Identifiers and Description

Gene Model Identifier

TTHERM_00079520

Standard Name

GTU1 (Gamma-TUbulin 1)

Aliases

PreTt10197 | 5.m00548 | 3828.m01461

Description

GTU1 tubulin gamma chain; Gamma-tubulin; GTU1p is essential; localizes to microtubule-organizing centers in vegetative cells: basal bodies, macronuclear envelopes, micronuclear envelopes, and contractile vacuole; Tubulin

Genome Browser (Macronucleus)

Full Screen View

No Genome Browser Data Present

Genome Browser (Micronucleus)

Full Screen View

No Genome Browser Data Present

External Links

• Uniprot
• Alphafold

Gene Ontology Annotations

Cellular Component

• contractile vacuole pore (IDA) | GO:0031913
• gamma-tubulin complex (ISS) | GO:0000930
• macronucleus (IDA) | GO:0031039
• micronucleus (IDA) | GO:0031040
• microtubule basal body (IDA) | GO:0005932
• nuclear envelope (IDA) | GO:0005635
• oral apparatus (IDA) | GO:0031912

Molecular Function

• structural constituent of cytoskeleton (ISS) | GO:0005200

Biological Process

• ciliary basal body duplication (IMP) | GO:0032054
• microtubule nucleation (ISS) | GO:0007020

Domains

• ( PF00091 ) Tubulin/FtsZ family, GTPase domain
• ( PF03953 ) Tubulin C-terminal domain

Gene Expression Profile

Vegetative Cell Cycle (Zhang et al., 2023)

GeneMania

No results were calculated for this gene in GeneMania.

Tetrahymena Stock Center

• ( SD01215 ) Macronucleus: GTU1 gene completely replaced by GTU1 gene with HA tag at the C terminus. Also has neo2 cassette in the 3' flanking region
• ( SD01340 ) Micronucleus: GTU1 replaced by neo2 Macronucleus: GTU1with c-terminal HA tag into MTT1 locus, partial replacement
• ( SD01341 ) Micronucleus: GTU1 replaced by neo2 Macronucleus: GTU1with c-terminal HA tag into MTT1 locus, partial replacement
• ( SD01342 ) Micronucleus: GTU1 replaced by neo2 Macronucleus: GTU1with c-terminal HA tag into MTT1 locus, partial replacement
• ( SD01363 ) Micronucleus: Neo2 into GTU1 locus Macronucleus: GTU1 with mutation T146V and c-terminus HA tag into the MTT1 locus, partial replacement.
• ( SD01364 ) Micronucleus: Neo2 into GTU1 locus Macronucleus: GTU1 with mutation T146V and c-terminus HA tag into the MTT1 locus, partial replacement.
• ( SD01365 ) Macronucleus: GTU1 with mutation T146G and c-terminal HA tag into the BTU1 locus under control of MTT1 promoter. Not sure if complete replacement.
• ( SD01366 ) Macronucleus: GTU1 with mutation T146G and c-terminal HA tag into the BTU1 locus under control of MTT1 promoter. Not sure if complete replacement.
• ( SD01367 ) Micronucleus: Neo2 replaces coding of GTU1
• ( SD01368 ) Micronucleus: Neo2 replaces coding of GTU1
• ( SD01369 ) Micronucleus: Neo2 replaces coding of GTU1
• ( SD01412 ) Macronucleus: HA tag at c-terminus of GTU1 with neo2 in 3' flanking region
• ( SD01413 ) Macronucleus: HA tag at c-terminus of GTU1 with neo2 in 3' flanking region
• ( SD01414 ) Macronucleus: HA tag at c-terminus of GTU1 with neo2 in 3' flanking region
• ( SD01415 ) Micronucleus: GTU1 replaced by neo2 Macronucleus: GTU1 into MTT1 locus, partial replacement
• ( SD01416 ) Micronucleus: GTU1 replaced by neo2 Macronucleus: GTU1 into MTT1 locus, partial replacement
• ( SD01417 ) Micronucleus: GTU1 replaced by neo2 Macronucleus: GTU1with c-terminal GFP tag into MTT1 locus, partial replacement
• ( SD02043 ) Micronucleus: Neo2 into GTU1 locus Macronucleus: GTU1 with mutations A101G and c-terminus HA tag into the MTT1 locus, partial replacement.
• ( SD02044 ) Micronucleus: Neo2 into GTU1 locus Macronucleus: GTU1 with mutations D124-A and E126-A and HA tag at c-teminus into the MTT1 locus, partial replacement.
• ( SD02045 ) Micronucleus: Neo2 into GTU1 locus Macronucleus: GTU1 with mutations D124-A and E126-A and HA tag at c-teminus into the MTT1 locus, partial replacement.
• ( SD02047 ) Micronucleus: Neo2 into GTU1 locus Macronucleus: GTU1 with 3HA tags at c-teminus into the MTT1 locus, partial replacement.
• ( SD02048 ) Micronucleus: Neo2 into GTU1 locus Macronucleus: GTU1 with 3HA tags at c-teminus into the MTT1 locus, partial replacement.
• ( SD02051 ) Micronucleus: Neo2 into GTU1 locus Macronucleus: GTU1 with mutations D124K and HA tag at c-teminus into the MTT1 locus, partial replacement.
• ( SD02052 ) Micronucleus: Neo2 into GTU1 locus Macronucleus: GTU1 with mutations D124K into the MTT1 locus, partial replacement.
• ( SD02057 ) Micronucleus: Neo2 into GTU1 locus Macronucleus: GTU1 with mutation K192A R193A into the MTT1 locus, partial replacement.
• ( SD02058 ) Micronucleus: Neo2 into GTU1 locus Macronucleus: GTU1 with mutation K192A R193A and C-terminal HA tag into the MTT1 locus, partial replacement.
• ( SD02143 ) Micronucleus: neo2 Macronucleus: Into MTT1 locus, GTU1 with HA and TAP tag
• ( SD02144 ) Micronucleus: neo2 Macronucleus: Into MTT1 locus, GTU1 with HA and TAP tag
• ( SD02145 ) Micronucleus: neo2 Macronucleus: Into MTT1 locus, GTU1 with HA and TAP tag
• ( SD02146 ) Micronucleus: neo2 Macronucleus: Into MTT1 locus, GTU1 with HA and TAP tag
• ( SD02147 ) Micronucleus: neo2 Macronucleus: Into MTT1 locus, GTU1 with HA and TAP tag
• ( SD02192 ) Micronucleus: Neo2 into GTU1 locus Macronucleus: GTU1 with mutation T270A,Y272A. Not sure if complete replacement.
• ( SD02193 ) Micronucleus: Neo2 into GTU1 locus Macronucleus: GTU1 with mutation T270A,Y272A. Not sure if complete replacement.
• ( SD02194 ) Micronucleus: Neo2 into GTU1 locus Macronucleus: GTU1 with mutations T270A, Y272A anc c-terminus HA tag into the MTT1 locus, partial replacement.
• ( SD02195 ) Micronucleus: Neo2 into GTU1 locus Macronucleus: GTU1 with mutations T270A, Y272A anc c-terminus HA tag into the MTT1 locus, partial replacement.
• ( SD02210 ) Micronucleus: Neo2 into GTU1 locus Macronucleus: GTU1 with mutation D116A,D117A and c-terminus HA tag into the MTT1 locus, partial replacement.
• ( SD02211 ) Micronucleus: Neo2 into GTU1 locus Macronucleus: GTU1 with mutation D116A,D117A and c-terminus HA tag into the MTT1 locus, partial replacement.
• ( SD02292 ) Micronucleus: Neo2 into GTU1 locus Macronucleus: GTU1 with mutations D124-A and E126-A and HA tag at c-teminus into the MTT1 locus, partial replacement.
• ( SD02293 ) Micronucleus: Neo2 into GTU1 locus Macronucleus: GTU1 with mutations D124-A and E126-A and HA tag at c-teminus into the MTT1 locus, partial replacement.
• ( SD02306 ) Macronucleus: GTU1 with c-terminal HA tag into the BTU1 locus under control of MTT1 promoter. Not sure if complete replacement.
• ( SD02307 ) Macronucleus: GTU1 with mutation T146G and c-terminal HA tag into the BTU1 locus under control of MTT1 promoter. Not sure if complete replacement.
• ( SD02308 ) Micronucleus: Neo2 into GTU1 locus Macronucleus: GTU1 with mutations K358A,K359A anc c-terminus HA tag into the MTT1 locus, partial replacement.
• ( SD02473 ) Micronucleus: Neo2 into GTU1 locus Macronucleus: GTU1 with mutation K286E and HA tag at c-teminus into the MTT1 locus, partial replacement.
• ( SD02474 ) Micronucleus: Neo2 into GTU1 locus Macronucleus: GTU1 with mutation K286E and HA tag at c-teminus into the MTT1 locus, partial replacement.
• ( SD02516 ) Micronucleus: Neo2 into GTU1 locus Macronucleus: GTU1 with mutation R285D,K286D and HA tag at c-teminus into the MTT1 locus, partial replacement.
• ( SD02531 ) Micronucleus: Neo2 into GTU1 locus Macronucleus: GTU1 with mutation T146V and c-terminus HA tag into the MTT1 locus, partial replacement.

Homologs

No Data fetched for Homologs

General Information

No Data fetched for General Information

Associated Literature

1. Ref:25660693: Kushida Y, Takaine M, Nakano K, Sugai T, Numata O (2015) Dynamic Change of Cellular Localization of Microtubule-Organizing Center During Conjugation of Ciliate Tetrahymena thermophila. Zoological science 32(1):25-32
2. Ref:21246753: Kushida Y, Nakano K, Numata O (2011) Amitosis requires ?-tubulin-mediated microtubule assembly in Tetrahymena thermophila. Cytoskeleton (Hoboken, N.J.) 68(2):89-96
3. Ref:16344310: Shang Y, Tsao CC, Gorovsky MA (2005) Mutational analyses reveal a novel function of the nucleotide-binding domain of gamma-tubulin in the regulation of basal body biogenesis. The Journal of cell biology 171(6):1035-44
4. Ref:12356864: Shang Y, Li B, Gorovsky MA (2002) Tetrahymena thermophila contains a conventional gamma-tubulin that is differentially required for the maintenance of different microtubule-organizing centers. The Journal of cell biology 158(7):1195-206
5. Ref:11891286: Shang Y, Song X, Bowen J, Corstanje R, Gao Y, Gaertig J, Gorovsky MA (2002) A robust inducible-repressible promoter greatly facilitates gene knockouts, conditional expression, and overexpression of homologous and heterologous genes in Tetrahymena thermophila. Proceedings of the National Academy of Sciences of the United States of America 99(6):3734-9
6. Ref:8864661: Liang A, Ruiz F, Heckmann K, Klotz C, Tollon Y, Beisson J, Wright M (1996) Gamma-tubulin is permanently associated with basal bodies in ciliates. European journal of cell biology 70(4):331-8
7. Ref:8124706: Stearns T, Kirschner M (1994) In vitro reconstitution of centrosome assembly and function: the central role of gamma-tubulin. Cell 76(4):623-37

Sequences

>TTHERM_00079520(coding)
ATGCCTAGAGATATAATTACACTTTAAGTCGGCCAATGCGGAAACTAAATTGGTATGGAA
TTTTGGAAATAGCTTTGTTTAGAGCATGGTATTAACCAAGAAGGTATTTTACAAGAATAT
GCAACAAACACTGATGACAGAAAAGAAGTTTTCTTTTATTAAGCAGATGATTAGCATTAT
ATTCCAAGGGCCTGTCTTATTGATTTAGAAGACAGAGTTCTAAAGAGAATAGTTAAATAA
TCTGATTATAGTTCACTATATAACCCAGAAAATATTTTTTATGGAACTGATGGATCAGGT
GCTGGTAATGTGTGGGCATACGGTTATTAAGAAGCTAATAAAATCCAAGATGATCTTCTT
GATATGATTGACAGAGAAGCTGATACATCTGATAGTTTTGAAGCTTTTCTTTTGATTCAT
AGTATTGCAGGTGGTACTGGTTCAGGTGTTGGCTCTTATTTATTGGAGAAATTAAATGAT
AGATTCCCTAAAAAAATTATTAAGACTTACTCTGTTTTTCCTAGTGGACACACAGATGGT
GTCGTACATCCTTATAACTCTCTTTTAACTTTAAAGAGACTTGCTTTGAATGCAGATGCA
ACCACTGTTTTAGATAATAACGCTTTAGAAAAAATAGCACAAGACAGATTAAAATTGGAA
AATCCTACAATTGAAACCACAAACTCACTTGTCTCAACAGTTATGGCAGCATGTACTACC
ACACTCAGATACCCTGGTTATATGAATAACGATTTAGTTGGTTTACTTGCATCTCTTGTT
CCTACACCTAAATGTCATTTCTTAATGACTGGATATACTCCTATCTCTATTGATAGACAT
ATAAAAACTATTAGAAAGACTACTGTACTTGATGTTATGAGAAGATTACTTTAAACAAAG
AATATCATGGTTAGTGCTTCAACTAAAAAGGGACAATATATTTCAATTATGAATGTAATT
CAAGGAGAAGTTGATGCATCTCAAATTCATAAATCACTTTAGCGTATTAGAGAAAGAAAA
TTAGCTAACTTTATTAACTGGGGACCTGCCTCCATACAAGTTGCATTATCTAAAAAATCA
CCATACATAGAAACATCTCATAAAGTAAGTGGTCTTATGCTCGCCAACCACACAAGCATC
CATACACTTTTTGAGAAGATTATCTCTAACTTTGACATAATGAGAAAAAAGAATGCTTAT
ATAGAATAGTTTAAAAAGGTTGATTCTTTTAACTTAGAGGAGTTCGATGATTCCAAGGAA
GTAGTACAATAATTAATAGAAGAATATAAAGCTGCTGAAGAACCTAACTATTTAGATTAC
TTCGATGATGGCTTGGAAGACGAAAACTGA

>TTHERM_00079520(gene)
GAGTGAGTGAGTGAATTGATATAAATAATATAATAGTGAGAGTTTTGCGCAAATTTTAAT
TTAAAAAGAAAAAATTTCAAATTTTATTGACATCAAAAGTAGAAAGAAGATCTTATTTAT
TGTTTGTGATTTATTAATTTGATAAAAATTCAAATAAATACTAAGAAACTTATAAAAAGG
AAAGCAAGTCAATAATTACAAGAGAACCGTTTTTATTAGCAAATTTATAAATAAAATGCC
TAGAGATATAATTACACTTTAAGTCGGCCAATGCGGAAACTAAATTGGTATGGAATTTTG
GAAATAGCTTTGTTTAGAGCATGGTATTAACCAAGAAGGTATTTTACAAGAATATGCAAC
AAACACTGATGACAGAAAAGAAGTTTTCTTTTATTAAGCAGATGATTAGCATTATATTCC
AAGGGCCTGTCTTATTGATTTAGAAGACAGAGTTCTAAAGAGAATAGTTAAATAATCTGA
TTATAGTTCACTATATAACCCAGAAAATATTTTTTATGGAACTGATGGATCAGGTGCTGG
TAATGTGTGGGCATACGGTTATTAAGAAGCTAATAAAATCCAAGATGATCTTCTTGATAT
GATTGACAGAGAAGCTGATACATCTGATAGTTTTGAAGCTTTTCTTTTGATTCATAGTAT
TGCAGGTGGTACTGGTTCAGGTGTTGGCTCTTATTTATTGGAGAAATTAAATGATAGATT
CCCTAAAAAAATTATTAAGACTTACTCTGTTTTTCCTAGTGGACACACAGATGGTGTCGT
ACATCCTTATAACTCTCTTTTAACTTTAAAGAGACTTGCTTTGAATGCAGATGCAACCAC
TGTTTTAGATAATAACGCTTTAGAAAAAATAGCACAAGACAGATTAAAATTGGAAAATCC
TACAATTGAAACCACAAACTCACTTGTCTCAACAGTTATGGCAGCATGTACTACCACACT
CAGATACCCTGGTTATATGAATAACGATTTAGTTGGTTTACTTGCATCTCTTGTTCCTAC
ACCTAAATGTCATTTCTTAATGACTGGATATACTCCTATCTCTATTGATAGACATATAAA
AACTATTAGAAAGACTACTGTACTTGATGTTATGAGAAGATTACTTTAAACAAAGAATAT
CATGGTTAGTGCTTCAACTAAAAAGGGACAATATATTTCAATTATGAATGTAATTCAAGG
AGAAGTTGATGCATCTCAAATTCATAAATCACTTTAGCGTATTAGAGAAAGAAAATTAGC
TAACTTTATTAACTGGGGACCTGCCTCCATACAAGTTGCATTATCTAAAAAATCACCATA
CATAGAAACATCTCATAAAGTAAGTGGTCTTATGCTCGCCAACCACACAAGCATCCATAC
ACTTTTTGAGAAGATTATCTCTAACTTTGACATAATGAGAAAAAAGAATGCTTATATAGA
ATAGTTTAAAAAGGTTGATTCTTTTAACTTAGAGGAGTTCGATGATTCCAAGGAAGTAGT
ACAATAATTAATAGAAGAATATAAAGCTGCTGAAGAACCTAACTATTTAGATTACTTCGA
TGATGGCTTGGAAGACGAAAACTGATTAAAAATAAAAAAACAATTGTAAAACAAACATTA
GCTCTGCTTGCTTAACCAAAACCTATATAGAATAATTTATGTTAACAATATCTATTAAAT
TTAATATAAATAAATAAACACTTAAATTAATTTTGCGTAATTTTATAATAATGTCTTATT
ATTTATTATATATATTTGTATACTATTAAAAACTATTCATAAAAATATTGCTACTCAAAT
TATATTTTCAAATTAAATTAATTCAAAAAGTTAACCAAGTCAT

>TTHERM_00079520(protein)
MPRDIITLQVGQCGNQIGMEFWKQLCLEHGINQEGILQEYATNTDDRKEVFFYQADDQHY
IPRACLIDLEDRVLKRIVKQSDYSSLYNPENIFYGTDGSGAGNVWAYGYQEANKIQDDLL
DMIDREADTSDSFEAFLLIHSIAGGTGSGVGSYLLEKLNDRFPKKIIKTYSVFPSGHTDG
VVHPYNSLLTLKRLALNADATTVLDNNALEKIAQDRLKLENPTIETTNSLVSTVMAACTT
TLRYPGYMNNDLVGLLASLVPTPKCHFLMTGYTPISIDRHIKTIRKTTVLDVMRRLLQTK
NIMVSASTKKGQYISIMNVIQGEVDASQIHKSLQRIRERKLANFINWGPASIQVALSKKS
PYIETSHKVSGLMLANHTSIHTLFEKIISNFDIMRKKNAYIEQFKKVDSFNLEEFDDSKE
VVQQLIEEYKAAEEPNYLDYFDDGLEDEN