Identifiers and Description

Gene Model Identifier

TTHERM_00467500

Standard Name

MBD1 (Fusion of methylthioribulose-phosphate dehydratase and dihydroxy-keto-methylthiopentene dioxygenase)

Aliases

PreTt18652 | 55.m00207 | 3710.m00056

Description

MBD1 methylthioribulose-1-phosphate dehydratase; Fusion of two genes involved in the methionine salvage pathway; methylthioribulose-1-phosphate dehydratase -mtnB; and 1-2-dihydroxy-3-keto-5-methylthiopentene dioxygenase , mtnD; Acireductone dioxygenase ARD family

Genome Browser (Macronucleus)

Full Screen View


Genome Browser (Micronucleus)

Full Screen View

External Links

Gene Ontology Annotations

No Data fetched for Gene Ontology Annotations

Domains

  • ( PF00596 ) Class II Aldolase and Adducin N-terminal domain
  • ( PF03079 ) ARD/ARD' family

Gene Expression Profile

Vegetative Cell Cycle (Zhang et al., 2023)

GeneMania

Tetrahymena Stock Center

No Data fetched for Tetrahymena Stock Center

Homologs

No Data fetched for Homologs

General Information

No. Gene Name(s) Paragraph Text
2225 MBD1 MBD1 is a gene fusion of two genes involved in the methionine salvage pathway: methylthioribulose-1-phosphate dehydratase -mtnB; and 1,2-dihydroxy-3-keto-5-methylthiopentene dioxygenase - mtnD. These enzymes catalyze non-consecutive steps in the pathway. Interestingly the gene that codes for the intervening enzyme in the pathway, mtnC, is missing from the genome of Tetrahymena. Complementation tests in yeast were used to show that MBD1 from Tetrahymena is able to do in one step what yeast does in three, since it can rescue yeast knockouts of mtnB, mtnC, or mtnD (Salim, Negritto and Cavalcanti 2009).

Associated Literature

  1. Ref:19851454: Salim HM, Negritto MC, Cavalcanti AR (2009) 1+1 = 3: a fusion of 2 enzymes in the methionine salvage pathway of Tetrahymena thermophila creates a trifunctional enzyme that catalyzes 3 steps in the pathway. PLoS genetics 5(10):e1000701

Sequences 

>TTHERM_00467500(coding)
ATGAACGGTTTATCTTCAAATTCTGGTCCTAGAGAAGTCATTTGTGCTCTCTTGAGAAAC
TTTTATACTTAAGGATGGTGCGCTGGTTCAGGTGGTGGTATATCTATTAGAAAAAGCGAC
GATGAAATATATGTTGCACCTTCAGGTGTATAAAAAGAATTGGTTTAACCTGAAGATATC
TATGTTATTAATGTTAATGGAGATGTAGTTGAAAACCCTAAAAATCCAAAATTAAAACCT
TCTGAATGCACTCCTCTTTTTAATGCAGCTTATAAGCTTCGTCGTGCTGGTGCTGTTTTA
CATTCTCATGCTCTTCCTGCAATGCTAGTTACCAAGCTCTTTGGAACTGAATTTTAGACA
ATTGATCATGAAATGATTAAAGGTATTCCTAATCACCATAATACAGAATGGTGTGTCGTC
CCTATCATTGAAAACACTGAAAAGGAATGCGAATTAACAGAACGTTTAACTAATGCAATC
AACGCTTATCCTCGTTCTAATGCTGTCTTAGTTCGTAATCACGGTGTTTATATTTGGGGA
GAAAATTGGGAAAAAGCAAAAATACATGCTGAGTGTTACCATTATTTATTCGAAGCAGTT
GTTGAAATGAAGAAATTAGGTTTAGAAATTCCACGCACTGTTTCAAGCAGTAGCCAATTA
CGTGCTTGGTATATTGATGAAAATGCAATTGGCCAAGATGGTGATATTCGTGAGAGCTTA
CACTATCGCTCATATAAGTGGGTTAATCCAGAATATTTGGCAACAATTGGTGTTGAACAC
TGGAAATTAGACGGAGAAGAGAATAATACTGAACTTGAAAGAGTTTGCAAATAAAGAAAT
TACTCTTCTCGTGATTAAATTAAATGTGGAAATCATTGTGAAAATTACTAACAAATGTTA
TCAAATTTCAGAAAAGAACACATCCATTTAGATGAAGAGATTCGCTATATTATAGGTGGT
AGTGGTTATTTTGATGTCCGTGACCATGAAGATAAATGGATTCGTATCCAAAGTGTTAAA
GGTGATTTAATTGTACTTCCTGAAGGTATATATCACCGCTTTACAACTGATAAAAATGAT
GGAGTTCATGCTATGAGATTATTTAAGGATGAACCTAAATGGACACCTTATAATCGTCCT
TGTGATGAATTGGAAAGCCGTAACAAATATGTAGATTAATTCTTAAAAAAGTTTTAAAAA
TGA



>TTHERM_00467500(gene)
TAGGATATAAAAAAAATAAATTAAAAGCTATAATTAACTATGAACGGTTTATCTTCAAAT
TCTGGTCCTAGAGAAGTCATTTGTGCTCTCTTGAGAAACTTTTATACTTAAGGATGGTGC
GCTGGTTCAGGTGGTGGTATATCTATTAGAAAAAGCGACGATGAAATATATGTTGCACCT
TCAGGTGTATAAAAAGAATTGGTTTAACCTGAAGATATCTATGTTATTAATGTTAATGGA
GATGTAGTTGAAAACCCTAAAAATCCAAAATTAAAACCTTCTGAATGCACTCCTCTTTTT
AATGCAGCTTATAAGCTTCGTCGTGCTGGTGCTGTTTTACATTCTCATGCTCTTCCTGCA
ATGCTAGTTACCAAGCTCTTTGGAACTGAATTTTAGACAATTGATCATGAAATGATTAAA
GGTATTCCTAATCACCATAATACAGAATGGTGTGTCGTCCCTATCATTGAAAACACTGAA
AAGGAATGCGAATTAACAGAACGTTTAACTAATGCAATCAACGCTTATCCTCGTTCTAAT
GCTGTCTTAGTTCGTAATCACGGTGTTTATATTTGGGGAGAAAATTGGGAAAAAGCAAAA
ATACATGCTGAGTGTTACCATTATTTATTCGAAGCAGTTGTTGAAATGAAGAAATTAGGT
TTAGAAATTCCACGCACTGTTTCAAGCAGTAGCCAATTACGTGCTTGGTATATTGATGAA
AATGCAATTGGCCAAGATGGTGATATTCGTGAGAGCTTACACTATCGCTCATATAAGTGG
GTTAATCCAGAATATTTGGCAACAATTGGTGTTGAACACTGGAAATTAGACGGAGAAGAG
AATAATACTGAACTTGAAAGAGTTTGCAAATAAAGAAATTACTCTTCTCGTGATTAAATT
AAATGTGGAAATCATTGTGAAAATTACTAACAAATGTTATCAAATTTCAGAAAAGAACAC
ATCCATTTAGATGAAGAGATTCGCTATATTATAGGTGGTAGTGGTTATTTTGATGTCCGT
GACCATGAAGATAAATGGATTCGTATCCAAAGTGTTAAAGGTGATTTAATTGTACTTCCT
GAAGGTATATATCACCGCTTTACAACTGATAAAAATGATGGAGTTCATGCTATGAGATTA
TTTAAGGATGAACCTAAATGGACACCTTATAATCGTCCTTGTGATGAATTGGAAAGCCGT
AACAAATATGTAGATTAATTCTTAAAAAAGTTTTAAAAATGATATCTTGACTAGTTTATA
TATCAAATCTTATATGTTAATATGAATTCAATAGTTGAAAATTTCAATTTATACCAAA



>TTHERM_00467500(protein)
MNGLSSNSGPREVICALLRNFYTQGWCAGSGGGISIRKSDDEIYVAPSGVQKELVQPEDI
YVINVNGDVVENPKNPKLKPSECTPLFNAAYKLRRAGAVLHSHALPAMLVTKLFGTEFQT
IDHEMIKGIPNHHNTEWCVVPIIENTEKECELTERLTNAINAYPRSNAVLVRNHGVYIWG
ENWEKAKIHAECYHYLFEAVVEMKKLGLEIPRTVSSSSQLRAWYIDENAIGQDGDIRESL
HYRSYKWVNPEYLATIGVEHWKLDGEENNTELERVCKQRNYSSRDQIKCGNHCENYQQML
SNFRKEHIHLDEEIRYIIGGSGYFDVRDHEDKWIRIQSVKGDLIVLPEGIYHRFTTDKND
GVHAMRLFKDEPKWTPYNRPCDELESRNKYVDQFLKKFQK