Identifiers and Description

Gene Model Identifier

TTHERM_00144830

Standard Name

TWI11 (Tetrahymena piWI 11)

Aliases

PreTt26691 | 11.m00388 | 3702.m00110

Description

TWI11 piwi domain protein; Argonaute/Piwi family protein; Piwi domain

Genome Browser (Macronucleus)

Full Screen View


Genome Browser (Micronucleus)

Full Screen View

External Links

Gene Ontology Annotations

Cellular Component

Molecular Function

Biological Process

Domains

Gene Expression Profile

Vegetative Cell Cycle (Zhang et al., 2023)

GeneMania

Tetrahymena Stock Center

  • ( SD02064 )
  • ( SD02065 )
  • ( SD02066 ) Macronucleus: N-terminal HA tag on Twi 11
  • ( SD02067 ) Macronucleus: N-terminal HA tag on Twi 11
  • ( SD02114 )
  • ( SD02797 ) Micronucleus: HA tagged TWI11
  • ( SD02798 ) Micronucleus: HA tagged TWI11
  • ( SD02799 ) Micronucleus: Neo3 upstream of HA tagged TWI11
  • ( SD02800 ) Micronucleus: Neo3 upstream of HA tagged TWI11
  • ( SD02801 ) Micronucleus: TWI11-HA with neo3 in 5’ region Macronucleus: TWI11-HA with neo3 in 5’ region
  • ( SD02802 ) Micronucleus: TWI11-HA with neo3 in 5’ region Macronucleus: TWI11-HA with neo3 in 5’ region
  • ( SD02803 ) Micronucleus: TWI11-HA with neo3 in 5’ region Macronucleus: TWI11-HA with neo3 in 5’ region
  • ( SD02804 ) Micronucleus: TWI11-HA with neo3 in 5’ region Macronucleus: TWI11-HA with neo3 in 5’ region
  • ( SD02805 ) Micronucleus: TWI11-HA with neo3 in 5’ region Macronucleus: TWI11-HA with neo3 in 5’ region
  • ( SD02853 ) Macronucleus: TWI11 with neo3
  • ( SD02854 ) Micronucleus: Part of TWI11 coding replaced with neo3 cassette.
  • ( SD02855 ) Micronucleus: Part of TWI11 coding replaced with neo3 cassette, homozygous
  • ( SD02856 ) Micronucleus: Part of TWI11 coding replaced with neo3 cassette, heterozygous
  • ( SD02857 ) Micronucleus: Part of TWI11 coding replaced with neo3 cassette, homozygous
  • ( SD02858 ) Micronucleus: Neo 3 cassette replaces part of TWI11 coding region Macronucleus: Neo 3 cassette replaces part of TWI11 coding region
  • ( SD02860 ) Micronucleus: Neo3 replaces part of TWI11 Macronucleus: Neo3 replaces part of TWI11
  • ( SD02861 ) Micronucleus: Original ΔTWI11 mic replaced by the WT mic of a CU427 cell Macronucleus: Still has ΔTWI11 with neo3 replacing part of coding
  • ( SD02862 ) Micronucleus: neo3 in coding Macronucleus: neo3 in coding
  • ( SD02863 ) Macronucleus: TWI11 with neo3
  • ( SD02864 ) Macronucleus: TWI11 with neo3
  • ( SD02865 ) Macronucleus: TWI11 with neo3
  • ( SD02866 ) Macronucleus: neo3 replaces part of coding region

Homologs

No Data fetched for Homologs

General Information

No. Gene Name(s) Paragraph Text
2314 TWI11, TWI1 Maternally-expressed Twi1p is loaded with Early-scnRNAs expressed from the MIC at early conjugation stages while zygotically-expressed Twi1p and Twi11p are loaded with Late-scnRNAs expressed from the new MAC at late conjugation stages (Noto et al. 2015).

Associated Literature

  1. Ref:29887308: Noto T, Mochizuki K (2018) Small RNA-Mediated trans-Nuclear and trans-Element Communications in Tetrahymena DNA Elimination. Current biology : CB 28(12):1938-1949.e5
  2. Ref:29920263: Chalker DL (2018) Transgenerational Inheritance: Parental Guidance Suggested. Current biology : CB 28(12):R702-R704
  3. Ref:29021213: Noto T, Mochizuki K (2017) Whats, hows and whys of programmed DNA elimination in Tetrahymena. Open biology 7(10):
  4. Ref:27892853: Hamilton EP, Kapusta A, Huvos PE, Bidwell SL, Zafar N, Tang H, Hadjithomas M, Krishnakumar V, Badger JH, Caler EV, Russ C, Zeng Q, Fan L, Levin JZ, Shea T, Young SK, Hegarty R, Daza R, Gujja S, Wortman JR, Birren BW, Nusbaum C, Thomas J, Carey CM, Pritham EJ, Feschotte C, Noto T, Mochizuki K, Papazyan R, Taverna SD, Dear PH, Cassidy-Hanley DM, Xiong J, Miao W, Orias E, Coyne RS (2016) Structure of the germline genome of Tetrahymena thermophila and relationship to the massivel
  5. Ref:26095658: Noto T, Kataoka K, Suhren JH, Hayashi A, Woolcock KJ, Gorovsky MA, Mochizuki K (2015) Small-RNA-Mediated Genome-wide trans-Recognition Network in Tetrahymena DNA Elimination. Molecular cell 59(2):229-42
  6. Ref:19656801: Couvillion MT, Lee SR, Hogstad B, Malone CD, Tonkin LA, Sachidanandam R, Hannon GJ, Collins K (2009) Sequence, biogenesis, and function of diverse small RNA classes bound to the Piwi family proteins of Tetrahymena thermophila. Genes & development 23(17):2016-32

Sequences 

>TTHERM_00144830(coding)
ATGAGTTTTAACGATAATAGTAATACTTTGGTTACTAATCATTACAAAATTAAAATTAAT
AATATAAAGATTAATTGCTATGAATTTTAGACTTAGCCTCCTATTGATCCTCTTTTGCTT
AACCGTAAGTCAAGATACATCTCAAGAGTTTTCAACAAAAAGATAAAAGAAATATTTAGT
ACCTTTATTATTGGGTCTTAACTTATTTACTCTCCTATTGATTTGCATAATAAAGTCAGT
CTTGGAACCTTTGATTTTGTATCCCCTAAGACTGATGGGTGTGAGTCCTTCACTTAAGAG
CTTGAAGTTTTCTTAGTAAAGAAAAAAGATATTGTTGGATAATAGCTTGAAGAGGAAAAT
AAAGAACTTCAAAATATATTGGGATAGCTAATGAGAAACTTCTAAAATAACACTAAAGGG
TTTTAATAGCTTGGTGCTACTAAGAGGTTTGCTATGAATGTTATAAAAAATATCTCTAAT
GGCCTTGAAGCAGTTCTCGGAGTTGAAACTTTCTTCAAATTTAGTCCTCAAACAGGATTT
TAACTTTCAGTAGATTACTGTATCAAAGTTATTAACAACACATCTGTTTATGATTTCTTA
AAAAAATCTATGTCTTAAGGAGCAGATAGTATCCAAGAAAAAATGTAAAACTGTGTAGTT
ATTAGTAATTATGGAGATAAAAAATTGTACAAGGTTAATGGAGTAGCGTTTGATTTAAAT
CCTAATTCTTTCTTTGAAATGAGGGATCCTAATGAATCTACTGGTAAAAAGAAAATATCA
TTCGTTTAATACTATGAGCTCGTCCATAACATCAGAATTAGAGATAATAGATAGCCTCTT
CTTATAGTTGAAAAGAAAAGAAGATAAAATAAAAGAGCTAATTAAGAAGGAAAACCATAA
TAAGAAACTCCTAAAATCTATTTAATACCAGAGTTATGCTAAATGACTGGCATTCCTGAT
GAACTTCAAAATGACTTTATGCTCAAGAAAGCTATAACACAAGTTTCCTAGCCCAAACCT
TAAGAGAGATCTTAGCACTGTGGAAATTATGTCTCAGCTCTAATACACTAAAACTAAAAG
GCTCTAAGTGAATGGGGCATTTAACTAAGCTAAAATGACCCTGTTAAAGCTCGTTATCAA
GTTATCCAACCAGGATGTATTCTTATGAATTAAAATAGAGAAATTAACATTTATGGAGAA
AAATAACTAGATAGAAAAATTTAAGATAGAATGTACCAGCCTTGCTACCTCAAGAAGTGG
TGTGCCATTTGTCACAAATCTGATCAATAATTGGCTGGCTAGTTCATGTCAACACTGAAG
AATAGAATGCAAGCTCATGGGATAGGATATAATGAACCTAATTATTTTACTGTTGAAGGT
TAATCACAAGATGAACTCAAAAGGCTTATCGATTCATTCCCACCTGATGTTGATTTTATT
GTATTTATATTGCAAGGAGCCAAAGGAAAAGGCAAAAATTATTAGTACCTAAAATCTTTT
TTGTTAAAAGAAAAACCTATTCCTAGCTAGATGATTCTTTAAGGTACTATCAAGAGCAGT
AAAGATGGTTGCTAAGTTATTTGTAACAAGATATGCAATCAAATATGTATTAAAGTTGGA
GGAATACCTTACATCATCAAAGATCTTCCATTTAGTAATCTTCCTACTATGCTTGTTGGT
ATAGATTACATCCGCAAAGAAAACTAAAAATCAGTTTATTCATTTGTTGCTTCAGTTGAC
AGTACATTCTGTAAATTTTTTTCAGGTGCTTAACTTTTAGACGCTTAAGACTAAAATAAC
AAATTTGTTGACAAGCTTTTGCAAGCAAGTCTTACTTAATTTAAATAAAAAAATGGAATA
CTGCCTTAACGTGTTATAATTTATAGATAAGCTGCTAACAATATATTGAACGAGCCTCTA
AAGGAAGAAATTGCAATCATGAAAAAATGCCTATCAGAAATGGAAATGTCTTAATGCAAG
ATCCACATAGTTGGTGTTAACAGTAGTGATAAGACTAAATTTTTCAATGAAGATTACTCT
AATCCAGCTTAAGGAACTGTCATTAGTTAAGATGTAGTATCATCGTAAGGAGATTTCTTC
TTGGTGTCTCAAAGATCTATGCAAGGAACTGCATAACCTATGATGTACAAAGAGATTTAC
AGTGATTATGAAGGTATGGAATTAGAATAAATAAGGACTTAACTTCATACCTTGACCTAT
AGGATGAGTTATCTTTTCTACAATTTCACTGGAGCAATCAAGATACCAAGCATCTTGAAG
TATGCTGAATAGTAAACTAAATTCATGAAAAACAACTTTGAGAACTTGAATAAGATGGAC
CCTCAAACACTCCTCAGCTAGAAGATGTGTGATAAGAATTCTACATTCTATATTTGA



>TTHERM_00144830(gene)
TCAGCACTTGAGTCAGATTTTTATAAATAATTAATTAATAAAAAACTATTTCATAAAAAT
TGTTTTAATCTTAAGTAAAAAATAATCTCTAAATTCGATATGAGTTTTAACGATAATAGT
AATACTTTGGTTACTAATCATTACAAAATTAAAATTAATAATATAAAGATTAATTGCTAT
GAATTTTAGACTTAGCCTCCTATTGATCCTCTTTTGCTTAACCGTAAGTCAAGATACATC
TCAAGAGTTTTCAACAAAAAGATAAAAGAAATATTTAGTACCTTTATTATTGGGTCTTAA
CTTATTTACTCTCCTATTGATTTGCATAATAAAGTCAGTCTTGGAACCTTTGATTTTGTA
TCCCCTAAGACTGATGGGTGTGAGTCCTTCACTTAAGAGCTTGAAGTTTTCTTAGTAAAG
AAAAAAGATATTGTTGGATAATAGCTTGAAGAGGAAAATAAAGAACTTCAAAATATATTG
GGATAGCTAATGAGAAACTTCTAAAATAACACTAAAGGGTTTTAATAGCTTGGTGCTACT
AAGAGGTTTGCTATGAATGTTATAAAAAATATCTCTAATGGCCTTGAAGCAGTTCTCGGA
GTTGAAACTTTCTTCAAATTTAGTCCTCAAACAGGATTTTAACTTTCAGTAGATTACTGT
ATCAAAGTTATTAACAACACATCTGTTTATGATTTCTTAAAAAAATCTATGTCTTAAGGA
GCAGATAGTATCCAAGAAAAAATGTAAAACTGTGTAGTTATTAGTAATTATGGAGATAAA
AAATTGTACAAGGTTAATGGAGTAGCGTTTGATTTAAATCCTAATTCTTTCTTTGAAATG
AGGGATCCTAATGAATCTACTGGTAAAAAGAAAATATCATTCGTTTAATACTATGAGCTC
GTCCATAACATCAGAATTAGAGATAATAGATAGCCTCTTCTTATAGTTGAAAAGAAAAGA
AGATAAAATAAAAGAGCTAATTAAGAAGGAAAACCATAATAAGAAACTCCTAAAATCTAT
TTAATACCAGAGTTATGCTAAATGACTGGCATTCCTGATGAACTTCAAAATGACTTTATG
CTCAAGAAAGCTATAACACAAGTTTCCTAGCCCAAACCTTAAGAGAGATCTTAGCACTGT
GGAAATTATGTCTCAGCTCTAATACACTAAAACTAAAAGGCTCTAAGTGAATGGGGCATT
TAACTAAGCTAAAATGACCCTGTTAAAGCTCGTTATCAAGTTATCCAACCAGGATGTATT
CTTATGAATTAAAATAGAGAAATTAACATTTATGGAGAAAAATAACTAGATAGAAAAATT
TAAGATAGAATGTACCAGCCTTGCTACCTCAAGAAGTGGTGTGCCATTTGTCACAAATCT
GATCAATAATTGGCTGGCTAGTTCATGTCAACACTGAAGAATAGAATGCAAGCTCATGGG
ATAGGATATAATGAACCTAATTATTTTACTGTTGAAGGTTAATCACAAGATGAACTCAAA
AGGCTTATCGATTCATTCCCACCTGATGTTGATTTTATTGTATTTATATTGCAAGGAGCC
AAAGGAAAAGGCAAAAATTATTAGTACCTAAAATCTTTTTTGTTAAAAGAAAAACCTATT
CCTAGCTAGATGATTCTTTAAGGTACTATCAAGAGCAGTAAAGATGGTTGCTAAGTTATT
TGTAACAAGATATGCAATCAAATATGTATTAAAGTTGGAGGAATACCTTACATCATCAAA
GATCTTCCATTTAGTAATCTTCCTACTATGCTTGTTGGTATAGATTACATCCGCAAAGAA
AACTAAAAATCAGTTTATTCATTTGTTGCTTCAGTTGACAGTACATTCTGTAAATTTTTT
TCAGGTGCTTAACTTTTAGACGCTTAAGACTAAAATAACAAATTTGTTGACAAGCTTTTG
CAAGCAAGTCTTACTTAATTTAAATAAAAAAATGGAATACTGCCTTAACGTGTTATAATT
TATAGATAAGCTGCTAACAATATATTGAACGAGCCTCTAAAGGAAGAAATTGCAATCATG
AAAAAATGCCTATCAGAAATGGAAATGTCTTAATGCAAGATCCACATAGTTGGTGTTAAC
AGTAGTGATAAGACTAAATTTTTCAATGAAGATTACTCTAATCCAGCTTAAGGAACTGTC
ATTAGTTAAGATGTAGTATCATCGTAAGGAGATTTCTTCTTGGTGTCTCAAAGATCTATG
CAAGGAACTGCATAACCTATGATGTACAAAGAGATTTACAGTGATTATGAAGGTATGGAA
TTAGAATAAATAAGGACTTAACTTCATACCTTGACCTATAGGATGAGTTATCTTTTCTAC
AATTTCACTGGAGCAATCAAGATACCAAGCATCTTGAAGTATGCTGAATAGTAAACTAAA
TTCATGAAAAACAACTTTGAGAACTTGAATAAGATGGACCCTCAAACACTCCTCAGCTAG
AAGATGTGTGATAAGAATTCTACATTCTATATTTGACTGCAATTATAAAATAAAATAATT
AAAAAGATGATATTTAATTACAATTAATAATTAATATTTTGTATTAATATTTATGTTTCT
ATGCACTTATGTAATTAATTTATCATTTTTTTAATTAAAATAATTCTCATTTAATATTTT
TAAAAATCAATAAAATATTTGTTTCTCCTTACTTAAGTTAGATAAAGAAAAATAATTAAT
TATTTTTAATTAATTATAATAAGAAAAACTAAAATCTCCATAAATTGCTAACACTATTTC
AGAAAAGTAATTCATTTCA



>TTHERM_00144830(protein)
MSFNDNSNTLVTNHYKIKINNIKINCYEFQTQPPIDPLLLNRKSRYISRVFNKKIKEIFS
TFIIGSQLIYSPIDLHNKVSLGTFDFVSPKTDGCESFTQELEVFLVKKKDIVGQQLEEEN
KELQNILGQLMRNFQNNTKGFQQLGATKRFAMNVIKNISNGLEAVLGVETFFKFSPQTGF
QLSVDYCIKVINNTSVYDFLKKSMSQGADSIQEKMQNCVVISNYGDKKLYKVNGVAFDLN
PNSFFEMRDPNESTGKKKISFVQYYELVHNIRIRDNRQPLLIVEKKRRQNKRANQEGKPQ
QETPKIYLIPELCQMTGIPDELQNDFMLKKAITQVSQPKPQERSQHCGNYVSALIHQNQK
ALSEWGIQLSQNDPVKARYQVIQPGCILMNQNREINIYGEKQLDRKIQDRMYQPCYLKKW
CAICHKSDQQLAGQFMSTLKNRMQAHGIGYNEPNYFTVEGQSQDELKRLIDSFPPDVDFI
VFILQGAKGKGKNYQYLKSFLLKEKPIPSQMILQGTIKSSKDGCQVICNKICNQICIKVG
GIPYIIKDLPFSNLPTMLVGIDYIRKENQKSVYSFVASVDSTFCKFFSGAQLLDAQDQNN
KFVDKLLQASLTQFKQKNGILPQRVIIYRQAANNILNEPLKEEIAIMKKCLSEMEMSQCK
IHIVGVNSSDKTKFFNEDYSNPAQGTVISQDVVSSQGDFFLVSQRSMQGTAQPMMYKEIY
SDYEGMELEQIRTQLHTLTYRMSYLFYNFTGAIKIPSILKYAEQQTKFMKNNFENLNKMD
PQTLLSQKMCDKNSTFYI