Identifiers and Description

Gene Model Identifier

TTHERM_00684560

Standard Name

ORC2 (origin recognition complex subunit 2)

Aliases

PreTt18169 | 104.m00101 | 3732.m00015

Description

ORC2 origin recognition complex subunit 2; Origin recognition complex, subunit 2

Genome Browser (Macronucleus)

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Genome Browser (Micronucleus)

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External Links

Gene Ontology Annotations

No Data fetched for Gene Ontology Annotations

Domains

  • ( PF04084 ) Origin recognition complex subunit 2

Gene Expression Profile

Vegetative Cell Cycle (Zhang et al., 2023)

GeneMania

Tetrahymena Stock Center

No Data fetched for Tetrahymena Stock Center

Homologs

No Data fetched for Homologs

General Information

No. Gene Name(s) Paragraph Text
2272 ORC2 Gene Information: TTHERM_00684560 against human ORC subunit 2 protein e-value= 8e-16 Reverse BLAST of NP_006181.1 against TGD: TTHERM_00684560 hypothetical protein e-value= 8e-15.
2273 ORC2 The conserved Origin Recognition Complex (ORC) determines the sites for replication initiation in eukaryotic chromosomes and serves as a scaffold for pre-replicative complex (pre-RC) assembly. Although ORC subunits are conserved in eukaryotes, the cis-acting DNA sequence requirements for replicator function are not.
2274 ORC2 Nucleic Acid Interactions: Orc2p bound to streptavidin sepharose in 26T RNA aptamer-tagged strains (TD152 and MM202, respectively)
2275 ORC2 Protein Interaction Partners: Tetrahymena thermophila ORC physically associates with Orc1p in western blotting (native gel electrophoresis and immunoprecipitation analyses. Western blotting was similarly used to monitor the migration of nuclease-treated ORC complexes under native EMSA gel conditions. The mobility of Orc1p and Orc2p increased following MNase and RNase A treatment, but was unaltered by DNase I (Figure 3D). Orc1p and Orc2p co-migrated under all conditions, suggesting that they remain associated after the RNA is destroyed.
2276 ORC2 Biochemical Activities: Consistent with previous analyses of Orc2p/Tt-p69 and histone H3 (Mohammad et al, 2003), ∼50% of Orc2 was rendered soluble by DNase I. TtORC also crossreacts with a rabbit polyclonal antibodies raised against Xenopus laevis / Orc2p.
2277 ORC2 Regulation & Expression: Orc2p crossreactive subunit, Tt-p69, localizes to the macronucleus during vegetative S phase. Cell cycle regulated Maximal protein expression at G1/S border

Associated Literature

  1. Ref:18007594: Mohammad MM, Donti TR, Sebastian Yakisich J, Smith AG, Kapler GM (2007) Tetrahymena ORC contains a ribosomal RNA fragment that participates in rDNA origin recognition. The EMBO journal 26(24):5048-60
  2. Ref:12832485: Mohammad M, York RD, Hommel J, Kapler GM (2003) Characterization of a novel origin recognition complex-like complex: implications for DNA recognition, cell cycle control, and locus-specific gene amplification. Molecular and cellular biology 23(14):5005-17

Sequences 

>TTHERM_00684560(coding)
ATGCAAAATAATTAACAGGAAAGATTGGAATTCTAGGAAAAATATGGTATTTAAAAGGAC
TATATTTCTTTATCCAAAGAAGATAAAAAGAAATAAGCTGCAGATGAAGTCATTGAGTTT
AACAATAAAAACTTGATTTCTTATTTAGAAGATAAATATTAAGTAGAAGACACTGAAAAA
GTAAAAGAAGACAAAGCTAAAGCAATTGTGAAATCATGTTTTATGATACAATCAGGATTT
AATGTTTGCTTACATGGTTATGGCTCTAAGAGACAAATTATTTAAGAAATATCAGATTAA
TTTATTAATTACCCTAAATTAATCATTTAAGGATATTCAGCAGCTACTTAAATATAGCAA
ATTCTATAAAAACTAAAGTACATTGTGGTAAAGATTACTGGGTAAAACTCTGGTTAAAAG
AGAATTGAAGGAATATTAGAGTATCTAAAATAATAAGAGGAAAATATGGGAAAAATTTCC
TCTTAAGTACCTTTTATTTTAATTCTATTTCATAATATAGATTCTAAATAATTTATTGAG
TAAGATTCTGTCAAAATTTTATCTGCTGTACTAAATTTACCATATATAAAAGCTGTTTGT
AGTATTGATCATGTGAAGTATCCAATTATTCTGACTTCTAAAGTTTTAGAGTCATTTAAT
TTTAGTTTCATAGAAGTCCATACTCATTAATGCTATCTACATGAATTCAAAGTTTCCTAA
AATACATTTAACTTAAAAAAAGAAAAAGAAGGAGAATCTCTTAAATATATTTTTAGAAGT
CTGACAAAAGTTTAAAAAGAAATAATATAGTTTGTTGCAAAATATATTTAAGATAAATTA
GATTAAAATCCAGGAACAGCTATTTAAATAAATGGATTAACTGAAGATGGATAGATTATT
GGTTTAACTTTAAAAGAGTTATTAGAAATGCTAATTGATGAAATGATTTTGAACAGTGAG
TAGCAGTTAAAAGAAAATTTACTTGAAATACTTGATCACAAAGTTATAATTTCAATAGAA
AACTAGTAAAAAACTTACTATACAATGAATTTCAGCAGACAAATTTTAGAAAAAATTGTC
AACAACAAGTTGGAGGATGGAGAAGAAGATTAGGAGCAGGAAGAAGAGGAAGAAAACAAT
TCAGTAAAAGATATGGAAGAAGAGAATTAAATAATATCTAACAGCTAAAACAGTTCTCAA
AGTGAAGATGAAGATGTTAAAGGTAAAAAAAAATATAGATAAACAAGAAGAGAAGGTAAA
AAGAAAAAAAATAGTAGTGAGAGCTTATCAGATTCAGAAAATGAAAAACATAAGTTTAAT
AAGAAAACTTCTGGAATTTTGAAAACTAAAAATAAGTAATAAAAGAATAAAAAAAGTAGC
TTTACTTCATAAAGTAGTGAAGAAGATGGTGATGATGATAGCTATTAAAATTGA



>TTHERM_00684560(gene)
CTATTTTCGTAAAGACACATATTTTTTAGGATAAAAGAGTTTATAAATACATTAAACTTC
ATGCAAAATAATTAACAGGAAAGATTGGAATTCTAGGAAAAATATGGTATTTAAAAGGAC
TATATTTCTTTATCCAAAGAAGATAAAAAGAAATAAGCTGCAGATGAAGTCATTGAGTTT
AACAATAAAAACTTGATTTCTTATTTAGAAGATAAATATTAAGTAGAAGACACTGAAAAA
GTAAAAGAAGACAAAGCTAAAGCAATTGTGAAATCATGTTTTATGATACAATCAGGATTT
AATGTTTGCTTACATGGTTATGGCTCTAAGAGACAAATTATTTAAGAAATATCAGATTAA
TTTATTAATTACCCTAAATTAATCATTTAAGGATATTCAGCAGCTACTTAAATATAGCAA
ATTCTATAAAAACTAAAGTACATTGTGGTAAAGATTACTGGGTAAAACTCTGGTTAAAAG
AGAATTGAAGGAATATTAGAGTATCTAAAATAATAAGAGGAAAATATGGGAAAAATTTCC
TCTTAAGTACCTTTTATTTTAATTCTATTTCATAATATAGATTCTAAATAATTTATTGAG
TAAGATTCTGTCAAAATTTTATCTGCTGTACTAAATTTACCATATATAAAAGCTGTTTGT
AGTATTGATCATGTGAAGTATCCAATTATTCTGACTTCTAAAGTTTTAGAGTCATTTAAT
TTTAGTTTCATAGAAGTCCATACTCATTAATGCTATCTACATGAATTCAAAGTTTCCTAA
AATACATTTAACTTAAAAAAAGAAAAAGAAGGAGAATCTCTTAAATATATTTTTAGAAGT
CTGACAAAAGTTTAAAAAGAAATAATATAGTTTGTTGCAAAATATATTTAAGATAAATTA
GATTAAAATCCAGGAACAGCTATTTAAATAAATGGATTAACTGAAGATGGATAGATTATT
GGTTTAACTTTAAAAGAGTTATTAGAAATGCTAATTGATGAAATGATTTTGAACAGTGAG
TAGCAGTTAAAAGAAAATTTACTTGAAATACTTGATCACAAAGTTATAATTTCAATAGAA
AACTAGTAAAAAACTTACTATACAATGAATTTCAGCAGACAAATTTTAGAAAAAATTGTC
AACAACAAGTTGGAGGATGGAGAAGAAGATTAGGAGCAGGAAGAAGAGGAAGAAAACAAT
TCAGTAAAAGATATGGAAGAAGAGAATTAAATAATATCTAACAGCTAAAACAGTTCTCAA
AGTGAAGATGAAGATGTTAAAGGTAAAAAAAAATATAGATAAACAAGAAGAGAAGGTAAA
AAGAAAAAAAATAGTAGTGAGAGCTTATCAGATTCAGAAAATGAAAAACATAAGTTTAAT
AAGAAAACTTCTGGAATTTTGAAAACTAAAAATAAGTAATAAAAGAATAAAAAAAGTAGC
TTTACTTCATAAAGTAGTGAAGAAGATGGTGATGATGATAGCTATTAAAATTGATAAAAA
AAGCTAGAATAAAATAATTATAAAAATAATTTTTAAAAGCAATAATATTTTGCAATATTA
AAAGAGGTAGATAAATAAATAAATATATATATAGAAAGAAAATATTTTTTAAAATGAATG
AACTTAAAATATAATGCTTATTAATAAATATAGAAATATAAATTAAACGAATGTTTGAGC
CTAATTAAGTTGTTAGTTGATTATTAATGTGA



>TTHERM_00684560(protein)
MQNNQQERLEFQEKYGIQKDYISLSKEDKKKQAADEVIEFNNKNLISYLEDKYQVEDTEK
VKEDKAKAIVKSCFMIQSGFNVCLHGYGSKRQIIQEISDQFINYPKLIIQGYSAATQIQQ
ILQKLKYIVVKITGQNSGQKRIEGILEYLKQQEENMGKISSQVPFILILFHNIDSKQFIE
QDSVKILSAVLNLPYIKAVCSIDHVKYPIILTSKVLESFNFSFIEVHTHQCYLHEFKVSQ
NTFNLKKEKEGESLKYIFRSLTKVQKEIIQFVAKYIQDKLDQNPGTAIQINGLTEDGQII
GLTLKELLEMLIDEMILNSEQQLKENLLEILDHKVIISIENQQKTYYTMNFSRQILEKIV
NNKLEDGEEDQEQEEEEENNSVKDMEEENQIISNSQNSSQSEDEDVKGKKKYRQTRREGK
KKKNSSESLSDSENEKHKFNKKTSGILKTKNKQQKNKKSSFTSQSSEEDGDDDSYQN