Identifiers and Description

Gene Model Identifier

TTHERM_00295700

Standard Name

FSF1 (Formaldehyde dehydrogenase/S-formylglutathione hydrolase fusion protein (test))

Aliases

PreTt15680 | 27.m00340 | 3692.m00143

Description

FSF1 S-(hydroxymethyl)glutathione dehydrogenase/class III alcohol dehydrogenase; Formaldehyde dehydrogenase/S-formylglutathione hydrolase fusion protein; fusion protein containing two enzymes known to catalyze sequential steps in the formaldehyde detoxification pathway in yeast; this fusion unique to ciliates; Alcohol dehydrogenase-like, N-terminal

Genome Browser (Macronucleus)

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Genome Browser (Micronucleus)

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External Links

Gene Ontology Annotations

Molecular Function

  • obsolete formaldehyde dehydrogenase (glutathione) activity (ISS) | GO:0004327
  • S-formylglutathione hydrolase activity (ISS) | GO:0018738

Biological Process

Domains

  • ( PF00107 ) Zinc-binding dehydrogenase
  • ( PF00756 ) Putative esterase
  • ( PF08240 ) Alcohol dehydrogenase GroES-like domain

Gene Expression Profile

Vegetative Cell Cycle (Zhang et al., 2023)

GeneMania

Tetrahymena Stock Center

No Data fetched for Tetrahymena Stock Center

Homologs

No Data fetched for Homologs

General Information

No. Gene Name(s) Paragraph Text
2221 FSF1 A macronuclear chromosome containing a fusion gene was cloned from the spirotrichous ciliate Oxytricha trifallax. The gene encodes a single polypeptide containing homologs of two proteins that catalyze sequential steps in the formaldehyde detoxification pathway in Saccharomyces cerevisiae. These two proteins are formaldehyde dehydrogenase (FALDH) and S-formylglutathione hydrolase (SFGH); the fusion gene is called FSF1 (FALDH/SFGH Fusion 1). A similar gene was identified in the Tetrahymena thermophila genome sequence, and a T. thermophila EST sequenced from both ends showed that the fusion gene is expressed in this species in vivo. FSF1 has not yet been identified in other ciliates, but a fusion of these two genes has been identified in another group of protists, the diatoms. An EST from Phaeodactylum tricornutum and a gene from the genome sequence of Thalassiosira pseudonana both encode a fusion of these two genes, but in the opposite orientation of the ciliate genes. In diatoms, the SFGH domain is found N-terminal to the FALDH domain, suggesting that these two fusion genes evolved independently in ciliates and diatoms. The diatom genes were named SFF1 to highlight these differences.

Associated Literature

  1. Ref:15858209: Stover NA, Cavalcanti AR, Li AJ, Richardson BC, Landweber LF (2005) Reciprocal fusions of two genes in the formaldehyde detoxification pathway in ciliates and diatoms. Molecular biology and evolution 22(7):1539-42

Sequences 

>TTHERM_00295700(coding)
ATGGACGCATCTTTACTCTCTGAATTCCTTAAAACTGCTGGCCAAACCATCACTTGTAAG
GCTGCTGTAGCTTGGGAAGCTAACAAACCTTTAGATTACACTGACATTTAAGTTGCTCCT
CCTAAGAAAGGAGAAGTTCGTATTAAAGTTTTTGCAAATGCTCTTTGCCATACTGATATT
TACACCTTAGAAGGACACGATCCTGAAGGTCTTTTCCCAAGCATTCTTGGTCATGAAGGT
ACAGGTATTGTTGAAAGTATTGGTGAAGGAGTTACTTCTGTTAAGCCTGGTGATATTGTT
ATTCCTTGCTACACACCTGAATGCAGGGAATTCTCTTGTATTTATTGTAACAATGAAAAC
ACTAACCTTTGTCCTAAGATTAGAGCCTTCTAAGGAAAGGGTTTGATGCCTGATGGTACA
AGCAGATTTTCTAAGGATGGTAAAACTATCTATCACTTTATGGGGTGTTCTAGTTTCTCT
GAATACACAGTTGTTGCTGAAATTTCTTGTGCTAAAGTTAGCGACAAGATTGATGTTAAC
CAAATGTGTCTTTTGGGTTGTGGTATCAGCACTGGTTGGGGTGCTGCAATTAATACTTGC
AAAGTAAGACCTGGATCAACAGTTGTCGTATTTGGTCTTGGTGCAGTTGGTTTAGCAGTA
GTTCAAGCTGCTAAAATTAAAGGAGCAAGAAAAATTGTTGGTATTGATCTTAACAGTGAA
AAATTCACTTTAGCTAAAGATTTCGGTTGCACTGAATGCTGGAATCCTAAAGAGTTGGAC
GGTAAGACCATTAAGGAACACCTTATTTCCTTAGAACCTTGGGGTTATGATTTCACATTT
GATTGCACTGGTATCACTGCTGTTATGAGAGATGCTTTAGAAATTGCTCACAGAGGATTT
GGTGAAAGTTGCGTTATTGGTGTTGCTGCTTCTGGTCATGAAATTTCTACTAGACCATTC
TAATTAGTTACTGGTAGATAATGGAAAGGAACTGCCTTTGGTGGATGGAAAAGCAGAACT
GAAGTTCCCTAATTAGTTTAACAAGTATCTACTGGGGAATTGCCAATTCAAAAATACATT
ACTCATGAAATTAAGGGCTTACAAAATGTCAACTAAGCTATTGATGCTTTACACTCAGGA
AAATGCTTGCGTGCAGTTGTTCATATCAACGAAAGACCTGATTTCAACAAAGAATAGGTA
TCAAAGTTAGTCCCTCGTGTTTATTCTAATGCAAGAAGTCATAATGGATGGGTAAAGAGA
GTAAAGCATTATTCTGAATCTCTTTAATGCGAAATGCAATTTTCAATTTACATCCCTGAT
AAGAAGTCTCGTAATGCTTAACTTCCCCCTGTTCTTTATTATCTTAGTGGACTTACTTGC
ACTGATGAAAACGCTTTGGTAAAATCTGGCTTCTAAAGATATGCCTCTGAACTCGGTATT
GCTGTTGTATTCCCTGACACCTCACCTCGTGTTGATATTGAAGGTGATAACGAAAGTTGG
GACTTTGGTAAATCAGCTGGATTCTACTTAAATGCTACTTAAGAAAAATGGAGAAAGAAC
TACAATATGTTCACCTATATCTCTGAAGAATTACCAAAATTGATTGGAGAATTCTTCCCT
GTTGATGTAAGCAGAGCTGGTATTACTGGTCACTCTATGGGTGGTATGGGAGCTCTTAAC
ATATTCTTAAAGAACCCAGGTAAATATTTGAGTGTCTCTGCCTTTGCTCCTATTTGCAAT
CCCACTTAGTGCCCTTGGGGAGTTAAGGCTTTCACTAACTATTTAGGCTCTGTAGAAGCT
GGTAAATAGTATGATCCAACTGAACTTATTGTTTAATATAATGGTCCCAAGGCTAAGATT
TTAATTGATCAAGGTACTCATGACTCATTCCTTTACAATTAACTTCATCCTTAAAATTTC
TTAAAGGCTGCATCTTCAGTTAATTACCCTGTTGAATTCAGATATCAAAATAACTATGAT
CACCTCTACTTCTTTGTAGAGACCTTCATGGGAGATCATTTCAAGCACCATGCTTAATAT
CTTCTTAGATGA



>TTHERM_00295700(gene)
ATCTTTCTAAATAATTAAATATTGAATCAATTTATAAATTGTCTAAAAAATTTTAAGTTT
TTCAAATGGACGCATCTTTACTCTCTGAATTCCTTAAAACTGCTGGCCAAACCATCACTT
GTAAGGCTGCTGTAGCTTGGGAAGCTAACAAACCTTTAGATTACACTGACATTTAAGTTG
CTCCTCCTAAGAAAGGAGAAGTTCGTATTAAAGTTTTTGCAAATGCTCTTTGCCATACTG
ATATTTACACCTTAGAAGGACACGATCCTGAAGGTCTTTTCCCAAGCATTCTTGGTCATG
AAGGTACAGGTATTGTTGAAAGTATTGGTGAAGGAGTTACTTCTGTTAAGCCTGGTGATA
TTGTTATTCCTTGCTACACACCTGAATGCAGGGAATTCTCTTGTATTTATTGTAACAATG
AAAACACTAACCTTTGTCCTAAGATTAGAGCCTTCTAAGGAAAGGGTTTGATGCCTGATG
GTACAAGCAGATTTTCTAAGGATGGTAAAACTATCTATCACTTTATGGGGTGTTCTAGTT
TCTCTGAATACACAGTTGTTGCTGAAATTTCTTGTGCTAAAGTTAGCGACAAGATTGATG
TTAACCAAATGTGTCTTTTGGGTTGTGGTATCAGCACTGGTTGGGGTGCTGCAATTAATA
CTTGCAAAGTAAGACCTGGATCAACAGTTGTCGTATTTGGTCTTGGTGCAGTTGGTTTAG
CAGTAGTTCAAGCTGCTAAAATTAAAGGAGCAAGAAAAATTGTTGGTATTGATCTTAACA
GTGAAAAATTCACTTTAGCTAAAGATTTCGGTTGCACTGAATGCTGGAATCCTAAAGAGT
TGGACGGTAAGACCATTAAGGAACACCTTATTTCCTTAGAACCTTGGGGTTATGATTTCA
CATTTGATTGCACTGGTATCACTGCTGTTATGAGAGATGCTTTAGAAATTGCTCACAGAG
GATTTGGTGAAAGTTGCGTTATTGGTGTTGCTGCTTCTGGTCATGAAATTTCTACTAGAC
CATTCTAATTAGTTACTGGTAGATAATGGAAAGGAACTGCCTTTGGTGGATGGAAAAGCA
GAACTGAAGTTCCCTAATTAGTTTAACAAGTATCTACTGGGGAATTGCCAATTCAAAAAT
ACATTACTCATGAAATTAAGGGCTTACAAAATGTCAACTAAGCTATTGATGCTTTACACT
CAGGAAAATGCTTGCGTGCAGTTGTTCATATCAACGAAAGACCTGATTTCAACAAAGAAT
AGGTATCAAAGTTAGTCCCTCGTGTTTATTCTAATGCAAGAAGTCATAATGGATGGGTAA
AGAGAGTAAAGCATTATTCTGAATCTCTTTAATGCGAAATGCAATTTTCAATTTACATCC
CTGATAAGAAGTCTCGTAATGCTTAACTTCCCCCTGTTCTTTATTATCTTAGTGGACTTA
CTTGCACTGATGAAAACGCTTTGGTAAAATCTGGCTTCTAAAGATATGCCTCTGAACTCG
GTATTGCTGTTGTATTCCCTGACACCTCACCTCGTGTTGATATTGAAGGTGATAACGAAA
GTTGGGACTTTGGTAAATCAGCTGGATTCTACTTAAATGCTACTTAAGAAAAATGGAGAA
AGAACTACAATATGTTCACCTATATCTCTGAAGAATTACCAAAATTGATTGGAGAATTCT
TCCCTGTTGATGTAAGCAGAGCTGGTATTACTGGTCACTCTATGGGTGGTATGGGAGCTC
TTAACATATTCTTAAAGAACCCAGGTAAATATTTGAGTGTCTCTGCCTTTGCTCCTATTT
GCAATCCCACTTAGTGCCCTTGGGGAGTTAAGGCTTTCACTAACTATTTAGGCTCTGTAG
AAGCTGGTAAATAGTATGATCCAACTGAACTTATTGTTTAATATAATGGTCCCAAGGCTA
AGATTTTAATTGATCAAGGTACTCATGACTCATTCCTTTACAATTAACTTCATCCTTAAA
ATTTCTTAAAGGCTGCATCTTCAGTTAATTACCCTGTTGAATTCAGATATCAAAATAACT
ATGATCACCTCTACTTCTTTGTAGAGACCTTCATGGGAGATCATTTCAAGCACCATGCTT
AATATCTTCTTAGATGATTTAAAATATAAATAAATTAATATCTATAAATTTTCTATTACA
TGTTTTTTACTGTGTTGAATAGTATAAAAATGAATGAACTAA



>TTHERM_00295700(protein)
MDASLLSEFLKTAGQTITCKAAVAWEANKPLDYTDIQVAPPKKGEVRIKVFANALCHTDI
YTLEGHDPEGLFPSILGHEGTGIVESIGEGVTSVKPGDIVIPCYTPECREFSCIYCNNEN
TNLCPKIRAFQGKGLMPDGTSRFSKDGKTIYHFMGCSSFSEYTVVAEISCAKVSDKIDVN
QMCLLGCGISTGWGAAINTCKVRPGSTVVVFGLGAVGLAVVQAAKIKGARKIVGIDLNSE
KFTLAKDFGCTECWNPKELDGKTIKEHLISLEPWGYDFTFDCTGITAVMRDALEIAHRGF
GESCVIGVAASGHEISTRPFQLVTGRQWKGTAFGGWKSRTEVPQLVQQVSTGELPIQKYI
THEIKGLQNVNQAIDALHSGKCLRAVVHINERPDFNKEQVSKLVPRVYSNARSHNGWVKR
VKHYSESLQCEMQFSIYIPDKKSRNAQLPPVLYYLSGLTCTDENALVKSGFQRYASELGI
AVVFPDTSPRVDIEGDNESWDFGKSAGFYLNATQEKWRKNYNMFTYISEELPKLIGEFFP
VDVSRAGITGHSMGGMGALNIFLKNPGKYLSVSAFAPICNPTQCPWGVKAFTNYLGSVEA
GKQYDPTELIVQYNGPKAKILIDQGTHDSFLYNQLHPQNFLKAASSVNYPVEFRYQNNYD
HLYFFVETFMGDHFKHHAQYLLR