Identifiers and Description

Gene Model Identifier

TTHERM_01276320

Standard Name

GIW1 (Gentleman-In-Waiting 1)

Aliases

PreTt28295 | 304.m00015 | COI1

Description

GIW1 Twi1p-associated protein; hypothetical protein

Genome Browser (Macronucleus)

Full Screen View

No Genome Browser Data Present

Genome Browser (Micronucleus)

Full Screen View

No Genome Browser Data Present

External Links

• Uniprot
• Alphafold

Gene Ontology Annotations

Cellular Component

• ARC complex (IDA) | GO:0033167
• cytoplasm (IDA) | GO:0005737
• macronucleus (IDA) | GO:0031039

Biological Process

• heterochromatin assembly involved in chromatin silencing by small RNA (IMP) | GO:0070924
• import into nucleus (IMP) | GO:0051170
• programmed DNA elimination (IMP) | GO:0031049

Domains

No Data fetched for Domains

Gene Expression Profile

Vegetative Cell Cycle (Zhang et al., 2023)

GeneMania

No results were calculated for this gene in GeneMania.

Tetrahymena Stock Center

• ( SD01818 ) Macronucleus: All GIW1 genes replaced by c-terminal HA tagged version with neo3 in 3’ flanking region
• ( SD01819 ) Macronucleus: All GIW1 genes replaced by c-terminal HA tagged version with neo3 in 3’ flanking region
• ( SD01820 ) Macronucleus: KO of GIW1 with neo3
• ( SD01821 ) Macronucleus: KO of GIW1 with neo3
• ( SD01822 ) Macronucleus: KO of GIW1 with neo3
• ( SD01823 ) Macronucleus: KO of GIW1 with neo3
• ( SD01824 ) Macronucleus: All GIW1 genes replaced by c-terminal V5 tagged version with neo3 in 3' flanking region
• ( SD01825 ) Macronucleus: All GIW1 genes replaced by c-terminal V5tagged version with neo3 in 3’ flanking region
• ( SD01826 ) Macronucleus: All GIW1 genes replaced by c-terminal V5 tagged version with neo3 in 3’ flanking region
• ( SD01827 ) Macronucleus: All GIW1 genes replaced by c-terminal V5 tagged version with neo3 in 3’ flanking region

Homologs

No Data fetched for Homologs

General Information

No.	Gene Name(s)	Paragraph Text
2311	COI12, GIW1	ATP-dependent and ATP-independent activities of Coi12p facilitate scnRNA loading by counteracting the Twi1p-binding protein Giw1p, which is important for sorting scnRNAs to Twi1p (Woehrer et al. 2015).
2310	TWI1, GIW1	Giw1p binds to Twi1p complexed with single-stranded, but not double-stranded, scnRNAs and that this interaction selectively promotes the MAC localization of the mature Twi1p-scnRNA complex (Noto et al. 2010).
2311	TWI1	ATP-dependent and ATP-independent activities of Coi12p facilitate scnRNA loading by counteracting the Twi1p-binding protein Giw1p, which is important for sorting scnRNAs to Twi1p (Woehrer et al. 2015).

Associated Literature

1. Ref:29021213: Noto T, Mochizuki K (2017) Whats, hows and whys of programmed DNA elimination in Tetrahymena. Open biology 7(10):
2. Ref:26688337: Kataoka K, Mochizuki K (2015) Phosphorylation of an HP1-like Protein Regulates Heterochromatin Body Assembly for DNA Elimination. Developmental cell 35(6):775-88
3. Ref:25588944: Woehrer SL, Aronica L, Suhren JH, Busch CJ, Noto T, Mochizuki K (2015) A Tetrahymena Hsp90 co-chaperone promotes siRNA loading by ATP-dependent and ATP-independent mechanisms. The EMBO journal 34(4):559-77
4. Ref:22103557: Mochizuki K (2012) Developmentally programmed, RNA-directed genome rearrangement in Tetrahymena. Development, growth & differentiation 54(1):108-19
5. Ref:21915788: Kataoka K, Mochizuki K (2011) Programmed DNA elimination in Tetrahymena: a small RNA-mediated genome surveillance mechanism. Advances in experimental medicine and biology 722( ):156-73
6. Ref:20211138: Noto T, Kurth HM, Kataoka K, Aronica L, DeSouza LV, Siu KW, Pearlman RE, Gorovsky MA, Mochizuki K (2010) The Tetrahymena argonaute-binding protein Giw1p directs a mature argonaute-siRNA complex to the nucleus. Cell 140(5):692-703

Sequences

>TTHERM_01276320(coding)
ATGTAAAGTTTTGTAAATCTTATCTAACTGGAAGAGAACTATAACTTAAGTCTTTTGTCT
TATTCAAACTCTATAAGGTAGTATCTATTAGATAACAAAGTGCACGAAGATAAATACATA
TAGCTCATGAAAGACATCAAAGATTTGTTTTCGTCTCCTACTTTTTTGATGCCAAATTTC
TCTGACAAAAGAAAATAATTTTTCTCATAATTATGCTTAATTGCTTTTGATTTTGCTAAT
AATTAAGTTACTGAATAATAATCTTTGAGTGAATTTAGAAAAAAATTCAAATTTCCTTTT
GTAATAATTGCTATTATTTACACTAAACTTTTAGAGATAAAAGAAGCCTTACCTGAAGAT
TTCTTAACTCTAGTGGATTGTCACTTCAAAAATAAAGGTCCTTAAAGTATATTTGCCTCT
TTGACATTGATTTCTTTATTTTTGAATGAAGTAGATTAATATGAAATAGATTGTGACCAT
ATTGAATCTTCAAATACATTAAGTAGTTGTAGCGAGGCTGCAGAGTCTAATATTCCTAAA
AAGGTCAATATAACTGTAAAGACAGTCTAAAATTTCTTTGATAAATAAGGATTATAGATG
ATAGCATTTCCCTTAAATGTTATCAAAAATATTCACTGTGTAGCACATGATCAATAGAGT
ATTATCCAGCTATTGCTAAAAATAGTTGTGATAATAATTAAAAACATGAGTAACAAAATC
AAATAAGAGTTTTTTTATTTTCATTAGAAGGATTTAAATGTGATTCTTTCATGTTTTATA
GGCTTTTTGTAGTAAAATAACTAGGCTAGAGAAGATCGCGATGTAGCTTTAGCAAATATT
TTTGAATTTTGCGAATTGCTAGCTAAATCTACTTCACCTGCTTAATTATACGTTGCGTTT
AAAGATTTTTTAGTTTATATTTCATAGAATTTTTCATTTGCTAATGATAGTATAGAATAC
TTTGACTTCAATTCTAAGTGTGTGGATTTTTTAGTTTCTATTACTCCATGCTTTATTTAA
TAGTCTGAGAATTTATCTGCATATCCTTTAATAATATAGTTATTGAAGTAATCTGCTAAA
ATAAATCTTGTGAATTTAGAGGATTAGATAGGCTGCTTGCATATATGGAGCCATTATTCT
GACTAGTTCATTCAGGCTTACGATAATAATAATAATTCAATAAAAGCTGAAACAATTAAT
AACTTAGCTATTCTGAATTCTACTTTTATTGAATCTCTACAATTTGATATAGAGCTATTA
TTTAATTTGTAGATAAAAGATCATGATTCTGAAGATGTTAATTAATTTGTGGACCAACTA
TCCCGCTTAGTTATTCATTCTAATCTCATTGATAACCAATTATTTGTAGAAGTAATAAAA
TCTTTGAGTCTACTTTATTAGAAAAATATGCTCCTTTATTATACTTTCCAAAAATGTTTG
ATGAAGTAGTACCTTAATTCTAAAGATACTCTCAGCTAAATTTACTAAGTTTTTGGCAAG
AGTAATGATTTTCTTTAGATTACAGAATTGTTATTTGAATCTTTTAGTTAAGCACAAAAA
TAATTTGCAAATTTAGATCTAAATACCATAGCTGCTATTGAAAAATTTACATAGTTCATG
TCTTAAATAGGTGCAAAGCTGTTTAATACTTAAATATCTCATCTTGAGTAGTCTTAAATT
TTGGGAATATTTCAAAAATATATTTTGAACCCAATTAAAATGTTTAATTTAAATATATTT
TCTTAATTACCTGAAAATTTTATACAGCTTAATGAAGTTTAATTTAGTCAAGTCTGCACA
ATGATGGAGTCAGTTCATTACTTTATTTTCTCTGACAATAAAAGCAATTCAGATTAAGTT
TTTGAGACAGAGGTTGATGTTTCTATGATAAAAAAGATATTGTTATTCAATTAATCAGTT
AACTAAGACATGGCCTACTGGATTAACCCACAATTAAAGAATGAAAGAAAAAAAATAAAA
TAATTCTACCAAAGTACAATTTATTGGGTAATATTTGCTTCAATTAATCTTGAAAATGCT
GCTAACATATTTGAAAGCTATTTCTTTTAGAAAGAGTTCAATATTTGGGTATTGAATTCT
ACTATCAAAAAGCTCAAAGGTAGGATATTACCAAACACATTTCTGCTTTACTTGTGTTAC
TATTTAAAATCCCTCACTGACTCTGATAGTTTTAGCAAAATTGTTTAAAAGAGTGAATAT
GAAGAAAAATTTAGAAAATTAATGGTAAATATTTCTAAATCTGTACCTTCTGAGATATAT
GTAGATTACTCTAAAATTTAGTACTATCTTTAATTATACATTAATGAAGAGTGTTAAATG
TTTAGATTTTAAATATTAAGTGACTAGACAGTCAGCTTACTGACATTAGAAAACAGATTT
TCTCATGTTTATAAAGTATTTATAATATTAGAAGATCTCTTTACAATTGTTTAGAGATTT
CCTAAGTTTTATTATGAAAATACATTTATGGGTCTAAAAAGGAATTTCTTTTTAAATCGT
GTTTTTTGGATTATTGTTAAATGCTTTACAATAGTTCCTAAGTAAGAGCTATTAGAAAAC
TCTCAGATCTATAAAACTATTTCTCTTTACTTAAATCTGGCAATTAGATTTAAAATGGAT
TTTCTTGAGAAAGAAGAAACTTAGTCAGAAAATTCTCACTATTTTAATTTCATTCTAGAG
ATATTAGACCACATGTATTTTACTAATAAAAAAATTGTGCTTGATTTTTGTTAAAAGATA
TTGAATTATTTTGATTAAGATTGCTATAATTAAAACATTATTTAATTAGCTTAATCTAGC
CAAACTTTTTAAAACATCACTCTTTAGCTTTTTACTAATATTTGGGAGTAAATCTTCAAT
TTTTGTTCTAGTAGCAATGAAAAGGCAGAAGAGTTTAAAGAAAAAGATATTAGCTAATCT
ATTGATTTTATTTTAAAATTCTCTAAGTGTTTATAAGGTAACTTGTCAATAATTTAAGAC
CGTATTATGCTTGTTATAAAAGCCCATCCTATTTATAGTTCGAATTCTCAAATTATTTAT
TCCACTATCATAGAAAATTTGGTAATTAAAGAAATAGATGGAGTTAAGGTTCTTTAAAAT
GTTTTTTTAAAATCTATAATTAAAGACTTACAGATTTGGGCTAAATCTATATGTCCTTAG
AGATTACAAAGCTGA

>TTHERM_01276320(gene)
TATTAAACTAAAAATTAGCTCATATTTTATTAAGTGATTATCACTTGTTTAAGGTTATTA
AATGAAGACTAATTATTTTAAAATTAATTATAATAATTAAATATTAAATTAGATTATTAC
CAACAGAATATTAAAGAAATGTAAAGTTTTGTAAATCTTATCTAACTGGAAGAGAACTAT
AACTTAAGTCTTTTGTCTTATTCAAACTCTATAAGGTAGTATCTATTAGATAACAAAGTG
CACGAAGATAAATACATATAGCTCATGAAAGACATCAAAGATTTGTTTTCGTCTCCTACT
TTTTTGATGCCAAATTTCTCTGACAAAAGAAAATAATTTTTCTCATAATTATGCTTAATT
GCTTTTGATTTTGCTAATAATTAAGTTACTGAATAATAATCTTTGAGTGAATTTAGAAAA
AAATTCAAATTTCCTTTTGTAATAATTGCTATTATTTACACTAAACTTTTAGAGATAAAA
GAAGCCTTACCTGAAGATTTCTTAACTCTAGTGGATTGTCACTTCAAAAATAAAGGTCCT
TAAAGTATATTTGCCTCTTTGACATTGATTTCTTTATTTTTGAATGAAGTAGATTAATAT
GAAATAGATTGTGACCATATTGAATCTTCAAATACATTAAGTAGTTGTAGCGAGGCTGCA
GAGTCTAATATTCCTAAAAAGGTCAATATAACTGTAAAGACAGTCTAAAATTTCTTTGAT
AAATAAGGATTATAGATGATAGCATTTCCCTTAAATGTTATCAAAAATATTCACTGTGTA
GCACATGATCAATAGAGTATTATCCAGCTATTGCTAAAAATAGTTGTGATAATAATTAAA
AACATGAGTAACAAAATCAAATAAGAGTTTTTTTATTTTCATTAGAAGGATTTAAATGTG
ATTCTTTCATGTTTTATAGGCTTTTTGTAGTAAAATAACTAGGCTAGAGAAGATCGCGAT
GTAGCTTTAGCAAATATTTTTGAATTTTGCGAATTGCTAGCTAAATCTACTTCACCTGCT
TAATTATACGTTGCGTTTAAAGATTTTTTAGTTTATATTTCATAGAATTTTTCATTTGCT
AATGATAGTATAGAATACTTTGACTTCAATTCTAAGTGTGTGGATTTTTTAGTTTCTATT
ACTCCATGCTTTATTTAATAGTCTGAGAATTTATCTGCATATCCTTTAATAATATAGTTA
TTGAAGTAATCTGCTAAAATAAATCTTGTGAATTTAGAGGATTAGATAGGCTGCTTGCAT
ATATGGAGCCATTATTCTGACTAGTTCATTCAGGCTTACGATAATAATAATAATTCAATA
AAAGCTGAAACAATTAATAACTTAGCTATTCTGAATTCTACTTTTATTGAATCTCTACAA
TTTGATATAGAGCTATTATTTAATTTGTAGATAAAAGATCATGATTCTGAAGATGTTAAT
TAATTTGTGGACCAACTATCCCGCTTAGTTATTCATTCTAATCTCATTGATAACCAATTA
TTTGTAGAAGTAATAAAATCTTTGAGTCTACTTTATTAGAAAAATATGCTCCTTTATTAT
ACTTTCCAAAAATGTTTGATGAAGTAGTACCTTAATTCTAAAGATACTCTCAGCTAAATT
TACTAAGTTTTTGGCAAGAGTAATGATTTTCTTTAGATTACAGAATTGTTATTTGAATCT
TTTAGTTAAGCACAAAAATAATTTGCAAATTTAGATCTAAATACCATAGCTGCTATTGAA
AAATTTACATAGTTCATGTCTTAAATAGGTGCAAAGCTGTTTAATACTTAAATATCTCAT
CTTGAGTAGTCTTAAATTTTGGGAATATTTCAAAAATATATTTTGAACCCAATTAAAATG
TTTAATTTAAATATATTTTCTTAATTACCTGAAAATTTTATACAGCTTAATGAAGTTTAA
TTTAGTCAAGTCTGCACAATGATGGAGTCAGTTCATTACTTTATTTTCTCTGACAATAAA
AGCAATTCAGATTAAGTTTTTGAGACAGAGGTTGATGTTTCTATGATAAAAAAGATATTG
TTATTCAATTAATCAGTTAACTAAGACATGGCCTACTGGATTAACCCACAATTAAAGAAT
GAAAGAAAAAAAATAAAATAATTCTACCAAAGTACAATTTATTGGGTAATATTTGCTTCA
ATTAATCTTGAAAATGCTGCTAACATATTTGAAAGCTATTTCTTTTAGAAAGAGTTCAAT
ATTTGGGTATTGAATTCTACTATCAAAAAGCTCAAAGGTAGGATATTACCAAACACATTT
CTGCTTTACTTGTGTTACTATTTAAAATCCCTCACTGACTCTGATAGTTTTAGCAAAATT
GTTTAAAAGAGTGAATATGAAGAAAAATTTAGAAAATTAATGGTAAATATTTCTAAATCT
GTACCTTCTGAGATATATGTAGATTACTCTAAAATTTAGTACTATCTTTAATTATACATT
AATGAAGAGTGTTAAATGTTTAGATTTTAAATATTAAGTGACTAGACAGTCAGCTTACTG
ACATTAGAAAACAGATTTTCTCATGTTTATAAAGTATTTATAATATTAGAAGATCTCTTT
ACAATTGTTTAGAGATTTCCTAAGTTTTATTATGAAAATACATTTATGGGTCTAAAAAGG
AATTTCTTTTTAAATCGTGTTTTTTGGATTATTGTTAAATGCTTTACAATAGTTCCTAAG
TAAGAGCTATTAGAAAACTCTCAGATCTATAAAACTATTTCTCTTTACTTAAATCTGGCA
ATTAGATTTAAAATGGATTTTCTTGAGAAAGAAGAAACTTAGTCAGAAAATTCTCACTAT
TTTAATTTCATTCTAGAGATATTAGACCACATGTATTTTACTAATAAAAAAATTGTGCTT
GATTTTTGTTAAAAGATATTGAATTATTTTGATTAAGATTGCTATAATTAAAACATTATT
TAATTAGCTTAATCTAGCCAAACTTTTTAAAACATCACTCTTTAGCTTTTTACTAATATT
TGGGAGTAAATCTTCAATTTTTGTTCTAGTAGCAATGAAAAGGCAGAAGAGTTTAAAGAA
AAAGATATTAGCTAATCTATTGATTTTATTTTAAAATTCTCTAAGTGTTTATAAGGTAAC
TTGTCAATAATTTAAGACCGTATTATGCTTGTTATAAAAGCCCATCCTATTTATAGTTCG
AATTCTCAAATTATTTATTCCACTATCATAGAAAATTTGGTAATTAAAGAAATAGATGGA
GTTAAGGTTCTTTAAAATGTTTTTTTAAAATCTATAATTAAAGACTTACAGATTTGGGCT
AAATCTATATGTCCTTAGAGATTACAAAGCTGATTTAATTTTTTTTTGTGTAATTATATT
AAATATATTTTTTACATTTTTATGTTTGTTTCCTATTATTCTATTAAACAAATTCAT

>TTHERM_01276320(protein)
MQSFVNLIQLEENYNLSLLSYSNSIRQYLLDNKVHEDKYIQLMKDIKDLFSSPTFLMPNF
SDKRKQFFSQLCLIAFDFANNQVTEQQSLSEFRKKFKFPFVIIAIIYTKLLEIKEALPED
FLTLVDCHFKNKGPQSIFASLTLISLFLNEVDQYEIDCDHIESSNTLSSCSEAAESNIPK
KVNITVKTVQNFFDKQGLQMIAFPLNVIKNIHCVAHDQQSIIQLLLKIVVIIIKNMSNKI
KQEFFYFHQKDLNVILSCFIGFLQQNNQAREDRDVALANIFEFCELLAKSTSPAQLYVAF
KDFLVYISQNFSFANDSIEYFDFNSKCVDFLVSITPCFIQQSENLSAYPLIIQLLKQSAK
INLVNLEDQIGCLHIWSHYSDQFIQAYDNNNNSIKAETINNLAILNSTFIESLQFDIELL
FNLQIKDHDSEDVNQFVDQLSRLVIHSNLIDNQLFVEVIKSLSLLYQKNMLLYYTFQKCL
MKQYLNSKDTLSQIYQVFGKSNDFLQITELLFESFSQAQKQFANLDLNTIAAIEKFTQFM
SQIGAKLFNTQISHLEQSQILGIFQKYILNPIKMFNLNIFSQLPENFIQLNEVQFSQVCT
MMESVHYFIFSDNKSNSDQVFETEVDVSMIKKILLFNQSVNQDMAYWINPQLKNERKKIK
QFYQSTIYWVIFASINLENAANIFESYFFQKEFNIWVLNSTIKKLKGRILPNTFLLYLCY
YLKSLTDSDSFSKIVQKSEYEEKFRKLMVNISKSVPSEIYVDYSKIQYYLQLYINEECQM
FRFQILSDQTVSLLTLENRFSHVYKVFIILEDLFTIVQRFPKFYYENTFMGLKRNFFLNR
VFWIIVKCFTIVPKQELLENSQIYKTISLYLNLAIRFKMDFLEKEETQSENSHYFNFILE
ILDHMYFTNKKIVLDFCQKILNYFDQDCYNQNIIQLAQSSQTFQNITLQLFTNIWEQIFN
FCSSSNEKAEEFKEKDISQSIDFILKFSKCLQGNLSIIQDRIMLVIKAHPIYSSNSQIIY
STIIENLVIKEIDGVKVLQNVFLKSIIKDLQIWAKSICPQRLQS