Identifiers and Description

Gene Model Identifier

TTHERM_01107220

Standard Name

TPB2 (Tetrahymena PiggyBac transposase 2)

Aliases

PreTt28071 | 237.m00028 | 3794.m00164

Description

TPB2 PiggyBac-like protein Tpb2p; PiggyBac transposase, tpb2p; produces double-strand breaks with four-base 5' protruding ends on DNA; involved in DNA cleavage step of programmed DNA deletion; PiggyBac transposable element-derived protein

Genome Browser (Macronucleus)

Full Screen View

No Genome Browser Data Present

Genome Browser (Micronucleus)

Full Screen View

No Genome Browser Data Present

External Links

• Uniprot
• Alphafold

Gene Ontology Annotations

Cellular Component

• heterochromatin (IDA) | GO:0000792
• macronucleus (IDA) | GO:0031039

Molecular Function

• DNA endonuclease activity (IDA) | GO:0004520

Biological Process

• heterochromatin formation (IMP) | GO:0031507
• programmed DNA elimination (IMP) | GO:0031049

Domains

• ( PF13843 ) Transposase IS4

Gene Expression Profile

Vegetative Cell Cycle (Zhang et al., 2023)

GeneMania

No results were calculated for this gene in GeneMania.

Tetrahymena Stock Center

No Data fetched for Tetrahymena Stock Center

Homologs

No Data fetched for Homologs

General Information

No Data fetched for General Information

Associated Literature

1. Ref:31262823: Mutazono M, Noto T, Mochizuki K (2019) Diversification of small RNA amplification mechanisms for targeting transposon-related sequences in ciliates. Proceedings of the National Academy of Sciences of the United States of America ( ):
2. Ref:29021213: Noto T, Mochizuki K (2017) Whats, hows and whys of programmed DNA elimination in Tetrahymena. Open biology 7(10):
3. Ref:24348275: Vogt A, Mochizuki K (2013) A domesticated PiggyBac transposase interacts with heterochromatin and catalyzes reproducible DNA elimination in Tetrahymena. PLoS genetics 9(12):e1004032
4. Ref:23935529: Vogt A, Goldman AD, Mochizuki K, Landweber LF (2013) Transposon domestication versus mutualism in ciliate genome rearrangements. PLoS genetics 9(8):e1003659
5. Ref:20357003: Cheng CY, Vogt A, Mochizuki K, Yao MC (2010) A domesticated piggyBac transposase plays key roles in heterochromatin dynamics and DNA cleavage during programmed DNA deletion in Tetrahymena thermophila. Molecular biology of the cell 21(10):1753-62

Sequences

>TTHERM_01107220(coding)
ATGAAAAGAGATATTAACACGCCTATTGATAAAATGAAATTTTACTAGCCTTTAGATAAA
GAAGGCTAGCCTGTTGTAATGTATGACGATGGTACTATAAAAAAATTAGGACAAAAAAGA
ACTTATGAAGGTTAACCTGAATAGTATATCGTTAAAACAAAAAAGAACGATGTTTACTTA
GTTGAAAAATCAAAGAAAGTTAGAAAAGCTAGAAAAAGAAAATAATTTTAAGAAGGAGAA
GATGCTAAGAAACCTTTAACAGAAAAAGAAGTGGAAAAGAGATGGACTGAATTCTCTAAA
GAAAATAAAATGAGACAATAACATAAAGAAGATGATTTGATTGATGATCAATGGAGAAGA
ATATATTCTGTAGATTAAGTTCCAAAAAGCTTTTATATGAAAACTAAATTTGATAAAGAT
TTGAATAGAGATTTTATTCCATATAATGTACCTCATTCCCCATATCATCTGTTCAGAATG
TTTTTTGACGATAGAATATATAAATTGATTATTGAAGAAACTAACCGATATAAACATCAG
AAATATTAAGAGGCCTTACTAAATCTACCTCCTGATAAAACTCTTCCCAAAGAGGCTATG
GACATAGATTCTATAGATTTAGATGCATATCTTTCAATTTTGATATTTATGGGTGTGTAA
AGAATGAAAAGTGTTAAAGACTATTGGAAGAGGAAAAATTATATTAATAGTGAAATAGCG
TGCATAATGAAGTATTCTAGATTTGAATAAATAGACAAACATTTACATTTAGCAGATAAT
GAAGACCCTAAAATCAGAAGTGATCCTATTGGAAAAGTTAGATAATATATGGATTATTTA
AATGAAAATTTTAAGAAATACTATTACCCAGGAGAGTTCCTAGCTATTGATGAAGGTATG
ATACCTTTTAATGGCAAAGTCGCTTTCAAGGTCTATAATCCTGATAAACCCGATAAGTTT
GGTATCAAAGAATATGTTTGTTGTGATTCTTAAAACGCTTACACTTTAGAATCTTAGCTC
TATTATGGTAACCATGAAAATGAAGAATTCCCAGAAATTACTCTTTCCAAAACTAATGAA
GTTGTAATGAATTTACTAAAAGACTATGAAGGCAGATTTCATAAAGTAGTAATGGATAAT
TATTATAACAGTCCAACTTTGTTTTATCTGATGAAATAAAAATCTTTTGGTGCTCTAGGT
ACCATGAGAATCGGGCGTCTAAAATTACCTCCTTATTTACTCTCTTAAATTATGGAAGTC
CATCAAAATAAGGTTTTATCTTACATTTAAGGTGATATCAATCTGGCTATTTTCTTCAGA
GGAACAAACGATAAAGAAATTTGCCTAATGTCAAATTTCATAGATTAAAAAAAGGTTAAA
GATGATTTTTGGAGAGTTCTTGCGTACGTTAAAGAAGACAGAGATATTTTCAAAGAGTAA
AATTTATAGAGCGCTTATTATAACAAATACAAAGGAGGTGTTGATAGAAGAAACAGTTAT
CTCGCAGCATATAGAAATTGTAGGAAGAATATTAAATGGTATAGGCCCGTCTTTTATAGA
ATGTTAGATAATGCGATAGTTAATGCATTTATCCTTTATAATTTTAATTTAAGTCCTAAA
TATAAAATTTCACAAAAAGAATTTAGAATATAGCTATTTAAGGAACTTGCTTGCAGATAT
TCTCCATCTAAAAGCCAACACGCAATCAAAGTCCAATAATTTATGAAAGATAAATCTGCT
GAAGTGGATAAAATACCACAATATTTTGAAAAAGCTATGAAACTTCATATTATGAAAAAT
TGTGATTACTCAGCTCATATTCTTGTTAGATCCCGTACTAAAAAAAAATCTTGCATTGAA
TGCAAATAATTAACTTTATTTTCTTGCAGTACATGCTCAAATATGTTTAAGATGAGAATA
CCTCTTTGTTAATCAGGTTTCAACTAATGCTATGATTTCCATGCAAGTAAAACATATGAA
GAGGTTGTTGAAGATATGTTTACTAAGAGAAGAAAAGATGGAAGAAAATTAGATATCGAA
ATTAAAAAGAAAAAGAAAAAATTAGTTAAGCTCTCAGATGTTTATGATTCAATATTAAAG
CCAAAATAAAGCAATTAAAATCCAATACAAGTAAAAACTAAACAAATCCTATAAAAATCT
GTTACTATTGATCACTACACAAGCAATAAAGCATTTTTAGAAGATGATATGTATTCAGAA
AACTAACCTAATTCAACTGAATTAAATGATCAGTCTACTCGTATGAAGACAAACTAAATC
ATGAGCTCTTCTAATTCAAATTCAATATAAGGAAGTAAAAAAAAAAAGTTCAAGGTCATT
AAAAAGGAAAAAAATTTAAAAACTCAAAAGACAAAATCTTGTGATGCAATATTTAGCAGT
GAGAAGATAAAAAAGAAATATACTGATGAAATAAATGAATTTTTTGATTCAAAAGCTTCC
ATTAGGTTAACTAATTTCTTTTAAGAGAGCTAACGTCTTTAAAATTTACTGAAAGAATCA
TAAGAAAATCTTTCTAAAAAATTTGTTGTTTTAGATTAGACCAGTGATGAAGACTTTAAA
AATAATAACACAGACGAAGATCTCTAATGGATAAATACAACTTTAGAGAAAATAAATTAA
AAAAAAATAGAACCTAACGAAAAATTATCTCCTGGCCTTGGTATAATAAACAAAAAATAA
GAGGCAGAAGAAGTAATAGAAGTATTATCAAGCAGCTCTACTCTAAACGAAGAAAAAGTA
AGAACTAAATATTAAGAAATTCCTTTTACTTTTGGAAGCATTCTTGAAGTAGAACAAGAA
AAAGAAGAATCAGATGATGAAGTTGCGAAGGAATACAAAAAAGTTTCTAAAAGAATAGAA
GAATTTAATGAAGATTCCATTCATTCAAAATAACAACTAGAGGACAGCAATTTTTTTCCA
TAAAAAGCTGGAGTGATTTTTTACTCGATGAGTGAATTAGATAAAAAAAAAGAATAAGTA
ATAAGTGACTAAAATAAAGAATAGAATTAAATTATAGTTAAGTAAGAACATAGATCTGAC
TAGAAAAAAAAGAATAGCTATCCTCTATAACTTTATGACTGTGAAAATGTCCTGAACAAT
GCCATAGATTTTTCTGATTACTCATTTTCATCTTCTGATGAAGAACAATTTGTTTATAAT
AAAAATAAGAACAAATAACAAGAATTAAATCACGAATCAGATACAAATAATCCCTAACTA
TAATACAAAGGCAAAATAAAAAATAATAAATTTAAAATTGATGAGAGCTTTGATGAAAGC
AATCTTAAAATAGAAAGGCCTTTTGATTTAATTCCATTTACCTTTTAAAATGATGCAATG
TAAACTAGTTAAAATTTTGTAGTCATTGATGATGACAGTGATGTAGAATTTTAAAATATA
AATTCTTAGATAATAAATTAATCAAGCATAATTTCAGAAAGTTAAAAATTGGAATTTGAA
GGATTTATTGATAATCCATAACCAACTAAAAAAAGGATAATCTCTTGCCAAGACATGAAA
AGAAAAATTATTATTAAAAAAAATAAAGAGCCAAAAAATGATAATTAGAATATCATTTAC
ATTAACCATTAACGTTTTAAAATAGTGAAGCTAAAAACTACTATATAAATTGACTCAGAC
TGA

>TTHERM_01107220(gene)
TACTTATTTTAAAATGTTATTAAAAAAAATCTGAGCTACCTCTTTTTTTATAATTTAATA
TTAAAATTTTCTTTGATTTGTTATTACGTTTTAAAAAATGAAAAGAGATATTAACACGCC
TATTGATAAAATGAAATTTTACTAGCCTTTAGATAAAGAAGGCTAGCCTGTTGTAATGTA
TGACGATGGTACTATAAAAAAATTAGGACAAAAAAGAACTTATGAAGGTTAACCTGAATA
GTATATCGTTAAAACAAAAAAGAACGATGTTTACTTAGTTGAAAAATCAAAGAAAGTTAG
AAAAGCTAGAAAAAGAAAATAATTTTAAGAAGGAGAAGATGCTAAGAAACCTTTAACAGA
AAAAGAAGTGGAAAAGAGATGGACTGAATTCTCTAAAGAAAATAAAATGAGACAATAACA
TAAAGAAGATGATTTGATTGATGATCAATGGAGAAGAATATATTCTGTAGATTAAGTTCC
AAAAAGCTTTTATATGAAAACTAAATTTGATAAAGATTTGAATAGAGATTTTATTCCATA
TAATGTACCTCATTCCCCATATCATCTGTTCAGAATGTTTTTTGACGATAGAATATATAA
ATTGATTATTGAAGAAACTAACCGATATAAACATCAGAAATATTAAGAGGCCTTACTAAA
TCTACCTCCTGATAAAACTCTTCCCAAAGAGGCTATGGACATAGATTCTATAGATTTAGA
TGCATATCTTTCAATTTTGATATTTATGGGTGTGTAAAGAATGAAAAGTGTTAAAGACTA
TTGGAAGAGGAAAAATTATATTAATAGTGAAATAGCGTGCATAATGAAGTATTCTAGATT
TGAATAAATAGACAAACATTTACATTTAGCAGATAATGAAGACCCTAAAATCAGAAGTGA
TCCTATTGGAAAAGTTAGATAATATATGGATTATTTAAATGAAAATTTTAAGAAATACTA
TTACCCAGGAGAGTTCCTAGCTATTGATGAAGGTATGATACCTTTTAATGGCAAAGTCGC
TTTCAAGGTCTATAATCCTGATAAACCCGATAAGTTTGGTATCAAAGAATATGTTTGTTG
TGATTCTTAAAACGCTTACACTTTAGAATCTTAGCTCTATTATGGTAACCATGAAAATGA
AGAATTCCCAGAAATTACTCTTTCCAAAACTAATGAAGTTGTAATGAATTTACTAAAAGA
CTATGAAGGCAGATTTCATAAAGTAGTAATGGATAATTATTATAACAGTCCAACTTTGTT
TTATCTGATGAAATAAAAATCTTTTGGTGCTCTAGGTACCATGAGAATCGGGCGTCTAAA
ATTACCTCCTTATTTACTCTCTTAAATTATGGAAGTCCATCAAAATAAGGTTTTATCTTA
CATTTAAGGTGATATCAATCTGGCTATTTTCTTCAGAGGAACAAACGATAAAGAAATTTG
CCTAATGTCAAATTTCATAGATTAAAAAAAGGTTAAAGATGATTTTTGGAGAGTTCTTGC
GTACGTTAAAGAAGACAGAGATATTTTCAAAGAGTAAAATTTATAGAGCGCTTATTATAA
CAAATACAAAGGAGGTGTTGATAGAAGAAACAGTTATCTCGCAGCATATAGAAATTGTAG
GAAGAATATTAAATGGTATAGGCCCGTCTTTTATAGAATGTTAGATAATGCGATAGTTAA
TGCATTTATCCTTTATAATTTTAATTTAAGTCCTAAATATAAAATTTCACAAAAAGAATT
TAGAATATAGCTATTTAAGGAACTTGCTTGCAGATATTCTCCATCTAAAAGCCAACACGC
AATCAAAGTCCAATAATTTATGAAAGATAAATCTGCTGAAGTGGATAAAATACCACAATA
TTTTGAAAAAGCTATGAAACTTCATATTATGAAAAATTGTGATTACTCAGCTCATATTCT
TGTTAGATCCCGTACTAAAAAAAAATCTTGCATTGAATGCAAATAATTAACTTTATTTTC
TTGCAGTACATGCTCAAATATGTTTAAGATGAGAATACCTCTTTGTTAATCAGGTTTCAA
CTAATGCTATGATTTCCATGCAAGTAAAACATATGAAGAGGTTGTTGAAGATATGTTTAC
TAAGAGAAGAAAAGATGGAAGAAAATTAGATATCGAAATTAAAAAGAAAAAGAAAAAATT
AGTTAAGCTCTCAGATGTTTATGATTCAATATTAAAGCCAAAATAAAGCAATTAAAATCC
AATACAAGTAAAAACTAAACAAATCCTATAAAAATCTGTTACTATTGATCACTACACAAG
CAATAAAGCATTTTTAGAAGATGATATGTATTCAGAAAACTAACCTAATTCAACTGAATT
AAATGATCAGTCTACTCGTATGAAGACAAACTAAATCATGAGCTCTTCTAATTCAAATTC
AATATAAGGAAGTAAAAAAAAAAAGTTCAAGGTCATTAAAAAGGAAAAAAATTTAAAAAC
TCAAAAGACAAAATCTTGTGATGCAATATTTAGCAGTGAGAAGATAAAAAAGAAATATAC
TGATGAAATAAATGAATTTTTTGATTCAAAAGCTTCCATTAGGTTAACTAATTTCTTTTA
AGAGAGCTAACGTCTTTAAAATTTACTGAAAGAATCATAAGAAAATCTTTCTAAAAAATT
TGTTGTTTTAGATTAGACCAGTGATGAAGACTTTAAAAATAATAACACAGACGAAGATCT
CTAATGGATAAATACAACTTTAGAGAAAATAAATTAAAAAAAAATAGAACCTAACGAAAA
ATTATCTCCTGGCCTTGGTATAATAAACAAAAAATAAGAGGCAGAAGAAGTAATAGAAGT
ATTATCAAGCAGCTCTACTCTAAACGAAGAAAAAGTAAGAACTAAATATTAAGAAATTCC
TTTTACTTTTGGAAGCATTCTTGAAGTAGAACAAGAAAAAGAAGAATCAGATGATGAAGT
TGCGAAGGAATACAAAAAAGTTTCTAAAAGAATAGAAGAATTTAATGAAGATTCCATTCA
TTCAAAATAACAACTAGAGGACAGCAATTTTTTTCCATAAAAAGCTGGAGTGATTTTTTA
CTCGATGAGTGAATTAGATAAAAAAAAAGAATAAGTAATAAGTGACTAAAATAAAGAATA
GAATTAAATTATAGTTAAGTAAGAACATAGATCTGACTAGAAAAAAAAGAATAGCTATCC
TCTATAACTTTATGACTGTGAAAATGTCCTGAACAATGCCATAGATTTTTCTGATTACTC
ATTTTCATCTTCTGATGAAGAACAATTTGTTTATAATAAAAATAAGAACAAATAACAAGA
ATTAAATCACGAATCAGATACAAATAATCCCTAACTATAATACAAAGGCAAAATAAAAAA
TAATAAATTTAAAATTGATGAGAGCTTTGATGAAAGCAATCTTAAAATAGAAAGGCCTTT
TGATTTAATTCCATTTACCTTTTAAAATGATGCAATGTAAACTAGTTAAAATTTTGTAGT
CATTGATGATGACAGTGATGTAGAATTTTAAAATATAAATTCTTAGATAATAAATTAATC
AAGCATAATTTCAGAAAGTTAAAAATTGGAATTTGAAGGATTTATTGATAATCCATAACC
AACTAAAAAAAGGATAATCTCTTGCCAAGACATGAAAAGAAAAATTATTATTAAAAAAAA
TAAAGAGCCAAAAAATGATAATTAGAATATCATTTACATTAACCATTAACGTTTTAAAAT
AGTGAAGCTAAAAACTACTATATAAATTGACTCAGACTGAAAGCTCACTAATTTTATCAT
CAATCATTATGTAAAAATCAAAATTATGTTGAATATCTTTTTACTTGTATAAACAGTTCT
ATTTTTTAGTTTGTAAATGTATTATCTTATCAGTTATTTTTTTGGCATAAAATAAAATTA
AAATATTTGAGAAAAACCATCACAAA

>TTHERM_01107220(protein)
MKRDINTPIDKMKFYQPLDKEGQPVVMYDDGTIKKLGQKRTYEGQPEQYIVKTKKNDVYL
VEKSKKVRKARKRKQFQEGEDAKKPLTEKEVEKRWTEFSKENKMRQQHKEDDLIDDQWRR
IYSVDQVPKSFYMKTKFDKDLNRDFIPYNVPHSPYHLFRMFFDDRIYKLIIEETNRYKHQ
KYQEALLNLPPDKTLPKEAMDIDSIDLDAYLSILIFMGVQRMKSVKDYWKRKNYINSEIA
CIMKYSRFEQIDKHLHLADNEDPKIRSDPIGKVRQYMDYLNENFKKYYYPGEFLAIDEGM
IPFNGKVAFKVYNPDKPDKFGIKEYVCCDSQNAYTLESQLYYGNHENEEFPEITLSKTNE
VVMNLLKDYEGRFHKVVMDNYYNSPTLFYLMKQKSFGALGTMRIGRLKLPPYLLSQIMEV
HQNKVLSYIQGDINLAIFFRGTNDKEICLMSNFIDQKKVKDDFWRVLAYVKEDRDIFKEQ
NLQSAYYNKYKGGVDRRNSYLAAYRNCRKNIKWYRPVFYRMLDNAIVNAFILYNFNLSPK
YKISQKEFRIQLFKELACRYSPSKSQHAIKVQQFMKDKSAEVDKIPQYFEKAMKLHIMKN
CDYSAHILVRSRTKKKSCIECKQLTLFSCSTCSNMFKMRIPLCQSGFNQCYDFHASKTYE
EVVEDMFTKRRKDGRKLDIEIKKKKKKLVKLSDVYDSILKPKQSNQNPIQVKTKQILQKS
VTIDHYTSNKAFLEDDMYSENQPNSTELNDQSTRMKTNQIMSSSNSNSIQGSKKKKFKVI
KKEKNLKTQKTKSCDAIFSSEKIKKKYTDEINEFFDSKASIRLTNFFQESQRLQNLLKES
QENLSKKFVVLDQTSDEDFKNNNTDEDLQWINTTLEKINQKKIEPNEKLSPGLGIINKKQ
EAEEVIEVLSSSSTLNEEKVRTKYQEIPFTFGSILEVEQEKEESDDEVAKEYKKVSKRIE
EFNEDSIHSKQQLEDSNFFPQKAGVIFYSMSELDKKKEQVISDQNKEQNQIIVKQEHRSD
QKKKNSYPLQLYDCENVLNNAIDFSDYSFSSSDEEQFVYNKNKNKQQELNHESDTNNPQL
QYKGKIKNNKFKIDESFDESNLKIERPFDLIPFTFQNDAMQTSQNFVVIDDDSDVEFQNI
NSQIINQSSIISESQKLEFEGFIDNPQPTKKRIISCQDMKRKIIIKKNKEPKNDNQNIIY
INHQRFKIVKLKTTIQIDSD