Identifiers and Description

Gene Model Identifier

TTHERM_01104960

Standard Name

BLT1 (Beta-Like Tubulin 1)

Aliases

PreTt01888 | 235.m00039

Description

BLT1 tubulin; Highly divergent beta-like tubulin; present in very low abundance; localizes to small subset of microtubules; not incorporated into growing ciliary axonemes; Tubulin

Genome Browser (Macronucleus)

Full Screen View

No Genome Browser Data Present

Genome Browser (Micronucleus)

Full Screen View

No Genome Browser Data Present

External Links

• Uniprot
• Alphafold

Gene Ontology Annotations

No Data fetched for Gene Ontology Annotations

Domains

• ( PF00091 ) Tubulin/FtsZ family, GTPase domain
• ( PF03953 ) Tubulin C-terminal domain

Gene Expression Profile

Vegetative Cell Cycle (Zhang et al., 2023)

GeneMania

No results were calculated for this gene in GeneMania.

Tetrahymena Stock Center

• ( SD02243 ) Macronucleus: BTU1(AA 1-428) with BLT at C terminus (AA 428-473) followed by HA tag, inserted into the MTT1 locus. Neo2 upstream of MTT1 5’ region
• ( SD02244 ) Macronucleus: BTU1(AA 1-428) with BLT at C terminus (AA 428-473) followed by HA tag, inserted into the MTT1 locus. Neo2 upstream of MTT1 5’ region
• ( SD02245 ) Macronucleus: BTU1(AA 1-428) with BLT at C terminus (AA 428-473) followed by HA tag, inserted into the MTT1 locus. Neo2 upstream of MTT1 5’ region
• ( SD02246 ) Micronucleus: Neo3 replaces BLT coding
• ( SD02247 ) Micronucleus: Neo3 replaces BLT coding
• ( SD02248 ) Micronucleus: Neo3 replaces BLT coding
• ( SD02249 ) Micronucleus: Neo3 replaces BLT coding
• ( SD02250 ) Micronucleus: Neo3 replaces BLT coding
• ( SD02251 ) Micronucleus: Neo3 replaces BLT coding
• ( SD02252 ) Micronucleus: Neo3 replaces BLT coding
• ( SD02253 ) Micronucleus: Neo3 replaces BLT coding
• ( SD02254 ) Micronucleus: Neo3 replaces BLT coding
• ( SD02255 ) Micronucleus: Neo3 replaces BLT coding
• ( SD02256 ) Micronucleus: Neo3 replaces BLT coding
• ( SD02257 ) Micronucleus: Neo3 replaces BLT coding
• ( SD02258 ) Micronucleus: Neo3 replaces BLT coding
• ( SD02259 ) Micronucleus: Neo3 replaces BLT coding
• ( SD02260 ) Macronucleus: BLT1(AA 1-428) withBTU1 at C terminus (AA 429-443) followed by HA tag, inserted into the MTT1 locus. Neo2 upstream of MTT1 5’ region
• ( SD02261 ) Macronucleus: BLT1(AA 1-428) withBTU1 at C terminus (AA 429-443) followed by HA tag, inserted into the MTT1 locus. Neo2 upstream of MTT1 5’ region
• ( SD02262 ) Macronucleus: BLT1(AA 1-428) withBTU1 at C terminus (AA 429-443) followed by HA tag, inserted into the MTT1 locus. Neo2 upstream of MTT1 5’ region
• ( SD02562 ) Macronucleus: BLT1with c-terminal HA replaces MTTi coding with neo2 upsteam in Mtt1 5’ flanking region
• ( SD02563 ) Macronucleus: BLT1with c-terminal HA replaces MTTi coding with neo2 upsteam in Mtt1 5’ flanking region
• ( SD02564 ) Macronucleus: BLT1with c-terminal HA replaces MTTi coding with neo2 upsteam in Mtt1 5’ flanking region
• ( SD02565 ) Macronucleus: BLT1with c-terminal HA replaces MTTi coding with neo2 upsteam in Mtt1 5’ flanking region
• ( SD02566 ) Macronucleus: BLT1with c-terminal HA replaces MTTi coding with neo2 upsteam in Mtt1 5’ flanking region
• ( SD02567 ) Macronucleus: BLT1with c-terminal HA replaces MTTi coding with neo2 upsteam in Mtt1 5’ flanking region

Homologs

No Data fetched for Homologs

General Information

No Data fetched for General Information

Associated Literature

1. Ref:16933976: Eisen JA, Coyne RS, Wu M, Wu D, Thiagarajan M, Wortman JR, Badger JH, Ren Q, Amedeo P, Jones KM, Tallon LJ, Delcher AL, Salzberg SL, Silva JC, Haas BJ, Majoros WH, Farzad M, Carlton JM, Smith RK, Garg J, Pearlman RE, Karrer KM, Sun L, Manning G, Elde NC, Turkewitz AP, Asai DJ, Wilkes DE, Wang Y, Cai H, Collins K, Stewart BA, Lee SR, Wilamowska K, Weinberg Z, Ruzzo WL, Wloga D, Gaertig J, Frankel J, Tsao CC, Gorovsky MA, Keeling PJ, Waller RF, Patron NJ, Cherry JM, Stover NA,
2. Ref:11891286: Shang Y, Song X, Bowen J, Corstanje R, Gao Y, Gaertig J, Gorovsky MA (2002) A robust inducible-repressible promoter greatly facilitates gene knockouts, conditional expression, and overexpression of homologous and heterologous genes in Tetrahymena thermophila. Proceedings of the National Academy of Sciences of the United States of America 99(6):3734-9

Sequences

>TTHERM_01104960(coding)
ATGAGAGAAATTGTTTGCATTTCAGCAGGTCAATGCGGTAACTAAATAGGATATAAGTTC
TGGGAAAGAGTTTCTCAAGAGCATGGAGTCGAAGAGACTGGTCTGTATAACGGAAATAAT
GACTTATAGCTCGAGAGAATTGATGTTTACTATAATGAAGGCTCAAGTGGACGTTATGTA
CCTAGATCAGTAATGGTTGATTTGGAACCTGGTGTATTGGATTCAATTCGTGCATCAAAA
TACAGATAACTTTTCCATCCTGATAATTTCATTCACGCTCAAAGTAGCGCTTCTAATAAT
TGGGCTAAGGGACACTATTCTGAAGGTGCTGAACTTTAATAGGAAGTTTTAGATATTGTC
CGTAGAGAAGTTGAAGGTTGTGACAGCCTATAAGGTTTCCAATTCACTCATTCTATTGGT
GGAGGCACTGGAAGTGGTATGGGCACTTTGCTTATTTAAAAGCTTAGAGAAGAATATCCA
GACAGAATTTTTTCAAGTTTTTCTGTTTTCCCATCCGTAAAAGTAAGCGATGTTGTAATT
GAACCTTATAATGCTACATTGAGCATTCATTAGCTCATAGAAAATGTTGACTAATGCATG
GTTATAGATAATGAAGCTTTATACAATATTTGCATGAAAAATTTAAAGATTGAACACCCT
ACTTATGGTGACCTCAATCATATTGTCTCTGTAGCTATGTCTGGTGTTACTTGCAGTATG
AGATTCCCTGGATAATTAAATGCAGATCTCAGAAAATTGTCAGTTAATTTGACTCCCTTC
CCTCGTTTACACTTCTTTACAGTTGGAATAGCTCCTTTATATGGAAGAGGATAGGCTTAG
TTTGCCAGTAAATATGATGTTCCTGAAATTCTTTAGCAAGCTTTTAATCCTCACAATATG
ATGTGTGCTGCCGATGTTTCTCATGGAAAATATTTAACAGCTGCTCTTGCTTTTAGAGGA
AAAGTATCATCATATGAAATCGAAAAGCAACTACTAAACTTCTAACAAAAAAATAGCAAC
CGTTTTACAGAATGGATTCCAAACAGTATGAAGCTAAGTATGTGCGATATATCCTTGGAA
TAAGTACCAAGAAGCGCTGTTTTAATAAGTAATAACACATCGATATAAGAAGTATTCAAA
AGAATAAATGCTTAATATACAAGCATGTTTAGAAGAAAGGCTTTCGTTCACTGGTATTTA
GATGAAGGTATGGACGAAATGGAGTTTAATGAAGCAGAAGCTAATATGAATGACTTGGTG
AGTGAATATCAATAATACTAAGATTCAACCGTAGACAGTGAAGAAGAAATTGACTTAGAT
TAGACATAGGCAACATTTGGAGAAAGATAAGAAAAACAAAATAAAAAATGTAACTCAAAA
TAACAATCTAATATTGCTTCATCAAATGAAATTTGA

>TTHERM_01104960(gene)
AGATTTGAAATTTTAATAAAAAAATTTTGATTAAAATACTAGTTAATACAAGTCCATTAT
TGAATAATTTAATTAATTCATAATAAATAAATCATAAGAAATAATATAAAAATAACATAA
GCTATTTTAACAAATAAAATAAAGTAATAACTCAACAAATGAGAGAAATTGTTTGCATTT
CAGCAGGTCAATGCGGTAACTAAATAGGATATAAGTTCTGGGAAAGAGTTTCTCAAGAGC
ATGGAGTCGAAGAGACTGGTCTGTATAACGGAAATAATGACTTATAGCTCGAGAGAATTG
ATGTTTACTATAATGAAGGCTCAAGTGGACGTTATGTACCTAGATCAGTAATGGTTGATT
TGGAACCTGGTGTATTGGATTCAATTCGTGCATCAAAATACAGATAACTTTTCCATCCTG
ATAATTTCATTCACGCTCAAAGTAGCGCTTCTAATAATTGGGCTAAGGGACACTATTCTG
AAGGTGCTGAACTTTAATAGGAAGTTTTAGATATTGTCCGTAGAGAAGTTGAAGGTTGTG
ACAGCCTATAAGGTTTCCAATTCACTCATTCTATTGGTGGAGGCACTGGAAGTGGTATGG
GCACTTTGCTTATTTAAAAGCTTAGAGAAGAATATCCAGACAGAATTTTTTCAAGTTTTT
CTGTTTTCCCATCCGTAAAAGTAAGCGATGTTGTAATTGAACCTTATAATGCTACATTGA
GCATTCATTAGCTCATAGAAAATGTTGACTAATGCATGGTTATAGATAATGAAGCTTTAT
ACAATATTTGCATGAAAAATTTAAAGATTGAACACCCTACTTATGGTGACCTCAATCATA
TTGTCTCTGTAGCTATGTCTGGTGTTACTTGCAGTATGAGATTCCCTGGATAATTAAATG
CAGATCTCAGAAAATTGTCAGTTAATTTGACTCCCTTCCCTCGTTTACACTTCTTTACAG
TTGGAATAGCTCCTTTATATGGAAGAGGATAGGCTTAGTTTGCCAGTAAATATGATGTTC
CTGAAATTCTTTAGCAAGCTTTTAATCCTCACAATATGATGTGTGCTGCCGATGTTTCTC
ATGGAAAATATTTAACAGCTGCTCTTGCTTTTAGAGGAAAAGTATCATCATATGAAATCG
AAAAGCAACTACTAAACTTCTAACAAAAAAATAGCAACCGTTTTACAGAATGGATTCCAA
ACAGTATGAAGCTAAGTATGTGCGATATATCCTTGGAATAAGTACCAAGAAGCGCTGTTT
TAATAAGTAATAACACATCGATATAAGAAGTATTCAAAAGAATAAATGCTTAATATACAA
GCATGTTTAGAAGAAAGGCTTTCGTTCACTGGTATTTAGATGAAGGTATGGACGAAATGG
AGTTTAATGAAGCAGAAGCTAATATGAATGACTTGGTGAGTGAATATCAATAATACTAAG
ATTCAACCGTAGACAGTGAAGAAGAAATTGACTTAGATTAGACATAGGCAACATTTGGAG
AAAGATAAGAAAAACAAAATAAAAAATGTAACTCAAAATAACAATCTAATATTGCTTCAT
CAAATGAAATTTGATGAATTTAACTATAAAATTTACTAAAATATATTATAATAAATAATT
AACAATGTACATAATATAAAACAAAATACTATTTAATAATAAATTTATAATTATATATTT
ATAAAAAATTCATT

>TTHERM_01104960(protein)
MREIVCISAGQCGNQIGYKFWERVSQEHGVEETGLYNGNNDLQLERIDVYYNEGSSGRYV
PRSVMVDLEPGVLDSIRASKYRQLFHPDNFIHAQSSASNNWAKGHYSEGAELQQEVLDIV
RREVEGCDSLQGFQFTHSIGGGTGSGMGTLLIQKLREEYPDRIFSSFSVFPSVKVSDVVI
EPYNATLSIHQLIENVDQCMVIDNEALYNICMKNLKIEHPTYGDLNHIVSVAMSGVTCSM
RFPGQLNADLRKLSVNLTPFPRLHFFTVGIAPLYGRGQAQFASKYDVPEILQQAFNPHNM
MCAADVSHGKYLTAALAFRGKVSSYEIEKQLLNFQQKNSNRFTEWIPNSMKLSMCDISLE
QVPRSAVLISNNTSIQEVFKRINAQYTSMFRRKAFVHWYLDEGMDEMEFNEAEANMNDLV
SEYQQYQDSTVDSEEEIDLDQTQATFGERQEKQNKKCNSKQQSNIASSNEI