Identifiers and Description

Gene Model Identifier

TTHERM_00299879

Standard Name

WAG1 (W And G motifs 1)

Aliases

3691.m01119

Description

WAG1 GW repeat protein; Twi1p-associated protein; interacts with Argonaute complex in RNAi; plays a role in DNA elimination; WAG1 Zinc finger CCCH domain-containing protein 9; hypothetical protein

Genome Browser (Macronucleus)

Full Screen View

No Genome Browser Data Present

Genome Browser (Micronucleus)

Full Screen View

No Genome Browser Data Present

External Links

• Uniprot
• Alphafold

Gene Ontology Annotations

Cellular Component

• ARC complex (IDA) | GO:0033167
• macronucleus (IDA) | GO:0031039

Biological Process

• programmed DNA elimination (IMP) | GO:0031049

Domains

No Data fetched for Domains

Gene Expression Profile

Vegetative Cell Cycle (Zhang et al., 2023)

GeneMania

No results were calculated for this gene in GeneMania.

Tetrahymena Stock Center

• ( SD02109 ) Macronucleus: Neo3 replaces coding of WAG1
• ( SD02110 ) Macronucleus: Neo3 replaces coding of WAG1
• ( SD02120 ) Micronucleus: heterozygous KO of WAG1 and cnjB Macronucleus: Complete KO of WAG1 and cnjB with neo3
• ( SD02121 ) Micronucleus: heterozygous KO of WAG1 and CnjB Macronucleus: Complete KO of WAG1 and CnjB with neo3
• ( SD02704 ) Micronucleus: Neo3 knockout of WAG1, heterozygous
• ( SD02734 ) Micronucleus: Neo3 knockout of WAG1
• ( SD02735 ) Micronucleus: heterozygous KO of WAG1 and TWI11, TWI1 also HA tagged Macronucleus: KO of WAG1 with neo3, HA tagged TWI11
• ( SD02736 ) Micronucleus: heterozygous KO of WAG1 and TWI11, TWI1 also HA tagged Macronucleus: KO of WAG1 with neo3, HA tagged TWI11

Homologs

No Data fetched for Homologs

General Information

No Data fetched for General Information

Associated Literature

1. Ref:29021213: Noto T, Mochizuki K (2017) Whats, hows and whys of programmed DNA elimination in Tetrahymena. Open biology 7(10):
2. Ref:26688337: Kataoka K, Mochizuki K (2015) Phosphorylation of an HP1-like Protein Regulates Heterochromatin Body Assembly for DNA Elimination. Developmental cell 35(6):775-88
3. Ref:20211138: Noto T, Kurth HM, Kataoka K, Aronica L, DeSouza LV, Siu KW, Pearlman RE, Gorovsky MA, Mochizuki K (2010) The Tetrahymena argonaute-binding protein Giw1p directs a mature argonaute-siRNA complex to the nucleus. Cell 140(5):692-703
4. Ref:19596782: Bednenko J, Noto T, DeSouza LV, Siu KW, Pearlman RE, Mochizuki K, Gorovsky MA (2009) Two GW repeat proteins interact with Tetrahymena thermophila argonaute and promote genome rearrangement. Molecular and cellular biology 29(18):5020-30

Sequences

>TTHERM_00299879(coding)
ATGGAAGTTTCTCTAGTTAATTAAGAAATGGAACAAAAAGATGATATTTCAATTGAAAGT
GGTTAGCTAAGTTGTGAGTAATTGGATGCAGCTAAGGACATCAATCACATGATTTAGAAT
AAGTAACCTGCTTTAGACGATAAAGAAGCAGAAGATGAAGAACCTAAAAGCTTTGTTTTT
GAAGAAATAAATACAGATAGAGTGCAGCAAATTCTACAAGAAAAATAAAATGAAAAGCCT
AAAAAGCAATCATCAGGAGAGAAAGATTCTATGCATTAAAATGGAAATGAAGATCAACCT
CTTAGATAATTATAAAGTGATATGAGAACAGATGACTCTAAACCTTTGAGAGAAAGTGCT
AATACATAGGTAGATTTTGCTAAAATGAGTAATTGTTCTACTACATGGGAATCTAATACT
AAAGATGAAACTTCAATTACATAAAATTAATCGATTTCAAACACTAGCGGAAGCGGATGG
GGAGGATAATCTTAAAATGAGTTAGTTCCTAAGTCAGATTCTTCCTCTAATATATGGGGA
GGCAGTTAAAACTCTATGGGAGCTTAAACAGAAAGTAAATCATCTTCTTCAGGATGGGGT
TCTAGTAATAAAGATAAAGTTAATAATGAATCCGTTCAATCTTCTAGTACAGGAGGATGG
GGAGCACAAAGCAATGATAATACATCAAGCTAAAATAATGAAGGAAATGGAGGTGGATGG
GGATCATCTACCAATAACAATCCATCATCATCAACAGCAGTATGGGGAAGTTCTACTTCT
GGAAATGAAGATAATTCCAATAAAAATAACTCAGAAGGTGGATGGGGTAACTAATCTACT
GATAGCGGAGGATGGGGTTCTTCAAATGGCAATGGAAGTAATAATAATAATAATAATAAT
AACTCAGGCGGAGGTAGATTTAATAGAAATAATAACAATAACAATGGAAATAATGGAGGT
GGAAGAAGATTCGATAGAAATAACAATAATGGAGAATCTGGAAATGATAACAATGGAGGA
TAAAGGAGATTCAACAACAACAATAATAATAGGAACTTTAACAAAGAAGGAGGAGAGAAT
AAAAATGGAAATAACGGAGGAGGCTGGAATAAGAATAACAACAACAGAAATGGCGGAGAA
CGCAATAATAATGGTGGAGGGTTTTAAAACCAAAATAGAAATAATTCATTCAAAAAGACC
AATTCAAACGAAGGTTAAAGTAACAACTCTGGTGGTTGGAATAGCTAATCTAATGATAAC
TCTTAATCTACAGGAGGAAGTGGCTGGGGAGCTTCCTCAAACGATGCTAATTAAAATAAT
ACAACCACTTCTTCTAGTGGATGGGGATCTTCTACAAATGAAAATAAAAACAGTTCTGAA
GCTTCTTCAGGTTGGGGTTCTTCGAATACTAATACAACAGCAAATACAAACTAAACCAGT
GAATCTAGTGGAGGATGGGGATCTTAATAAACTGAAAATAAATAAGAATCTCAATCAAGT
AGTGGAGGTTGGGGAAACACTTCTGATTAAACTCAAGGAGGAGGAGATTCCTGGGGTTCA
ACATCTACAAATAATAACAATAATAGCAATACTAATAATAACAACAGATTTAGAAATAAT
GGGAACAACAACTAGAATAACAATAATAATAGATAGGGAGGAAATAGAGCATGGGGCTAA
AATAAAGATTAGTAAGGAGGAAAGCAAGGAAACGGAAGTTGGGGAAACTCAAAACAAAAT
AACGAATAATAAGCTAATAAGAGTGATTCTTAAGATGGGTGGGGTAATAATAGTTCTAAT
AATGAAAATTCTACAACATCAGGAGGATGGGGGAATTAAGAATCCTCAAAAATAGAAAAT
TCAACTTAGTCTGGAACTGGTGGATGGGGCTCATCTTTCTCTAATAATAACAACAGCTCT
AGTGAAAATAATGGAGGAGGCAACAATGGTAAAAAATGGGGAGCATCTGACAATTAGAAT
TAGGGTGAGAATAAAAGATAAGGCTCATGGGGAAATGGAAATAATAGAAACAACTCAAAT
TAAAATTAAGGAGGAGAGAGATAAAATTTTAGGGGAAATAATAATAGAAATAATAATAAT
AATAATTCTTCTTTTGTAAATAACAATAAATAAAAAAGTAACTCTGGGGAAGGTGGTTGG
AATAACTCTTCAGACAATGCACCTTCAGTTTAAAGTGGAGGAGGTGGATGGGGATCTACT
ACTGAAGATAACACTACAGTATAAGGAGGTTGTGGGGGTGGATGGGGTTCATCTTAAGTA
AAAGAAGCAGCAAGTACTTAAGCAACAACAACATCAAGCTGGGGAGGTAATAATTAAGAT
TCTTTAAATAATGATAGCAAATTACTCACTAAAAGACCCTCGAAAGACCCTAGACTGTAA
GAAAAAAAATAAACTCAAGTACTTAGTATTAATGATATGTCAGAAACAGAAGTTTAGGAG
AGAATATTTGGTACTAAAAGAGTTAAAAAAAATGAAGAAGGTGAAGATTCCATTCTTTAA
GCTAAGTCTAAAATTCCATAATTCATAGATTTAGAAAAAGAGGAAGAAGGATGCTTTGCT
AGATTTTCTCCTTTGATTGTTGATAGGGATACTGACAAGGAGTATAATCCTACAGGTATA
CCTCTCGATGAGTAGATTTACGAAGGATCAATAAATTAACTAATTTCAAATAAAGACACT
ATAGATCAAGAGGATGGAGATGATGATGATCTAGAAATGGCTATAATTATTAATCCATAA
AAATTGTTGGAAAGTCAACTTAAAAAGTATTAAACATACTCCGGATTTTTCTAAGTATAT
AAGAAATATCTTGTCCCTGAAAAGTAGTCAATTCTTTGCGAACCCTGTGAAAACACAGCT
TACAATCTATTTCATGATAGACTAGTATACGTAATACCTGATGAGCCCAAGCGCTATAAC
ATTCTATGCTAGTTTAATACATTCATGCCATATGAGTAATATAGACATTTACCATTGATA
TCAACACGTAACAGAACTTCTTAATAGGAGCTTGGATTTTTAGACTATGAAACTGCTATT
GAATCTATCAAAAATATTTAAATACAAGAAACTAAAGAGAGAAGAACTATTTTCCCTTGC
TGGGTTGAGTCTTCATTACCAAACTAGCATGTTGAGCTCAACAAGCTCGCTAAATATTTG
CTTGATAAAAATATGGTAGCAGGTTATAACTACACCAACCATGCTACAATCAATCTGATG
CATGTGAAATAAATTTTTCCTGATTTAGAATATGTCGAATTACCTGAATAGTCTGAAAAC
TAGAAAACATCTCTTATTTGCCTTAACACTCTTGAAGCAAGTATTTACGACTCTTTTTGC
CAAAAAATACCTGCCTATGAATGA

>TTHERM_00299879(gene)
TTAGCTAAAAAAAAGTAATTTCATTTGCTTTTGGATTCTCACTAAAAATGTGTCTGTTTA
TATTCAAATTTTAATTTAATAGCTAGAAAGATTTTGCGGATTATTAAAATTAATAAATTA
ATTAGAATAAATTAAAAAAAAACATAAAAGATAATAAACATAATCTCAAACAAGAAAGTA
GAGCTTTAAGATAGATAAAGAGGCTATACCTGACGTGCAACTTTTAAGAAGAAGAATGGA
AGTTTCTCTAGTTAATTAAGAAATGGAACAAAAAGATGATATTTCAATTGAAAGTGGTTA
GCTAAGTTGTGAGTAATTGGATGCAGCTAAGGACATCAATCACATGATTTAGAATAAGTA
ACCTGCTTTAGACGATAAAGAAGCAGAAGATGAAGAACCTAAAAGCTTTGTTTTTGAAGA
AATAAATACAGATAGAGTGCAGCAAATTCTACAAGAAAAATAAAATGAAAAGCCTAAAAA
GCAATCATCAGGAGAGAAAGATTCTATGCATTAAAATGGAAATGAAGATCAACCTCTTAG
ATAATTATAAAGTGATATGAGAACAGATGACTCTAAACCTTTGAGAGAAAGTGCTAATAC
ATAGGTAGATTTTGCTAAAATGAGTAATTGTTCTACTACATGGGAATCTAATACTAAAGA
TGAAACTTCAATTACATAAAATTAATCGATTTCAAACACTAGCGGAAGCGGATGGGGAGG
ATAATCTTAAAATGAGTTAGTTCCTAAGTCAGATTCTTCCTCTAATATATGGGGAGGCAG
TTAAAACTCTATGGGAGCTTAAACAGAAAGTAAATCATCTTCTTCAGGATGGGGTTCTAG
TAATAAAGATAAAGTTAATAATGAATCCGTTCAATCTTCTAGTACAGGAGGATGGGGAGC
ACAAAGCAATGATAATACATCAAGCTAAAATAATGAAGGAAATGGAGGTGGATGGGGATC
ATCTACCAATAACAATCCATCATCATCAACAGCAGTATGGGGAAGTTCTACTTCTGGAAA
TGAAGATAATTCCAATAAAAATAACTCAGAAGGTGGATGGGGTAACTAATCTACTGATAG
CGGAGGATGGGGTTCTTCAAATGGCAATGGAAGTAATAATAATAATAATAATAATAACTC
AGGCGGAGGTAGATTTAATAGAAATAATAACAATAACAATGGAAATAATGGAGGTGGAAG
AAGATTCGATAGAAATAACAATAATGGAGAATCTGGAAATGATAACAATGGAGGATAAAG
GAGATTCAACAACAACAATAATAATAGGAACTTTAACAAAGAAGGAGGAGAGAATAAAAA
TGGAAATAACGGAGGAGGCTGGAATAAGAATAACAACAACAGAAATGGCGGAGAACGCAA
TAATAATGGTGGAGGGTTTTAAAACCAAAATAGAAATAATTCATTCAAAAAGACCAATTC
AAACGAAGGTTAAAGTAACAACTCTGGTGGTTGGAATAGCTAATCTAATGATAACTCTTA
ATCTACAGGAGGAAGTGGCTGGGGAGCTTCCTCAAACGATGCTAATTAAAATAATACAAC
CACTTCTTCTAGTGGATGGGGATCTTCTACAAATGAAAATAAAAACAGTTCTGAAGCTTC
TTCAGGTTGGGGTTCTTCGAATACTAATACAACAGCAAATACAAACTAAACCAGTGAATC
TAGTGGAGGATGGGGATCTTAATAAACTGAAAATAAATAAGAATCTCAATCAAGTAGTGG
AGGTTGGGGAAACACTTCTGATTAAACTCAAGGAGGAGGAGATTCCTGGGGTTCAACATC
TACAAATAATAACAATAATAGCAATACTAATAATAACAACAGATTTAGAAATAATGGGAA
CAACAACTAGAATAACAATAATAATAGATAGGGAGGAAATAGAGCATGGGGCTAAAATAA
AGATTAGTAAGGAGGAAAGCAAGGAAACGGAAGTTGGGGAAACTCAAAACAAAATAACGA
ATAATAAGCTAATAAGAGTGATTCTTAAGATGGGTGGGGTAATAATAGTTCTAATAATGA
AAATTCTACAACATCAGGAGGATGGGGGAATTAAGAATCCTCAAAAATAGAAAATTCAAC
TTAGTCTGGAACTGGTGGATGGGGCTCATCTTTCTCTAATAATAACAACAGCTCTAGTGA
AAATAATGGAGGAGGCAACAATGGTAAAAAATGGGGAGCATCTGACAATTAGAATTAGGG
TGAGAATAAAAGATAAGGCTCATGGGGAAATGGAAATAATAGAAACAACTCAAATTAAAA
TTAAGGAGGAGAGAGATAAAATTTTAGGGGAAATAATAATAGAAATAATAATAATAATAA
TTCTTCTTTTGTAAATAACAATAAATAAAAAAGTAACTCTGGGGAAGGTGGTTGGAATAA
CTCTTCAGACAATGCACCTTCAGTTTAAAGTGGAGGAGGTGGATGGGGATCTACTACTGA
AGATAACACTACAGTATAAGGAGGTTGTGGGGGTGGATGGGGTTCATCTTAAGTAAAAGA
AGCAGCAAGTACTTAAGCAACAACAACATCAAGCTGGGGAGGTAATAATTAAGATTCTTT
AAATAATGATAGCAAATTACTCACTAAAAGACCCTCGAAAGACCCTAGACTGTAAGAAAA
AAAATAAACTCAAGTACTTAGTATTAATGATATGTCAGAAACAGAAGTTTAGGAGAGAAT
ATTTGGTACTAAAAGAGTTAAAAAAAATGAAGAAGGTGAAGATTCCATTCTTTAAGCTAA
GTCTAAAATTCCATAATTCATAGATTTAGAAAAAGAGGAAGAAGGATGCTTTGCTAGATT
TTCTCCTTTGATTGTTGATAGGGATACTGACAAGGAGTATAATCCTACAGGTATACCTCT
CGATGAGTAGATTTACGAAGGATCAATAAATTAACTAATTTCAAATAAAGACACTATAGA
TCAAGAGGATGGAGATGATGATGATCTAGAAATGGCTATAATTATTAATCCATAAAAATT
GTTGGAAAGTCAACTTAAAAAGTATTAAACATACTCCGGATTTTTCTAAGTATATAAGAA
ATATCTTGTCCCTGAAAAGTAGTCAATTCTTTGCGAACCCTGTGAAAACACAGCTTACAA
TCTATTTCATGATAGACTAGTATACGTAATACCTGATGAGCCCAAGCGCTATAACATTCT
ATGCTAGTTTAATACATTCATGCCATATGAGTAATATAGACATTTACCATTGATATCAAC
ACGTAACAGAACTTCTTAATAGGAGCTTGGATTTTTAGACTATGAAACTGCTATTGAATC
TATCAAAAATATTTAAATACAAGAAACTAAAGAGAGAAGAACTATTTTCCCTTGCTGGGT
TGAGTCTTCATTACCAAACTAGCATGTTGAGCTCAACAAGCTCGCTAAATATTTGCTTGA
TAAAAATATGGTAGCAGGTTATAACTACACCAACCATGCTACAATCAATCTGATGCATGT
GAAATAAATTTTTCCTGATTTAGAATATGTCGAATTACCTGAATAGTCTGAAAACTAGAA
AACATCTCTTATTTGCCTTAACACTCTTGAAGCAAGTATTTACGACTCTTTTTGCCAAAA
AATACCTGCCTATGAATGAAAATAGTGTAAAAGAATAAAATTTCTCTATAAACTTCTATT
TTGAAATTGCTTACTCTACAAAATCTTATTATTAACATTTTTATGAAGTATACAAATAAA
TTAATGCTTATTTTTAATTTTTAAGTAGTT

>TTHERM_00299879(protein)
MEVSLVNQEMEQKDDISIESGQLSCEQLDAAKDINHMIQNKQPALDDKEAEDEEPKSFVF
EEINTDRVQQILQEKQNEKPKKQSSGEKDSMHQNGNEDQPLRQLQSDMRTDDSKPLRESA
NTQVDFAKMSNCSTTWESNTKDETSITQNQSISNTSGSGWGGQSQNELVPKSDSSSNIWG
GSQNSMGAQTESKSSSSGWGSSNKDKVNNESVQSSSTGGWGAQSNDNTSSQNNEGNGGGW
GSSTNNNPSSSTAVWGSSTSGNEDNSNKNNSEGGWGNQSTDSGGWGSSNGNGSNNNNNNN
NSGGGRFNRNNNNNNGNNGGGRRFDRNNNNGESGNDNNGGQRRFNNNNNNRNFNKEGGEN
KNGNNGGGWNKNNNNRNGGERNNNGGGFQNQNRNNSFKKTNSNEGQSNNSGGWNSQSNDN
SQSTGGSGWGASSNDANQNNTTTSSSGWGSSTNENKNSSEASSGWGSSNTNTTANTNQTS
ESSGGWGSQQTENKQESQSSSGGWGNTSDQTQGGGDSWGSTSTNNNNNSNTNNNNRFRNN
GNNNQNNNNNRQGGNRAWGQNKDQQGGKQGNGSWGNSKQNNEQQANKSDSQDGWGNNSSN
NENSTTSGGWGNQESSKIENSTQSGTGGWGSSFSNNNNSSSENNGGGNNGKKWGASDNQN
QGENKRQGSWGNGNNRNNSNQNQGGERQNFRGNNNRNNNNNNSSFVNNNKQKSNSGEGGW
NNSSDNAPSVQSGGGGWGSTTEDNTTVQGGCGGGWGSSQVKEAASTQATTTSSWGGNNQD
SLNNDSKLLTKRPSKDPRLQEKKQTQVLSINDMSETEVQERIFGTKRVKKNEEGEDSILQ
AKSKIPQFIDLEKEEEGCFARFSPLIVDRDTDKEYNPTGIPLDEQIYEGSINQLISNKDT
IDQEDGDDDDLEMAIIINPQKLLESQLKKYQTYSGFFQVYKKYLVPEKQSILCEPCENTA
YNLFHDRLVYVIPDEPKRYNILCQFNTFMPYEQYRHLPLISTRNRTSQQELGFLDYETAI
ESIKNIQIQETKERRTIFPCWVESSLPNQHVELNKLAKYLLDKNMVAGYNYTNHATINLM
HVKQIFPDLEYVELPEQSENQKTSLICLNTLEASIYDSFCQKIPAYE