Identifiers and Description

Gene Model Identifier

TTHERM_00193849

Standard Name

RPB3 (RNA Polymerase II subunit 3)

Aliases

cnjC | 16.m05348

Description

RPB3 DNA-directed rna polymerase II subunit; RPB3 conjugation stage-specific protein; Archaeal Rpo3/Eukaryotic RPB3 RNA Polymerase Subunit

Genome Browser (Macronucleus)

Full Screen View


Genome Browser (Micronucleus)

Full Screen View

External Links

Gene Ontology Annotations

No Data fetched for Gene Ontology Annotations

Domains

  • ( PF01000 ) RNA polymerase Rpb3/RpoA insert domain
  • ( PF01193 ) RNA polymerase Rpb3/Rpb11 dimerisation domain

Gene Expression Profile

Vegetative Cell Cycle (Zhang et al., 2023)

GeneMania

Tetrahymena Stock Center

  • ( SD01817 ) Macronucleus: Complete replacement of RPB3 with c-terminal HA tag and neo3 in 3’ flanking region
  • ( SD01935 ) Micronucleus: RPB3 coding replaced by GFP sequence, neo3 in 3’ flanking region. Heterozygous
  • ( SD01936 ) Micronucleus: RPB3 coding replaced by GFP sequence, neo3 in 3’ flanking region. Heterozygous
  • ( SD02653 ) Micronucleus: GFP replaces RPB3 coding, neo3 in 3’flanking region. homozygous
  • ( SD02654 ) Micronucleus: GFP replaces RPB3 coding, neo3 in 3’ flanking region. homozygous

Homologs

No Data fetched for Homologs

General Information

No Data fetched for General Information

Associated Literature

  1. Ref:38197489: Shehzada S, Noto T, Saksouk J, Mochizuki K (2024) A SUMO E3 ligase promotes long non-coding RNA transcription to regulate small RNA-directed DNA elimination. eLife 13:
  2. Ref:15470252: Mochizuki K, Gorovsky MA (2004) RNA polymerase II localizes in Tetrahymena thermophila meiotic micronuclei when micronuclear transcription associated with genome rearrangement occurs. Eukaryotic cell 3(5):1233-40
  3. Ref:10704239: Larsen LK, Andreasen PH, Dreisig H, Palm L, Nielsen H, Engberg J, Kristiansen K (1999) Cloning and characterization of the gene encoding the highly expressed ribosomal protein l3 of the ciliated protozoan Tetrahymena thermophila. Evidence for differential codon usage in highly expressed genes. Cell biology international 23(8):551-60
  4. Ref:2112240: Martindale DW (1990) A conjugation-specific gene (cnjC) from Tetrahymena encodes a protein homologous to yeast RNA polymerase subunits (RPB3, RPC40) and similar to a portion of the prokaryotic RNA polymerase alpha subunit (rpoA). Nucleic acids research 18(10):2953-60

Sequences 

>TTHERM_00193849(coding)
ATGATAGCTTAAAAGAAAGAGAGCTCTGTCTGCTACTTAATAAGATATATTTATTTACTA
ATATAATTATCTTTATTATCAATTAATTAAATCGCGCGTCTATTGAATATTTTTCTTATA
AAACAAATCTTATTTAAAATTTCTTTTAATTATAAGCAATAATTAAAGTAAACCTTTCAT
AAGAAAAGCTAGCAAAAAAGAGAGACATTTTCTAATTTAAAAACAGGAAATATCAAAATA
AATATAACAACTCAAAAAGTTTAAAGGTCAATTATTAAGTAAATGGAAGGAATTAAATTA
ACTCTTACAAACCTCGAAGAATTCAAATGCGAATTTACCCTAGAAAATTGCGATATCTCA
TTAGCTAATTCATTAAGAAGAATTCTTTTGGCTGAAATTGAAACAATAGCTATCCATAAC
GTTAAAGTATATGAGAATACATCTTCTCTTCCTGATGAATACATAGCACATAGATTAGGT
TTAATTCCTTTAAATTCTGCTAAAGTTGATGATTATAACTATATTTGGAATTGCAATTGC
CAAGGTGAATGCGATAGATGTACAATTCATTTTGATTTGAGAGTGAGTAACTTTACAAAT
GAAGTCTTAGAAGTTACTTCTCTTGATCTTAAATAAAGATAGGACCACACTGATGAAGAT
GCCGTTCGCCCTATAAAGGTATATTCAATTTTACGTGATGAATATGGAAATAAGAGAGAA
GTAGGTATTCCTATTATCAAGCTATCTAAAGGACAATAAATTCACTTTGAATGTGAAGCC
TAGAAAGGTATCGGTAAAATGCATTCTAAATGGAATCCAGTATGTATTTCAACTTTCTCA
GTTGAACCTGAAATTATTTTCGATCAAGAAATTTAAAATTTAGATATTGAATAGAAGAAA
GCTCTAGTAAATGCATGTCCTACTAAAGTATTTGGTATCAATCCTCATACCAATACTTTG
GAAGTCTAAGATCACATGAAATGTATGTATTGTGAAGAATGTGTTTACAAGTGTGCTGAA
GACTTCTAAAAACCTAAATTAATCAAAATAGACCATAAAAAGGATAAGTTTTTCTTCAAA
GTCGAAACTACAGGAGTTATGAAGCCTGTTGAAGTGTTAAGAAGAGGTTTTTCAACCTTG
AGATAGAAGCTTGATTTTATGAGAGAATAAATCTAAGAAAATTCTAATAATTAATTTGAA
TATGGTAACGAGTGA



>TTHERM_00193849(gene)
AAAACAATGATAGCTTAAAAGAAAGAGAGCTCTGTCTGCTACTTAATAAGATATATTTAT
TTACTAATATAATTATCTTTATTATCAATTAATTAAATCGCGCGTCTATTGAATATTTTT
CTTATAAAACAAATCTTATTTAAAATTTCTTTTAATTATAAGCAATAATTAAAGTAAACC
TTTCATAAGAAAAGCTAGCAAAAAAGAGAGACATTTTCTAATTTAAAAACAGGAAATATC
AAAATAAATATAACAACTCAAAAAGTTTAAAGGTCAATTATTAAGTAAATGGAAGGAATT
AAATTAACTCTTACAAACCTCGAAGAATTCAAATGCGAATTTACCCTAGAAAATTGCGAT
ATCTCATTAGCTAATTCATTAAGAAGAATTCTTTTGGCTGAAATTGAAACAATAGCTATC
CATAACGTTAAAGTATATGAGAATACATCTTCTCTTCCTGATGAATACATAGCACATAGA
TTAGGTTTAATTCCTTTAAATTCTGCTAAAGTTGATGATTATAACTATATTTGGAATTGC
AATTGCCAAGGTGAATGCGATAGATGTACAATTCATTTTGATTTGAGAGTGAGTAACTTT
ACAAATGAAGTCTTAGAAGTTACTTCTCTTGATCTTAAATAAAGATAGGACCACACTGAT
GAAGATGCCGTTCGCCCTATAAAGGTATATTCAATTTTACGTGATGAATATGGAAATAAG
AGAGAAGTAGGTATTCCTATTATCAAGCTATCTAAAGGACAATAAATTCACTTTGAATGT
GAAGCCTAGAAAGGTATCGGTAAAATGCATTCTAAATGGAATCCAGTATGTATTTCAACT
TTCTCAGTTGAACCTGAAATTATTTTCGATCAAGAAATTTAAAATTTAGATATTGAATAG
AAGAAAGCTCTAGTAAATGCATGTCCTACTAAAGTATTTGGTATCAATCCTCATACCAAT
ACTTTGGAAGTCTAAGATCACATGAAATGTATGTATTGTGAAGAATGTGTTTACAAGTGT
GCTGAAGACTTCTAAAAACCTAAATTAATCAAAATAGACCATAAAAAGGATAAGTTTTTC
TTCAAAGTCGAAACTACAGGAGTTATGAAGCCTGTTGAAGTGTTAAGAAGAGGTTTTTCA
ACCTTGAGATAGAAGCTTGATTTTATGAGAGAATAAATCTAAGAAAATTCTAATAATTAA
TTTGAATATGGTAACGAGTGAGCTATTTACAGTCAAGTTTCTAGCTTAAAAATAGTTTTT
TTTATTTTAAACTGGCTTTATAAAATTATTTATATTAAAAATGTCTTATCTTTGTGGTCA
AAAAGTATGCATTTATCAATCTAAATATTTTATAAGTTAATTTTTTAGCTTGATGGATAA
TTGTTC



>TTHERM_00193849(protein)
MIAQKKESSVCYLIRYIYLLIQLSLLSINQIARLLNIFLIKQILFKISFNYKQQLKQTFH
KKSQQKRETFSNLKTGNIKINITTQKVQRSIIKQMEGIKLTLTNLEEFKCEFTLENCDIS
LANSLRRILLAEIETIAIHNVKVYENTSSLPDEYIAHRLGLIPLNSAKVDDYNYIWNCNC
QGECDRCTIHFDLRVSNFTNEVLEVTSLDLKQRQDHTDEDAVRPIKVYSILRDEYGNKRE
VGIPIIKLSKGQQIHFECEAQKGIGKMHSKWNPVCISTFSVEPEIIFDQEIQNLDIEQKK
ALVNACPTKVFGINPHTNTLEVQDHMKCMYCEECVYKCAEDFQKPKLIKIDHKKDKFFFK
VETTGVMKPVEVLRRGFSTLRQKLDFMREQIQENSNNQFEYGNE