Identifiers and Description

Gene Model Identifier

TTHERM_00853150

Standard Name

RDR1 (RNA-Dependent RNA polymerase 1)

Aliases

PreTt01214 | 150.m00106 | 3834.m00380

Description

RDR1 RNA-dependent RNA polymerase RdRP2; RNA-Dependent RNA polymerase; associated with RNA silencing; RNA-dependent RNA polymerase, eukaryotic-type

Genome Browser (Macronucleus)

Full Screen View

No Genome Browser Data Present

Genome Browser (Micronucleus)

Full Screen View

No Genome Browser Data Present

External Links

• Uniprot
• Alphafold

Gene Ontology Annotations

Cellular Component

• cytoplasm (IDA) | GO:0005737
• nuclear RNA-directed RNA polymerase complex (IDA) | GO:0031380
• RNA-directed RNA polymerase complex (IDA) | GO:0031379

Domains

• ( PF05183 ) RNA dependent RNA polymerase

Gene Expression Profile

Vegetative Cell Cycle (Zhang et al., 2023)

GeneMania

No results were calculated for this gene in GeneMania.

Tetrahymena Stock Center

• ( SD02508 ) Micronucleus: neo2 into RDR1
• ( SD02509 ) Micronucleus: neo2 into RDR1
• ( SD02510 ) Micronucleus: neo2 into RDR1
• ( SD02511 ) Micronucleus: neo2 into RDR1
• ( SD02512 ) Macronucleus: HA-FLAG tag of RDR1
• ( SD02513 ) Macronucleus: HA-FLAG tagged RDR1 with neo3 in flank
• ( SD02514 ) Macronucleus: HA-FLAG tagged RDR1 with neo3 in flank
• ( SD02515 ) Macronucleus: HA-FLAG tagged RDR1 with neo3 in flank

Homologs

No Data fetched for Homologs

General Information

No Data fetched for General Information

Associated Literature

1. Ref:22706992: Talsky KB, Collins K (2012) Strand-asymmetric endogenous Tetrahymena small RNA production requires a previously uncharacterized uridylyltransferase protein partner. RNA (New York, N.Y.) 18(8):1553-62
2. Ref:20622019: Talsky KB, Collins K (2010) Initiation by a eukaryotic RNA-dependent RNA polymerase requires looping of the template end and is influenced by the template-tailing activity of an associated uridyltransferase. The Journal of biological chemistry 285(36):27614-23
3. Ref:19451546: Lee SR, Talsky KB, Collins K (2009) A single RNA-dependent RNA polymerase assembles with mutually exclusive nucleotidyl transferase subunits to direct different pathways of small RNA biogenesis. RNA (New York, N.Y.) 15(7):1363-74
4. Ref:19656801: Couvillion MT, Lee SR, Hogstad B, Malone CD, Tonkin LA, Sachidanandam R, Hannon GJ, Collins K (2009) Sequence, biogenesis, and function of diverse small RNA classes bound to the Piwi family proteins of Tetrahymena thermophila. Genes & development 23(17):2016-32
5. Ref:17603500: Lee SR, Collins K (2007) Physical and functional coupling of RNA-dependent RNA polymerase and Dicer in the biogenesis of endogenous siRNAs. Nature structural & molecular biology 14(7):604-10

Sequences

>TTHERM_00853150(coding)
ATGATTGAACAATCTATAGAAGAGGAAAAAATCATATGCTGTATAGGCTATATAAATGGA
AAGATAACTTCTAAAAACTTTACTACCTCATTATGCTTTGCATCAATATAAAAATATCTT
TAAGAGCAACAACATAGATTTAAAAGCATTAAATGTGTCTCTATTTATACTCCAAAAAAG
GAGCAATAGTAGATTGGTTTAGATACCATTGATGATGTTAAGGAAAAAATAAAAGAGATA
CAGAAATCTATTGATCATAATTATTTGAATATACCTCAACACCTGAAAGAAGAGAGATAT
AGAGTAAACTATAAAAGCTTAAATTTTTAGGAAGGTTAATAGTCTTTGGATAATGATGAT
GATAGCAATTTACTTGGTAGTGAATATTAGTTTTTAAAAAACTTTATTACAAAAGAAAAT
GATGATATTCATCTGAAGGAAGAAATGGCTAGCTTTAATAAAATAGAAAGCTTTTGTTAA
AATGTATTAAAGAACTCTAAAAATGAGTCTAAAATAAATACTGTAAACTAATAAAAAGAT
TAGTATGCAAGGATAAATAGCTGGATTATAATTTTAAGTGGAACGGAATAGGATTTAGAA
GTTCTGAACGATTCAAAGATTGATGTAAAGACAAAACATGAAAAATATTACTTCTTTTTA
AAAACAAAACATAATGACAACGAAAAAAAAAATTATTTGAAAATTTGTACTGATGAATTA
TCGAATTATGCTAAGGAATAGATGAATTTTGTAAATATAAAATTGCTTCAAGTTAGTTTA
GGGATATTTGAATTATACGGCGGTGCTTTATACTACAATTGTTAGTTTTAATTTGAAGAA
AACATAGACTATGTAAACACAATCAGCATCTATATGTAAAATGATGAATCAGAGAGCAAG
TAAAAATAGTAATCGGTATCTATTGTGTTAGCATATAATGATTATTAGCTAAAAATTGAG
TTTCCATATTTATATTGCAATGAAATAAGATTTGATTAAAGAGGTAAAAATATGTATTAC
TTTTAATTAGTTGTTCCTCCTAAATATTTTGTCAGAGAAAATTCAGCTAATATTTAAGAA
AACTTGGATTAAAAAAAGAACACTTGGATTAGAGTAGATAACTTCTTTGCTCATGACATC
AGTAATGAGCTTAAAAGAATAGAATTTGTAAACAATACAGTTATTTGTATTGAGATAGAA
AATGAAACAGTCAATACATTCTAAACGATACTTAAAAATTCTTTTTCAAACGTGTTTAAA
GATACAGACCCTATTCCTATTTCAAATGTGTCACAACATTTTCCTTAAGAACCTGAAAAT
GATTTAGACTAATAATGGGAAAGACTTCTAAATAGATATTCATTCAATTTAAGCTATTCG
ATCTTAACACTAATATCTCACAATAAACTAAGTGTCGTCAAATTAAGAGAATTTCTTGAC
TATATAGATCTTTAAATAAGAGATGGTAAATCAGAGTATATATTAGAAAAAGGAATTTAG
TATTATTACAACTAAAAGAAAGAAATTAATCGCCTCAATTTTACAGCTTTCTAAATACTT
TTCTAGGACGTGTAAAGATAAGAAAATTACTTCAAAATTTAAGAGATCAGAGCTAAAAGA
GATGTCAAAGTTATCGCAACAAGAAGGGTAAGTATTACTCCTAGTGGATTGATTTATAAC
TTAAAAGAATTAGAACTGTCAAATAGAGTTTTGAGAATTTTTTAAGACTCAACTGATTCA
TTTATAAGAGGAACATTTTAAGATGATTCCTTAGAAAATGCAAAGCATATGAAGTATGTC
TCAAGATATTTCAGGTAAAAGCTTAAATCCTTTAAAGTTTTAAACTGGGAATTTATTAAT
TTTGGATGGAGTGCTTCTCAACTTAGGAACTCATCATCTTGGTTCTTTCATGAAACTGAA
GTTCTCAAAAAATAAGATGTAATTACAAGAATTGGTGATTTTTCAGGTATTAATCCTCCA
GCTAAAAAATCTGCTAGAATTGGATAATGTTTTAGCTCAAGTACTCCCTATATCTTGAAA
GAAAGACCTAACATAGAATTCATTGATGATATTACTACACCCTCAGCAGTTTAAGAAGTT
GATGCTAATGGAAATATTTGCTACTTTAGATAAAAGAAAAAAGTTTTTACAGATGGTATT
GGAACTATTTCATAAGATCTTATAAGAAAAATATCAGATCATCTTAATGCCTTTTTTATG
TGTGCTATATAAGTAAGATATGGAGGAGCTAAAGGAGTGTTGACAATTGATTATAAAAGT
GATAAAAAAAATACAATATAGTTGAGAGAAAGTATGATAAAATATAAAATTACAGATGAA
TAAGACGAATTAATAATTGATATTCTAGATAGAAACAAATATAGATTTGGATATTTGAAT
AGGTAAGTCATTATCTTACTTAGATCACTAGGTATTGAAAACGAATAATTCATCTAAGCT
CAGCAAGAATACATAGAAGAGTTAAATAAGTCAGCATTGAAAGATGCAAGTATATTTACA
CTTTTTAACACAGATTATTCAAAGGATGGAACAAGATTATTTCCAATCACCCATCTTCTA
AGAAGTATGTAGACAAGTAGGATTGATATGGCCTAAGATATATTCTGTAAAGGAGTTATT
CATACCCTCAAAAAAAGAGGTTTTTATATTCTGAGAAAGAAGAGTAACATTTTTCTTAAA
AAGAGTGCAAGATTGATGGGTGTTATAGATGAATATAATGTGTTAGAACAAGATGAAGTT
TTTGCATATATAAGTTAGGATTCAGAAAAATCTTTCTTTTAAGTTGAGGGAGATGTAATT
GTAGTTAAAAACCCATGTTTACATCCTGGTGATATTAGAAAATTAAAAGCTGTTACTTTG
GAAACTATTTCGTAAAGATTAGGAGAAGGAAAAATAAATCCTTTTAAAAAATTAAGGAAC
ATCCTAATATTCCCTTAAAAAGGTAAAGAGCCTATCACTTCATAAATAGCAGGTTCCGAT
TTGGATGGTGATTGCTATTTTATTTGCTGGGATTAAAGATTTATTCCCTTGGAATAGAAA
GAACCTTTTAACTATGATGAAGATGATAATAGTTAAAAAAAATAAATCTCATAAGAACAG
TATGATATAGAAATCTCTAAAAAGCCTAATACTTAGTTTAAAACTAAAAATCCTGTACGA
GAAGTGATTGATGATAATGAATAAATGCTAGACTTTTTTATAGATTTTATAAATAATGAA
AATCTTGGTAAAATAGATAATTCTCATTTAGTCTTTGCAGATAAAAGTCCATTATACGCT
GAAGACCCTAAATGTTTAAAGTTGGCATCACTACATGCATAAGCTGTAGATTATGCAAAA
ACAGGTATTTCTCCTTAATTACCAAAAAACCTTGCAATAAAAGAGTACCCTGATTTTATG
GAAAAAGATGACAAACCATCTTATTAATCATAAAGCATTTTAGGTATTTTATATAGACAA
GTAAAAGACCAGTATGAAAAACACTGTAAAAACGATATAGAAGGAGATAGATCTTAAACT
TTTATTGATAATAATTTAATATTCAATCCGCCTACATTTGTTAACGAAATTGTAGATGAG
TAAGAAAATAAAACTTATCTTGAATAGGAAATTGATTTCGCTATTGAAGCAAATAATCAA
TGGAATATAGAAATAAATAATCTCAAGCAATTCTTTGGTGCTCAAAATGAGTTTGAAATT
TACACAGGTAATTTTTCCAAACTCATAAAAGTAGAGAGGAAAAAAAAGATGAGTGCAGAA
ATAGTATAGCAAAGAATCTTGTCTACATTATTAGCAATAAAAAATAGGTTTGAGCTTTTG
TTCTTTAAAAATTTAGATGAAGATAGAGATAGATTTTATGATGAAACAAACAGAAAAAAA
TATTATAGTAAAAAATGTAAAATAAGAGCTACTTTATGGTATATCATATGCTACTTAAGA
AAACCTGAACATATTGTTTAATATGCAGTAAACTTTGAGAAAGATTCTTTACTAGGACAG
ACTTTCACTTTTTTGAGTCAATAAAAATATTTTGGAGAAACAATCATAAGAGGAAATAGA
AATCCAAAAACTATTCAATTCTTCAATGAAAATAATGATTATGTTGGCTTATCATGGTTT
ATAATTAGTAATGTCCTGTTAGAAATAAAGAAAGATAATGATAACTATAAAATGAATGAA
TAAAAATAATAAAAAAGTGAATAATAAATAAAGGAATTTGAAGATTATAAAAAAAAATTC
TATTAAAATGATTTAAATGGATACCATTCTGACGAATATTTATGA

>TTHERM_00853150(gene)
TAATAATTAAACAATTCAATAATTTAACAAGTTTAGATATTAGATAAAAAATAGATAAGT
TAAAATGATTGAACAATCTATAGAAGAGGAAAAAATCATATGCTGTATAGGCTATATAAA
TGGAAAGATAACTTCTAAAAACTTTACTACCTCATTATGCTTTGCATCAATATAAAAATA
TCTTTAAGAGCAACAACATAGATTTAAAAGCATTAAATGTGTCTCTATTTATACTCCAAA
AAAGGAGCAATAGTAGATTGGTTTAGATACCATTGATGATGTTAAGGAAAAAATAAAAGA
GATACAGAAATCTATTGATCATAATTATTTGAATATACCTCAACACCTGAAAGAAGAGAG
ATATAGAGTAAACTATAAAAGCTTAAATTTTTAGGAAGGTTAATAGTCTTTGGATAATGA
TGATGATAGCAATTTACTTGGTAGTGAATATTAGTTTTTAAAAAACTTTATTACAAAAGA
AAATGATGATATTCATCTGAAGGAAGAAATGGCTAGCTTTAATAAAATAGAAAGCTTTTG
TTAAAATGTATTAAAGAACTCTAAAAATGAGTCTAAAATAAATACTGTAAACTAATAAAA
AGATTAGTATGCAAGGATAAATAGCTGGATTATAATTTTAAGTGGAACGGAATAGGATTT
AGAAGTTCTGAACGATTCAAAGATTGATGTAAAGACAAAACATGAAAAATATTACTTCTT
TTTAAAAACAAAACATAATGACAACGAAAAAAAAAATTATTTGAAAATTTGTACTGATGA
ATTATCGAATTATGCTAAGGAATAGATGAATTTTGTAAATATAAAATTGCTTCAAGTTAG
TTTAGGGATATTTGAATTATACGGCGGTGCTTTATACTACAATTGTTAGTTTTAATTTGA
AGAAAACATAGACTATGTAAACACAATCAGCATCTATATGTAAAATGATGAATCAGAGAG
CAAGTAAAAATAGTAATCGGTATCTATTGTGTTAGCATATAATGATTATTAGCTAAAAAT
TGAGTTTCCATATTTATATTGCAATGAAATAAGATTTGATTAAAGAGGTAAAAATATGTA
TTACTTTTAATTAGTTGTTCCTCCTAAATATTTTGTCAGAGAAAATTCAGCTAATATTTA
AGAAAACTTGGATTAAAAAAAGAACACTTGGATTAGAGTAGATAACTTCTTTGCTCATGA
CATCAGTAATGAGCTTAAAAGAATAGAATTTGTAAACAATACAGTTATTTGTATTGAGAT
AGAAAATGAAACAGTCAATACATTCTAAACGATACTTAAAAATTCTTTTTCAAACGTGTT
TAAAGATACAGACCCTATTCCTATTTCAAATGTGTCACAACATTTTCCTTAAGAACCTGA
AAATGATTTAGACTAATAATGGGAAAGACTTCTAAATAGATATTCATTCAATTTAAGCTA
TTCGATCTTAACACTAATATCTCACAATAAACTAAGTGTCGTCAAATTAAGAGAATTTCT
TGACTATATAGATCTTTAAATAAGAGATGGTAAATCAGAGTATATATTAGAAAAAGGAAT
TTAGTATTATTACAACTAAAAGAAAGAAATTAATCGCCTCAATTTTACAGCTTTCTAAAT
ACTTTTCTAGGACGTGTAAAGATAAGAAAATTACTTCAAAATTTAAGAGATCAGAGCTAA
AAGAGATGTCAAAGTTATCGCAACAAGAAGGGTAAGTATTACTCCTAGTGGATTGATTTA
TAACTTAAAAGAATTAGAACTGTCAAATAGAGTTTTGAGAATTTTTTAAGACTCAACTGA
TTCATTTATAAGAGGAACATTTTAAGATGATTCCTTAGAAAATGCAAAGCATATGAAGTA
TGTCTCAAGATATTTCAGGTAAAAGCTTAAATCCTTTAAAGTTTTAAACTGGGAATTTAT
TAATTTTGGATGGAGTGCTTCTCAACTTAGGAACTCATCATCTTGGTTCTTTCATGAAAC
TGAAGTTCTCAAAAAATAAGATGTAATTACAAGAATTGGTGATTTTTCAGGTATTAATCC
TCCAGCTAAAAAATCTGCTAGAATTGGATAATGTTTTAGCTCAAGTACTCCCTATATCTT
GAAAGAAAGACCTAACATAGAATTCATTGATGATATTACTACACCCTCAGCAGTTTAAGA
AGTTGATGCTAATGGAAATATTTGCTACTTTAGATAAAAGAAAAAAGTTTTTACAGATGG
TATTGGAACTATTTCATAAGATCTTATAAGAAAAATATCAGATCATCTTAATGCCTTTTT
TATGTGTGCTATATAAGTAAGATATGGAGGAGCTAAAGGAGTGTTGACAATTGATTATAA
AAGTGATAAAAAAAATACAATATAGTTGAGAGAAAGTATGATAAAATATAAAATTACAGA
TGAATAAGACGAATTAATAATTGATATTCTAGATAGAAACAAATATAGATTTGGATATTT
GAATAGGTAAGTCATTATCTTACTTAGATCACTAGGTATTGAAAACGAATAATTCATCTA
AGCTCAGCAAGAATACATAGAAGAGTTAAATAAGTCAGCATTGAAAGATGCAAGTATATT
TACACTTTTTAACACAGATTATTCAAAGGATGGAACAAGATTATTTCCAATCACCCATCT
TCTAAGAAGTATGTAGACAAGTAGGATTGATATGGCCTAAGATATATTCTGTAAAGGAGT
TATTCATACCCTCAAAAAAAGAGGTTTTTATATTCTGAGAAAGAAGAGTAACATTTTTCT
TAAAAAGAGTGCAAGATTGATGGGTGTTATAGATGAATATAATGTGTTAGAACAAGATGA
AGTTTTTGCATATATAAGTTAGGATTCAGAAAAATCTTTCTTTTAAGTTGAGGGAGATGT
AATTGTAGTTAAAAACCCATGTTTACATCCTGGTGATATTAGAAAATTAAAAGCTGTTAC
TTTGGAAACTATTTCGTAAAGATTAGGAGAAGGAAAAATAAATCCTTTTAAAAAATTAAG
GAACATCCTAATATTCCCTTAAAAAGGTAAAGAGCCTATCACTTCATAAATAGCAGGTTC
CGATTTGGATGGTGATTGCTATTTTATTTGCTGGGATTAAAGATTTATTCCCTTGGAATA
GAAAGAACCTTTTAACTATGATGAAGATGATAATAGTTAAAAAAAATAAATCTCATAAGA
ACAGTATGATATAGAAATCTCTAAAAAGCCTAATACTTAGTTTAAAACTAAAAATCCTGT
ACGAGAAGTGATTGATGATAATGAATAAATGCTAGACTTTTTTATAGATTTTATAAATAA
TGAAAATCTTGGTAAAATAGATAATTCTCATTTAGTCTTTGCAGATAAAAGTCCATTATA
CGCTGAAGACCCTAAATGTTTAAAGTTGGCATCACTACATGCATAAGCTGTAGATTATGC
AAAAACAGGTATTTCTCCTTAATTACCAAAAAACCTTGCAATAAAAGAGTACCCTGATTT
TATGGAAAAAGATGACAAACCATCTTATTAATCATAAAGCATTTTAGGTATTTTATATAG
ACAAGTAAAAGACCAGTATGAAAAACACTGTAAAAACGATATAGAAGGAGATAGATCTTA
AACTTTTATTGATAATAATTTAATATTCAATCCGCCTACATTTGTTAACGAAATTGTAGA
TGAGTAAGAAAATAAAACTTATCTTGAATAGGAAATTGATTTCGCTATTGAAGCAAATAA
TCAATGGAATATAGAAATAAATAATCTCAAGCAATTCTTTGGTGCTCAAAATGAGTTTGA
AATTTACACAGGTAATTTTTCCAAACTCATAAAAGTAGAGAGGAAAAAAAAGATGAGTGC
AGAAATAGTATAGCAAAGAATCTTGTCTACATTATTAGCAATAAAAAATAGGTTTGAGCT
TTTGTTCTTTAAAAATTTAGATGAAGATAGAGATAGATTTTATGATGAAACAAACAGAAA
AAAATATTATAGTAAAAAATGTAAAATAAGAGCTACTTTATGGTATATCATATGCTACTT
AAGAAAACCTGAACATATTGTTTAATATGCAGTAAACTTTGAGAAAGATTCTTTACTAGG
ACAGACTTTCACTTTTTTGAGTCAATAAAAATATTTTGGAGAAACAATCATAAGAGGAAA
TAGAAATCCAAAAACTATTCAATTCTTCAATGAAAATAATGATTATGTTGGCTTATCATG
GTTTATAATTAGTAATGTCCTGTTAGAAATAAAGAAAGATAATGATAACTATAAAATGAA
TGAATAAAAATAATAAAAAAGTGAATAATAAATAAAGGAATTTGAAGATTATAAAAAAAA
ATTCTATTAAAATGATTTAAATGGATACCATTCTGACGAATATTTATGATTTTCAGCTAG
TTAGCCAGATATTTATTTGCTAGATATAGCATTAAAAGAAATTTAAAATATGTTATTTAT
ATATTTTTAATAATACTTTGCAAAAATTTATGCTAACAAGTATTTAATACAATAATGATA
CATATATAAAATAAATAA

>TTHERM_00853150(protein)
MIEQSIEEEKIICCIGYINGKITSKNFTTSLCFASIQKYLQEQQHRFKSIKCVSIYTPKK
EQQQIGLDTIDDVKEKIKEIQKSIDHNYLNIPQHLKEERYRVNYKSLNFQEGQQSLDNDD
DSNLLGSEYQFLKNFITKENDDIHLKEEMASFNKIESFCQNVLKNSKNESKINTVNQQKD
QYARINSWIIILSGTEQDLEVLNDSKIDVKTKHEKYYFFLKTKHNDNEKKNYLKICTDEL
SNYAKEQMNFVNIKLLQVSLGIFELYGGALYYNCQFQFEENIDYVNTISIYMQNDESESK
QKQQSVSIVLAYNDYQLKIEFPYLYCNEIRFDQRGKNMYYFQLVVPPKYFVRENSANIQE
NLDQKKNTWIRVDNFFAHDISNELKRIEFVNNTVICIEIENETVNTFQTILKNSFSNVFK
DTDPIPISNVSQHFPQEPENDLDQQWERLLNRYSFNLSYSILTLISHNKLSVVKLREFLD
YIDLQIRDGKSEYILEKGIQYYYNQKKEINRLNFTAFQILFQDVQRQENYFKIQEIRAKR
DVKVIATRRVSITPSGLIYNLKELELSNRVLRIFQDSTDSFIRGTFQDDSLENAKHMKYV
SRYFRQKLKSFKVLNWEFINFGWSASQLRNSSSWFFHETEVLKKQDVITRIGDFSGINPP
AKKSARIGQCFSSSTPYILKERPNIEFIDDITTPSAVQEVDANGNICYFRQKKKVFTDGI
GTISQDLIRKISDHLNAFFMCAIQVRYGGAKGVLTIDYKSDKKNTIQLRESMIKYKITDE
QDELIIDILDRNKYRFGYLNRQVIILLRSLGIENEQFIQAQQEYIEELNKSALKDASIFT
LFNTDYSKDGTRLFPITHLLRSMQTSRIDMAQDIFCKGVIHTLKKRGFYILRKKSNIFLK
KSARLMGVIDEYNVLEQDEVFAYISQDSEKSFFQVEGDVIVVKNPCLHPGDIRKLKAVTL
ETISQRLGEGKINPFKKLRNILIFPQKGKEPITSQIAGSDLDGDCYFICWDQRFIPLEQK
EPFNYDEDDNSQKKQISQEQYDIEISKKPNTQFKTKNPVREVIDDNEQMLDFFIDFINNE
NLGKIDNSHLVFADKSPLYAEDPKCLKLASLHAQAVDYAKTGISPQLPKNLAIKEYPDFM
EKDDKPSYQSQSILGILYRQVKDQYEKHCKNDIEGDRSQTFIDNNLIFNPPTFVNEIVDE
QENKTYLEQEIDFAIEANNQWNIEINNLKQFFGAQNEFEIYTGNFSKLIKVERKKKMSAE
IVQQRILSTLLAIKNRFELLFFKNLDEDRDRFYDETNRKKYYSKKCKIRATLWYIICYLR
KPEHIVQYAVNFEKDSLLGQTFTFLSQQKYFGETIIRGNRNPKTIQFFNENNDYVGLSWF
IISNVLLEIKKDNDNYKMNEQKQQKSEQQIKEFEDYKKKFYQNDLNGYHSDEYL