Identifiers and Description

Gene Model Identifier

TTHERM_00529790

Standard Name

CIT1 (CITrate synthase 1)

Aliases

PreTt04648 | 67.m00163 | 3684.m00033

Description

CIT1 citrate synthase I; Citrate synthase; bifunctional 14-nm filament-forming protein; structural protein involved in oral morphogenesis and pronuclear behavior during conjugation; citrate synthase activity decreased by polymerization and dephosphorylation

Genome Browser (Macronucleus)

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Genome Browser (Micronucleus)

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External Links

Gene Ontology Annotations

Cellular Component

Molecular Function

Biological Process

  • obsolete cellular carbohydrate metabolic process (IEA) | GO:0044262

Domains

  • ( PF00285 ) Citrate synthase, C-terminal domain
  • ( TIGR00165 ) ribosomal protein S18
  • ( TIGR00208 ) flagellar protein FliS
  • ( TIGR01793 ) citrate (Si)-synthase, eukaryotic
  • ( TIGR01798 ) citrate (Si)-synthase
  • ( TIGR01800 ) 2-methylcitrate synthase/citrate synthase II

Gene Expression Profile

Vegetative Cell Cycle (Zhang et al., 2023)

GeneMania

Tetrahymena Stock Center

No Data fetched for Tetrahymena Stock Center

Homologs

Source Identifier Score
Tetrahymena borealis EI9_13671.1 9.997093137033517e-262
Description: citrate synthase (463 aa)
SGD YNR001C 2.0004037638327014e-151
Description: CIT1 Citrate synthase, catalyzes the condensation of acetyl coenzyme A and oxaloacetate to form citrate; the rate-limiting enzyme of the TCA cycle; nuclear encoded mitochondrial protein
DictyBase DDB_G0275311 6.998976675167566e-150
Description: DDB_G0275311 on chromosome: 2 position 5329126 to 5331271
WormBase WBGene00000833 2.0000633521090825e-149
Description: locus:cts-1 Probable citrate synthase, mitochondrial status:Confirmed UniProt:P34575 protein_id:CAA83004.1
Oxytricha Contig5416.0.g81 9.003994373438572e-143
Description: Citrate synthase
Stentor Coeruleus SteCoe_31134 1.0648786602415064e-63
Description: None

General Information

No Data fetched for General Information

Associated Literature

  1. Ref:15170257: Mukai A, Endoh H (2004) Presence of a bacterial-like citrate synthase gene in Tetrahymena thermophila: recent lateral gene transfers (LGT) or multiple gene losses subsequent to a single ancient LGT? Journal of molecular evolution 58(5):540-9
  2. Ref:12025402: Kojima H, Numata O (2002) Enzymatic form and cytoskeletal form of bifunctional Tetrahymena 49kDa protein is regulated by phosphorylation. Zoological science 19(1):37-42
  3. Ref:11260259: Takeda T, Yoshihama I, Numata O (2001) Identification of Tetrahymena hsp60 as a 14-nm filament protein/citrate synthase-binding protein and its possible involvement in the oral apparatus formation. Genes to cells : devoted to molecular & cellular mechanisms 6(2):139-49
  4. Ref:9443816: Kojima H, Watanabe Y, Numata O (1997) The dual functions of Tetrahymena citrate synthase are due to the polymorphism of its isoforms. Journal of biochemistry 122(5):998-1003
  5. Ref:9268687: Takeda T, Watanabe Y, Numata O (1997) Direct demonstration of the bifunctional property of Tetrahymena 14-nm filament protein/citrate synthase following expression of the gene in Escherichia coli. Biochemical and biophysical research communications 237(2):205-10
  6. Ref:8575889: Numata O (1996) Multifunctional proteins in Tetrahymena: 14-nm filament protein/citrate synthase and translation elongation factor-1 alpha. International review of cytology 164( ):1-35
  7. Ref:7737352: Numata O, Yasuda T, Watanabe Y (1995) Tetrahymena intramitochondrial filamentous inclusions contain 14-nm filament protein/citrate synthase. Experimental cell research 218(1):123-31
  8. Ref:7592552: Takeda T, Kurasawa Y, Watanabe Y, Numata O (1995) Polymerization of highly purified Tetrahymena 14-nm filament protein/citrate synthase into filaments and its possible role in regulation of enzymatic activity. Journal of biochemistry 117(4):869-74
  9. Ref:8537311: Kojima H, Chiba J, Watanabe Y, Numata O (1995) Citrate synthase purified from Tetrahymena mitochondria is identical with Tetrahymena 14-nm filament protein. Journal of biochemistry 118(1):189-95
  10. Ref:1385189: Kurasawa Y, Numata O, Katoh M, Hirano H, Chiba J, Watanabe Y (1992) Identification of Tetrahymena 14-nm filament-associated protein as elongation factor 1 alpha. Experimental cell research 203(1):251-8
  11. Ref:1993043: Numata O, Takemasa T, Takagi I, Hirono M, Hirano H, Chiba J, Watanabe Y (1991) Tetrahymena 14-nm filament-forming protein has citrate synthase activity. Biochemical and biophysical research communications 174(2):1028-34
  12. Ref:3883198: Numata O, Sugai T, Watanabe Y (1985) Control of germ cell nuclear behaviour at fertilization by Tetrahymena intermediate filament protein. Nature 314(6007):192-4
  13. Ref:6401983: Fabregat I, Satrústegui J, Machado A (1983) Citrate synthase of Tetrahymena pyriformis: evolutionary and regulatory aspects. Archives of biochemistry and biophysics 220(2):354-60
  14. Ref:6414830: Numata O, Hirono M, Watanabe Y (1983) Involvement of Tetrahymena intermediate filament protein, a 49K protein, in the oral morphogenesis. Experimental cell research 148(1):207-20
  15. Ref:6807972: Numata O, Watanabe Y (1982) In vitro assembly and disassembly of 14-nm filament from Tetrahymena pyriformis. The protein component of 14-nm filament is a 49,000-Dalton protein. Journal of biochemistry 91(5):1563-73
  16. Ref:14168: Levy MR, McConkey CL (1977) Enzyme inactivation by a cellular neutral protease: enzyme specificity, effects of ligands on inactivation, and implications for the regulation of enzyme degradation. Journal of cellular physiology 90(2):253-63

Sequences 

>TTHERM_00529790(coding)
ATGAGATCTATCAACTAACTTCTTAAGCAAGCTAGCTTATCCCAAAAGAGCCAATACAAC
TTTAGTCAAACCAACTTAAAGAAGGTTATTGCTGAAATTATTCCTCAAAAGCAAGCTGAA
TTAAAGGAAGTCAAGGAAAAGTATGGTGATAAGGTTGTTGGTTAATACACTGTCAAGTAA
GTCATCGGTGGTATGAGAGGTATGAAGGGTCTTATGAGTGATCTCTCTCGTTGCGACCCC
TATCAAGGTATTATTTTCAGAGGCTACACTATTCCTCAACTTAAGGAATTCTTGCCTAAG
GCTGATCCTAAGGCTGCTGACTAAGCCAACTAAGAACCTTTACCTGAAGGTATTTTCTGG
TTATTAATGACCGGTTAATTACCCACCCACGCTTAAGTCGATGCTCTTAAGCACGAATGG
TAAAACAGAGGTACCGTCAACTAAGACTGTGTCAACTTCATTTTGAACTTACCCAAGGAC
CTCCACTCCATGACCATGCTCTCTATGGCCCTCCTTTACCTCCAAAAGGATTCTAAGTTC
GCTAAGCTTTACGACGAAGGTAAGATCTCCAAGAAAGATTACTGGGAGCCCTTCTACGAA
GATTCCATGGACCTTATTGCTAAGATCCCCAGAGTTGCTGCTATTATCTACAGACATAAA
TACAGAGATTCCAAGTTAATTGACTCTGATTCTAAGTTAGATTGGGCTGGTAACTACGCT
CATATGATGGGTTTCGAATAACACGTCGTTAAGGAATGTATTAGAGGTTATTTGAGCATC
CACTGTGATCACGAAGGTGGTAATGTTTCTGCCCATACTACTCACTTGGTCGGTTCTGCT
CTTTCTGATCCTTATCTTTCTTACTCTGCTGGTGTTAACGGTCTTGCCGGTCCCCTCCAC
GGTCTTGCTAACTAAGAAGTCCTTAAGTGGTTACTCTAATTCATTGAAGAAAAGGGTACC
AAGGTTTCTGACAAGGACATTGAAGACTACGTTGATCACGTTATTTCTTCTGGAAGAGTC
GTTCCTGGTTACGGACACGCTGTCTTGAGAGATACCGATCCTAGATTCCACCATTAAGTC
GATTTCTCCAAGTTCCACTTGAAGGATGACTAAATGATCAAATTATTACATTAATGTGCT
GATGTTATCCCCAAGAAATTATTAACTTACAAGAAGATTGCTAATCCTTATCCCAATGTT
GATTGCCACTCTGGTGTTTTATTATACTCTTTGGGCTTAACTGAATATCAATATTACACT
GTTGTCTTCGCCGTTTCTAGAGCTCTTGGTTGTATGGCTAACCTCATCTGGTCTAGAGCT
TTCGGTTTACCCATTGAAAGACCTGGCTCTGCTGATCTTAAGTGGTTCCACGACAAGTAC
AGAGAATGA


>TTHERM_00529790(gene)
ATGAGATCTATCAACTAACTTCTTAAGCAAGCTAGCTTATCCCAAAAGAGCCAATACAAC
TTTAGTCAAACCAACTTAAAGAAGGTTATTGCTGAAATTATTCCTCAAAAGCAAGCTGAA
TTAAAGGAAGTCAAGGAAAAGTATGGTGATAAGGTTGTTGGTTAATACACTGTCAAGTAA
GTCATCGGTGGTATGAGAGGTATGAAGGGTCTTATGAGTGATCTCTCTCGTTGCGACCCC
TATCAAGGTATTATTTTCAGAGGCTACACTATTCCTCAACTTAAGGAATTCTTGCCTAAG
GCTGATCCTAAGGCTGCTGACTAAGCCAACTAAGAACCTTTACCTGAAGGTATTTTCTGG
TTATTAATGACCGGTTAATTACCCACCCACGCTTAAGTCGATGCTCTTAAGCACGAATGG
TAAAACAGAGGTACCGTCAACTAAGACTGTGTCAACTTCATTTTGAACTTACCCAAGGAC
CTCCACTCCATGACCATGCTCTCTATGGCCCTCCTTTACCTCCAAAAGGATTCTAAGTTC
GCTAAGCTTTACGACGAAGGTAAGATCTCCAAGAAAGATTACTGGGAGCCCTTCTACGAA
GATTCCATGGACCTTATTGCTAAGATCCCCAGAGTTGCTGCTATTATCTACAGACATAAA
TACAGAGTAAAATTAATAGAATTTTTGTTTCTTAGCTTTATATTTAAATCTACTTTTAGA
AATATCTAACAATATGCTTTTTATCTTCTAATGAATTCAATAAAAAACTAATTTTAAGCA
TGTTAGTTTTTGAAATCGTTTTTAAAATACAATAGAATTTTTTTTCTTCTAGTGCTACCC
TACACTGATTTACTGATAGCTATTTATATAAGCATCTTAATTTTAACTGTATTAACAAAA
TTTCATTTATATTATAGGATTCCAAGTTAATTGACTCTGATTCTAAGTTAGATTGGGCTG
GTAACTACGCTCATATGATGGGTTTCGAATAACACGTCGTTAAGGAATGTATTAGAGGTT
ATTTGAGCATCCACTGTGATCACGAAGGTGGTAATGTTTCTGCCCATACTACTCACTTGG
TCGGTTCTGCTCTTTCTGATCCTTATCTTTCTTACTCTGCTGGTGTTAACGGTCTTGCCG
GTCCCCTCCACGGTCTTGCTAACTAAGAAGTCCTTAAGTGGTTACTCTAATTCATTGAAG
AAAAGGGTACCAAGGTTTCTGACAAGGACATTGAAGACTACGTTGATCACGTTATTTCTT
CTGGAAGAGTCGTTCCTGGTAAATTTGAATTTAAATTATATTATTTATAAATAGCAATCA
ATCATCATTGTCATAAAAATCGCAAGTTAAGTTAAAAATATAAGCAAAATTTAATATTTT
TTATAGAAAGAGTTTAGCTATTAAAGAAATAGCAAAGATAAGATTTAGATAAATAAGCAC
TTCAATAGTATTAGCACAAAATAAAACTACTTATTAAGCACAGTTAATCATAAAATTAAA
AATAAAAATTATTTACAACAAATATTTAGTAGACTTACCTCACATATTAAGATAAATAAA
TTATCTCGAAAACATCAAGAATCTTAAATAAAGATTAATAAAAGCAATCAATCACTTGCC
AGAATTTTCTGATCACAATTTATATTAAAATAATAAAGAAATAAATAAATAATGGAAGGA
AATATAATTCATAAATAAATTAAGTATTTATGAAAAAATTAAAAAAAAAAATTATTCTTA
ACATCACATCAATTAAGACTTTCCAGTTTGATAAAAAACATAAACGGCATAATATTATTT
GATGATTGATTATTACGATAATAGCATAAGCTTATAAAATTTCATACTTTTTAAACACTT
TTATAATACAATTCCTAAATTGTTATTACTAAACAATAGGTTACGGACACGCTGTCTTGA
GAGATACCGATCCTAGATTCCACCATTAAGTCGATTTCTCCAAGTTCCACTTGAAGGATG
ACTAAATGATCAAATTATTACATTAATGTGCTGATGTTATCCCCAAGAAATTATTAACTT
ACAAGAAGATTGCTAATCCTTATCCCAATGTTGATTGCCACTCTGGTGTTTTATTATACT
CTTTGGGCTTAACTGAATATCAATATTACACTGTTGTCTTCGCCGTTTCTAGAGCTCTTG
GTTGTATGGCTAACCTCATCTGGTCTAGAGCTTTCGGTTTACCCATTGAAAGACCTGGCT
CTGCTGATCTTAAGTGGTTCCACGACAAGTACAGAGAATGA


>TTHERM_00529790(protein)
MRSINQLLKQASLSQKSQYNFSQTNLKKVIAEIIPQKQAELKEVKEKYGDKVVGQYTVKQ
VIGGMRGMKGLMSDLSRCDPYQGIIFRGYTIPQLKEFLPKADPKAADQANQEPLPEGIFW
LLMTGQLPTHAQVDALKHEWQNRGTVNQDCVNFILNLPKDLHSMTMLSMALLYLQKDSKF
AKLYDEGKISKKDYWEPFYEDSMDLIAKIPRVAAIIYRHKYRDSKLIDSDSKLDWAGNYA
HMMGFEQHVVKECIRGYLSIHCDHEGGNVSAHTTHLVGSALSDPYLSYSAGVNGLAGPLH
GLANQEVLKWLLQFIEEKGTKVSDKDIEDYVDHVISSGRVVPGYGHAVLRDTDPRFHHQV
DFSKFHLKDDQMIKLLHQCADVIPKKLLTYKKIANPYPNVDCHSGVLLYSLGLTEYQYYT
VVFAVSRALGCMANLIWSRAFGLPIERPGSADLKWFHDKYRE