
TCTACTGTTAACAAAACTGATATTGTAATTGATTTGAACACAGTTGATCTTCAAGATGAG
TATCTTCATTTTTTTATTTTCCTCTAGATAGAAAAAAGATTCAAAAAATGTTTATTTTTA
CTTCAATTTTTGAAAAATAATGAATAAATTTACACAGAAAAGCCATTTTAGCTAATATTT
TATGCATATAATTATAGTGAAGACTAACTCTGTGAAATGAGATTATTTATTCCTCAATAA
TAAGACGCTGTAAAAATTAGTATTTAGTTAATGTAATTTTCTCAAAAACAATGTATTAAT
TCAAAAGTAAATGAAACCTGAAGATGATGGAGAAGGTGAAGAAAAAGAAGGTGAAGATAG
CGTAGGTGGATATGCAGATTAACTTAACTCTGAAATTATTACTAAAGCTAAAATTGGCTA
ATACTCTGGTTAATCAATAGTTAAATTCGAAGACATATCACTTCTTGTTCCTAGGTAAAT
TTTAAAACAAATTATCTAATTAGCTTCACATGCATTATGTTATACCACTTCCTAGGCCCA
ATAATATAAAATAATAAGTTTAAAAGTTTAAACATTATGAAAAATTGGCGAATAGTTTGC
TTAGAAGTTATAGCATTTTTTATATATATAAAATAATATGTCTATATTAAGAAATAGGAT
TATGACAAAATTTTAGCAGACTTTATTTAAATCTTAAAATTTTATAAATATAATAGAGGT
AAATACCAACTTGATATGTACAAAAAAACAGTAAGATTCCACGGCTCTTCTTTTAACTAT
ATAGTAGAATATAGTAATGTTATTAAGGGCTTCCTCCTTCCTCAACCTGACGAAGTAAGA
AGATTAAAAAGAGTTATAAGACAATTATAAATCATATTTTAAAAAAGGTACATGTTGCTT
TCGTTCTTGGTTTAGATTAACCCTTAAAAATTGGTAATACTGTTCACTCTTACATAGTTA
TGCAATTCAAAAAAGAACAAAAGGCTAACATCAAAGTAAACATTGATCCTGAAGAAAAGA
AGGAAGATAAATTAAAGGATCTCGATGAAGAATATGATGGATTCTTATATGAAATTGCTG
GTTAATTGTTTAAAACTTTATGTAATAATGTATAAATCATCATGCCTGCTGGTTTCTAAA
GGTAAACAATTAATTTTTTATTTTAGTTGAGTTAAATATAATTTAATAAATAACTAGCTC
TGATAAGTAAAATTGCTTGAAATGTACCCTTAAAACTCACCAAGGTCTTTTATATCCTAT
GAGAAAGAGTTTGATCTTTATATATAAACCTGTAATTCACATATAAATCAGTGATATTTA
AAAGGTCGAATTTAATAGAGTAAAATGAAATTTTTTGTCTAGAAATATTTTATTGAACAA
TTTGCCAAATAATAAAAAAAAATAGGTTGGTAATGCTACTCTGAATAAATTGTTTGATGT
CAAAGTATTTACTAAGACCACAACCCCTCAATTCTTTGGTTTTGAAAGAAAGGAATTAGA
TGTCCTCTTAGAATACTTTAAATCTAAAAATATTAAGATTACATATGATGATACTAATTA
AGGAGCCACTTTTGATGATGAGGATGAGGAATTCACAGACTCTATTTCTGAAGATGAGGA
AGAAGTATAATTTATCTTTTTATTTATTTCTGATAAAAATTTATATTGTAATTAAAGGGT
AAGAGAGCTCAAAGAAAGAGTGCTAAGAGAGCTAATAAAGCAGTAAAAGGATAATCAGGA
TTACCCCTTGACGATGATGACGAAGAAGAAGACGAAGACTTTGATGCTGATGAATTTGAA
AGTGAAGATGATGAGGAAGAGGAAGAAGACGAAGATGAAGATGAAGATTTTGAATAATGA


>TTHERM_00049080(protein)
MSDRCYKGVNWGNVTFEDDQLVLSHNKRRLCKLSLKKFTNSTVNKTDIVIDLNTVDLQDD
EDQLCEMRLFIPQQQDAQMKPEDDGEGEEKEGEDSVGGYADQLNSEIITKAKIGQYSGQS
IVKFEDISLLVPRGKYQLDMYKKTVRFHGSSFNYIVEYSNVIKGFLLPQPDEVHVAFVLG
LDQPLKIGNTVHSYIVMQFKKEQKANIKVNIDPEEKKEDKLKDLDEEYDGFLYEIAGQLF
KTLCNNVQIIMPAGFQSSDKQNCLKCTLKTHQGLLYPMRKSLIFIYKPVIHIQISDIQKV
EFNRVGNATLNKLFDVKVFTKTTTPQFFGFERKELDVLLEYFKSKNIKITYDDTNQGATF
DDEDEEFTDSISEDEEEGKRAQRKSAKRANKAVKGQSGLPLDDDDEEEDEDFDADEFESE
DDEEEEEDEDEDEDFEQ