Identifiers and Description

Gene Model Identifier

TTHERM_00433810

Standard Name

HEN1 (HEN1 (Hua ENhancer) homolog 1)

Aliases

PreTt26522 | 48.m00285 | 3831.m01622

Description

HEN1 small RNA 2-prime-O-methyltransferase; RNA methyltransferase; stabilizes scnRNA by 2'-O-methylation; ensures DNA elimination in Tetrahymena; colocalizes and interacts with TWI1; expressed during growth and early conjugation; homolog of Arabidopsis HEN1

Genome Browser (Macronucleus)

Full Screen View

No Genome Browser Data Present

Genome Browser (Micronucleus)

Full Screen View

No Genome Browser Data Present

External Links

• Uniprot
• Alphafold

Gene Ontology Annotations

Cellular Component

• macronucleus (IDA) | GO:0031039

Molecular Function

• RNA methyltransferase activity (IDA) | GO:0008173

Biological Process

• programmed DNA elimination (IDA) | GO:0031049
• RNA catabolic process (IMP) | GO:0006401
• snRNA 2'-O-methylation (IDA) | GO:1990437

Domains

No Data fetched for Domains

Gene Expression Profile

Vegetative Cell Cycle (Zhang et al., 2023)

GeneMania

No results were calculated for this gene in GeneMania.

Tetrahymena Stock Center

No Data fetched for Tetrahymena Stock Center

Homologs

Source	Identifier	Score
Tetrahymena borealis	EI9_04218.1	9.997093137033517e-262
	Description: hypothetical protein (420 aa)
Stentor Coeruleus	SteCoe_13961	8.533047625744066e-17
	Description: None
Oxytricha	Contig5211.0.g50	0.00000000000020009182674645818
	Description: hypothetical protein
WormBase	WBGene00015349	0.000000009996807949091551
	Description: locus:henn-1 Uncharacterized protein C02F5.6 status:Partially_confirmed UniProt:P34283 protein_id:CCD62631.1

General Information

No.	Gene Name(s)	Paragraph Text
109	HEN1, TWI1	Twi1p-associated scnRNAs are 2'-O-methylated at their 3' ends by the RNA methyltransferase Hen1p (Kurth & Mochizuki 2009).

Associated Literature

1. Ref:29021213: Noto T, Mochizuki K (2017) Whats, hows and whys of programmed DNA elimination in Tetrahymena. Open biology 7(10):
2. Ref:19240163: Kurth HM, Mochizuki K (2009) 2'-O-methylation stabilizes Piwi-associated small RNAs and ensures DNA elimination in Tetrahymena. RNA (New York, N.Y.) 15(4):675-85

Sequences

>TTHERM_00433810(coding)
ATGATTGAAGCTTATGAAACAGATGTGTTTATGGATCCTATTGGTATGAAAGTTTGGGAA
AAAAGGCACTAATATGTAGCAACTAAGTTATCGGCTCTGAATTGTAAAAGAGTTTTGGAT
ATGGGAACAAATACTTGCAAGCTCATATAAAGACTTTCAAGGAGTTTGTAATTCACTTAA
ATAGATGGTTTAGATATCGATGGCTAATTGCTTGAGACTCAAGGAATCCAAAATGCGAAG
CCAGATTTGATATAAAATTAATATGCCAGTATGAGAGACCATTAGCTAGTGGTAAATCTA
TACCAAGGCAGTGCTTTGAATAAGATTTAACATTTGAAGGATTAGCAATACGATGCTGTA
ATATTAGTTGAACTCATTGAGCACTTATAAGTAGAAGATGTTTTTCTTATTGAGTAAAAT
TTGTTTGGCTTTTTGAGGCCACAATTTGTGATTGTGACTACACCTAACTCTGATTTTAAT
GTTTATTTTAACTTTAAAGAATAAGGAGTATTGTTTAGGGACAAAGATCACAAATTTGAG
TGGTCCTAAAACTAGTTTTAGATTTGGGCCTAAAAAGTATGCTAAAATTATGGATACAAA
GTAATAGAGTTGACAGGAGTAGGCGAACATAAAACAGAAGGAACTAAGAACGGTTTTTGT
ACACAAATAGTTGTATTTGAGAAAGATACCCAACAAGAGAAGTATCTGAACTTTGCTTTC
TTCAACCTTCAAGAGGGAGAAATTCGTTAAGTATGTTAAATTCTGTACCCATTTGAGAGT
AAAGAATAACATTTTCAAAGAGAAGTAGTAGACAGTATCAGATATATTCTGCATATTACT
GATAAGTAAAATTAATTCGAAGATGGTTCATACTAAAATTATACTACTCTCTCTCGCATA
ATGCAAAATCATTCAATATCAAGTAATTGGTAAATTTAAGGTGATTACTTTAAACTTAAA
ACCTACATCCAGAATATTTCTGAGTTTTTGGTACATGAAAATTAGTTCAATTTTCAAGAA
TCTTTCGTCACTCTCAATTATTAAGCCTAAATGGAAGATGAAGAAAATGAAGATTAGCTT
GAAAGCGATTCTGAAAATGTAAAGATGCAATAATAACAATATTACTTTTCTAACGATAAT
TGTTTCTCCACAAAAGATACCACTTATAGCAGTTTTTCCACTGCAGATAACTTATTCTCC
TAAAAAATTTAACTTGGTTAGCAATAAATGGCTTTAGAGGAAATAGAATTAGAAGATACA
ATTGATTATTGA

>TTHERM_00433810(gene)
ATGATTGAAGCTTATGAAACAGATGTGTTTATGGATCCTATTGGTATGAAAGTTTGGGAA
AAAAGGCACTAATATGTAGCAACTAAGTTATCGGCTCTGAATTGTAAAAGAGTAAGATTA
AAAACACATTTGATATTAAGATTGCTATTAACCCTGATTTCATATTTTGTTAATAAATTA
AGGTTTTGGATATGGGAACAAATACTTGCAAGCTCATATAAAGACTTTCAAGGAGTTTGT
AATTCACTTAAATAGTAAAATATCATTTTTCAATAATATTTGAATAGATTAATTAGTTTT
ATTCTTTAGGATGGTTTAGATATCGATGGCTAATTGCTTGAGACTCAAGGAATCCAAGTA
TAAATAATTTTAAATACGATTATAAAATAAATAGTAAACATAAAACTTAGAATGCGAAGC
CAGATTTGATATAAAATTAATATGCCAGTATGAGAGACCATTAGCTAGTGGTAAATCTAT
ACCAAGGCAGTGCTTTGAATAAGATTTAACATTTGAAGGATTAGCAATACGATGCTGTAA
TATTAGTTGAACTCATTGAGCACTTATAAGTAGAAGATGTTTTTCTTATTGAGTAAAATT
TGTTTGGCTTTTTGAGGCCACAATTTGTGATTGTGACTACACCTAACTCTGATTTTAATG
TTTATTTTAACTTTAAGTAAAATCTTACAATTCTAATAAAGGAAATTTAATGAATTATTT
TTTATTTTCTAGAGAATAAGGAGTATTGTTTAGGGACAAAGATCACAAATTTGAGTGGTC
CTAAAACTAGTTTTAGATTTGGGCCTAAAAAGTATGCTAAAATTATGGTAAAACAAGAAG
ATAACTAAATGTTAAGTGAACTTATTATTTATTTTTAATTTGTTTAGGATACAAAGTAAT
AGAGTTGACAGGAGTAGGCGAACATAAAACAGAAGGAACTAAGAACGGTTTTTGTACACA
AATAGTTGTATTTGAGAAAGATACCCAACAAGAGAAGTATCTGAACTTTGCTTTCTTGTA
AAAATTATTGATAAATAGTTTATTTAATTATTATTTTTTTAACTGAAAAAAGCAACCTTC
AAGAGGGAGAAATTCGTTAAGTATGTTAAATTCTGTACCCATTTGAGAGTAAAGAATAAC
ATTTTCAAAGAGAAGTAGTAGACAGTATCAGGTATTTAATTTATTAAGAAATAGATTTTA
AATACTTTTTTGAGACTAAATATTTTTTATTTCTAATTAGATATATTCTGCATATTACTG
ATAAGTAAAATTAATTCGAAGATGGTTCATACTAAAATTATACTACTCTCTCTCGCATAA
TGCAAAATCATTCAATATCAAGTAATTGGTAAATTTAAGGTGATTACTTTAAACTTAAAA
CCTACATCCAGAATATTTCTGAGTTTTTGGTACATGAAAATTAGTTCAATTTTCAAGAAT
CTTTCGTCACTCTCAATTATTAAGCCTAAATGGAAGATGAAGAAAATGAAGATTAGCTTG
AAAGCGATTCTGAAAATGTAAAGATGCAATAATAACAATATTACTTTTCTAACGATAATT
GTTTCTCCACAAAAGATACCACTTATAGCAGTTTTTCCACTGCAGATAACTTATTCTCCT
AAAAAATTTAACTTGGTTAGCAATAAATGGCTTTAGAGGAAATAGAATTAGAAGATACAA
TTGATTATTGA

>TTHERM_00433810(protein)
MIEAYETDVFMDPIGMKVWEKRHQYVATKLSALNCKRVLDMGTNTCKLIQRLSRSLQFTQ
IDGLDIDGQLLETQGIQNAKPDLIQNQYASMRDHQLVVNLYQGSALNKIQHLKDQQYDAV
ILVELIEHLQVEDVFLIEQNLFGFLRPQFVIVTTPNSDFNVYFNFKEQGVLFRDKDHKFE
WSQNQFQIWAQKVCQNYGYKVIELTGVGEHKTEGTKNGFCTQIVVFEKDTQQEKYLNFAF
FNLQEGEIRQVCQILYPFESKEQHFQREVVDSIRYILHITDKQNQFEDGSYQNYTTLSRI
MQNHSISSNWQIQGDYFKLKTYIQNISEFLVHENQFNFQESFVTLNYQAQMEDEENEDQL
ESDSENVKMQQQQYYFSNDNCFSTKDTTYSSFSTADNLFSQKIQLGQQQMALEEIELEDT
IDY