Identifiers and Description

Gene Model Identifier

TTHERM_00647330

Standard Name

THD1 (Tetrahymena Histone Deacetylase 1)

Aliases

PreTt15839 | 93.m00135 | 3727.m00055

Description

THD1 histone deacetylase family protein; Histone deacetylase; homolog of yeast Rpd3p; localized to the macronucleus during vegetative growth; deacetylates all four core histones in vitro; knockdown cells exhibit extrusion bodies and reduced phosphorylation of histone H1; hypothetical protein

Genome Browser (Macronucleus)

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Genome Browser (Micronucleus)

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External Links

Gene Ontology Annotations

Cellular Component

Biological Process

Domains

No Data fetched for Domains

Gene Expression Profile

Vegetative Cell Cycle (Zhang et al., 2023)

GeneMania

Tetrahymena Stock Center

No Data fetched for Tetrahymena Stock Center

Homologs

No Data fetched for Homologs

General Information

No. Gene Name(s) Paragraph Text
2220 THD1 Tetrahymena cells with reduced Thd1p expression exhibited phenotypes indicative of loss of chromatin integrity, such as DNA fragmentation and extrusion of chromatin from the macronucleus, variable macronuclear size and shape, enlarged nucleoli, and reduced phosphorylation of histone H1 from bulk chromatin. Macronuclei in THD1 knockdown cells also contained more DNA, suggesting Thd1p may play a role in regulating macronuclear DNA content. The THD1 gene could not be completely replaced by a disruption construct, suggesting that THD1 is an essential gene.
2219 THD1 THD1, a homolog of the Saccharomyces cerevisiae Rpd3p, a class I histone deacetylase (HDAC), is localized to the macronucleus during vegetative growth, and distributed to developing new macronuclei early in their differentiation. Thd1p deacetylates all four core histones in vitro. Thd1p is a 52kDa polypeptide in an HDAC complex of approximately 160 kDa.

Associated Literature

  1. Ref:34086947: Nabeel-Shah S, Garg J, Saettone A, Ashraf K, Lee H, Wahab S, Ahmed N, Fine J, Derynck J, Pu S, Ponce M, Marcon E, Zhang Z, Greenblatt JF, Pearlman RE, Lambert JP, Fillingham J (2021) Functional characterization of RebL1 highlights the evolutionary conservation of oncogenic activities of the RBBP4/7 orthologue in Tetrahymena thermophila. Nucleic acids research ( ):
  2. Ref:18178773: Smith JJ, Torigoe SE, Maxson J, Fish LC, Wiley EA (2008) A class II histone deacetylase acts on newly synthesized histones in Tetrahymena. Eukaryotic cell 7(3):471-82
  3. Ref:17715364: Parker K, Maxson J, Mooney A, Wiley EA (2007) Class I histone deacetylase Thd1p promotes global chromatin condensation in Tetrahymena thermophila. Eukaryotic cell 6(10):1913-24
  4. Ref:15879532: Wiley EA, Myers T, Parker K, Braun T, Yao MC (2005) Class I histone deacetylase Thd1p affects nuclear integrity in Tetrahymena thermophila. Eukaryotic cell 4(5):981-90
  5. Ref:11046129: Wiley EA, Ohba R, Yao MC, Allis CD (2000) Developmentally regulated rpd3p homolog specific to the transcriptionally active macronucleus of vegetative Tetrahymena thermophila. Molecular and cellular biology 20(22):8319-28

Sequences 

>TTHERM_00647330(coding)
ATGAATTAAAATACTCATACTTATGAAGAGAGTCCATCTTTTTAGGATTCTTTTATAGAT
TAACAATAATAAATTAAGAGTAATTTATCTCTTCATACATTGCAACCAAACGATACTTTT
CATGCTGAATACTAAACTCAGAATCCTCCCTCTTTTCAGGAAAAGAATCTATTTATTAAC
TAGTTAAATACCAATGAATACAGTCATGATAACTTTGAAGTTGATTAAGTAACAGGAGAG
TAAGACTATTTAAATGATGGTGAAAATAACACTAGAAATTATACTATAAATTACAATCAT
GTAGAAAATAATTAAGATGAAGATGATGAAATATACGCTGATTACTTGCCTGAAAATTTC
TTTGAGCAATTAAAACCGAACCCAAGCTAATTTACAATGAATTTTAATTAGAACACTAAA
AAAAAGGAAGAAAGAAAATCCATAAATAATCGTTCAACTTCTCTTAAAAAAAGAATAGAA
TAAAAAAATGATGAGATGTTATACACTAGAGCCGCCAAGTAGTAAATATGCCAAAACCTC
GTTTAGCAATAGATTGATAAAGAAATCAAATACATGTCTAATGCAAAAAAAATGAGCCAA
AATAGCTATAGAATAGTGGCAAAAAAATTAGAAAATGTAATATCTTCAAAAATGGCAGAA
ATTGACCCCTCTTTGAGTAAAATATTGACATTTTCTCAAGTAGGCAGATTATTCTAGCTG
CTTGATTTATTCACTGTAGTTAAATTTGATGAAAACTCATAAATCATCATCGAATAAAAT
ATAGATCCTCATAAAAAACTTAGATTTGCCCAAGAAACAATGTTTCACCAATAATTTTGG
TTTTTTGTTGTGAATTATATCAATAAAGAGACTGTATTGGGAGAAATAGTTTTTTCTACT
CTGAGAATATTGATGGATCCAATGCAATTAACGATTAAAGAAACTAAAGATTTCTTAGCT
TAGTATATGGGTAGGACACTTACATATTTAGAAGAAGATAGAAAAGCTGCTTAAGAGAGA
ACATAAGGATTTTAGCTCTGGAGCGTTGACGAAATTATCAAGGCATTTCGCTAGATTAAT
GAATAAAGAATTTTAAGGGAAAAGTCTTAAAACATAAATAATATTAATAATGTAAATAAA
AATAAAGCAAGCAATAAAGATTTAGATAAAAATATTTATACTTTCAAACCAACAATTGAT
AAAAACTCTTAATTAATTGATGAAATGCATAGGAAAAAATTTGCTATGGCATCTAGTGGA
CTAAATATCACTCCTATTAGACATTCAAATGCAACACAAAACACAAAGAGTATACAAAGT
ACTAATAAACTTATAAATTTACTCTAATCTTCCTAAAGTAACTAGAATCTGCATTCTTAA
GCTAGTATTTTACTCTAACAGTAAATGAATTAAACTCTGATCAATAATTAAAGCAATACA
AATAATTTGAATCACAACAGCAGCTCTTTGAGTTTTTTAAATAATACAAATAATTAAGTT
AATTAACACCCTAACATGAATTACAACGAAAATAATTCTACAACTACTAATGCTACAAGT
GACTATAGAAGCACTACTATGAATACTGATGTGGCTAATAATAGCTCTGAAAATTCATAA
AGAAACAGTGATAAATTTCTTTCTTAAAAAGTTAAATCTCTACTTTCTCAGCATTAGCAT
CCTTCTAGAACGAATTTAAATACAAACAATTAAAATTTTAACACTTTGTAGCACAATAGT
CATAATAATAACAACCAAAATGGCTAAATTGGAATGAGTGAATATGATCATGAAGATCCT
TAGAGCTACTTCTCAAAAATTAAAAGATGTGCTACATTAAGCAGTTTCTAAATTCCAGAG
GAAGATTATATAGAAGATATTAGCCACATTCCTTCAAATAATTTTGAAAAACCAATAGAT
AGAGTCAACATGATGCTATAGAAGTAGCAGCAAACCTAGAGAAAAATCAGTTAAAAAAAG
GAACAAATAATTTAGGAACAAATGAAGGAATGTACTTTTAAACCATAACTTCAAAGAAGT
GTTTCTCCTTAATACTCTGCATATTCTAGAAATCAAAAAGAATTATTACAAAGACAGTAG
CATGATATAAACAAAAGATAGTAAGCAGCAGAATAAAGAAATAGATAAAAGTAAGAATAT
GAAGAAAAACTATTAAATGAATGTACATTCTAACCTTCGTTATCTCCTTCTTAAAGGCTC
ATTAACAAAACAAGTAAATCACCAGTTTCACCTAAAGGGTTTGAATAAACTGTTCATAGA
ATGAGAGCTGCTTAGGATATTTAGCGTTAAAGCAGAAAAGCTTTTGATTAAGGATCATAT
AGCTCGAATGCCTAAATAGTAAGAAATTCTGTTGTTAGAAATTCATTAAGTCCAAATGTT
GAAATTAATAAATAAATTTAGATATAATAAAGCTTTTAGAAGCAAAATCCTTCTTAATAG
CAAGAACAATACTTATAATAAGTAAATTAACAAAAATAAATTTAATAAGATTTTTCAGAA
GATTTAGAAGGAGATATGCTACAATAATAACCATAATAAGAAAAATAATAATTTTACTAG
ATATATGAAAACTAAAATTAAAGTGCAAATCAAATGCATTAATAAAAATAAAGCATAGTT
GAAAAAGAAAACGAACAAAGATTAGATTTAGAAAACTAATTCATTAATGACTAATAAAGT
TTTGTAGAGAAAATAACAAATAAATAAAAACCTAATTTTTAAGATTAAACATTTGGAGAT
TCATAAAAGAAAAACCAATAATTAGAAATTTATTAAAAACCAAAAGCTAAGCCATTTTTT
GTGCTAGATATTATTACACCAAAAGGAGAGAAAGCAGCTTTAGAACTATTTAAGGATGAT
GATATTTACCAAAAAGCTGAAGTATTCTCTTAACAATATTAATTAGACGAAATTACATAA
AGTAAAATAGAATAGATCCTAGATTAGCAAATGCAAAAATATCTCTTAAAAAAATAAAAC
ACTGTAAAAAATAATTGA



>TTHERM_00647330(gene)
AGAAAACAACTAATTAAGAAAGTAAAGTAATCAATATTGAATTAAAATTTAACTAGGAGA
CTCAAGATTGTGCTTGATCTAAAGCAGACTCCAGATGAATTAAAATACTCATACTTATGA
AGAGAGTCCATCTTTTTAGGATTCTTTTATAGATTAACAATAATAAATTAAGAGTAATTT
ATCTCTTCATACATTGCAACCAAACGATACTTTTCATGCTGAATACTAAACTCAGAATCC
TCCCTCTTTTCAGGAAAAGAATCTATTTATTAACTAGTTAAATACCAATGAATACAGTCA
TGATAACTTTGAAGTTGATTAAGTAACAGGAGAGTAAGACTATTTAAATGATGGTGAAAA
TAACACTAGAAATTATACTATAAATTACAATCATGTAGAAAATAATTAAGATGAAGATGA
TGAAATATACGCTGATTACTTGCCTGAAAATTTCTTTGAGCAATTAAAACCGAACCCAAG
CTAATTTACAATGAATTTTAATTAGAACACTAAAAAAAAGGAAGAAAGAAAATCCATAAA
TAATCGTTCAACTTCTCTTAAAAAAAGAATAGAATAAAAAAATGATGAGATGTTATACAC
TAGAGCCGCCAAGTAGTAAATATGCCAAAACCTCGTTTAGCAATAGATTGATAAAGAAAT
CAAATACATGTCTAATGCAAAAAAAATGAGCCAAAATAGCTATAGAATAGTGGCAAAAAA
ATTAGAAAATGTAATATCTTCAAAAATGGCAGAAATTGACCCCTCTTTGAGTAAAATATT
GACATTTTCTCAAGTAGGCAGATTATTCTAGCTGCTTGATTTATTCACTGTAGTTAAATT
TGATGAAAACTCATAAATCATCATCGAATAAAATATAGATCCTCATAAAAAACTTAGATT
TGCCCAAGAAACAATGTTTCACCAATAATTTTGGTTTTTTGTTGTGAATTATATCAATAA
AGAGACTGTATTGGGAGAAATAGTTTTTTCTACTCTGAGAATATTGATGGATCCAATGCA
ATTAACGATTAAAGAAACTAAAGATTTCTTAGCTTAGTATATGGGTAGGACACTTACATA
TTTAGAAGAAGATAGAAAAGCTGCTTAAGAGAGAACATAAGGATTTTAGCTCTGGAGCGT
TGACGAAATTATCAAGGCATTTCGCTAGATTAATGAATAAAGAATTTTAAGGGAAAAGTC
TTAAAACATAAATAATATTAATAATGTAAATAAAAATAAAGCAAGCAATAAAGATTTAGA
TAAAAATATTTATACTTTCAAACCAACAATTGATAAAAACTCTTAATTAATTGATGAAAT
GCATAGGAAAAAATTTGCTATGGCATCTAGTGGACTAAATATCACTCCTATTAGACATTC
AAATGCAACACAAAACACAAAGAGTATACAAAGTACTAATAAACTTATAAATTTACTCTA
ATCTTCCTAAAGTAACTAGAATCTGCATTCTTAAGCTAGTATTTTACTCTAACAGTAAAT
GAATTAAACTCTGATCAATAATTAAAGCAATACAAATAATTTGAATCACAACAGCAGCTC
TTTGAGTTTTTTAAATAATACAAATAATTAAGTTAATTAACACCCTAACATGAATTACAA
CGAAAATAATTCTACAACTACTAATGCTACAAGTGACTATAGAAGCACTACTATGAATAC
TGATGTGGCTAATAATAGCTCTGAAAATTCATAAAGAAACAGTGATAAATTTCTTTCTTA
AAAAGTTAAATCTCTACTTTCTCAGCATTAGCATCCTTCTAGAACGAATTTAAATACAAA
CAATTAAAATTTTAACACTTTGTAGCACAATAGTCATAATAATAACAACCAAAATGGCTA
AATTGGAATGAGTGAATATGATCATGAAGATCCTTAGAGCTACTTCTCAAAAATTAAAAG
ATGTGCTACATTAAGCAGTTTCTAAATTCCAGAGGAAGATTATATAGAAGATATTAGCCA
CATTCCTTCAAATAATTTTGAAAAACCAATAGATAGAGTCAACATGATGCTATAGAAGTA
GCAGCAAACCTAGAGAAAAATCAGTTAAAAAAAGGAACAAATAATTTAGGAACAAATGAA
GGAATGTACTTTTAAACCATAACTTCAAAGAAGTGTTTCTCCTTAATACTCTGCATATTC
TAGAAATCAAAAAGAATTATTACAAAGACAGTAGCATGATATAAACAAAAGATAGTAAGC
AGCAGAATAAAGAAATAGATAAAAGTAAGAATATGAAGAAAAACTATTAAATGAATGTAC
ATTCTAACCTTCGTTATCTCCTTCTTAAAGGCTCATTAACAAAACAAGTAAATCACCAGT
TTCACCTAAAGGGTTTGAATAAACTGTTCATAGAATGAGAGCTGCTTAGGATATTTAGCG
TTAAAGCAGAAAAGCTTTTGATTAAGGATCATATAGCTCGAATGCCTAAATAGTAAGAAA
TTCTGTTGTTAGAAATTCATTAAGTCCAAATGTTGAAATTAATAAATAAATTTAGATATA
ATAAAGCTTTTAGAAGCAAAATCCTTCTTAATAGCAAGAACAATACTTATAATAAGTAAA
TTAACAAAAATAAATTTAATAAGATTTTTCAGAAGATTTAGAAGGAGATATGCTACAATA
ATAACCATAATAAGAAAAATAATAATTTTACTAGATATATGAAAACTAAAATTAAAGTGC
AAATCAAATGCATTAATAAAAATAAAGCATAGTTGAAAAAGAAAACGAACAAAGATTAGA
TTTAGAAAACTAATTCATTAATGACTAATAAAGTTTTGTAGAGAAAATAACAAATAAATA
AAAACCTAATTTTTAAGATTAAACATTTGGAGATTCATAAAAGAAAAACCAATAATTAGA
AATTTATTAAAAACCAAAAGCTAAGCCATTTTTTGTGCTAGATATTATTACACCAAAAGG
AGAGAAAGCAGCTTTAGAACTATTTAAGGATGATGATATTTACCAAAAAGCTGAAGTATT
CTCTTAACAATATTAATTAGACGAAATTACATAAAGTAAAATAGAATAGATCCTAGATTA
GCAAATGCAAAAATATCTCTTAAAAAAATAAAACACTGTAAAAAATAATTGATTTTAAAC
CAAATAACATACTATTTTCAACCAAATTGATAATTAAGAAATCTGTAAATTATTAAAATA
TTAAATAAATGTTTAACAAAATAATTAAAAAATTATATATATTATAACAATAAATAAATA
AATCAATAAAATAAACTTTCAA



>TTHERM_00647330(protein)
MNQNTHTYEESPSFQDSFIDQQQQIKSNLSLHTLQPNDTFHAEYQTQNPPSFQEKNLFIN
QLNTNEYSHDNFEVDQVTGEQDYLNDGENNTRNYTINYNHVENNQDEDDEIYADYLPENF
FEQLKPNPSQFTMNFNQNTKKKEERKSINNRSTSLKKRIEQKNDEMLYTRAAKQQICQNL
VQQQIDKEIKYMSNAKKMSQNSYRIVAKKLENVISSKMAEIDPSLSKILTFSQVGRLFQL
LDLFTVVKFDENSQIIIEQNIDPHKKLRFAQETMFHQQFWFFVVNYINKETVLGEIVFST
LRILMDPMQLTIKETKDFLAQYMGRTLTYLEEDRKAAQERTQGFQLWSVDEIIKAFRQIN
EQRILREKSQNINNINNVNKNKASNKDLDKNIYTFKPTIDKNSQLIDEMHRKKFAMASSG
LNITPIRHSNATQNTKSIQSTNKLINLLQSSQSNQNLHSQASILLQQQMNQTLINNQSNT
NNLNHNSSSLSFLNNTNNQVNQHPNMNYNENNSTTTNATSDYRSTTMNTDVANNSSENSQ
RNSDKFLSQKVKSLLSQHQHPSRTNLNTNNQNFNTLQHNSHNNNNQNGQIGMSEYDHEDP
QSYFSKIKRCATLSSFQIPEEDYIEDISHIPSNNFEKPIDRVNMMLQKQQQTQRKISQKK
EQIIQEQMKECTFKPQLQRSVSPQYSAYSRNQKELLQRQQHDINKRQQAAEQRNRQKQEY
EEKLLNECTFQPSLSPSQRLINKTSKSPVSPKGFEQTVHRMRAAQDIQRQSRKAFDQGSY
SSNAQIVRNSVVRNSLSPNVEINKQIQIQQSFQKQNPSQQQEQYLQQVNQQKQIQQDFSE
DLEGDMLQQQPQQEKQQFYQIYENQNQSANQMHQQKQSIVEKENEQRLDLENQFINDQQS
FVEKITNKQKPNFQDQTFGDSQKKNQQLEIYQKPKAKPFFVLDIITPKGEKAALELFKDD
DIYQKAEVFSQQYQLDEITQSKIEQILDQQMQKYLLKKQNTVKNN