Identifiers and Description

Gene Model Identifier

TTHERM_00865050

Standard Name

ORC1 (origin recognition complex subunit 1)

Aliases

PreTt29135 | 154.m00093 | 3751.m00439

Description

ORC1 origin recognition complex subunit 1 putative; Largest subunit of the origin recognition complex; Origin Recognition Complex 1/Cell Division Control Protein 6

Genome Browser (Macronucleus)

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Genome Browser (Micronucleus)

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External Links

Gene Ontology Annotations

No Data fetched for Gene Ontology Annotations

Domains

  • ( PF00004 ) ATPase family associated with various cellular activities (AAA)
  • ( PF17872 ) AAA lid domain

Gene Expression Profile

Vegetative Cell Cycle (Zhang et al., 2023)

GeneMania

Tetrahymena Stock Center

No Data fetched for Tetrahymena Stock Center

Homologs

No Data fetched for Homologs

General Information

No. Gene Name(s) Paragraph Text
2267 ORC1 Gene Information: TTHERM_00865050 is a homolog of annotated and functionally characterized Orc1 in other experimental organisms (24% amino acid sequence identity, 49% similarity to human Orc1). Orc1 is a component of the heterohexameric origin recognition complex (ORC) found to be involved in initiation of DNA replication. TtOrc1 has canonical Walker A and B motifs (involved in ATP binding and hydrolysis). TtORC is unusual in that it contains an integral RNA subunit (26T RNA) that binds to its cognate DNA target in the ribosomal DNA (rDNA) replication origin.
2268 ORC1 Nucleic Acid Interactions (with the ORC complex): 26T RNA (5’-AUGUCUAAGUGUGAUGAUAAACGAAAAAAAAUAAAAAUUAA-3’). Type I element T-rich strand: essential cis-acting replication determinant in ribosomal DNA origin of replication. (Location 5’ non-transcribed spacer) (C3 rDNA type IB element T-rich strand, T51: 5’-CTCAAAAGTTGCAAAAGTTCGGAAGGTTTACTATTTTTGTTTTTTTTTT-3’). – requires 26T RNA and ATP dsDNA – non rDNA chromosomes
2269 ORC1 Protein Interaction Partners: TtOrc2 (Co-migration on a native gel, detection by WB).
2270 ORC1 Biochemical Activities (of ORC complex): ATP binding, likely ATP hydrolysis DNA binding - typeI element T-rich strand DNA RNA binding – 26T RNA
2271 ORC1 Regulation & Expression: mRNA and protein: Cell cycle regulated Maximal protein expression at G1/S border, degraded in S phase

Associated Literature

  1. Ref:19153611: Donti TR, Datta S, Sandoval PY, Kapler GM (2009) Differential targeting of Tetrahymena ORC to ribosomal DNA and non-rDNA replication origins. The EMBO journal 28(3):223-33
  2. Ref:18007594: Mohammad MM, Donti TR, Sebastian Yakisich J, Smith AG, Kapler GM (2007) Tetrahymena ORC contains a ribosomal RNA fragment that participates in rDNA origin recognition. The EMBO journal 26(24):5048-60
  3. Ref:12832485: Mohammad M, York RD, Hommel J, Kapler GM (2003) Characterization of a novel origin recognition complex-like complex: implications for DNA recognition, cell cycle control, and locus-specific gene amplification. Molecular and cellular biology 23(14):5005-17

Sequences 

>TTHERM_00865050(coding)
ATGAACACAAGAAATACATAAAGGTCAATAGATCCAAAAAAAGGAAAGTAGCAATAAAAA
TCAGTTAGCAAAAGTAGAAGCAAAAGCAATAATATAACTTAAAGCAATGAAAAGATAAAA
AGGAGTTAAAGATTAAAGTCAAAAGATGAGATGATAGATTCGGTTTAAGAACCCAATATT
TTCGTCTAGAAAGATAAAAATAAACAATAATAATCTAAAAGTAAATCTAAGAATTCAACA
AAAGAATCAGAAAAAAAGCCTTAAAAAAGAGCATTTAAAATTGAGATACCTAAATAAAAT
GAATAAAAATTAAAAAAAACTTAAGAATCAAATAAAACAATAATAAATTTGACTAAATAG
TAGAAATAACAATTAAAATCATAATAGCTAACAAAAGCTGTAATTGAGTAATAAATACAC
ACTGAATCAAAGAAAAGGGAATACAATAAGCAAAAGAATAAAAATGATAATTTAGAAAAA
ACTAAAAATAATCATAATATTGTTACTATTGATTCCAGCAATGATGAAAATGAAAGTGAA
TATGAAATTTCAGATTGTCATATTTCAGTTTCTGTATATAATAATGATAATGAAATAGAT
ATAGATAAAAATGAATAGTCTGTAGATGTAAAGTAAAATAAAAATAAAAATATGATAGAT
AGTAGAGGAAGAAGTAAAAATAGCCTTAAAATTAAAAAAACATATTCTTCTAGCTAGTAA
AAGAGTTAAGAAAAATAGTAAGTTAAAGAATCAACTGCCAGCAAAAGGATTACTCGTAGC
CAATAAAATTTTGAACTTACAGAGTTATAAGATACAAAGGCAAAAAGTGCTTCAAAAAAC
AAAACAATAAATTCGGAAAAGCAAAATTAAAAAATGAATTCTAAAGAAAAAATTGATACA
AGCAAAGACAAGAATCCATCATTAATTGAAAGTGAAAAACCTAAAAGAGGAAGAAAGCCT
AAATAAGTAGAGATATTAAATGATAAAATTTAAGAAAAAGAGACAGATTAGTAAAAAACA
CCTAAAAAGAGGGGGAGAAAAAGTAAATAAGAAGCATTGTTAGATAGAAGAAACATTAAG
GAGATATAATAATAAAAAGAGTACGAAGAGAAATAATAAAAGAAATCTTCATCTAAAGAA
TCTTAAAAATTAACTCAAGCCAGCGAACATTTTAACTTTTAAGCGCTAATTTATTAATAA
AAACCCGCCAAAAAAGAGTTACGTATTGAAGACAAGAATTATTAAAAAATAAATGTTAAA
CCAAAACATAATATTTATGATTAAGCTATAGAAATGCTATAAGAATATTCATTGCCAGAA
GAAATTCCTTGTAGAGAAGATGAAAAGAAATAAATTTTAGAATTTATAAATGAAGGTTTA
GGCAATAATGGTAGCTCTAATTGCTTGTATATTTCAGGTGTTCCTGGTATAGGAAAGACA
GCAAGTTTTTTGGAAGTGATTAAAAAATTATAAAATGAAAAAAAAGATGAATTTACTTTC
ATTCACATTAATGCTATGAATTTAAGTAACCCAGAAAATTTATACTATATACTCGTTAAA
ACAATAACTGGGAAAAATTGCACATCAAAATAAAAAGCCTGTTAAATTTTAAATGAGCTT
TTCACTCGTGGAAAACTTTCAAAAACATACTAAAGCTATGGAGAAAATATTGATAATAAA
AATAAGGTGATTCTCTTAGATGAATTAGATTATTTAGTCACATAAGATTAGGATTTACTT
TATAACTTAATGGAATGGCCTCATCATAAATACTCAAAATTAACTATTATTGGGATAGCT
AATACTATGAATTTACCAGAAATTTTGATGAACAAAATTAAAAGTAGAATGGGAAGTAGA
AGACTTGTTTTTAATTAGTATAATCATAAATAAATACAGGAAATTATTGCTACTAGACTT
AAAAATTAGGAAAAAGTTAGGGAAGTGTTTGAGTAAAATGCAATAGAATATACATGCAGA
AAAATTGCTATTTCATCTTCAGATATAAGAAAAACATTGAAAGTGTTAAGAAAAGCTGTT
GAAATATGTCAGTTAGAAAACTTTTAAAATTAAAATGTTACAAAAGTAACTATTCCTATG
ATTTAAAAATCGTATAGCTAGTTATACTCTTCTCCTATTCTATATTCAATTTAAAAACTT
TAATTTCATCATAAGTTGATGATTCTTTCTATAGCTTTAGAAAATAAGCACAGAGGAATA
CCAGTAGCTTACTTGTCAGAGAGCTATAATAGATATAGAATAATGTTGAGCAATCTAGGT
AAAAACGAAACTATTAATTATAATCAAATGAAAATGGTCCTGAACTAACTTTCTGTTTTA
AATTTAATACAATTGAAGGAAAAAGAAATAGTTAAAGATACACAATTAATTGGTTTAGAT
CCTAATAAGAAACACTTTTGTCATGTTGTTGACATTTAAATTAATTCTAATTTTAGTGTT
GATGATGTCACTAACTCTCTAAAAGAAGATGAAATTTATTTGAAATTCTAACATTAATTC
TGA



>TTHERM_00865050(gene)
TATCTTTAGATTAAATAAATCAAAAATAAAAAATTATCTTCAAAATGAACACAAGAAATA
CATAAAGGTCAATAGATCCAAAAAAAGGAAAGTAGCAATAAAAATCAGTTAGCAAAAGTA
GAAGCAAAAGCAATAATATAACTTAAAGCAATGAAAAGATAAAAAGGAGTTAAAGATTAA
AGTCAAAAGATGAGATGATAGATTCGGTTTAAGAACCCAATATTTTCGTCTAGAAAGATA
AAAATAAACAATAATAATCTAAAAGTAAATCTAAGAATTCAACAAAAGAATCAGAAAAAA
AGCCTTAAAAAAGAGCATTTAAAATTGAGATACCTAAATAAAATGAATAAAAATTAAAAA
AAACTTAAGAATCAAATAAAACAATAATAAATTTGACTAAATAGTAGAAATAACAATTAA
AATCATAATAGCTAACAAAAGCTGTAATTGAGTAATAAATACACACTGAATCAAAGAAAA
GGGAATACAATAAGCAAAAGAATAAAAATGATAATTTAGAAAAAACTAAAAATAATCATA
ATATTGTTACTATTGATTCCAGCAATGATGAAAATGAAAGTGAATATGAAATTTCAGATT
GTCATATTTCAGTTTCTGTATATAATAATGATAATGAAATAGATATAGATAAAAATGAAT
AGTCTGTAGATGTAAAGTAAAATAAAAATAAAAATATGATAGATAGTAGAGGAAGAAGTA
AAAATAGCCTTAAAATTAAAAAAACATATTCTTCTAGCTAGTAAAAGAGTTAAGAAAAAT
AGTAAGTTAAAGAATCAACTGCCAGCAAAAGGATTACTCGTAGCCAATAAAATTTTGAAC
TTACAGAGTTATAAGATACAAAGGCAAAAAGTGCTTCAAAAAACAAAACAATAAATTCGG
AAAAGCAAAATTAAAAAATGAATTCTAAAGAAAAAATTGATACAAGCAAAGACAAGAATC
CATCATTAATTGAAAGTGAAAAACCTAAAAGAGGAAGAAAGCCTAAATAAGTAGAGATAT
TAAATGATAAAATTTAAGAAAAAGAGACAGATTAGTAAAAAACACCTAAAAAGAGGGGGA
GAAAAAGTAAATAAGAAGCATTGTTAGATAGAAGAAACATTAAGGAGATATAATAATAAA
AAGAGTACGAAGAGAAATAATAAAAGAAATCTTCATCTAAAGAATCTTAAAAATTAACTC
AAGCCAGCGAACATTTTAACTTTTAAGCGCTAATTTATTAATAAAAACCCGCCAAAAAAG
AGTTACGTATTGAAGACAAGAATTATTAAAAAATAAATGTTAAACCAAAACATAATATTT
ATGATTAAGCTATAGAAATGCTATAAGAATATTCATTGCCAGAAGAAATTCCTTGTAGAG
AAGATGAAAAGAAATAAATTTTAGAATTTATAAATGAAGGTTTAGGCAATAATGGTAGCT
CTAATTGCTTGTATATTTCAGGTGTTCCTGGTATAGGAAAGACAGCAAGTTTTTTGGAAG
TGATTAAAAAATTATAAAATGAAAAAAAAGATGAATTTACTTTCATTCACATTAATGCTA
TGAATTTAAGTAACCCAGAAAATTTATACTATATACTCGTTAAAACAATAACTGGGAAAA
ATTGCACATCAAAATAAAAAGCCTGTTAAATTTTAAATGAGCTTTTCACTCGTGGAAAAC
TTTCAAAAACATACTAAAGCTATGGAGAAAATATTGATAATAAAAATAAGGTGATTCTCT
TAGATGAATTAGATTATTTAGTCACATAAGATTAGGATTTACTTTATAACTTAATGGAAT
GGCCTCATCATAAATACTCAAAATTAACTATTATTGGGATAGCTAATACTATGAATTTAC
CAGAAATTTTGATGAACAAAATTAAAAGTAGAATGGGAAGTAGAAGACTTGTTTTTAATT
AGTATAATCATAAATAAATACAGGAAATTATTGCTACTAGACTTAAAAATTAGGAAAAAG
TTAGGGAAGTGTTTGAGTAAAATGCAATAGAATATACATGCAGAAAAATTGCTATTTCAT
CTTCAGATATAAGAAAAACATTGAAAGTGTTAAGAAAAGCTGTTGAAATATGTCAGTTAG
AAAACTTTTAAAATTAAAATGTTACAAAAGTAACTATTCCTATGATTTAAAAATCGTATA
GCTAGTTATACTCTTCTCCTATTCTATATTCAATTTAAAAACTTTAATTTCATCATAAGT
TGATGATTCTTTCTATAGCTTTAGAAAATAAGCACAGAGGAATACCAGTAGCTTACTTGT
CAGAGAGCTATAATAGATATAGAATAATGTTGAGCAATCTAGGTAAAAACGAAACTATTA
ATTATAATCAAATGAAAATGGTCCTGAACTAACTTTCTGTTTTAAATTTAATACAATTGA
AGGAAAAAGAAATAGTTAAAGATACACAATTAATTGGTTTAGATCCTAATAAGAAACACT
TTTGTCATGTTGTTGACATTTAAATTAATTCTAATTTTAGTGTTGATGATGTCACTAACT
CTCTAAAAGAAGATGAAATTTATTTGAAATTCTAACATTAATTCTGAACAGTCGGAATCT
GTTTTAAAATAACCTCTCTTTTTTTTTAATTAATTGATTAAATTACTGCTAATCTAGAAC
ATTATATCTTATAATTAATAATACTACTTTGTACATATCTAAATTTTATGCTTTCGTATT
TTATACCAGCTAGATATATAAATAT



>TTHERM_00865050(protein)
MNTRNTQRSIDPKKGKQQQKSVSKSRSKSNNITQSNEKIKRSQRLKSKDEMIDSVQEPNI
FVQKDKNKQQQSKSKSKNSTKESEKKPQKRAFKIEIPKQNEQKLKKTQESNKTIINLTKQ
QKQQLKSQQLTKAVIEQQIHTESKKREYNKQKNKNDNLEKTKNNHNIVTIDSSNDENESE
YEISDCHISVSVYNNDNEIDIDKNEQSVDVKQNKNKNMIDSRGRSKNSLKIKKTYSSSQQ
KSQEKQQVKESTASKRITRSQQNFELTELQDTKAKSASKNKTINSEKQNQKMNSKEKIDT
SKDKNPSLIESEKPKRGRKPKQVEILNDKIQEKETDQQKTPKKRGRKSKQEALLDRRNIK
EIQQQKEYEEKQQKKSSSKESQKLTQASEHFNFQALIYQQKPAKKELRIEDKNYQKINVK
PKHNIYDQAIEMLQEYSLPEEIPCREDEKKQILEFINEGLGNNGSSNCLYISGVPGIGKT
ASFLEVIKKLQNEKKDEFTFIHINAMNLSNPENLYYILVKTITGKNCTSKQKACQILNEL
FTRGKLSKTYQSYGENIDNKNKVILLDELDYLVTQDQDLLYNLMEWPHHKYSKLTIIGIA
NTMNLPEILMNKIKSRMGSRRLVFNQYNHKQIQEIIATRLKNQEKVREVFEQNAIEYTCR
KIAISSSDIRKTLKVLRKAVEICQLENFQNQNVTKVTIPMIQKSYSQLYSSPILYSIQKL
QFHHKLMILSIALENKHRGIPVAYLSESYNRYRIMLSNLGKNETINYNQMKMVLNQLSVL
NLIQLKEKEIVKDTQLIGLDPNKKHFCHVVDIQINSNFSVDDVTNSLKEDEIYLKFQHQF