Identifiers and Description

Gene Model Identifier

TTHERM_00283330

Standard Name

CET1

Aliases

p138 | PreTt04872 | 25.m00336 | 3694.m00119 | Spt16

Description

CET1 metallopeptidase family M24 containing protein; Protein associated with macronulear microtubules; sequence similarity to human transcription elongation factor FACT 140 kDa subunit, yeast Spt16/Cdc68, and Xenopus DUF140; FACT complex subunit Spt16

Genome Browser (Macronucleus)

Full Screen View

No Genome Browser Data Present

Genome Browser (Micronucleus)

Full Screen View

No Genome Browser Data Present

External Links

• Uniprot
• Alphafold

Gene Ontology Annotations

Cellular Component

• FACT complex (ISS) | GO:0035101
• macronucleus (IDA) | GO:0031039

Molecular Function

• chromatin binding (IDA) | GO:0003682
• microtubule binding (IDA) | GO:0008017
• protein heterodimerization activity (ISS) | GO:0046982

Domains

• ( PF00557 ) Metallopeptidase family M24
• ( PF08512 ) Histone chaperone Rttp106-like, middle domain
• ( PF08644 ) FACT complex subunit (SPT16/CDC68)
• ( PF14826 ) FACT complex subunit SPT16 N-terminal lobe domain

Gene Expression Profile

Vegetative Cell Cycle (Zhang et al., 2023)

GeneMania

No results were calculated for this gene in GeneMania.

Tetrahymena Stock Center

No Data fetched for Tetrahymena Stock Center

Homologs

No Data fetched for Homologs

General Information

No.	Gene Name(s)	Paragraph Text
2334	CET1	The FACT-complex is a critical transcription regulator and an H2A/H2B chaperone. Affinity purification followed by mass spectrometry (AP-MS) of endogenously tagged T. thermophila histones H2A/H2B identified Cet1 and Pob3 subunits of the FACT complex as high-confidence interacting partners. Indirect immunofluorescence studies using endogenously tagged Cet1 indicated that it localized to both the MAC and MIC during vegetative growth. AP-MS analysis using Tetrahymena Cet1 as a bait protein revealed Pob3 and subunits of RNA polymerase as high-confidence co-purifying proteins (Ashraf et al. 2019).

Associated Literature

1. Ref:34086947: Nabeel-Shah S, Garg J, Saettone A, Ashraf K, Lee H, Wahab S, Ahmed N, Fine J, Derynck J, Pu S, Ponce M, Marcon E, Zhang Z, Greenblatt JF, Pearlman RE, Lambert JP, Fillingham J (2021) Functional characterization of RebL1 highlights the evolutionary conservation of oncogenic activities of the RBBP4/7 orthologue in Tetrahymena thermophila. Nucleic acids research ( ):
2. Ref:30796450: Ashraf K, Nabeel-Shah S, Garg J, Saettone A, Derynck J, Gingras AC, Lambert JP, Pearlman RE, Fillingham J (2019) Proteomic analysis of histones H2A/H2B and variant Hv1 in Tetrahymena thermophila reveals an ancient network of chaperones. Molecular biology and evolution ( ):
3. Ref:15013445: Fujiu K, Numata O (2004) Identification and molecular cloning of Tetrahymena 138-kDa protein, a transcription elongation factor homologue that interacts with microtubules in vitro. Biochemical and biophysical research communications 315(1):196-203

Sequences

>TTHERM_00283330(coding)
ATGAGCAAAGTTAAAGCTGATATCTTTTATCAACACCATAAGCAGCTATTGAGTGTTTGG
AATAAGCAACCTCAATACGCCAATATCGATGCTTTTATTATTAAAAATGGTAAAGATCAA
GGTGGAAATAAAATCAAAACTTCTGCCATTTCTATGTGGTATTTTGGTTTCGACTTCATT
GACACTATCCTTTTGATCACTAAAAAGACTTTTGCCATTATTGGTGGTAACAAGAAAATA
AACATGTTAAAATCTGTCTAGGAACACGCAGAAGCAAAAGAATACAATTTAGTTTTCATC
GAAAAAGACTAAGCAAACAATTCCAATCAACTTTAATAATTATTTGAAATTCTTGACAAA
GATTTGAACAAATCTAGCTTCAATATTGGAACTCTAGCTAAAGAATAGCAAGTTGGCCCT
TTTATGACTGAGTATGACTCATTTATTAAAGACAAAAATTAATATAAATTTGCTGATTGC
AGTGTATTTGTATAAGATTGTCTCTCTGTAAAAGATCAAAATGAAATATCTTACATTGGA
AAAGCTGCTAAAGTTAGTGTCTATTTAGAGTCTAAATTGATAAAAGAAATTGAAACAATC
ATTGAAGATGAAGGTAAAAAGACTCACAGTCAAATTGCTACTATGATTGAAGGATTGATT
GAAAATGAAAAAGAACTTAAAAAGATCTCAGAAGAAATTGGAGGTGAGAGTGACAATCTT
GATTTGGCTTATGTCCCAATTGTCTAATCTGGCGGCAAATATGATTTAAAACCAAATGCT
TAATCTAATGAAGATATTCTTTCTTATGACACAATTATTGTTTCAGTAGGTACTAAATAT
ATGGAATATCATGCTAATATTGTTCGTACCCTTTTCATTGACCCAACAAACGACTAAAAG
AAGATTTATTAGAGAGTTTATGAGCTCTAAAACTAGATCGCTGTCTAACTTAAGCCTGGT
ATTAAGCTCAAAACTGTTTATGAAAATGCAGTTAATTTCATTAATGAGAAAGTACCTCAA
TTGAAGGATAAGATCCCAGCTAATTTTGGATTTGGTATTGGTTTAGAATTTAGAGAATCT
AACTTGTACATTAATGCTAAAAACGAAAAAGAAGTTGAAGAAGGTATGGTCTTCAATGTT
GTTGTTGGTTTTGACAACTTGCAAAGTGAAAAGGAGAAAGCTTATGCCATACAAATTTCT
GACACTGTTGCAATTAGAAAGTAAAACACACCCAATGCTGTCATGACATTCAAAGTTTCT
AAGAAATATGAAGACATTTCTTACAGCATTTAAGATGAGGGTCAAGATGAAGAATAAGAA
GAGGAAGAAGATGATTTAGAAAAGGAAAACATTATTTAAGATGGACGCAGAACAAGAAAT
GCCTATCACAAAAATACAACTATTGTTTCAGAAAAAGAAAGACAAAAACACTAACTTGAA
TTGAGAGAAGTCAAATTAAAAGAATTACAAGAAAGATATAATAACAATGGATTCTTATCA
AATAAGATCAATTCCAGAGCTCTTGAGTTAGATAAGGTCTAGTGTTATGGTGGACCTCAA
GATATTCCCAAAGAATATAAGAAAAATTAAATTCATATTGATGCTGCTCATAATGCAATC
TTACTTCCTGTAAATGGTGAACTAGTTCCCTTCCACATTTCCTTAATTAAAAACTACTCT
AAAAACGACGAAGGAAAGACTCACACATTACGTCTTAACTTCCACAATCCTGGTAGTGGC
AGCAATTTAGCCAATATTACTTTCCCTAAGATTGATGGCTAAATTGTCTTTATTAAAGAA
CTCACTTTCCGTTCAAAGAACGCTAAAAATATGTTGGAAACAATTAAGAAAATCAAAGAC
TTGCAAGCTAAAGTTAAACAAACTGATCAAGAAGCTAAAAATAAAGACGAATTAGTAGAG
CAAGACAAATTACAATTACGTAACACAAAGAGACCTGCTTTAAGAAATTTAAAAGTACGT
CCTGCTATTTCTAAATAAAAAGTAAATGGTATGTTGGAATTGCACTTAAATGGTTTCAGA
TATATGACAACTAAAAATGAAAAAGTTGATGTCATCTTTAAAAATATAAAACATGCCATC
TTCTAACCTTGCGATAACGAAATGATTGTTGCCATACATTTCAATCTCAAGAATCCTATT
ATGATAGGCAAAAAGAAAGTTTGGGATGTACAATTTTATACAGAAGCTGGTCTTCCCCCA
GAGGATTTAAATAACAGAAGAAGAGGTCATGACTACGATGAAATAGAAGAAGAATAAATG
GAAAAAGCTCGTAGAAAGAAATTAAATAAGGACTTCGAAGCCTTCTACAAAGAAGTTGAA
AACTAGCTTGGAGATAAAATTAAGTTTGAAGTTCCATATGCCAATCTCGGTTTCTACGGT
TCACCAAGTAGATCTACTTGCCTCCTTTAACCTACATAAAATACCCTTATGAACATCATT
GAATTCCCCTTCTTTATCATGTCCTTAGAAGAAGTAGAATTAGCATGCTTCGAAAGAATG
ATCGGAAGACTTAAAAACTTTGATCTTGTCTTTATTTTCAAGGATTATGAAAAACAAGTT
ACAAGAATTGCCTCTATTCCTATTGATAAGGCAGAAATAGTTAAAAATTGGTTAAACAGC
TAAAATATATTGTATTTTGAATCTACAAAGAGTTTCTCTTGGGCTAATATCTTAAAGACC
ATTCGCTAAGATATTGGTGGCTTCATTGAAGATGGAGGTTGGAATATTATTTTGGGTGAA
GATGAAGGAGAAGATGAAGATCCTGATGAGGGTGATGAAGATTTCTCAGTAGAACAAGAC
GAAGAGGAAGAAGAAAGTGAAGAGATTTCTGAAAGTGATGAGGATTTCGATGATGAAGAA
AGTGCGAGTGATTTTGAAGGAGATGAAGATCTCAGTGAAGAAGGCTAAAGCTGGAGCGAT
CTTGAAGAAAACGCTAGAAAGGAAGAAGAGAGAAGAAAGTAACAACAAGTAAAACCAACA
AAGCCTGTTCCTTAAAAAAGAAGATGA

>TTHERM_00283330(gene)
TCAGCGAATAATAAACTAATTAACTAACTAACAAACATAATATTTGCGAAATAAATACAG
ACAGACTTAATTAATAATAAATAGTATATTGATAACACTTTTATATTGTGCAAAAATTAG
CAAGAATCCAATTAAAAATCAAATACAAAGATAAATACTAACTTAAAGATATTCATATTT
ATAATAATATCAAAGCAAATAAACTTTTTGATATATTTTCAGAAAGAAATAAAGAAACAA
ACAACTTGCGTTCTTATCAAGTTTTATAAGGCTCTACAAAGTTAAATTGAAAAAGTAAAT
CTTTCAGAAAAATGAGCAAAGTTAAAGCTGATATCTTTTATCAACACCATAAGCAGCTAT
TGAGTGTTTGGAATAAGCAACCTCAATACGCCAATATCGATGCTTTTATTATTAAAAATG
GTAAAGATCAAGGTGGAAATAAAATCAAAACTTCTGCCATTTCTATGTGGTATTTTGGTT
TCGACTTCATTGACACTATCCTTTTGATCACTAAAAAGACTTTTGCCATTATTGGTGGTA
ACAAGAAAATAAACATGTTAAAATCTGTCTAGGAACACGCAGAAGCAAAAGAATACAATT
TAGTTTTCATCGAAAAAGACTAAGCAAACAATTCCAATCAACTTTAATAATTATTTGAAA
TTCTTGACAAAGATTTGAACAAATCTAGCTTCAATATTGGAACTCTAGCTAAAGAATAGC
AAGTTGGCCCTTTTATGACTGAGTATGACTCATTTATTAAAGACAAAAATTAATATAAAT
TTGCTGATTGCAGTGTATTTGTATAAGATTGTCTCTCTGTAAAAGATCAAAATGAAATAT
CTTACATTGGAAAAGCTGCTAAAGTTAGTGTCTATTTAGAGTCTAAATTGATAAAAGAAA
TTGAAACAATCATTGAAGATGAAGGTAAAAAGACTCACAGTCAAATTGCTACTATGATTG
AAGGATTGATTGAAAATGAAAAAGAACTTAAAAAGATCTCAGAAGAAATTGGAGGTGAGA
GTGACAATCTTGATTTGGCTTATGTCCCAATTGTCTAATCTGGCGGCAAATATGATTTAA
AACCAAATGCTTAATCTAATGAAGATATTCTTTCTTATGACACAATTATTGTTTCAGTAG
GTACTAAATATATGGAATATCATGCTAATATTGTTCGTACCCTTTTCATTGACCCAACAA
ACGACTAAAAGAAGATTTATTAGAGAGTTTATGAGCTCTAAAACTAGATCGCTGTCTAAC
TTAAGCCTGGTATTAAGCTCAAAACTGTTTATGAAAATGCAGTTAATTTCATTAATGAGA
AAGTACCTCAATTGAAGGATAAGATCCCAGCTAATTTTGGATTTGGTATTGGTTTAGAAT
TTAGAGAATCTAACTTGTACATTAATGCTAAAAACGAAAAAGAAGTTGAAGAAGGTATGG
TCTTCAATGTTGTTGTTGGTTTTGACAACTTGCAAAGTGAAAAGGAGAAAGCTTATGCCA
TACAAATTTCTGACACTGTTGCAATTAGAAAGTAAAACACACCCAATGCTGTCATGACAT
TCAAAGTTTCTAAGAAATATGAAGACATTTCTTACAGCATTTAAGATGAGGGTCAAGATG
AAGAATAAGAAGAGGAAGAAGATGATTTAGAAAAGGAAAACATTATTTAAGATGGACGCA
GAACAAGAAATGCCTATCACAAAAATACAACTATTGTTTCAGAAAAAGAAAGACAAAAAC
ACTAACTTGAATTGAGAGAAGTCAAATTAAAAGAATTACAAGAAAGATATAATAACAATG
GATTCTTATCAAATAAGATCAATTCCAGAGCTCTTGAGTTAGATAAGGTCTAGTGTTATG
GTGGACCTCAAGATATTCCCAAAGAATATAAGAAAAATTAAATTCATATTGATGCTGCTC
ATAATGCAATCTTACTTCCTGTAAATGGTGAACTAGTTCCCTTCCACATTTCCTTAATTA
AAAACTACTCTAAAAACGACGAAGGAAAGACTCACACATTACGTCTTAACTTCCACAATC
CTGGTAGTGGCAGCAATTTAGCCAATATTACTTTCCCTAAGATTGATGGCTAAATTGTCT
TTATTAAAGAACTCACTTTCCGTTCAAAGAACGCTAAAAATATGTTGGAAACAATTAAGA
AAATCAAAGACTTGCAAGCTAAAGTTAAACAAACTGATCAAGAAGCTAAAAATAAAGACG
AATTAGTAGAGCAAGACAAATTACAATTACGTAACACAAAGAGACCTGCTTTAAGAAATT
TAAAAGTACGTCCTGCTATTTCTAAATAAAAAGTAAATGGTATGTTGGAATTGCACTTAA
ATGGTTTCAGATATATGACAACTAAAAATGAAAAAGTTGATGTCATCTTTAAAAATATAA
AACATGCCATCTTCTAACCTTGCGATAACGAAATGATTGTTGCCATACATTTCAATCTCA
AGAATCCTATTATGATAGGCAAAAAGAAAGTTTGGGATGTACAATTTTATACAGAAGCTG
GTCTTCCCCCAGAGGATTTAAATAACAGAAGAAGAGGTCATGACTACGATGAAATAGAAG
AAGAATAAATGGAAAAAGCTCGTAGAAAGAAATTAAATAAGGACTTCGAAGCCTTCTACA
AAGAAGTTGAAAACTAGCTTGGAGATAAAATTAAGTTTGAAGTTCCATATGCCAATCTCG
GTTTCTACGGTTCACCAAGTAGATCTACTTGCCTCCTTTAACCTACATAAAATACCCTTA
TGAACATCATTGAATTCCCCTTCTTTATCATGTCCTTAGAAGAAGTAGAATTAGCATGCT
TCGAAAGAATGATCGGAAGACTTAAAAACTTTGATCTTGTCTTTATTTTCAAGGATTATG
AAAAACAAGTTACAAGAATTGCCTCTATTCCTATTGATAAGGCAGAAATAGTTAAAAATT
GGTTAAACAGCTAAAATATATTGTATTTTGAATCTACAAAGAGTTTCTCTTGGGCTAATA
TCTTAAAGACCATTCGCTAAGATATTGGTGGCTTCATTGAAGATGGAGGTTGGAATATTA
TTTTGGGTGAAGATGAAGGAGAAGATGAAGATCCTGATGAGGGTGATGAAGATTTCTCAG
TAGAACAAGACGAAGAGGAAGAAGAAAGTGAAGAGATTTCTGAAAGTGATGAGGATTTCG
ATGATGAAGAAAGTGCGAGTGATTTTGAAGGAGATGAAGATCTCAGTGAAGAAGGCTAAA
GCTGGAGCGATCTTGAAGAAAACGCTAGAAAGGAAGAAGAGAGAAGAAAGTAACAACAAG
TAAAACCAACAAAGCCTGTTCCTTAAAAAAGAAGATGAAAAATAATATATATAATTATTA
AACTCTAGATTTACCAACTAACCTCAATTAATTTTATTCACTTAAGTAAATCTATTGTTT
AATAAATTAAAACTTTGTAAATAAATAAATAAATTTTAGGATATAAAAAAGTAATTAATA
TATGTAACTAAATTCTTCGCATTCTCTTCATCTATCTTAAAAATTCAAAGATTAATTTAT
TTTTCTAAATATTTTTTATGTTTTGCTAAAAATCTATTAATAAATCCTAAGTTGATTT

>TTHERM_00283330(protein)
MSKVKADIFYQHHKQLLSVWNKQPQYANIDAFIIKNGKDQGGNKIKTSAISMWYFGFDFI
DTILLITKKTFAIIGGNKKINMLKSVQEHAEAKEYNLVFIEKDQANNSNQLQQLFEILDK
DLNKSSFNIGTLAKEQQVGPFMTEYDSFIKDKNQYKFADCSVFVQDCLSVKDQNEISYIG
KAAKVSVYLESKLIKEIETIIEDEGKKTHSQIATMIEGLIENEKELKKISEEIGGESDNL
DLAYVPIVQSGGKYDLKPNAQSNEDILSYDTIIVSVGTKYMEYHANIVRTLFIDPTNDQK
KIYQRVYELQNQIAVQLKPGIKLKTVYENAVNFINEKVPQLKDKIPANFGFGIGLEFRES
NLYINAKNEKEVEEGMVFNVVVGFDNLQSEKEKAYAIQISDTVAIRKQNTPNAVMTFKVS
KKYEDISYSIQDEGQDEEQEEEEDDLEKENIIQDGRRTRNAYHKNTTIVSEKERQKHQLE
LREVKLKELQERYNNNGFLSNKINSRALELDKVQCYGGPQDIPKEYKKNQIHIDAAHNAI
LLPVNGELVPFHISLIKNYSKNDEGKTHTLRLNFHNPGSGSNLANITFPKIDGQIVFIKE
LTFRSKNAKNMLETIKKIKDLQAKVKQTDQEAKNKDELVEQDKLQLRNTKRPALRNLKVR
PAISKQKVNGMLELHLNGFRYMTTKNEKVDVIFKNIKHAIFQPCDNEMIVAIHFNLKNPI
MIGKKKVWDVQFYTEAGLPPEDLNNRRRGHDYDEIEEEQMEKARRKKLNKDFEAFYKEVE
NQLGDKIKFEVPYANLGFYGSPSRSTCLLQPTQNTLMNIIEFPFFIMSLEEVELACFERM
IGRLKNFDLVFIFKDYEKQVTRIASIPIDKAEIVKNWLNSQNILYFESTKSFSWANILKT
IRQDIGGFIEDGGWNIILGEDEGEDEDPDEGDEDFSVEQDEEEEESEEISESDEDFDDEE
SASDFEGDEDLSEEGQSWSDLEENARKEEERRKQQQVKPTKPVPQKRR