Identifiers and Description

Gene Model Identifier

TTHERM_00193849

Standard Name

RPB3 (RNA Polymerase II subunit 3)

Aliases

cnjC | 16.m05348

Description

DNA-directed rna polymerase II subunit; RPB3 conjugation stage-specific protein

Genome Browser (Macronucleus)

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Genome Browser (Micronucleus)

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External Links

Gene Ontology Annotations

Molecular Function

Biological Process

Domains

  • ( PF00037 ) 4Fe-4S binding domain
  • ( PF01000 ) RNA polymerase Rpb3/RpoA insert domain
  • ( PF01193 ) RNA polymerase Rpb3/Rpb11 dimerisation domain

Gene Expression Profile

Vegetative Cell Cycle (Zhang et al., 2023)

GeneMania

Tetrahymena Stock Center

  • ( SD01817 ) Macronucleus: Complete replacement of RPB3 with c-terminal HA tag and neo3 in 3’ flanking region
  • ( SD01935 ) Micronucleus: RPB3 coding replaced by GFP sequence, neo3 in 3’ flanking region. Heterozygous
  • ( SD01936 ) Micronucleus: RPB3 coding replaced by GFP sequence, neo3 in 3’ flanking region. Heterozygous
  • ( SD02653 ) Micronucleus: GFP replaces RPB3 coding, neo3 in 3’flanking region. homozygous
  • ( SD02654 ) Micronucleus: GFP replaces RPB3 coding, neo3 in 3’ flanking region. homozygous

Homologs

Source Identifier Score
Tetrahymena borealis EI9_03659.1 9.997093137033517e-262
Description: DNA-directed RNA polymerase II subunit (311 aa)
WormBase WBGene00006830 9.997093137033517e-262
Description: locus:unc-103 status:Partially_confirmed UniProt:G5EFT6 protein_id:CAO78715.1
DictyBase DDB_G0292244 9.997863464222902e-69
Description: rpb3 on chromosome: 6 position 1368967 to 1370237
Oxytricha Contig8173.0.g80 8.99739772315193e-58
Description: RNA polymerase Rpb3/RpoA insert domain
SGD YIL021W 2.00017271388466e-38
Description: RPB3 RNA polymerase II third largest subunit B44, part of central core; similar to prokaryotic alpha subunit
WormBase WBGene00007971 3.9997566980151843e-38
Description: locus:rpb-3 status:Partially_confirmed UniProt:Q93338 protein_id:CAB02268.2
Tetrahymena borealis EI9_10244.1 3.9997566980151843e-38
Description: hypothetical protein (513 aa)
Stentor Coeruleus SteCoe_22357 3.975449735908647e-31
Description: None

General Information

No Data fetched for General Information

Associated Literature

  1. Ref:38197489: Shehzada S, Noto T, Saksouk J, Mochizuki K (2024) A SUMO E3 ligase promotes long non-coding RNA transcription to regulate small RNA-directed DNA elimination. eLife 13:
  2. Ref:15470252: Mochizuki K, Gorovsky MA (2004) RNA polymerase II localizes in Tetrahymena thermophila meiotic micronuclei when micronuclear transcription associated with genome rearrangement occurs. Eukaryotic cell 3(5):1233-40
  3. Ref:10704239: Larsen LK, Andreasen PH, Dreisig H, Palm L, Nielsen H, Engberg J, Kristiansen K (1999) Cloning and characterization of the gene encoding the highly expressed ribosomal protein l3 of the ciliated protozoan Tetrahymena thermophila. Evidence for differential codon usage in highly expressed genes. Cell biology international 23(8):551-60
  4. Ref:2112240: Martindale DW (1990) A conjugation-specific gene (cnjC) from Tetrahymena encodes a protein homologous to yeast RNA polymerase subunits (RPB3, RPC40) and similar to a portion of the prokaryotic RNA polymerase alpha subunit (rpoA). Nucleic acids research 18(10):2953-60

Sequences 

>TTHERM_00193849(coding)
ATGGAAGGAATTAAATTAACTCTTACAAACCTCGAAGAATTCAAATGCGAATTTACCCTA
GAAAATTGCGATATCTCATTAGCTAATTCATTAAGAAGAATTCTTTTGGCTGAAATTGAA
ACAATAGCTATCCATAACGTTAAAGTATATGAGAATACATCTTCTCTTCCTGATGAATAC
ATAGCACATAGATTAGGTTTAATTCCTTTAAATTCTGCTAAAGTTGATGATTATAACTAT
ATTTGGAATTGCAATTGCCAAGGTGAATGCGATAGATGTACAATTCATTTTGATTTGAGA
GTGAGTAACTTTACAAATGAAGTCTTAGAAGTTACTTCTCTTGATCTTAAATAAAGATAG
GACCACACTGATGAAGATGCCGTTCGCCCTATAAAGGTATATTCAATTTTACGTGATGAA
TATGGAAATAAGAGAGAAGTAGGTATTCCTATTATCAAGCTATCTAAAGGACAATAAATT
CACTTTGAATGTGAAGCCTAGAAAGGTATCGGTAAAATGCATTCTAAATGGAATCCAGTA
TGTATTTCAACTTTCTCAGTTGAACCTGAAATTATTTTCGATCAAGAAATTTAAAATTTA
GATATTGAATAGAAGAAAGCTCTAGTAAATGCATGTCCTACTAAAGTATTTGGTATCAAT
CCTCATACCAATACTTTGGAAGTCTAAGATCACATGAAATGTATGTATTGTGAAGAATGT
GTTTACAAGTGTGCTGAAGACTTCTAAAAACCTAAATTAATCAAAATAGACCATAAAAAG
GATAAGTTTTTCTTCAAAGTCGAAACTACAGGAGTTATGAAGCCTGTTGAAGTGTTAAGA
AGAGGTTTTTCAACCTTGAGATAGAAGCTTGATTTTATGAGAGAATAAATCTAAGAAAAT
TCTAATAATTAATTTGAATATGGTAACGAGTGA


>TTHERM_00193849(gene)
ATGGAAGGAATTAAATTAACTCTTACAAACCTCGAAGAATTCAAATGCGAATTTACCCTA
GAAAATTGCGATATCTCATTAGCTAATTCATTAAGAAGAATTCTTTTGGCTGAAATTGAA
ACAATAGCTATCCATAACGTTAAAGTATATGAGAATACATCTTCTCTTCCTGATGAATAC
ATAGCACATAGATTAGGTTTAATTCCTTTAAATTCTGCTAAAGTTGATGATTATAACTAT
ATTTGGAATTGCAATTGCCAAGGTGAATGCGATAGATGTACAATTCATTTTGATTTGAGA
GTGAGTAACTTTACAAATGAAGTCTTAGAAGTTACTTCTCTTGATCTTAAATAAAGATAG
GACCACACTGATGAAGATGCCGTTCGCCCTATAAAGGTATATTCAATTTTACGTGATGAA
TATGGAAATAAGAGAGAAGTAGGTATTCCTATTATCAAGCTATCTAAAGGACAATAAATT
CACTTTGAATGTGAAGCCTAGAAAGGTATCGGTAAAATGCATTCTAAATGGAATCCAGTA
TGTATTTCAACTTTCTCAGTTGAACCTGAAATTATTTTCGATCAAGAAATTTAAAATTTA
GATATTGAATAGAAGAAAGCTCTAGTAAATGCATGTCCTACTAAAGTATTTGGTATCAAT
CCTCATACCAATACTTTGGAAGTCTAAGATCACATGAAATGTATGTATTGTGAAGAATGT
GTTTACAAGTGTGCTGAAGACTTCTAAAAACCTAAATTAATCAAAATAGACCATAAAAAG
GATAAGTTTTTCTTCAAAGTCGAAACTACAGGAGTTATGAAGCCTGTTGAAGTGTTAAGA
AGAGGTTTTTCAACCTTGAGATAGAAGCTTGATTTTATGAGAGAATAAATCTAAGAAAAT
TCTAATAATTAATTTGAATATGGTAACGAGTGA


>TTHERM_00193849(protein)
MEGIKLTLTNLEEFKCEFTLENCDISLANSLRRILLAEIETIAIHNVKVYENTSSLPDEY
IAHRLGLIPLNSAKVDDYNYIWNCNCQGECDRCTIHFDLRVSNFTNEVLEVTSLDLKQRQ
DHTDEDAVRPIKVYSILRDEYGNKREVGIPIIKLSKGQQIHFECEAQKGIGKMHSKWNPV
CISTFSVEPEIIFDQEIQNLDIEQKKALVNACPTKVFGINPHTNTLEVQDHMKCMYCEEC
VYKCAEDFQKPKLIKIDHKKDKFFFKVETTGVMKPVEVLRRGFSTLRQKLDFMREQIQEN
SNNQFEYGNE