Identifiers and Description

Gene Model Identifier

TTHERM_00492850

Standard Name

ILS1 (IsoLeucine-tRNA Synthetase 1 )

Aliases

cupC | PreTt04221 | 61.m00208 | 3711.m00056 | ILSA

Description

ilsA isoleucyl-tRNA synthetase protein; Isoleucyl-tRNA synthetase; contains two C-terminal leucine zipper motifs and sequence similarity with the S.cerevisiae ileRS protein; transcript induced during meitoic prophase and nutritional shift-up but decreased by starvation

Genome Browser (Macronucleus)

Full Screen View

No Genome Browser Data Present

Genome Browser (Micronucleus)

Full Screen View

No Genome Browser Data Present

External Links

• Uniprot
• Alphafold

Gene Ontology Annotations

Cellular Component

• cytoplasm (ISS) | GO:0005737
• cytoplasm (IEA) | GO:0005737

Molecular Function

• aminoacyl-tRNA ligase activity (IEA) | GO:0004812
• ATP binding (IEA) | GO:0005524
• nucleotide binding (IEA) | GO:0000166

Biological Process

• translation (IEP) | GO:0006412
• translation (IEA) | GO:0006412
• tRNA aminoacylation for protein translation (IEA) | GO:0006418

Domains

• ( PF00133 ) tRNA synthetases class I (I, L, M and V)
• ( PF08264 ) Anticodon-binding domain of tRNA ligase
• ( PF09334 ) tRNA synthetases class I (M)
• ( TIGR00392 ) isoleucine--tRNA ligase
• ( TIGR00395 ) leucyl-tRNA synthetase
• ( TIGR00396 ) leucyl-tRNA synthetase
• ( TIGR00398 ) methionyl-tRNA synthetase
• ( TIGR00422 ) valyl-tRNA synthetase

Gene Expression Profile

Vegetative Cell Cycle (Zhang et al., 2023)

GeneMania

No results were calculated for this gene in GeneMania.

Tetrahymena Stock Center

No Data fetched for Tetrahymena Stock Center

Homologs

Source	Identifier	Score
Stentor Coeruleus	SteCoe_9795	0
	Description: None
WormBase	WBGene00002152	9.997093137033517e-262
	Description: locus:iars-1 Isoleucine--tRNA ligase, cytoplasmic status:Partially_confirmed UniProt:Q21926 protein_id:CAA94369.1
Tetrahymena borealis	EI9_12981.1	9.997093137033517e-262
	Description: isoleucyl-tRNA synthetase (1101 aa)
DictyBase	DDB_G0278293	9.997093137033517e-262
	Description: ileS on chromosome: 3 position 632971 to 636359
Oxytricha	Contig21964.0.g21	9.997093137033517e-262
	Description: tRNA synthetases class I (I, L, M and V)
SGD	YBL076C	9.997093137033517e-262
	Description: ILS1 Cytoplasmic isoleucine-tRNA synthetase, target of the G1-specific inhibitor reveromycin A
WormBase	WBGene00013694	9.997093137033517e-262
	Description: status:Confirmed UniProt:Q9XWQ5 protein_id:CAA21594.1
Tetrahymena borealis	EI9_03424.1	9.997093137033517e-262
	Description: ATP synthase F1, alpha subunit protein (547 aa)

General Information

No Data fetched for General Information

Associated Literature

1. Ref:1371507: Csank C, Martindale DW (1992) Isoleucyl-tRNA synthetase from the ciliated protozoan Tetrahymena thermophila. DNA sequence, gene regulation, and leucine zipper motifs. The Journal of biological chemistry 267(7):4592-9
2. Ref:2663194: Martindale DW, Gu ZM, Csank C (1989) Isolation and complete sequence of the yeast isoleucyl-tRNA synthetase gene (ILS1). Current genetics 15(2):99-106
3. Ref:3005971: Martindale DW, Martindale HM, Bruns PJ (1986) Tetrahymena conjugation-induced genes: structure and organization in macro- and micronuclei. Nucleic acids research 14(3):1341-54
4. Ref:6646127: Martindale DW, Bruns PJ (1983) Cloning of abundant mRNA species present during conjugation of Tetrahymena thermophila: identification of mRNA species present exclusively during meiosis. Molecular and cellular biology 3(10):1857-65

Sequences

>TTHERM_00492850(coding)
ATGATTCTTTTCAAAAAATTATTAATATAAAAAAAAGTAAATTATCTAAGCAGATTATTA
ATGGCTTCAAAGACCACCTTCAAAGATCTTTAGGAAATTCCTGATTTCCCTAAGGAAGAA
GAGAACATCCTCAAGTTTTGGGATGAAATTAATGCTTTTAAGCAACAATTAGAAAAAACT
AAGGATTGCCCTCCTTTCACTTTCTACGATGGCCCACCATTCGCTACCGGTTTACCTCAC
TATGGTAACCTTCTTGCTGGTACAATCAAGGATGTCGTTTGCAGATACGCTTCTCAAAAT
GGCAAATATGTTGAAAGAAGATTCGGTTGGGATTGTCACGGTCTTCCAGTTGAATATGAA
ATCGATAAGAAGCTCGGTATCACCAATAGACAAGAAGTCTTAAAGATGGGTGTCGACAAG
TACAATGCTGAATGCCGTAGCATCGTCATGAGATACGCTTAAGAATGGAGATCAATCGTG
AACAGATTTGGTCGTTGGGTTGACTTTGACAATGATTACAAAACTCTTGATTTGAAGTTT
ATGGAATCCGTTTGGTGGGTTTTCAAGCAAATGTTCGATAAGGGATTAGTTTATAGAGGT
TGCAAGGTTATGCCCTACTCCAATGGTTGTGCAACTGTCTTATCTAACTTCGAAACTCAA
CAAAACTACAAGGAAGTTGATGATCCTTCTCTCTTCATCGCTTTTAAGACTGCCGAAGAT
CCTAAGACCAAGTTCATTGCTTGGACAACCACTCCTTGGACACTTCCTTCTAACTTGGCT
TTGGTTATCAACAAGGACTTCGATTACGTTAAGGTTTTAGATGCTAAGACCTAAGAACAC
TACATTCTTGCAGAATGCCGTCTCCCTGAATTATACAAAAAGGATAAGGATGGATACAAG
ATCTTAGAAAAATTCAAAGGTTCTGAACTCGTAGGCAGAGAATATGAACCTCTTTTCCCC
TATTTCCTCTCTAGAAAGCAAGATGGCTGCTTCAGAATTCTCGCTGGTGACTTCGTTACT
GCTGATGCTGGTACTGGTATCGTCCACTGCGCCCCTGGTTTTGGTGATGACGATTACAAA
GTCTCAGTTGCAAATAACATCATCAAGCCCGATGATCCACCCGTACCTGTTGATGAAAAC
GGTCATTTCACTAATGTCGTTTCTGATTTTGCTGGTGTTTACATCAAGGAAGCTGATAAG
TTAATTAGAAAGAACTTGAAGGAAAGAGGTTTGTTGCTCGTTGATTCTTCATTCAAGCAC
AACTATCCCTTCTGCTGGAGATCTGATACCCCCTTGATTTACAAGGCTGTTCACTGCTGG
TTCATTAAGGTTACTGCCCTTAAGGATGACCTTTTAGCTAACAACAAGAAGGCTTATTGG
GTTCCTAAATTTGCTTAAGAAGGACGTTTCAACAACTGGTTGTAGAATGTCTCTGATTGG
TGTTTCTCTAGATCTAGATTCTGGGGTAACCCTATCCCTATTTGGGTTTCTGAAGATTTT
GAAGAAGTTGTTTGCATTGGATCAGTTGAAGAACTCAAGAAATTAACTGGTGCAACTGAA
ATCACTGATTTACACAAAGATTTTATTGATCACCTTACCATCCCATCCTAAAAGGGTAAG
GGGGTCTTAAGACGTATTGATGAAGTCTTTGATTGCTGGTTCGAATCTGGTTCTATGCCT
TATGGTCAATAACACTATCCCTTCTCTATGAACGAAGAAGAGTTCTCTAAGAGATTCCCT
GCTGATTTCATCGGTGAAGGTATTGATCAAACTAGAGGTTGGTTCTATACTCTTAACGTT
ATTTCTACTGCCTTAAGAAATTCCAACCCCTACAAAAACCTTATTGTTAACGGTATCGTT
CTTGCTGCTGACGGAAAGAAGATGTCTAAGTCAAAGAAGAACTACGATGATCCCTTGCTC
ATTGCCTCCAAGTACTCCGTTGATGCTATCCGTTTATATATGATCAACTCTCCTTTAGTC
AGAGCTGAAGAAATGAGTTTCAAGTCCGAAGGTGTTTTTGCTGTTAAGAAAGATATCTTC
TTACCCTGGTACAATGCTTACAAATTCTTAATCTAAAGTATCACTAGATGGGAACTCGCT
ACTGGAAAAGACTACATGTTCAACGAACAACTCTCTGTTGATACTACTAAATTAACTAAT
CCTACTGATAGATGGATTATTATCTCCTGCCAAAACTTGATCAACTATGTCAGAATTGAA
ATGGAAAAGTATCACTTGTACAATGTCGTTCCTCGTTTAATCCACTTCTTAGAAAACTTA
ACCAACTGGTATATTAGATTAAACAGAAACAGACTAAAAGGTGATTACGGTCTTGAAGAA
TAAGAAACTTCCCTCAACGTCTTATTCAACGTTATCTTAAACTCCACTATTTTAATGTCA
CCTTTAGTTCCTTTCATCACTGAAAGTTTCTATCAAAACTTAAGAAAGGTCATTCCTAAG
GGTTCTTCTTACTTAGAAGAATCTATCCACTTCTTGAGAATCCCTACTCCTAAGCAAGAA
CTTCTCGATGAAAAGATCGAAAGAAACTTTGAAAGAATGTAAAACATCATCAACTTTGCT
CGTACTTTAAGAGAAAAGAGAAAGGTCTCTCTTAAGCAACCTATCATGTCTTTGACTGTC
ATCAATTAAGACTAAGAATTCCACGATTCCTTAAAGGATTACATTCAATATATTGAAGAT
GAAATCAACACTCCTTCTATCCTCCACGAAATTAACACTGCTAATTATGTTGACTTAAAG
GCCATTCCTAATCACAAACTCCTTGGACAAAAACTTGGTAAGGAATACAATAAGGATCTT
AAGGCTGCTGCCGGTAACTTGTCTGCCAAGGATATTGAAACCTTGAAGACTACTGGATCT
ATTGATTTAGTTGGCAAGAAATTACTTTTGGAAGACTTCACCATTACTCAAAATTACAAG
AAAGAATACTCTAGTGGTGACTTAGAACTTGGTGGTGAAGGTGAAGTTATTTTACTTTTG
AACCTCGCTCAAGATGAAAAGTTAAAGAGCAAAGGTCTTGTTAGAGAATTTACCAGCAAC
GTTTAAAAGACCAAGAAGAAAACCGGCTTAAAGGTTGACGATAACATCGTTATTTATTAT
GATGTTACCAAAGCACCCAAGTTAAATGCTGCTATTTAATCCGATTTAGAAGCTGTCCAA
AAGGTCTTAAAGAAACCCCTCGTTCCTTTATCTGAAAAGAATGCTGCTCTTAAGATTGTT
GAAAACTCTTTACTTACTTTCTAGGTTGATGAAGAATCCGTTACTATCGAAATCGCTTAT
GCTTGA

>TTHERM_00492850(gene)
ATGATTCTTTTCAAAAAATTATTAATATAAAAAAAAGTAAATTATCTAAGCAGATTATTA
ATGGCTTCAAAGACCACCTTCAAAGATCGTAATAATTACTTCTACTAATAAATATGCCTT
TTTCTATGAAAAACAAAATCTATAAATGTCCTAGAAATTAATATTTTCTTTTAATACTCA
TATAGTTTAGGAAATTCCTGATTTCCCTAAGGAAGAAGAGAACATCCTCAAGTTTTGGGA
TGAAATTAATGCTTTTAAGCAACAATTAGAAAAAACTAAGGATTGCCCTCCTTTCACTTT
CTACGATGGCCCACCATTCGCTACCGGTTTACCTCACTATGGTAACCTTCTTGCTGGTAC
AATCAAGGTATTTTATAAAAATTTTTATTTATTTCAGAAAATTCTATTTAGTTTTTTGCT
CTTGCCATGTTTATTTTTCTGAAATAAAATCCTGTGTACGAAAAAAAAAAAACAAATTGA
CTAAAATAATTATTTTATTGGAGTCAACACATTTTTTTTTTCATTTTTTAAACTTAAACT
GTTTCTGAATTACTTAAATAAATAATATTAATATATTTTTAAATGCTTATAGGATGTCGT
TTGCAGATACGCTTCTCAAAATGGCAAATATGTTGAAAGAAGATTCGGTTGGGATTGTCA
CGGTCTTCCAGTTGAATATGAAATCGATAAGAAGCTCGGTATCACCAATAGACAAGAAGT
CTTAAAGGTAATATTTAAGATAAAAATTAGCATTTTTAGTTAATTCGAACCCTAATTTGG
CATTAGATATATTTCGAATACCATATTTTGCAAAGATGAAAATATTCAATTAAATTAAGC
TTTTGAAAGTTTAATTAATAAATAGATACAGAATAATAAATAAAGTATAAATCTTAGAAA
AAATTGGCGCAACTTCAATTAAAAAAAAAATTTTCATTCTTTTTTTTAAACAAAATATAA
TTTTCTTACTCTAATAAATCTTAAATAGAAATACATTTAATCTAAAAAATTATTTATTTT
TATATTAGATGGGTGTCGACAAGTACAATGCTGAATGCCGTAGCATCGTCATGAGATACG
CTTAAGAATGGAGATCAATCGTGAACAGATTTGGTCGGTAATGAAACTTCCTATTTAAAT
ATCTTCTTTAATTAAAATAAATTATTAATTCTAATTCAATAAATAAGAAAGGAAAATAAC
TAACGAATTAGCTTTTTAAATAAGCAAAGAAAAATTAAAATTTTAAAAACATAATTAATT
ATAGAAAATATAAATTTAATTCAATATTTATTAATTAATTATATTATAGTTGGGTTGACT
TTGACAATGATTACAAAACTCTTGATTTGAAGTTTATGGAATCCGTTTGGTGGGTTTTCA
AGCAAATGTTCGATAAGGGATTAGTTTATAGAGGTTGCAAGGTTATGCCCTACTCCAATG
GTTGTGCAACTGTCTTATCTAACTTCGAAACTCAACAAAACTACAAGGAAGTTGATGATC
CTTCTCTCTTCATCGCTTTTAAGACTGCCGAAGATCCTAAGACCAAGTTCATTGCTTGGA
CAACCACTCCTTGGACACTTCCTTCTAACTTGGCTTTGGTTATCAACAAGGACTTCGATT
ACGTTAAGGTTTTAGATGCTAAGACCTAAGAACACTACATTCTTGCAGAATGCCGTCTCC
CTGAATTATACAAAAAGGATAAGGATGGATACAAGATCTTAGAAAAATTCAAAGGTTCTG
AACTCGTAGGCAGAGAATATGAACCTCTTTTCCCCTATTTCCTCTCTAGAAAGCAAGATG
GCTGCTTCAGAATTCTCGCTGGTGACTTCGTTACTGCTGATGCTGGTACTGGTATCGTCC
ACTGCGCCCCTGGTTTTGGTGATGACGATTACAAAGTCTCAGTTGCAAATAACATCATCA
AGCCCGATGATCCACCCGTACCTGTTGATGAAAACGGTCATTTCACTAATGTCGTTTCTG
ATTTTGCTGGTGTTTACATCAAGGAAGCTGATAAGTTAATTAGAAAGAACTTGAAGGAAA
GAGGTTTGTTGCTCGTTGATTCTTCATTCAAGCACAACTATCCCTTCTGCTGGAGATCTG
ATACCCCCTTGATTTACAAGGCTGTTCACTGCTGGTTCATTAAGGTTACTGCCCTTAAGG
ATGACCTTTTAGCTAACAACAAGAAGGCTTATTGGGTAATTATTTAGATTGACATTTAAA
AATATTATTTTACTTAAATTATTCCAAATATATTAATTAGGTTCCTAAATTTGCTTAAGA
AGGACGTTTCAACAACTGGTTGTAGAATGTCTCTGATTGGTGTTTCTCTAGATCTAGATT
CTGGGGTAACCCTATCCCTATTTGGGTTTCTGAAGATTTTGAAGAAGTTGTTTGCATTGG
ATCAGTTGAAGAACTCAAGAAATTAACTGGTGCAACTGAAATCACTGATTTACACAAAGA
TTTTATTGATCACCTTACCATCCCATCCTAAAAGGGTAAGGGGGTCTTAAGACGTATTGA
TGAAGTCTTTGATTGCTGGTTCGAATCTGGTTCTATGCCTTATGGTCAATAACACTATCC
CTTCTCTATGAACGAAGAAGAGTTCTCTAAGAGATTCCCTGCTGATTTCATCGGTGAAGG
TATTGATCAAACTAGAGGTTGGTTCTATACTCTTAACGTTATTTCTACTGCCTTAAGAAA
TTCCAACCCCTACAAAAACCTTATTGTTAACGGTATCGTTCTTGCTGCTGACGGAAAGAA
GATGTCTAAGTCAAAGAAGAACTACGATGATCCCTTGCTCATTGCCTCCAAGTACTCCGT
TGATGCTATCCGTTTATATATGGTATTTATTTTCTTCTTTTAATAAAACCAAATTAATCA
TTTTAATTCCTTATCCTAAAGATCAACTCTCCTTTAGTCAGAGCTGAAGAAATGAGTTTC
AAGTCCGAAGGTGTTTTTGCTGTTAAGAAAGATATCTTCTTACCCTGGTACAATGCTTAC
AAATTCTTAATCTAAAGTATCACTAGATGGGAACTCGCTACTGGAAAAGACTACATGTTC
AACGAACAACTCTCTGTTGATACTACTAAATTAACTAATCCTACTGATAGATGGATTATT
GTAAGAATATTAAAATAGCTATTTTTTAATTTTAAAACAACTTTTACAAATAGATCTCCT
GCCAAAACTTGATCAACTATGTCAGAATTGAAATGGAAAAGTATCACTTGTACAATGTCG
TTCCTCGTTTAATCCACTTCTTAGAAAACTTAACCAACTGGTATATTAGATTAAACAGAA
ACAGACTAAAAGGTGATTACGGTCTTGAAGAATAAGAAACTTCCCTCAACGTCTTATTCA
ACGTTATCTTAAACTCCACTATTTTAATGTCACCTTTAGTTCCTTTCATCACTGAAAGTT
TCTATCAAAACTTAAGAAAGGTCATTCCTAAGGGTTCTTCTTACTTAGAAGAATCTATCC
ACTTCTTGAGAATCCCTACTCCTAAGCAAGAACTTCTCGATGAAAAGATCGAAAGAAACT
TTGAAAGAATGTAAAACATCATCAACTTTGCTCGTACTTTAAGAGAAAAGAGAAAGGTCT
CTCTTAAGCAACCTATCATGGTAATATTCAACAATTTATTATTATTATTAATGGTAATTA
ATTTTTAAAATCAAAGTCTTTGACTGTCATCAATTAAGACTAAGAATTCCACGATTCCTT
AAAGGATTACATTCAATATATTGAAGATGAAATCAACACTCCTTCTATCCTCCACGAAAT
TAACACTGCTAATTATGTTGACTTAAAGGCCATTCCTAATCACAAACTCCTTGGACAAAA
ACTTGGTAAGGAATACAATAAGGATCTTAAGGCTGCTGCCGGTAACTTGTCTGCCAAGGA
TATTGAAACCTTGAAGACTACTGGATCTATTGATTTAGTTGGCAAGAAATTACTTTTGGA
AGACTTCACCATTACTCAAAATTACAAGAAAGAATACTCTAGTGGTGACTTAGAACTTGG
TGGTGAAGGTGAAGTTATTTTACTTTTGAACCTCGCTCAAGATGAAAAGTTAAAGAGCAA
AGGTCTTGTTAGAGAATTTACCAGCAACGTTTAAAAGACCAAGAAGAAAACCGGCTTAAA
GGTTGACGATAACATCGTTATTTATTATGATGTTACCAAAGCACCCAAGTTAAATGCTGC
TATTTAATCCGATTTAGAAGCTGTCCAAAAGGTCTTAAAGAAACCCCTCGTTCCTTTATC
TGAAAAGAATGCTGCTCTTAAGATTGTTGAAAACTCTTTACTTACTTTCTAGGTTGATGA
AGAATCCGTTACTATCGAAATCGCTTATGCTTGA

>TTHERM_00492850(protein)
MILFKKLLIQKKVNYLSRLLMASKTTFKDLQEIPDFPKEEENILKFWDEINAFKQQLEKT
KDCPPFTFYDGPPFATGLPHYGNLLAGTIKDVVCRYASQNGKYVERRFGWDCHGLPVEYE
IDKKLGITNRQEVLKMGVDKYNAECRSIVMRYAQEWRSIVNRFGRWVDFDNDYKTLDLKF
MESVWWVFKQMFDKGLVYRGCKVMPYSNGCATVLSNFETQQNYKEVDDPSLFIAFKTAED
PKTKFIAWTTTPWTLPSNLALVINKDFDYVKVLDAKTQEHYILAECRLPELYKKDKDGYK
ILEKFKGSELVGREYEPLFPYFLSRKQDGCFRILAGDFVTADAGTGIVHCAPGFGDDDYK
VSVANNIIKPDDPPVPVDENGHFTNVVSDFAGVYIKEADKLIRKNLKERGLLLVDSSFKH
NYPFCWRSDTPLIYKAVHCWFIKVTALKDDLLANNKKAYWVPKFAQEGRFNNWLQNVSDW
CFSRSRFWGNPIPIWVSEDFEEVVCIGSVEELKKLTGATEITDLHKDFIDHLTIPSQKGK
GVLRRIDEVFDCWFESGSMPYGQQHYPFSMNEEEFSKRFPADFIGEGIDQTRGWFYTLNV
ISTALRNSNPYKNLIVNGIVLAADGKKMSKSKKNYDDPLLIASKYSVDAIRLYMINSPLV
RAEEMSFKSEGVFAVKKDIFLPWYNAYKFLIQSITRWELATGKDYMFNEQLSVDTTKLTN
PTDRWIIISCQNLINYVRIEMEKYHLYNVVPRLIHFLENLTNWYIRLNRNRLKGDYGLEE
QETSLNVLFNVILNSTILMSPLVPFITESFYQNLRKVIPKGSSYLEESIHFLRIPTPKQE
LLDEKIERNFERMQNIINFARTLREKRKVSLKQPIMSLTVINQDQEFHDSLKDYIQYIED
EINTPSILHEINTANYVDLKAIPNHKLLGQKLGKEYNKDLKAAAGNLSAKDIETLKTTGS
IDLVGKKLLLEDFTITQNYKKEYSSGDLELGGEGEVILLLNLAQDEKLKSKGLVREFTSN
VQKTKKKTGLKVDDNIVIYYDVTKAPKLNAAIQSDLEAVQKVLKKPLVPLSEKNAALKIV
ENSLLTFQVDEESVTIEIAYA