Identifiers and Description

Gene Model Identifier

TTHERM_01107220

Standard Name

TPB2 (Tetrahymena PiggyBac transposase 2)

Aliases

PreTt28071 | 237.m00028 | 3794.m00164

Description

TPB2 PiggyBac-like protein Tpb2p; PiggyBac transposase- tpb2p; produces double-strand breaks with four-base 5' protruding ends on DNA; involved in DNA cleavage step of programmed DNA deletion

Genome Browser (Macronucleus)

Full Screen View

No Genome Browser Data Present

Genome Browser (Micronucleus)

Full Screen View

No Genome Browser Data Present

External Links

• Uniprot
• Alphafold

Gene Ontology Annotations

Cellular Component

• heterochromatin (IDA) | GO:0000792
• macronucleus (IDA) | GO:0031039

Molecular Function

• DNA endonuclease activity (IDA) | GO:0004520

Biological Process

• heterochromatin formation (IMP) | GO:0031507
• programmed DNA elimination (IMP) | GO:0031049

Domains

No Data fetched for Domains

Gene Expression Profile

Vegetative Cell Cycle (Zhang et al., 2023)

GeneMania

No results were calculated for this gene in GeneMania.

Tetrahymena Stock Center

No Data fetched for Tetrahymena Stock Center

Homologs

Source	Identifier	Score
Tetrahymena borealis	EI9_20298.1	4.0002634815779907e-110
	Description: hypothetical protein (509 aa)
WormBase	WBGene00018014	4.0002634815779907e-110
	Description: status:Confirmed UniProt:Q19986 protein_id:CCD65791.1

General Information

No Data fetched for General Information

Associated Literature

1. Ref:31262823: Mutazono M, Noto T, Mochizuki K (2019) Diversification of small RNA amplification mechanisms for targeting transposon-related sequences in ciliates. Proceedings of the National Academy of Sciences of the United States of America ( ):
2. Ref:29021213: Noto T, Mochizuki K (2017) Whats, hows and whys of programmed DNA elimination in Tetrahymena. Open biology 7(10):
3. Ref:24348275: Vogt A, Mochizuki K (2013) A domesticated PiggyBac transposase interacts with heterochromatin and catalyzes reproducible DNA elimination in Tetrahymena. PLoS genetics 9(12):e1004032
4. Ref:23935529: Vogt A, Goldman AD, Mochizuki K, Landweber LF (2013) Transposon domestication versus mutualism in ciliate genome rearrangements. PLoS genetics 9(8):e1003659
5. Ref:20357003: Cheng CY, Vogt A, Mochizuki K, Yao MC (2010) A domesticated piggyBac transposase plays key roles in heterochromatin dynamics and DNA cleavage during programmed DNA deletion in Tetrahymena thermophila. Molecular biology of the cell 21(10):1753-62

Sequences

>TTHERM_01107220(coding)
ATGAAAAGAGATATTAACACGCCTATTGATAAAATGAAATTTTACTAGCCTTTAGATAAA
GAAGGCTAGCCTGTTGTAATGTATGACGATGGTACTATAAAAAAATTAGGACAAAAAAGA
ACTTATGAAGGTTAACCTGAATAGTATATCGTTAAAACAAAAAAGAACGATGTTTACTTA
GTTGAAAAATCAAAGAAAGTTAGAAAAGCTAGAAAAAGAAAATAATTTTAAGAAGGAGAA
GATGCTAAGAAACCTTTAACAGAAAAAGAAGTGGAAAAGAGATGGACTGAATTCTCTAAA
GAAAATAAAATGAGACAATAACATAAAGAAGATGATTTGATTGATGATCAATGGAGAAGA
ATATATTCTGTAGATTAAGTTCCAAAAAGCTTTTATATGAAAACTAAATTTGATAAAGAT
TTGAATAGAGATTTTATTCCATATAATGTACCTCATTCCCCATATCATCTGTTCAGAATG
TTTTTTGACGATAGAATATATAAATTGATTATTGAAGAAACTAACCGATATAAACATCAG
AAATATTAAGAGGCCTTACTAAATCTACCTCCTGATAAAACTCTTCCCAAAGAGGCTATG
GACATAGATTCTATAGATTTAGATGCATATCTTTCAATTTTGATATTTATGGGTGTGTAA
AGAATGAAAAGTGTTAAAGACTATTGGAAGAGGAAAAATTATATTAATAGTGAAATAGCG
TGCATAATGAAGTATTCTAGATTTGAATAAATAGACAAACATTTACATTTAGCAGATAAT
GAAGACCCTAAAATCAGAAGTGATCCTATTGGAAAAGTTAGATAATATATGGATTATTTA
AATGAAAATTTTAAGAAATACTATTACCCAGGAGAGTTCCTAGCTATTGATGAAGGTATG
ATACCTTTTAATGGCAAAGTCGCTTTCAAGGTCTATAATCCTGATAAACCCGATAAGTTT
GGTATCAAAGAATATGTTTGTTGTGATTCTTAAAACGCTTACACTTTAGAATCTTAGCTC
TATTATGGTAACCATGAAAATGAAGAATTCCCAGAAATTACTCTTTCCAAAACTAATGAA
GTTGTAATGAATTTACTAAAAGACTATGAAGGCAGATTTCATAAAGTAGTAATGGATAAT
TATTATAACAGTCCAACTTTGTTTTATCTGATGAAATAAAAATCTTTTGGTGCTCTAGGT
ACCATGAGAATCGGGCGTCTAAAATTACCTCCTTATTTACTCTCTTAAATTATGGAAGTC
CATCAAAATAAGGTTTTATCTTACATTTAAGGTGATATCAATCTGGCTATTTTCTTCAGA
GGAACAAACGATAAAGAAATTTGCCTAATGTCAAATTTCATAGATTAAAAAAAGGTTAAA
GATGATTTTTGGAGAGTTCTTGCGTACGTTAAAGAAGACAGAGATATTTTCAAAGAGTAA
AATTTATAGAGCGCTTATTATAACAAATACAAAGGAGGTGTTGATAGAAGAAACAGTTAT
CTCGCAGCATATAGAAATTGTAGGAAGAATATTAAATGGTATAGGCCCGTCTTTTATAGA
ATGTTAGATAATGCGATAGTTAATGCATTTATCCTTTATAATTTTAATTTAAGTCCTAAA
TATAAAATTTCACAAAAAGAATTTAGAATATAGCTATTTAAGGAACTTGCTTGCAGATAT
TCTCCATCTAAAAGCCAACACGCAATCAAAGTCCAATAATTTATGAAAGATAAATCTGCT
GAAGTGGATAAAATACCACAATATTTTGAAAAAGCTATGAAACTTCATATTATGAAAAAT
TGTGATTACTCAGCTCATATTCTTGTTAGATCCCGTACTAAAAAAAAATCTTGCATTGAA
TGCAAATAATTAACTTTATTTTCTTGCAGTACATGCTCAAATATGTTTAAGATGAGAATA
CCTCTTTGTTAATCAGGTTTCAACTAATGCTATGATTTCCATGCAAGTAAAACATATGAA
GAGGTTGTTGAAGATATGTTTACTAAGAGAAGAAAAGATGGAAGAAAATTAGATATCGAA
ATTAAAAAGAAAAAGAAAAAATTAGTTAAGCTCTCAGATGTTTATGATTCAATATTAAAG
CCAAAATAAAGCAATTAAAATCCAATACAAGTAAAAACTAAACAAATCCTATAAAAATCT
GTTACTATTGATCACTACACAAGCAATAAAGCATTTTTAGAAGATGATATGTATTCAGAA
AACTAACCTAATTCAACTGAATTAAATGATCAGTCTACTCGTATGAAGACAAACTAAATC
ATGAGCTCTTCTAATTCAAATTCAATATAAGGAAGTAAAAAAAAAAAGTTCAAGGTCATT
AAAAAGGAAAAAAATTTAAAAACTCAAAAGACAAAATCTTGTGATGCAATATTTAGCAGT
GAGAAGATAAAAAAGAAATATACTGATGAAATAAATGAATTTTTTGATTCAAAAGCTTCC
ATTAGGTTAACTAATTTCTTTTAAGAGAGCTAACGTCTTTAAAATTTACTGAAAGAATCA
TAAGAAAATCTTTCTAAAAAATTTGTTGTTTTAGATTAGACCAGTGATGAAGACTTTAAA
AATAATAACACAGACGAAGATCTCTAATGGATAAATACAACTTTAGAGAAAATAAATTAA
AAAAAAATAGAACCTAACGAAAAATTATCTCCTGGCCTTGGTATAATAAACAAAAAATAA
GAGGCAGAAGAAGTAATAGAAGTATTATCAAGCAGCTCTACTCTAAACGAAGAAAAAGTA
AGAACTAAATATTAAGAAATTCCTTTTACTTTTGGAAGCATTCTTGAAGTAGAACAAGAA
AAAGAAGAATCAGATGATGAAGTTGCGAAGGAATACAAAAAAGTTTCTAAAAGAATAGAA
GAATTTAATGAAGATTCCATTCATTCAAAATAACAACTAGAGGACAGCAATTTTTTTCCA
TAAAAAGCTGGAGTGATTTTTTACTCGATGAGTGAATTAGATAAAAAAAAAGAATAAGTA
ATAAGTGACTAAAATAAAGAATAGAATTAAATTATAGTTAAGTAAGAACATAGATCTGAC
TAGAAAAAAAAGAATAGCTATCCTCTATAACTTTATGACTGTGAAAATGTCCTGAACAAT
GCCATAGATTTTTCTGATTACTCATTTTCATCTTCTGATGAAGAACAATTTGTTTATAAT
AAAAATAAGAACAAATAACAAGAATTAAATCACGAATCAGATACAAATAATCCCTAACTA
TAATACAAAGGCAAAATAAAAAATAATAAATTTAAAATTGATGAGAGCTTTGATGAAAGC
AATCTTAAAATAGAAAGGCCTTTTGATTTAATTCCATTTACCTTTTAAAATGATGCAATG
TAAACTAGTTAAAATTTTGTAGTCATTGATGATGACAGTGATGTAGAATTTTAAAATATA
AATTCTTAGATAATAAATTAATCAAGCATAATTTCAGAAAGTTAAAAATTGGAATTTGAA
GGATTTATTGATAATCCATAACCAACTAAAAAAAGGATAATCTCTTGCCAAGACATGAAA
AGAAAAATTATTATTAAAAAAAATAAAGAGCCAAAAAATGATAATTAGAATATCATTTAC
ATTAACCATTAACGTTTTAAAATAGTGAAGCTAAAAACTACTATATAAATTGACTCAGAC
TGA

>TTHERM_01107220(gene)
ATGAAAAGAGATATTAACACGCCTATTGATAAAATGAAATTTTACTAGCCTTTAGATAAA
GAAGGCTAGCCTGTTGTAATGTATGACGATGGTACTATAAAAAAATTAGGACAAAAAAGA
ACTTATGAAGGTAATTCTTAATTTTTAAAAATTAAATTTACTCATATTTATTAAAATATA
GGTTAACCTGAATAGTATATCGTTAAAACAAAAAAGAACGATGTTTACTTAGTTGAAAAA
TCAAAGAAAGTTAGAAAAGCTAGAAAAAGAAAATAATTTTAAGAAGGAGAAGATGCTAAG
AAACCTTTAACAGGTAAAATTTTTTTAGTATGGCAATTTAAATTAAAGTGTACCATTAAC
TATAGAAAAAGAAGTGGAAAAGAGATGGACTGAATTCTCTAAAGAAAATAAAGTAAAAAT
AATTATTAGTAAATTTTAAAGTAAAGCTATTTTATAAATACAGATGAGACAATAACATAA
AGAAGATGATTTGATTGATGATCAATGGAGAAGAATATATTCTGTAGATTAAGTTCCAAA
AAGCTTTTATATGAAAACTAAATTTGATAAAGATTTGAATAGAGTAAACGTTTTTTTTCT
CATTTTAAATTAAAATTTAACTTGACATTTAAATAAATTAGGATTTTATTCCATATAATG
TACCTCATTCCCCATATCATCTGTTCAGAATGTTTTTTGACGATAGAATATATAAATTGA
TTATTGAAGAAACTAACCGATATAAACATCAGAAATATTAAGAGGCCTTACTAAATCTAC
CTCCTGATAAAACTCTTCCCAAAGAGGCTATGGACATAGATTCTATAGATTTAGATGCAT
ATCTTTCAATTTTGATATTTATGGGTGTGTAAAGAATGAAAAGTGTTAAAGGTAAATAAT
TTTTTTATTACTTATTTATGACCTTTCAAAAATTTAATTAGAGACTTTTTAAAAATATTG
TCCATGCTTTTCTGAATAAAAATTTTAATGCAAAATTAGACTATTGGAAGAGGAAAAATT
ATATTAATAGTGAAATAGCGTGCATAATGAAGTATTCTAGATTTGAATAAATAGACAAAC
ATTTACATTTAGCAGATAATGAAGACCCTAAAATCAGAAGTGATCCTATTGGAAAAGTTA
GATAATATATGGATTATTTAAATGAAAATTTTAAGAAATACTATTACCCAGGAGAGTTCC
TAGCTATTGATGAAGGTATGATACCTTTTAATGGCAAAGTCGCTTTCAAGGTCTATAATC
CTGATAAACCCGATAAGGTATACTTTTATCATTTTTATTTATTGTTTTAAAAAATTTTTT
CTAGTTTGGTATCAAAGAATATGTTTGTTGTGATTCTTAAAACGCTTACACTTTAGAATC
TTAGCTCTATTATGGTAACCATGAAAATGAAGAATTCCCAGAAATTACTCTTTCCAAAAC
TAATGAAGGTTTTTTTAGTTTTTTCGAAATCAGTTTTGAAATTAATATTTATTATTAAAA
GTTGTAATGAATTTACTAAAAGACTATGAAGGCAGATTTCATAAAGTAGTAATGGATAAT
TATTATAACAGTCCAACTTTGTTTTATCTGATGAAATAAAAATCTTTTGGTGCTCTAGGT
ACCATGAGAATCGGGCGTCTAAAATTACCTCCTTATTTACTCTCTTAAATTATGGAAGTC
CATCAAAATAAGGTTTTAAATTATTTCTAAGTTGTGTATAAAATAACAAACTTAAAAAAA
TATATAAAAGGTTTTATCTTACATTTAAGGTGATATCAATCTGGCTATTTTCTTCAGAGG
AACAAACGATAAAGAAATTTGCCTAATGTCAAATTTCATAGATTAAAAAAAGGTTAAAGA
TGATGTAAATGTTTTCAAAATTTTCTTTCTCTTACTCATTGATTTTAAAAATAGTTTTGG
AGAGTTCTTGCGTACGTTAAAGAAGACAGAGATATTTTCAAAGAGTAAAATTTATAGGTA
AAAAAATTTTATTAAATGATAATTTTTATTTATTTTAAATCTTATTTTGATTAAATTTAT
CATGAAAAATGCAAATACAACCAAATAAGCTAATAGCTTAAGTTATAAGAAATAAAAATA
GCAATATTATTTTTAAATACCTAAATTTAGAGCGCTTATTATAACAAATACAAAGGAGGT
GTTGATAGAAGAAACAGTTATCTCGCAGCATATAGAAATTGTAGGAAGAATATTAAATGG
TATAGGCCCGTCTTTTATAGAATGTTAGATAATGCGATAGTTAATGCATTTATCCTTTAT
AATTTTAATTTAAGTCCTAAATATAAAATTTCACAAAAAGAATTTAGAATATAGCTATTT
AAGGAACTTGCTTGCAGGTAAAATTTTAATTATAAGAATTTTTTATTAATTTTATATAAA
AACATTAGATATTCTCCATCTAAAAGCCAACACGCAATCAAAGTCCAATAATTTATGAAA
GATAAATCTGCTGAAGTGGATAAAATACCACAATATTTTGAAAAAGCTATGAAACTTCAT
ATTATGAAAAATTGTGATTACTCAGCTCATATTCTTGTTAGATCCCGTACTAAAAAAAAA
TCTTGCATTGAATGCAAATAATTAACTTTATTTTCTTGCAGTACATGCTCAAATATGTTT
AAGATGAGAATACCTCTTTGTTAATCAGGTTTCAACTAAGTAAATTAATAAATATTTAAT
AGTAAATTAAATTACATTCATTAAATTACAGTGCTATGATTTCCATGCAAGTAAAACATA
TGAAGAGGTTGTTGAAGATATGTTTACTAAGAGAAGAAAAGATGGAAGAAAATTAGATAT
CGAAATTAAAAAGAAAAAGAAAAAATTAGTTAAGCTCTCAGATGTTTATGATTCAATATT
AAAGCCAAAATAAAGCAATTAAAATCCAATACAAGTAAAAACTAAACAAATCCTATAAAA
ATCTGTTACTATTGATCACTACACAAGCAATAAAGCATTTTTAGAAGATGATATGTATTC
AGAAAACTAACCTAATTCAACTGAATTAAATGATCAGTCTACTCGTATGAAGACAAACTA
AATCATGAGCTCTTCTAATTCAAATTCAATATAAGGTTATATTTTTCGAAAATTTTTATT
GAAATTTCATAAAATGTTTTATGAACAGGAAGTAAAAAAAAAAAGTTCAAGGTCATTAAA
AAGGAAAAAAATTTAAAAACTCAAAAGACAAAATCTTGTGATGCAATATTTAGCAGTGAG
AAGATAAAAAAGAAATATACTGATGAAATAAATGAATTTTTTGATTCAAAAGCTTCCATT
AGGTTAACTAATTTCTTTTAAGAGAGCTAACGTCTTTAAAATTTACTGAAAGAATCATAA
GAAAATCTTTCTAAAAAATTTGTTGTTTTAGATTAGACCAGTGATGAAGACTTTAAAAAT
AATAACACAGACGAAGATCTCTAATGGATAAATACAACTTTAGAGAAAATAAATTAAAAA
AAAATAGAACCTAACGAAAAATTATCTCCTGGCCTTGGTATAATAAACAAAAAATAAGAG
GCAGAAGAAGTAATAGAAGTATTATCAAGCAGCTCTACTCTAAACGAAGAAAAAGTAAGA
ACTAAATATTAAGAAATTCCTTTTACTTTTGGAAGCATTCTTGAAGTAGAACAAGAAAAA
GAAGAATCAGATGATGAAGTTGCGAAGGAATACAAAAAAGTTTCTAAAAGAATAGAAGAA
TTTAATGAAGATTCCATTCATTCAAAATAACAACTAGAGGACAGCAATTTTTTTCCATAA
AAAGCTGGAGTGATTTTTTACTCGATGAGTGAATTAGATAAAAAAAAAGAATAAGTAATA
AGTGACTAAAATAAAGAATAGAATTAAATTATAGTTAAGTAAGAACATAGATCTGACTAG
AAAAAAAAGAATAGCTATCCTCTATAACTTTATGACTGTGAAAATGTCCTGAACAATGCC
ATAGATTTTTCTGATTACTCATTTTCATCTTCTGATGAAGAACAATTTGTTTATAATAAA
AATAAGGTAAAATACTTTTAAATAAATTGTCAAATAAATATTTTTAATAAAATTTTATAG
AACAAATAACAAGAATTAAATCACGAATCAGATACAAATAATCCCTAACTATAATACAAA
GGCAAAATAAAAAATAATAAATTTAAAATTGATGAGAGCTTTGATGAAAGCAATCTTAAA
ATAGAAAGGCCTTTTGATTTAATTCCATTTACCTTTTAAAATGATGCAATGTAAACTAGT
TAAAATTTTGTAGTCATTGATGATGACAGTGATGTAGAATTTTAAAATATAAATTCTTAG
ATAATAAATTAATCAAGCATAATTTCAGAAAGTTAAAAATTGGAATTTGAAGGATTTATT
GATAATCCATAACCAACTAAAAAAAGGATAATCTCTTGCCAAGACATGAAAAGAAAAATT
ATTATTAAAAAAAATAAAGAGCCAAAAAATGATAATTAGAATATCATTTACATTAACCAT
TAACGTTTTAAAATAGTGAAGCTAAAAACTACTATATAAATTGACTCAGACTGA

>TTHERM_01107220(protein)
MKRDINTPIDKMKFYQPLDKEGQPVVMYDDGTIKKLGQKRTYEGQPEQYIVKTKKNDVYL
VEKSKKVRKARKRKQFQEGEDAKKPLTEKEVEKRWTEFSKENKMRQQHKEDDLIDDQWRR
IYSVDQVPKSFYMKTKFDKDLNRDFIPYNVPHSPYHLFRMFFDDRIYKLIIEETNRYKHQ
KYQEALLNLPPDKTLPKEAMDIDSIDLDAYLSILIFMGVQRMKSVKDYWKRKNYINSEIA
CIMKYSRFEQIDKHLHLADNEDPKIRSDPIGKVRQYMDYLNENFKKYYYPGEFLAIDEGM
IPFNGKVAFKVYNPDKPDKFGIKEYVCCDSQNAYTLESQLYYGNHENEEFPEITLSKTNE
VVMNLLKDYEGRFHKVVMDNYYNSPTLFYLMKQKSFGALGTMRIGRLKLPPYLLSQIMEV
HQNKVLSYIQGDINLAIFFRGTNDKEICLMSNFIDQKKVKDDFWRVLAYVKEDRDIFKEQ
NLQSAYYNKYKGGVDRRNSYLAAYRNCRKNIKWYRPVFYRMLDNAIVNAFILYNFNLSPK
YKISQKEFRIQLFKELACRYSPSKSQHAIKVQQFMKDKSAEVDKIPQYFEKAMKLHIMKN
CDYSAHILVRSRTKKKSCIECKQLTLFSCSTCSNMFKMRIPLCQSGFNQCYDFHASKTYE
EVVEDMFTKRRKDGRKLDIEIKKKKKKLVKLSDVYDSILKPKQSNQNPIQVKTKQILQKS
VTIDHYTSNKAFLEDDMYSENQPNSTELNDQSTRMKTNQIMSSSNSNSIQGSKKKKFKVI
KKEKNLKTQKTKSCDAIFSSEKIKKKYTDEINEFFDSKASIRLTNFFQESQRLQNLLKES
QENLSKKFVVLDQTSDEDFKNNNTDEDLQWINTTLEKINQKKIEPNEKLSPGLGIINKKQ
EAEEVIEVLSSSSTLNEEKVRTKYQEIPFTFGSILEVEQEKEESDDEVAKEYKKVSKRIE
EFNEDSIHSKQQLEDSNFFPQKAGVIFYSMSELDKKKEQVISDQNKEQNQIIVKQEHRSD
QKKKNSYPLQLYDCENVLNNAIDFSDYSFSSSDEEQFVYNKNKNKQQELNHESDTNNPQL
QYKGKIKNNKFKIDESFDESNLKIERPFDLIPFTFQNDAMQTSQNFVVIDDDSDVEFQNI
NSQIINQSSIISESQKLEFEGFIDNPQPTKKRIISCQDMKRKIIIKKNKEPKNDNQNIIY
INHQRFKIVKLKTTIQIDSD