Identifiers and Description

Gene Model Identifier

TTHERM_00191480

Standard Name

ASI2 (Anlagen Stage Induced 2)

Aliases

PreTt08799 | 15.m00510 | 3697.m00221

Description

ASI2 anlagen stage induced 1 protein; Similar to anlagen stage induced protein 1 (ASI1); protein of unknown function; gene is located adjacent to the ASI1 gene

Genome Browser (Macronucleus)

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Genome Browser (Micronucleus)

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Gene Ontology Annotations

Cellular Component

Molecular Function

Biological Process

Domains

  • ( PF00672 ) HAMP domain
  • ( PF02326 ) YMF19 hypothetical plant mitochondrial protein
  • ( PF02743 ) Cache domain

Gene Expression Profile

Tetrahymena Stock Center

  • ( SD01594 ) Macronucleus: ASI2 replaced with neo2 cassette
  • ( SD01595 ) Macronucleus: ASI2 replaced with neo2 cassette
  • ( SD01596 )
  • ( SD01597 )
  • ( SD03069 ) Micronucleus: heterozygous for ASI2 knockout
  • ( SD03070 ) Micronucleus: ASI2
  • ( SD03071 ) Macronucleus: ASI2 somatic knockout
  • ( SD03072 ) Macronucleus: ASI2 knockout
  • ( SD03073 ) Macronucleus: ASI2 replaced with ASI2Y607F

Homologs

Source Identifier Score
Tetrahymena borealis EI9_06168.1 9.997093137033517e-262
Description: ASI2 protein (724 aa)

General Information

No. Gene Name(s) Paragraph Text
85 ASI2 Gene Information: ASI2 is a gene regulating endocycling in Tetrahymena thermophila. Though nonessential for vegative growth, it is upregulated after meiosis and is involved in the creation of a new MAC. Introduced via transduction with ASI2-GFP plasmid to establish parental cells that showed transcription of ASI2 occurs both in MAC and parental cells at conjugation.
86 ASI2 Nucleic Acid Interactions: ASI2p independent of ssRNA synthesis/accumulation
87 ASI2 Regulation & Expression: The Tetrahymena gene ASI1 (anlagen stage induced 1) was isolated from a cDNA library of genes that are up-regulated during development of the macronuclear anlagen. As its name implies, the abundance of ASI2 mRNA peaks at 9 h of mating, early in macronuclear anlagen development.

Associated Literature

  1. Ref:20656911: Yin L, Gater ST, Karrer KM (2010) A developmentally regulated gene, ASI2, is required for endocycling in the macronuclear anlagen of Tetrahymena. Eukaryotic cell 9(9):1343-53
  2. Ref:16712831: Li S, Yin L, Cole ES, Udani RA, Karrer KM (2006) Progeny of germ line knockouts of ASI2, a gene encoding a putative signal transduction receptor in Tetrahymena thermophila, fail to make the transition from sexual reproduction to vegetative growth. Developmental biology 295(2):633-46

Sequences 

>TTHERM_00191480(coding)
ATGACACAAACGGAACTCGTTCCTGAAGATTATGACCAGGATAATTAAGATGCTTCTCAA
AGCGTAATCTCTTCAGGAAGCGTTGAAGATGGAAAAGAAAAATTTAAAGCAATGATACAT
AAAATTTTAGGAGTAAACGATAACAAATAAATAGATAAAATATGCTGTTTCTCCATTTTT
AATAAGCAGTGGTCTCGTACAAAGCAACTGTTTTGTGTTTACTCAGTCTTTTTGACATTA
TTTATGACTATAATATTAGGGTGTTTTGTGTTCTACTTAAAATTTTGTCCCAATTTCGTA
GGAGATACAATCTTAGAGTAGATTGATAACTCAATTTCTGCAAGCTTTTAAAATTCCTTA
CTTGAATACAGTAAATAAGTACATTAATATTTCAAAGCATATAGTTCTTGGATGATTATG
TTCTCCCAAATAGTAGCTAACAATGTTGGTTGGAATAATTCACTATATTAATCCTCAAAC
GAATTAAATTTATAACCACAACAAAAATATTTTCCTATTTATTAGCCTATCCCATAAAAT
ATAGTAAATAAGTAATATTCTTAGAAAAAGCCCTATTATGATAAAGATATATCATTTTAA
ACTTTTTCTTATTTTCACAACCATCCTGACGATGCAACTACTATTTCACCAGCTACTTAA
ACATATTTGGACTCTGTAGACTTGATTTCTTATTTTTTTTCTAATTTTATATAAGATCCT
AATTTAACTTATGTCTAGTCTTTATATATTTACCTTTAAGATCGAGATGTCATTTGTTTT
TTTCCAGCAGGCCTCATTAAAGTCCCAAAAGATTTTAAGATAGATATGAAATAAAGGCCT
TGGTATTTAAATACTGCTTAAACATTTTAAGCCAATGGAAATAAACCTGTATTTACAATT
ACCAGTCCCTATCCTGATTTAACTACTAAATAAATAACTATAACATACTCCCTTGCAATC
GTCAAACAGTCAGATCCTACTTAAATTGCTGGAGTTATTAATGTAGATGTTATTGTTGGT
AATTTTAGATCTGTTTATGACTTGCTGCAGGAAAAAGATGGTGAATTTTAAATTGCCTTA
GTAGATTATAATACAGAATACATCATTAATGATTGCTTTAATTAAAGTGGAATTTCACAT
TTTTATGATACGAATAATACAAACTTTACACTTGAAAATTGGAATGAAATCAAAGATTTT
AATTAACACCAATAAAGTTAAACATTCATCATTACTAATAGTTAGAATGTAAGATATTAT
TTTGCTCGTACTTTAATAAAAGATACAGTAAATCAAACATTAAATAGATTTTATCTTATC
GTCTTTGGTAAAGAAAGCAATTATCTCTCCTATAGAAACAATATTTAAGATAGAATATAT
AATGAGCTCTATAACTTTTGCATATCATTAATAATTGCTTTTTCTATTTTGTGGTTCTTT
TCATATTTCTATATCTATGCTCTATCACGCAATATTGTTCTACCGCTTTTTAAATTGAGT
TAATTAGCTAAAAGTATAAACAAGCACGATGAGAGAAAAAGCTCTGCTGAAGACTTGAAA
GAATAGTTAGGTAAGATTTAAGGTGCAGATACTGATACAAAAAAGCTTATCGATTCTTTC
ATTAACCTTGTAGATTAGCACTCTGGCATGAGAAAACTAGCAAATGGTAATGGTTACTAA
GACAATATGATCCCAGTCTACAATAGAATGAGCAACATTTATGAAAACTATTCTAGGAAT
GACTACAGAGAAGAATTGGATGATATATAAGAATAAATTAAATAATTAAAGAATTTAGAT
TAGGAAGATGAAGATGATTATGATGAAAATGGATCTAATTAAGATATTGATGAAGTCTAT
GCTGGAAATCCTAAAAATAAATCTTATTCATCAATTTAATCTAATAAATATTAAAACGAA
GATGATTAAACAGAAGCTGATCTTAGAAATCATAAAAAAAAGTTAGAGTTAGAAGCGAAA
GGAGGCAACCGTACTTCAACAAATTCTTTAGCTAGTAATTTAGAAAACTATAATAAAAGA
AAATATCTAAATTGGATTTAAACTTAACTCTAAGTAATACAAGAAAACGAATAAGCTAAA
ATAAAGTTGTCTCAATAAATTTTATAGTAATTTTTACCTACAAATACTTAATCTATTTGA


>TTHERM_00191480(gene)
ATGACACAAACGGAACTCGTTCCTGAAGATTATGACCAGGATAATTAAGATGCTTCTCAA
AGCGTAATCTCTTCAGGAAGCGTTGAAGATGGAAAAGAAAAATTTAAAGCAATGATACAT
AAAATTTTAGGAGTAAACGATAACAAATAAATAGATAAAATATGCTGTTTCTCCATTTTT
AATAAGCAGTGGTCTCGTACAAAGCAACTGTTTTGTGTTTACTCAGTCTTTTTGACATTA
TTTATGACTATAATATTAGGGTGTTTTGTGTTCTACTTAAAATTTTGTCCCAATTTCGTA
GGAGATACAATCTTAGAGTAGATTGATAACTCAATTTCTGCAAGCTTTTAAAATTCCTTA
CTTGAATACAGTAAATAAGTACATTAATATTTCAAAGCATATAGTTCTTGGATGATTATG
TTCTCCCAAATAGTAGCTAACAATGTTGGTTGGAATAATTCACTATATTAATCCTCAAAC
GAATTAAATTTATAACCACAACAAAAATATTTTCCTATTTATTAGCCTATCCCATAAAAT
ATAGTAAATAAGTAATATTCTTAGAAAAAGCCCTATTATGATAAAGATATATCATTTTAA
ACTTTTTCTTATTTTCACAACCATCCTGACGATGCAACTACTATTTCACCAGCTACTTAA
ACATATTTGGACTCTGTAGACTTGATTTCTTATTTTTTTTCTAATTTTATATAAGATCCT
AATTTAACTTATGTCTAGTCTTTATATATTTACCTTTAAGATCGAGATGTCATTTGTTTT
TTTCCAGCAGGCCTCATTAAAGTCCCAAAAGATTTTAAGATAGATATGAAATAAAGGCCT
TGGTATTTAAATACTGCTTAAACATTTTAAGCCAATGGAAATAAACCTGTATTTACAATT
ACCAGTCCCTATCCTGATTTAACTACTAAATAAATAACTATAACATACTCCCTTGCAATC
GTCAAACAGGTAATTCATAAATAAATCAATATTTCTATTATTTTTAATTTTGACAATTTA
AAATGCAAATAACTAAATAATAATATTTATTTATTTATTTATTTGCTTTTTGATTTTTCA
GTGGGTTAATTAATTTAAAATTTTTAATTAAAATAGTCAGATCCTACTTAAATTGCTGGA
GTTATTAATGTAGATGTTATTGTTGGTAATTTTAGATCTGTTTATGACTTGCTGCAGGAA
AAAGATGGTGAATTTTAAATTGCCTTAGTAGATTATAATACAGAATACATCATTAATGAT
TGCTTTAATTAAAGTGGAATTTCACATTTTTATGATACGAATAATACAAACTTTACACTT
GAAAATTGGAATGAAATCAAAGATTTTAATTAACACCAATAAAGTTAAACATTCATCATT
ACTAATAGTTAGAATGTAAGATATTATTTTGCTCGTACTTTAATAAAAGATACAGTAAAT
CAAACATTAAATAGATTTTATCTTATCGTCTTTGGTAAAGAAAGCAATTATCTCTCCTAT
AGAAACAATATTTAAGATAGAATATATAATGAGCTCTATAACTTTTGCATATCATTAATA
ATTGCTTTTTCTATTTTGTGGTTCTTTTCATATTTCTATATCTATGCTCTATCACGCAAT
ATTGTTCTACCGCTTTTTAAATTGAGTTAATTAGCTAAAAGTATAAACAAGCACGATGAG
AGAAAAAGCTCTGCTGAAGACTTGAAAGAATAGTTAGGTAAGATTTAAGGTGCAGATACT
GATACAAAAAAGCTTATCGATTCTTTCATTAACCTTGTAGATTAGCACTCTGGCATGAGA
AAACTAGCAAATGGTAATGGTTACTAAGACAATATGATCCCAGTCTACAATAGAATGAGC
AACATTTATGAAAACTATTCTAGGAATGACTACAGAGAAGAATTGGATGATATATAAGAA
TAAATTAAATAATTAAAGAATTTAGATTAGGAAGATGAAGATGATTATGATGAAAATGGA
TCTAATTAAGATATTGATGAAGTCTATGCTGGAAATCCTAAAAATAAATCTTATTCATCA
ATTTAATCTAATAAATATTAAAACGAAGATGATTAAACAGAAGCTGATCTTAGAAATCAT
AAAAAAAAGTTAGAGTTAGAAGCGAAAGGAGGCAACCGTACTTCAACAAATTCTTTAGCT
AGTAATTTAGAAAACTATAATAAAAGAAAATATCTAAATTGGATTTAAACTTAACTCTAA
GTAATACAAGAAAACGAATAAGCTAAAATAAAGTTGTCTCAATAAATTTTATAGTAATTT
TTACCTACAAATACTTAATCTATTTGA


>TTHERM_00191480(protein)
MTQTELVPEDYDQDNQDASQSVISSGSVEDGKEKFKAMIHKILGVNDNKQIDKICCFSIF
NKQWSRTKQLFCVYSVFLTLFMTIILGCFVFYLKFCPNFVGDTILEQIDNSISASFQNSL
LEYSKQVHQYFKAYSSWMIMFSQIVANNVGWNNSLYQSSNELNLQPQQKYFPIYQPIPQN
IVNKQYSQKKPYYDKDISFQTFSYFHNHPDDATTISPATQTYLDSVDLISYFFSNFIQDP
NLTYVQSLYIYLQDRDVICFFPAGLIKVPKDFKIDMKQRPWYLNTAQTFQANGNKPVFTI
TSPYPDLTTKQITITYSLAIVKQSDPTQIAGVINVDVIVGNFRSVYDLLQEKDGEFQIAL
VDYNTEYIINDCFNQSGISHFYDTNNTNFTLENWNEIKDFNQHQQSQTFIITNSQNVRYY
FARTLIKDTVNQTLNRFYLIVFGKESNYLSYRNNIQDRIYNELYNFCISLIIAFSILWFF
SYFYIYALSRNIVLPLFKLSQLAKSINKHDERKSSAEDLKEQLGKIQGADTDTKKLIDSF
INLVDQHSGMRKLANGNGYQDNMIPVYNRMSNIYENYSRNDYREELDDIQEQIKQLKNLD
QEDEDDYDENGSNQDIDEVYAGNPKNKSYSSIQSNKYQNEDDQTEADLRNHKKKLELEAK
GGNRTSTNSLASNLENYNKRKYLNWIQTQLQVIQENEQAKIKLSQQILQQFLPTNTQSI