Identifiers and Description

Gene Model Identifier

TTHERM_00146340

Standard Name

CNA1 (CENP-A-like 1)

Aliases

PreTt26841 | 11.m00538 | 3702.m00260

Description

CNA1 histone H3-like centromeric protein A; Centromeric histone similar to CENP-A; localized to peripheral centromeres in micronucleus; knockouts show abnormal mitotic segregation of micronucleus- leading to amicronucleate progeny

Genome Browser (Macronucleus)

Full Screen View

No Genome Browser Data Present

Genome Browser (Micronucleus)

Full Screen View

No Genome Browser Data Present

External Links

• Uniprot
• Alphafold

Gene Ontology Annotations

Cellular Component

• chromosome, centromeric region (IDA) | GO:0000775
• micronucleus (IDA) | GO:0031040
• nuclear nucleosome (ISS) | GO:0000788

Molecular Function

• centromeric DNA binding (ISS) | GO:0019237

Biological Process

• meiotic prophase I (EXP) | GO:0007128
• mitotic sister chromatid segregation (IMP) | GO:0000070

Domains

• ( PF00125 ) Core histone H2A/H2B/H3/H4
• ( PF00808 ) Histone-like transcription factor (CBF/NF-Y) and

Gene Expression Profile

Vegetative Cell Cycle (Zhang et al., 2023)

GeneMania

No results were calculated for this gene in GeneMania.

Tetrahymena Stock Center

• ( SD01801 ) Micronucleus: DCL1 replaced by neo3 Macronucleus: CNA1 GFP tagged replaces MTT1 coding in MTT1 locus, bsr1 cassete in MTT1 5’
• ( SD01802 ) Micronucleus: DCL1 replaced by neo3 Macronucleus: CNA1 GFP tagged replaces MTT1 coding in MTT1 locus, bsr1 cassete in MTT1 5’
• ( SD01803 ) Micronucleus: DCL1 replaced by neo3 Macronucleus: CNA1 GFP tagged replaces MTT1 coding in MTT1 locus, bsr1 cassete in MTT1 5’
• ( SD01804 ) Micronucleus: DCL1 replaced by neo3 Macronucleus: CNA1 GFP tagged replaces MTT1 coding in MTT1 locus, bsr1 cassete in MTT1 5’
• ( SD01805 ) Micronucleus: DCL1 replaced by neo3 Macronucleus: CNA1 GFP tagged replaces MTT1 coding in MTT1 locus, bsr1 cassete in MTT1 5’
• ( SD01806 ) Micronucleus: DCL1 replaced by neo3 Macronucleus: CNA1 GFP tagged replaces MTT1 coding in MTT1 locus, bsr1 cassete in MTT1 5’
• ( SD01807 ) Micronucleus: DCL1 replaced by neo3 Macronucleus: CNA1 GFP tagged replaces MTT1 coding in MTT1 locus, bsr1 cassete in MTT1 5’
• ( SD01808 ) Micronucleus: TWI11replaced by neo3 Macronucleus: CNA1 GFP tagged replaces MTT1 coding in MTT1 locus, bsr1 cassete in MTT1 5’
• ( SD01809 ) Micronucleus: TWI11replaced by neo3 Macronucleus: CNA1 GFP tagged replaces MTT1 coding in MTT1 locus, bsr1 cassete in MTT1 5’
• ( SD01810 ) Micronucleus: TWI11 replaced by neo3 Macronucleus: CNA1 GFP tagged replaces MTT1 coding in MTT1 locus, bsr1 cassete in MTT1 5’
• ( SD01811 ) Micronucleus: TWI11 replaced by neo3 Macronucleus: CNA1 GFP tagged replaces MTT1 coding in MTT1 locus, bsr1 cassete in MTT1 5’
• ( SD01812 ) Macronucleus: GFP tagged CNA1 from the MTT1 locus
• ( SD01813 ) "Micronucleus: ∆hht1 ; ∆hht2 ∆hhf1; ∆hhf2 Macronucleus: hybrid H3 replaces major H3. In MTT1 locus,GFP tagged CNA1"
• ( SD01932 ) Micronucleus: Neo2 into CNA1
• ( SD01933 ) Micronucleus: Neo2 into CNA1
• ( SD01934 ) Micronucleus: Neo2 into CNA1
• ( SD02693 ) Macronucleus: neo2 into CNA1

Homologs

Source	Identifier	Score
Tetrahymena borealis	EI9_04648.1	3.999498296699862e-96
	Description: core histone H2A/H2B/H3/H4 family protein (187 aa)
DictyBase	DDB_G0267402	3.00033207980219e-32
	Description: H3a on chromosome: 1 position 1520754 to 1521173
Oxytricha	Contig9358.0.g54	1.000137892321693e-31
	Description: Core histone H2A/H2B/H3/H4
WormBase	WBGene00012276	4.9985000769285e-31
	Description: locus:his-74 Histone H3.3-like type 2 status:Confirmed UniProt:Q27532 protein_id:CAA98963.1
SGD	YNL031C	4.000352986427571e-28
	Description: HHT2 Histone H3, core histone protein required for chromatin assembly, part of heterochromatin-mediated telomeric and HM silencing; one of two identical histone H3 proteins (see HHT1); regulated by acetylation, methylation, and phosphorylation
Stentor Coeruleus	SteCoe_15287	0.000000000000012664165549094176
	Description: None

General Information

No Data fetched for General Information

Associated Literature

1. Ref:22976299: Loidl J, Lukaszewicz A, Howard-Till RA, Koestler T (2012) The Tetrahymena meiotic chromosome bouquet is organized by centromeres and promotes interhomolog recombination. Journal of cell science 125(Pt 23):5873-80
2. Ref:16251352: Cervantes MD, Xi X, Vermaak D, Yao MC, Malik HS (2006) The CNA1 histone of the ciliate Tetrahymena thermophila is essential for chromosome segregation in the germline micronucleus. Molecular biology of the cell 17(1):485-97
3. Ref:16738316: Cui B, Gorovsky MA (2006) Centromeric histone H3 is essential for vegetative cell division and for DNA elimination during conjugation in Tetrahymena thermophila. Molecular and cellular biology 26(12):4499-510
4. Ref:15701804: Liu Y, Song X, Gorovsky MA, Karrer KM (2005) Elimination of foreign DNA during somatic differentiation in Tetrahymena thermophila shows position effect and is dosage dependent. Eukaryotic cell 4(2):421-31
5. Ref:15598983: Mochizuki K, Gorovsky MA (2005) A Dicer-like protein in Tetrahymena has distinct functions in genome rearrangement, chromosome segregation, and meiotic prophase. Genes & development 19(1):77-89

Sequences

>TTHERM_00146340(coding)
ATGGCTAGGAAAGCTTATTAACCAAAAAGGAGGAGTAATTCAAATCAAAATTAATAGAGA
TCTGATAGCCTGAAAAAAAATAAATAGGATAATTTGAGATCTAAATCTGCTGGGAATTAA
TAAGGGAATGAAAAAAACAAAAAAGATATTCAAGACCAAAGAAATAAAGCAAGTACAAAA
AAGAAAAGGGAGTCATCAGGAGAAAAATATGAGTCTGCAAGAGATAAAGTTATTAGAAGA
TTTAGGCCAGGGGATAATGCCCTTAAGCAATTAAGATAATATAATTAGACTCCATCATTG
CTAATAAGAAAATTACCCTTTTAGAGGTTAATAAGAGAGATTTCTACAAGAATGACAGAA
GAAGATTCGCTGAGATGGACAAGTTTTGCTCTGGTTTTACTTTAAACAGTTGTAGAAGAT
TATATGGTTTCATTTTTTGAAGATGCGAATGCTTGTGCTCTCCATGCTAAAAGAGTTACT
CTCATGTCTAAAGATCTTGCTCTTGCGGCAAGAATTAGGGGATAAAAAAATGTTACAGGT
ATATTTATCCCTACTAAAAAATGA

>TTHERM_00146340(gene)
ATGGCTAGGAAAGCTTATTAACCAAAAAGGAGGAGTAATTCAAATCAAAATTAATAGAGA
TCTGATAGCCTGAAAAAAAATAAATAGGATAATTTGAGATCTAAATCTGCTGGGAATTAA
TAAGGGAATGAAAAAAACAAAAAAGATATTCAAGACCAAAGAAATAAAGCAAGTACAAAA
AAGAAAAGGGAGTCATCAGGAGAAAAATATGAGTCTGCAAGAGATAAAGTTATTAGAAGA
TTTAGGCCAGGGGATAATGCCCTTAAGCAATTAAGATAATATAATTAGACTCCATCATTG
CTAATAAGAAAATTACCCTTTTAGAGGTTAATAAGAGAGATTTCTACAAGAATGACAGAA
GAAGATTCGCTGAGATGGACAAGTTTTGCTCTGGTTTTACTTTAAACAGTTGTAGAAGAT
TATATGGTTTCATTTTTTGAAGATGCGAATGCTTGTGCTCTCCATGCTAAAAGAGTTACT
CTCATGTCTAAAGATCTTGCTCTTGCGGCAAGAATTAGGGGATAAAAAAATGTTACAGGT
ATATTTATCCCTACTAAAAAATGA

>TTHERM_00146340(protein)
MARKAYQPKRRSNSNQNQQRSDSLKKNKQDNLRSKSAGNQQGNEKNKKDIQDQRNKASTK
KKRESSGEKYESARDKVIRRFRPGDNALKQLRQYNQTPSLLIRKLPFQRLIREISTRMTE
EDSLRWTSFALVLLQTVVEDYMVSFFEDANACALHAKRVTLMSKDLALAARIRGQKNVTG
IFIPTKK