Identifiers and Description

Gene Model Identifier

TTHERM_00865050

Standard Name

ORC1 (origin recognition complex subunit 1)

Aliases

PreTt29135 | 154.m00093 | 3751.m00439

Description

ORC1 origin recognition complex subunit 1 putative; Largest subunit of the origin recognition complex

Genome Browser (Macronucleus)

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Genome Browser (Micronucleus)

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Gene Ontology Annotations

No Data fetched for Gene Ontology Annotations

Domains

No Data fetched for Domains

Gene Expression Profile

Tetrahymena Stock Center

No Data fetched for Tetrahymena Stock Center

Homologs

Source Identifier Score
Tetrahymena borealis EI9_19673.1 9.997093137033517e-262
Description: hypothetical protein (2343 aa)
Oxytricha Contig20978.0.g107 1.9997677415930956e-65
Description: ATPase family associated with various cellular activities (AAA)
DictyBase DDB_G0283073 9.995951036711152e-55
Description: orcA on chromosome: 4 position 182130 to 184025
WormBase WBGene00012650 3.998097515166115e-39
Description: locus:orc-1 status:Partially_confirmed UniProt:Q9XX17 protein_id:CAA21023.1
SGD YML065W 1.0004783691736523e-35
Description: ORC1 Largest subunit of the origin recognition complex, which directs DNA replication by binding to replication origins and is also involved in transcriptional silencing; exhibits ATPase activity
Stentor Coeruleus SteCoe_36947 9.602680054508676e-24
Description: None

General Information

No. Gene Name(s) Paragraph Text
61 ORC1 Gene Information: TTHERM_00865050 is a homolog of annotated and functionally characterized Orc1 in other experimental organisms (24% amino acid sequence identity, 49% similarity to human Orc1). Orc1 is a component of the heterohexameric origin recognition complex (ORC) found to be involved in initiation of DNA replication. TtOrc1 has canonical Walker A and B motifs (involved in ATP binding and hydrolysis). TtORC is unusual in that it contains an integral RNA subunit (26T RNA) that binds to its cognate DNA target in the ribosomal DNA (rDNA) replication origin.
62 ORC1 Nucleic Acid Interactions (with the ORC complex): 26T RNA (5’-AUGUCUAAGUGUGAUGAUAAACGAAAAAAAAUAAAAAUUAA-3’). Type I element T-rich strand: essential cis-acting replication determinant in ribosomal DNA origin of replication. (Location 5’ non-transcribed spacer) (C3 rDNA type IB element T-rich strand, T51: 5’-CTCAAAAGTTGCAAAAGTTCGGAAGGTTTACTATTTTTGTTTTTTTTTT-3’). – requires 26T RNA and ATP dsDNA – non rDNA chromosomes
63 ORC1 Protein Interaction Partners: TtOrc2 (Co-migration on a native gel, detection by WB).
64 ORC1 Biochemical Activities (of ORC complex): ATP binding, likely ATP hydrolysis DNA binding - typeI element T-rich strand DNA RNA binding – 26T RNA
65 ORC1 Regulation & Expression: mRNA and protein: Cell cycle regulated Maximal protein expression at G1/S border, degraded in S phase

Associated Literature

  1. Ref:19153611: Donti TR, Datta S, Sandoval PY, Kapler GM (2009) Differential targeting of Tetrahymena ORC to ribosomal DNA and non-rDNA replication origins. The EMBO journal 28(3):223-33
  2. Ref:18007594: Mohammad MM, Donti TR, Sebastian Yakisich J, Smith AG, Kapler GM (2007) Tetrahymena ORC contains a ribosomal RNA fragment that participates in rDNA origin recognition. The EMBO journal 26(24):5048-60
  3. Ref:12832485: Mohammad M, York RD, Hommel J, Kapler GM (2003) Characterization of a novel origin recognition complex-like complex: implications for DNA recognition, cell cycle control, and locus-specific gene amplification. Molecular and cellular biology 23(14):5005-17

Sequences 

>TTHERM_00865050(coding)
ATGAACACAAGAAATACATAAAGGTCAATAGATCCAAAAAAAGGAAAGTAGCAATAAAAA
TCAGTTAGCAAAAGTAGAAGCAAAAGCAATAATATAACTTAAAGCAATGAAAAGATAAAA
AGGAGTTAAAGATTAAAGTCAAAAGATGAGATGATAGATTCGGTTTAAGAACCCAATATT
TTCGTCTAGAAAGATAAAAATAAACAATAATAATCTAAAAGTAAATCTAAGAATTCAACA
AAAGAATCAGAAAAAAAGCCTTAAAAAAGAGCATTTAAAATTGAGATACCTAAATAAAAT
GAATAAAAATTAAAAAAAACTTAAGAATCAAATAAAACAATAATAAATTTGACTAAATAG
TAGAAATAACAATTAAAATCATAATAGCTAACAAAAGCTGTAATTGAGTAATAAATACAC
ACTGAATCAAAGAAAAGGGAATACAATAAGCAAAAGAATAAAAATGATAATTTAGAAAAA
ACTAAAAATAATCATAATATTGTTACTATTGATTCCAGCAATGATGAAAATGAAAGTGAA
TATGAAATTTCAGATTGTCATATTTCAGTTTCTGTATATAATAATGATAATGAAATAGAT
ATAGATAAAAATGAATAGTCTGTAGATGTAAAGTAAAATAAAAATAAAAATATGATAGAT
AGTAGAGGAAGAAGTAAAAATAGCCTTAAAATTAAAAAAACATATTCTTCTAGCTAGTAA
AAGAGTTAAGAAAAATAGTAAGTTAAAGAATCAACTGCCAGCAAAAGGATTACTCGTAGC
CAATAAAATTTTGAACTTACAGAGTTATAAGATACAAAGGCAAAAAGTGCTTCAAAAAAC
AAAACAATAAATTCGGAAAAGCAAAATTAAAAAATGAATTCTAAAGAAAAAATTGATACA
AGCAAAGACAAGAATCCATCATTAATTGAAAGTGAAAAACCTAAAAGAGGAAGAAAGCCT
AAATAAGTAGAGATATTAAATGATAAAATTTAAGAAAAAGAGACAGATTAGTAAAAAACA
CCTAAAAAGAGGGGGAGAAAAAGTAAATAAGAAGCATTGTTAGATAGAAGAAACATTAAG
GAGATATAATAATAAAAAGAGTACGAAGAGAAATAATAAAAGAAATCTTCATCTAAAGAA
TCTTAAAAATTAACTCAAGCCAGCGAACATTTTAACTTTTAAGCGCTAATTTATTAATAA
AAACCCGCCAAAAAAGAGTTACGTATTGAAGACAAGAATTATTAAAAAATAAATGTTAAA
CCAAAACATAATATTTATGATTAAGCTATAGAAATGCTATAAGAATATTCATTGCCAGAA
GAAATTCCTTGTAGAGAAGATGAAAAGAAATAAATTTTAGAATTTATAAATGAAGGTTTA
GGCAATAATGGTAGCTCTAATTGCTTGTATATTTCAGGTGTTCCTGGTATAGGAAAGACA
GCAAGTTTTTTGGAAGTGATTAAAAAATTATAAAATGAAAAAAAAGATGAATTTACTTTC
ATTCACATTAATGCTATGAATTTAAGTAACCCAGAAAATTTATACTATATACTCGTTAAA
ACAATAACTGGGAAAAATTGCACATCAAAATAAAAAGCCTGTTAAATTTTAAATGAGCTT
TTCACTCGTGGAAAACTTTCAAAAACATACTAAAGCTATGGAGAAAATATTGATAATAAA
AATAAGGTGATTCTCTTAGATGAATTAGATTATTTAGTCACATAAGATTAGGATTTACTT
TATAACTTAATGGAATGGCCTCATCATAAATACTCAAAATTAACTATTATTGGGATAGCT
AATACTATGAATTTACCAGAAATTTTGATGAACAAAATTAAAAGTAGAATGGGAAGTAGA
AGACTTGTTTTTAATTAGTATAATCATAAATAAATACAGGAAATTATTGCTACTAGACTT
AAAAATTAGGAAAAAGTTAGGGAAGTGTTTGAGTAAAATGCAATAGAATATACATGCAGA
AAAATTGCTATTTCATCTTCAGATATAAGAAAAACATTGAAAGTGTTAAGAAAAGCTGTT
GAAATATGTCAGTTAGAAAACTTTTAAAATTAAAATGTTACAAAAGTAACTATTCCTATG
ATTTAAAAATCGTATAGCTAGTTATACTCTTCTCCTATTCTATATTCAATTTAAAAACTT
TAATTTCATCATAAGTTGATGATTCTTTCTATAGCTTTAGAAAATAAGCACAGAGGAATA
CCAGTAGCTTACTTGTCAGAGAGCTATAATAGATATAGAATAATGTTGAGCAATCTAGGT
AAAAACGAAACTATTAATTATAATCAAATGAAAATGGTCCTGAACTAACTTTCTGTTTTA
AATTTAATACAATTGAAGGAAAAAGAAATAGTTAAAGATACACAATTAATTGGTTTAGAT
CCTAATAAGAAACACTTTTGTCATGTTGTTGACATTTAAATTAATTCTAATTTTAGTGTT
GATGATGTCACTAACTCTCTAAAAGAAGATGAAATTTATTTGAAATTCTAACATTAATTC
TGA


>TTHERM_00865050(gene)
ATGAACACAAGAAATACATAAAGGTCAATAGATCCAAAAAAAGGAAAGTAGCAATAAAAA
TCAGTTAGCAAAAGTAGAAGCAAAAGCAATAATATAACTTAAAGCAATGAAAAGATAAAA
AGGAGTTAAAGATTAAAGTCAAAAGATGAGATGATAGATTCGGTTTAAGAACCCAATATT
TTCGTCTAGAAAGATAAAAATAAACAATAATAATCTAAAAGTAAATCTAAGAATTCAACA
AAAGAATCAGAAAAAAAGCCTTAAAAAAGAGCATTTAAAATTGAGATACCTAAATAAAAT
GAATAAAAATTAAAAAAAACTTAAGAATCAAATAAAACAATAATAAATTTGACTAAATAG
TAGAAATAACAATTAAAATCATAATAGCTAACAAAAGCTGTAATTGAGTAATAAATACAC
ACTGAATCAAAGAAAAGGGAATACAATAAGCAAAAGAATAAAAATGATAATTTAGAAAAA
ACTAAAAATAATCATAATATTGTTACTATTGATTCCAGCAATGATGAAAATGAAAGTGAA
TATGAAATTTCAGATTGTCATATTTCAGTTTCTGTATATAATAATGATAATGAAATAGAT
ATAGATAAAAATGAATAGTCTGTAGATGTAAAGTAAAATAAAAATAAAAATATGATAGAT
AGTAGAGGAAGAAGTAAAAATAGCCTTAAAATTAAAAAAACATATTCTTCTAGCTAGTAA
AAGAGTTAAGAAAAATAGTAAGTTAAAGAATCAACTGCCAGCAAAAGGATTACTCGTAGC
CAATAAAATTTTGAACTTACAGAGTTATAAGATACAAAGGCAAAAAGTGCTTCAAAAAAC
AAAACAATAAATTCGGAAAAGCAAAATTAAAAAATGAATTCTAAAGAAAAAATTGATACA
AGCAAAGACAAGAATCCATCATTAATTGAAAGTGAAAAACCTAAAAGAGGAAGAAAGCCT
AAATAAGTAGAGATATTAAATGATAAAATTTAAGAAAAAGAGACAGATTAGTAAAAAACA
CCTAAAAAGAGGGGGAGAAAAAGTAAATAAGAAGCATTGTTAGATAGAAGAAACATTAAG
GAGATATAATAATAAAAAGAGTACGAAGAGAAATAATAAAAGAAATCTTCATCTAAAGAA
TCTTAAAAATTAACTCAAGCCAGCGAACATTTTAACTTTTAAGCGCTAATTTATTAATAA
AAACCCGCCAAAAAAGAGTTACGTATTGAAGACAAGAATTATTAAAAAATAAATGTTAAA
CCAAAACATAATATTTATGATTAAGCTATAGAAATGCTATAAGAATATTCATTGCCAGAA
GAAATTCCTTGTAGAGAAGATGAAAAGAAATAAATTTTAGAATTTATAAATGAAGGTTTA
GGCAATAATGGTAGCTCTAATTGCTTGTGTAATCAATTAATTTTAAATTCTTTTTAAATT
TTCATAAAATTTAAAAAAATTTATTAATCATAAATTAAAAATTTAGATATTTCAGGTGTT
CCTGGTATAGGAAAGACAGCAAGTTTTTTGGAAGTGATTAAAAAATTATAAAATGAAAAA
AAAGATGAATTTACTTTCATTCACATTAATGCTATGAATTTAAGTAACCCAGAAAATTTA
TACTATATACTCGTTAAAACAATAACTGGGAAAAATTGCACATCAAAATAAAAAGCCTGT
TAAATTTTAAGTAAGCAATTAATTTATTAATACTTAAAAAATTAATTTATTGTTTTAATT
TTATTAGATGAGCTTTTCACTCGTGGAAAACTTTCAAAAACATACTAAAGCTATGGAGAA
AATATTGATAATAAAAATAAGTAATTTTTGACTAAAAAATAATAAAACTATATTTAAAAT
ACTATTTTAATTAAATTCAGGGTGATTCTCTTAGATGAATTAGATTATTTAGTCACATAA
GATTAGGATTTACTTTATAACTTAATGGAATGGCCTCATCATAAATACTCAAAATTAACT
ATTATTGGGATAGCTAATACTATGAATTTACCAGAAATTTTGATGAACAAAATTAAAAGT
AGAATGGGAAGTAGAAGACTTGTTTTTAATTAGTATAATCATAAATAAATACAGGAAATT
ATTGCTACTAGACTTAAAAATTAGGAAAAAGTTAGGGAAGTGTTTGAGTAAAATGCAATA
GAATATACATGCAGAAAAATTGCTATTTCATCTTCAGATATAAGAAAAACATTGAAAGTG
TTAAGAAAAGCTGTTGAAATATGTCAGTTAGAAAACTTTTAAAATTAAAATGTTACAAAA
GTAACTATTCCTATGATTTAAAAATCGTATAGCTAGTTATACTCTTCTCCTATTCTATAT
TCAATTTAAAAACTTTAATTTCATCATAAGTTGATGATTCTTTCTATAGCTTTAGAAAAT
AAGCACAGAGGAATACCAGTAGCTTACTTGTCAGAGGTAAATATTTAAATTCTAAATTTA
TATAATTTTTATTTATATATTTATGATATAGAGCTATAATAGATATAGAATAATGTTGAG
CAATCTAGGTAAAAACGAAACTATTAATTATAATCAAATGAAAATGGTCCTGAACTAACT
TTCTGTTTTAAATTTAATACAATTGAAGGAAAAAGAAATAGTTAAAGATACACAATTAAT
TGGTTTAGATCCTAATAAGAAACACTTTTGTCATGTTGTTGACATTTAAATTAATTCTAA
TTTTAGTGTTGATGATGTCACTAACTCTCTAAAAGAAGATGAAATTTATTTGAAATTCTA
ACATTAATTCTGA


>TTHERM_00865050(protein)
MNTRNTQRSIDPKKGKQQQKSVSKSRSKSNNITQSNEKIKRSQRLKSKDEMIDSVQEPNI
FVQKDKNKQQQSKSKSKNSTKESEKKPQKRAFKIEIPKQNEQKLKKTQESNKTIINLTKQ
QKQQLKSQQLTKAVIEQQIHTESKKREYNKQKNKNDNLEKTKNNHNIVTIDSSNDENESE
YEISDCHISVSVYNNDNEIDIDKNEQSVDVKQNKNKNMIDSRGRSKNSLKIKKTYSSSQQ
KSQEKQQVKESTASKRITRSQQNFELTELQDTKAKSASKNKTINSEKQNQKMNSKEKIDT
SKDKNPSLIESEKPKRGRKPKQVEILNDKIQEKETDQQKTPKKRGRKSKQEALLDRRNIK
EIQQQKEYEEKQQKKSSSKESQKLTQASEHFNFQALIYQQKPAKKELRIEDKNYQKINVK
PKHNIYDQAIEMLQEYSLPEEIPCREDEKKQILEFINEGLGNNGSSNCLYISGVPGIGKT
ASFLEVIKKLQNEKKDEFTFIHINAMNLSNPENLYYILVKTITGKNCTSKQKACQILNEL
FTRGKLSKTYQSYGENIDNKNKVILLDELDYLVTQDQDLLYNLMEWPHHKYSKLTIIGIA
NTMNLPEILMNKIKSRMGSRRLVFNQYNHKQIQEIIATRLKNQEKVREVFEQNAIEYTCR
KIAISSSDIRKTLKVLRKAVEICQLENFQNQNVTKVTIPMIQKSYSQLYSSPILYSIQKL
QFHHKLMILSIALENKHRGIPVAYLSESYNRYRIMLSNLGKNETINYNQMKMVLNQLSVL
NLIQLKEKEIVKDTQLIGLDPNKKHFCHVVDIQINSNFSVDDVTNSLKEDEIYLKFQHQF