Identifiers and Description

Gene Model Identifier

TTHERM_00292170

Standard Name

ERF1 (Eukaryotic Release Factor 1)

Aliases

PreTt26050 | 26.m00402 | 3688.m00193

Description

ERF1 eukaryotic peptide chain release factor subunit 1; Eukaryotic release factor; believed to recognize the stop codon of mRNA and terminate translation; gene has 4 introns; deduced protein sequence 57% similar to human eRF1; domain 1 proposed to be the codon recognition site

Genome Browser (Macronucleus)

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Genome Browser (Micronucleus)

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External Links

Gene Ontology Annotations

Cellular Component

Molecular Function

  • translation release factor activity, codon specific (IGI) | GO:0016149
  • translation release factor activity, codon specific (IEA) | GO:0016149

Biological Process

Domains

Gene Expression Profile

Vegetative Cell Cycle (Zhang et al., 2023)

GeneMania

Tetrahymena Stock Center

No Data fetched for Tetrahymena Stock Center

Homologs

Source Identifier Score
Tetrahymena borealis EI9_09405.1 9.997093137033517e-262
Description: eukaryotic peptide chain release factor subunit 1 (436 aa)
Tetrahymena borealis EI9_04878.1 9.997093137033517e-262
Description: hypothetical protein (266 aa)
SGD YBR143C 7.000463540624081e-176
Description: SUP45 Polypeptide release factor (eRF1) in translation termination; mutant form acts as a recessive omnipotent suppressor; methylated by Mtq2p-Trm112p in ternary complex eRF1-eRF3-GTP; mutation of methylation site confers resistance to zymocin
WormBase WBGene00020269 2.998539800509618e-166
Description: locus:erfa-1 status:Confirmed UniProt:S6EZT6 protein_id:CDG24149.1
Tetrahymena borealis EI9_18637.1 2.998539800509618e-166
Description: hypothetical protein (1583 aa)
Oxytricha Contig21968.0.g12 1.0001999920370885e-161
Description: eRF1 domain 2
DictyBase DDB_G0288613 2.0004037638327014e-151
Description: erf1 on chromosome: 5 position 1753244 to 1754781
Stentor Coeruleus SteCoe_8516 5.96629836401057e-72
Description: None

General Information

No Data fetched for General Information

Associated Literature

  1. Ref:16382136: Salas-Marco J, Fan-Minogue H, Kallmeyer AK, Klobutcher LA, Farabaugh PJ, Bedwell DM (2006) Distinct paths to stop codon reassignment by the variant-code organisms Tetrahymena and Euplotes. Molecular and cellular biology 26(2):438-47
  2. Ref:12084909: Ito K, Frolova L, Seit-Nebi A, Karamyshev A, Kisselev L, Nakamura Y (2002) Omnipotent decoding potential resides in eukaryotic translation termination factor eRF1 of variant-code organisms and is modulated by the interactions of amino acid sequences within domain 1. Proceedings of the National Academy of Sciences of the United States of America 99(13):8494-9
  3. Ref:11788716: Inagaki Y, et al. (2002) Convergence and constraint in eukaryotic release factor 1 (eRF1) domain 1: the evolution of stop codon specificity. Nucleic Acids Res 30(2):532-44
  4. Ref:11163755: Muramatsu T, Heckmann K, Kitanaka C, Kuchino Y (2001) Molecular mechanism of stop codon recognition by eRF1: a wobble hypothesis for peptide anticodons. FEBS letters 488(3):105-9
  5. Ref:11762076: Muramatsu T (2001) [Molecular mechanism of stop codon recognition by eRF1: a wobble hypothesis for peptide anticodons]. Tanpakushitsu kakusan koso. Protein, nucleic acid, enzyme 46(15):2163-70
  6. Ref:11231122: Lozupone CA, Knight RD, Landweber LF (2001) The molecular basis of nuclear genetic code change in ciliates. Current biology : CB 11(2):65-74
  7. Ref:11179680: Liang A, Brünen-Nieweler C, Muramatsu T, Kuchino Y, Beier H, Heckmann K (2001) The ciliate Euplotes octocarinatus expresses two polypeptide release factors of the type eRF1. Gene 262(1-2):161-8
  8. Ref:11160924: Inagaki Y, Doolittle WF (2001) Class I release factors in ciliates with variant genetic codes. Nucleic acids research 29(4):921-7
  9. Ref:10648963: Karamyshev AL, Karamysheva ZN, Ito K, Matsufuji S, Nakamura Y (1999) Overexpression and purification of recombinant eRF1 proteins of rabbit and Tetrahymena thermophila. Biochemistry. Biokhimiia 64(12):1391-400
  10. Ref:10471834: Karamyshev AL, Ito K, Nakamura Y (1999) Polypeptide release factor eRF1 from Tetrahymena thermophila: cDNA cloning, purification and complex formation with yeast eRF3. FEBS letters 457(3):483-8
  11. Ref:1160924: Urbanek R, et al. (1975) [Hypophosphataemia as early sign of septicaemia in childhood (author's transl)] Monatsschr Kinderheilkd 123(8):593-7

Sequences 

>TTHERM_00292170(coding)
ATGGAAGAGAAAGATCAACGTTAAAGAAATATTGAACATTTTAAGATCAAGAAATTGATG
ACAAGACTTAGAAACACTAGAGGTTCTGGTACTTCTATGGTTTCTTTGATTATTCCTCCT
AAGAAGTAAATTAACGATTCCACAAAGTTAATCAGTGATGAATTCAGTAAGGCCACTAAT
ATTAAAGACAGAGTCAACCGTTAATCTGTACAAGATGCAATGGTTTCCGCCTTACAAAGA
TTAAAATTATATCAAAGAACTCCCAATAATGGTTTGATTCTTTATTGCGGTAAGGTTCTT
AACGAAGAAGGAAAAGAAATTAAGCTTTTGATCGATTTCGAACCCTACAAGCCCATCAAC
ACCTCTCTTTATTTCTGTGACAGTAAGTTCCACGTTGATGAATTGGGTTCACTTCTTGAA
ACCGACCCTCCTTTTGGTTTCATCGTTATGGATGGTCAAGGTGCTCTCTATGCCAATCTC
CAAGGAAATACAAAGACAGTTTTAAATAAATTCTCAGTCGAATTGCCCAAGAAGCACGGT
AGAGGTGGTCAATCATCAGTTCGTTTTGCACGTCTTAGAGTTGAAAAGAGACACAACTAT
CTTAGAAAGGTTTGTGAAGTCGCCACTTAAACTTTCATTTCCCAAGATAAAATTAATGTT
TAAGGTTTAGTCTTAGCTGGTTCTGGTGATTTCAAGAATGAATTGAGTACTACATAGATG
TTCGACCCTCGTCTTGCTTGCAAGATTATCAAGATTGTTGATGTTTCTTATGGTGGTGAA
AATGGTCTTAACCAAGCTATTGAACTCGCTTAAGAATCTTTAACTAACGTCAAGTTCGTT
TAAGAAAAGAACGTTATCTCCAAATTCTTCGATTGCATCGCTATTGACTCCGGTACCGTT
GTCTATGGTGTTCAAGATACTATGTAACTCTTATTAGATGGTGTTATTGAAAACATTCTT
TGTTTCGAAGAACTCACTACCTTGAGAGTCACTCGTAAAAATAAAGTCACAGAATAAATC
ACTCATATATTCATTCCTCCTAATGAATTAAATAATCCTAAGCATTTCAAGGATGGTGAA
CATGAACTTGAAAAGATTGAAGTTGAAAACTTAACTGAATGGTTAGCTGAACACTACAGT
GAATTCGGTGCTGAACTTTACTTTATTACTGATAAATCTGCTGAAGGTTGCCAATTTGTC
AAAGGTTTCTCTGGTATTGGTGGTTTCTTAAGATATAAGGTCGATTTAGAACATATTGTT
AACCCTAATGACGAATACAACTACGAAGAAGAAGAAGGCTTCATATGA


>TTHERM_00292170(gene)
ATGGAAGAGAAAGATCAACGTTAAAGAAATATTGAACATTTTAAGATCAAGAAATTGATG
ACAAGACTTAGAAACACTAGAGGGTAATCAATCTATTTAATATAAAAAATTATTTTCTCT
CTTTTCACTAACTTTCCATTTGCACCCACTGATTCAATATGTTATTTTTGTTTTGCTTGA
ATAATAATAAAACTATATATAGCCTAGTTATCATTCATTTAATTTTAATATCTTATGGAA
TTGCAATTCTATATTTTGCTTAAGGATAAATACCTTAAAAATATGTGACATCAGTGGAGA
TTTTGCATAATTTGGAAGATTTAGGGATTGAAATTTTAGAAAATTTTAAGTAAATAACCT
TTATAACTTTTAAAAACATAGTTCTGGTACTTCTATGGTTTCTTTGATTATTCCTCCTAA
GAAGTAAATTAACGATTCCACAAAGTTAATCAGTGATGAATTCAGTAAGGCCACTAATAT
TAAAGACAGAGTCAACCGTTAATCTGTACAAGATGCAATGGTTTCCGCCTTACAAAGTAA
AATTATTATATTATTGGTTAAATATGTATATATGCTTGGCATTTCTTCAAACAACATCCA
CAAAAACTATTGAATGATGAAGATATTTTAAGCAAAATTTAAAAAATGAAAATAAATTTG
CTGACTGGTCTTAAATGTTTAATTTATAAAACAAAGGATTAAAATTATATCAAAGAACTC
CCAATAATGGTTTGATTCTTTATTGCGGTAAGGTTCTTAACGAAGAAGGAAAAGAAATTA
AGCTTTTGATCGATTTCGAACCCTACAAGCCCATCAACACCTCTCTTTATTTCTGTGACA
GTAAGTTCCACGTTGATGAATTGGGTTCACTTCTTGAAACCGACCCTCCTTTTGGTTTCA
TCGTTATGGATGGTCAAGGTGCTCTCTATGCCAATCTCCAAGGAAATACAAAGACAGTAA
GCTATATAAAATAAATTAACTATCAAAATTTTTTATTAGATGCATGCAAAACAAATAAAC
AAGGAAAGATTTAATAAGGGTCAATTAAAAATACGCTTAAAATTATTAAAAAATATTAAC
GAAAGTCATATTTTTTAGGGATTAATTAACAAATAAATATTTTGGCTTTTAAGAAAATCT
TATTATATACTTTCTTTAATTAATAAGCATTTAATCCTATTTTCAATCAAAGCATATTCT
CAAAAAAAAAAAATACAAGCAAGCAAGCAAATATCTCTTTGAATATTTTTAAATAAAATC
AAATTGAGTAAATATATTTCTTCTATGTAGCAGAGTTTACTAAATTAGTAATATTACTAC
CAGTTTTTTAGTAAAAAATAAAACGAACTATCTTTCAAATGAATATATAAAAAAACATTA
TTCTTTTTAACATATAATCATTTCTCATTAATTAAAAAAGGTTTTAAATAAATTCTCAGT
CGAATTGCCCAAGAAGCACGGTAGAGGTGGTCAATCATCAGTTCGTTTTGCACGTCTTAG
AGTTGAAAAGAGACACAACTATCTTAGAAAGGTTTGTGAAGTCGCCACTTAAACTTTCAT
TTCCCAAGATAAAATTAATGTTTAAGGTTTAGTCTTAGCTGGTTCTGGTGATTTCAAGAA
TGAATTGAGTACTACATAGATGTTCGACCCTCGTCTTGCTTGCAAGATTATCAAGATTGT
TGATGTTTCTTATGGTGGTGAAAATGGTCTTAACCAAGCTATTGAACTCGCTTAAGAATC
TTTAACTAACGTCAAGTTCGTTTAAGAAAAGAACGTTATCTCCAAATTCTTCGATTGCAT
CGCTATTGACTCCGGTACCGTTGTCTATGGTGTTCAAGATACTATGTAACTCTTATTAGA
TGGTGTTATTGAAAACATTCTTTGTTTCGAAGAACTCACTACCTTGAGAGTCACTCGTAA
AAATAAAGTCACAGAATGTAATTATTTAGCATTTATTTCAATTTTCAGATTAGGTATTTG
AACTCAAAAAGCAAGCAATCAAGCAAAAAAGCAAACAAACAAACAAACAAAAAATAAATT
AAATAGCAAGAAAGAAAGAAAGAAAGAAATAAATAAATAAGGCTTTAATAGATGATACCA
ATAGAATCTAATAATAAAATCTTTTTCTACAAATAAATTTAATTAATTAAATGATTAAAA
ATATCTTTTATAAAATTAGAAATCACTCATATATTCATTCCTCCTAATGAATTAAATAAT
CCTAAGCATTTCAAGGATGGTGAACATGAACTTGAAAAGATTGAAGTTGAAAACTTAACT
GAATGGTTAGCTGAACACTACAGTGAATTCGGTGCTGAACTTTACTTTATTACTGATAAA
TCTGCTGAAGGTTGCCAATTTGTCAAAGGTTTCTCTGGTATTGGTGGTTTCTTAAGATAT
AAGGTCGATTTAGAACATATTGTTAACCCTAATGACGAATACAACTACGAAGAAGAAGAA
GGCTTCATATGA


>TTHERM_00292170(protein)
MEEKDQRQRNIEHFKIKKLMTRLRNTRGSGTSMVSLIIPPKKQINDSTKLISDEFSKATN
IKDRVNRQSVQDAMVSALQRLKLYQRTPNNGLILYCGKVLNEEGKEIKLLIDFEPYKPIN
TSLYFCDSKFHVDELGSLLETDPPFGFIVMDGQGALYANLQGNTKTVLNKFSVELPKKHG
RGGQSSVRFARLRVEKRHNYLRKVCEVATQTFISQDKINVQGLVLAGSGDFKNELSTTQM
FDPRLACKIIKIVDVSYGGENGLNQAIELAQESLTNVKFVQEKNVISKFFDCIAIDSGTV
VYGVQDTMQLLLDGVIENILCFEELTTLRVTRKNKVTEQITHIFIPPNELNNPKHFKDGE
HELEKIEVENLTEWLAEHYSEFGAELYFITDKSAEGCQFVKGFSGIGGFLRYKVDLEHIV
NPNDEYNYEEEEGFI